scholarly journals Integrative taxonomy supports the presence of two species of Kyphosus (Perciformes: Kyphosidae) in Atlantic European waters

2017 ◽  
Vol 81 (4) ◽  
pp. 467 ◽  
Author(s):  
Rafael Bañón ◽  
David Barros-García ◽  
Alejandro De Carlos
Keyword(s):  
New Record ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Integrative Taxonomy ◽  
Index Number ◽  
Dna Barcodes ◽  
Northwestern Spain ◽  
Kyphosus Vaigiensis ◽  
Coi Sequences ◽  
Public Repositories

The taxonomic identification of one Kyphosus sectatrix and two Kyphosus vaigiensis specimens caught in the European Atlantic waters of Galicia, northwestern Spain, was carried out by means of integrative taxonomy, combining the examination of morphological characters and DNA barcodes. Taxonomical assignation based on morphological characters of these specimens was tested by comparing their COI sequences with available data of Kyphosus deposited in public repositories. The resulting neighbour-joining tree defined four clades corresponding to Barcode Index Number (BIN) and indicated that some nucleotide sequences from Kyphosus, previously deposited, probably originate from misidentified specimens, as would be expected from cryptic and sympatric species. The specimens of Kyphosus vaigiensis represent a new record for the waters of Galicia and the northernmost record in the eastern Atlantic. This kind of herbivorous tropical fishes could play an important role in the tropho-dynamic context of this temperate coastal ecosystem.

scholarly journals A new record of odd-scaled snake (Serpentes, Xenodermidae) from Vietnam: expanded description of Parafimbrios vietnamensis based on integrative taxonomy

ZooKeys ◽  
2021 ◽  
Vol 1048 ◽  
pp. 79-89
Author(s):  
Nikolai L. Orlov ◽  
Oleg A. Ermakov ◽  
Tao Thien Nguyen ◽  
Natalia B. Ananjeva
Keyword(s):  
Genetic Divergence ◽  
New Record ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Type Locality ◽  
Morphological Data ◽  
Snake Species ◽  
Male Individual

Based on the combination of molecular and morphological data, we herein report the second known finding of the xenodermid snake species Parafimbrios vietnamensis Ziegler, Ngo, Pham, Nguyen, Le & Nguyen, 2018. The male individual was found in the Yen Bai Province of northwestern Vietnam, more than 200 km from the type locality in Lai Chau Province. Genetic divergence between the newly-collected male and the holotype was low (1.7%), and is in agreement with morphological data that supports that they are conspecific. We give a detailed description of the morphological characters and coloration of the new record and provide an expanded diagnosis of P. vietnamensis. Parafimbrios is a poorly-understood genus, and our recent discovery brings the total number of known specimens of the genus to nine, 1/3 of them having been found in Vietnam (one specimen of P. lao and now two specimens of P. vietnamensis).

scholarly journals Perlidae (Plecoptera) from the Paranapiacaba Mountains, Atlantic Forest, Brazil: Diversity and implications of the integrative approach and teneral specimens on taxonomy

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243393
Author(s):  
Lucas Henrique de Almeida ◽  
Pitágoras da Conceição Bispo
Keyword(s):  
New Record ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Coi Gene ◽  
Southeastern Brazil ◽  
Integrative Approach ◽  
South American ◽  
Neighbor Joining ◽  
Previous Record ◽  
Coi Sequences

The study of complementary sources of biological variation (e.g. morphological, molecular) has allowed a better understanding of biodiversity through the construction of an integrative taxonomy. Using this approach, specimens from the Paranapiacaba Mountains, southeastern Brazil, were studied to update the knowledge on the stonefly family Perlidae from the region, characterize the species, and make associations between nymphs and adults using a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene. The study also discusses the implications of integrative taxonomy and teneral specimens for the study of South American Perlidae. The molecular data were analyzed using Bayesian inference, Neighbor-joining, and delimiting species methods. Our results revealed that, in general, there was a morphological and molecular congruence between species. In the Paranapiacaba Mountains, three genera and 15 species were recorded: Anacroneuria boraceiensis Froehlich 2004, A. debilis (Pictet 1841) (new record), A. fiorentini De Ribeiro and Froehlich 2007 (new record), A. flintorum Froehlich 2002, A. iporanga Bispo and Froehlich 2004, A. itajaimirim Bispo and Froehlich 2004, A. polita (Burmeister 1913), A. subcostalis Klapálek 1921, A. tupi Bispo and Froehlich 2004 (with a description of the nymph), Kempnyia auberti Froehlich 1996, K. colossica (Navás 1934), K. flava Klapálek 1916, K. neotropica (Jacobson and Bianchi 1905) (including its new junior synonym K. petersorum Froehlich 1996), Kempnyia sp., and Macrogynoplax veneranda Froehlich 1984. COI sequences were obtained for 11 species, five of which had nymphs associated with adults. Among the five associated nymphs, the nymph of A. tupi is described here. The results of this study indicate that the color of adult teneral specimens differs from that of mature specimens. Given this, the synonym of K. neotropica and K. petersorum was proposed since these species have high morphological and molecular similarities and differ only in color patterns. In addition, the previous record of A. petersi Froehlich 2002 from the Paranapiacaba Mountains was invalidated since it was considered a teneral specimen of A. flintorum. These results suggest that the development of an integrative taxonomy is essential to continue advancing the study of Perlidae diversity in South America.

scholarly journals The first molecular insight into the genus Turanium Baeckmann, 1922 (Coleoptera: Cerambycidae: Callidiini) with a description of a new species from Middle Asia

2021 ◽  
Vol 79 ◽  
pp. 465-484
Author(s):  
Lech Karpiński ◽  
Wojciech T. Szczepański ◽  
Radosław Plewa ◽  
Lech Kruszelnicki ◽  
Katarzyna Koszela ◽  
...  
Keyword(s):  
New Species ◽  
Strong Support ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Central Asian ◽  
Ecological Associations ◽  
Coi Sequences ◽  
A New Species ◽  
Insight Into ◽  
Key Characters

This paper sheds the first light on the phylogeny of the Central Asian genus Turanium Baeckmann, 1922. By applying an integrative taxonomy approach, we revealed and described a new species from Kyrgyzstan—Turanium losi Karpiński, Plewa & Hilszczański sp. nov. Distinguishing characters from closely related Turanium pilosum (Reitter, 1891) are presented and their ecological associations are discussed. The key characters, including the male terminalia, were examined by means of scanning electron microscopy. High-quality stacked photographs of the habitus of the specimens are presented for both species and their geographical distributions are mapped. While the new species shows stable morphological characters that allow its differentiation from T. pilosum and the COI genetic distance between them is approx. 3%, the different species delimitation methods gave discordant results. Although the new species remained unrecognized for so long, it seems that these cerambycids are common in the region and both can be considered potentially invasive as they are apparently highly polyphagous. It has also been documented that they occur sympatrically in Kyrgyzstan. Both the Bayesian and maximum likelihood analyses of COI sequences confirmed the monophyly of the genus Turanium with strong support (PP 1 and BS 90, respectively). Moreover, the recently revealed polyphyly of the tribe Callidiini was supported by our analyses and, consequently, the discussion on the establishment of a new tribe Ropalopini is raised. This study further corroborates the effectiveness of DNA barcoding as a tool in detecting new species and provides some of the first sequences for Central Asian cerambycids, which remain almost completely unknown in terms of molecular studies.

Six species in one: evidence of cryptic speciation in the Hygrobates fluviatilis complex (Acariformes, Hydrachnidia, Hygrobatidae)

2017 ◽  
Vol 22 (9) ◽  
pp. 1327 ◽  
Author(s):  
Vladimir Pešić ◽  
Mahdieh Asadi ◽  
Mirela Cimpean ◽  
Miroslawa Dabert ◽  
Yunus Esen ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Morphological Characters ◽  
Morphological Data ◽  
Cryptic Speciation ◽  
Dna Barcodes ◽  
Water Mites ◽  
Additional Species ◽  
Biogeographic Regions ◽  
Coi Sequences ◽  
The Antarctic

Water mites of the genus Hygrobates are widely distributed in all biogeographic regions except the Antarctic. Palaearctic Hygrobates species with reticulated soft integument generally have been considered as representatives of one common and widely distributed species, Hygrobates fluviatilis Strøm, 1768. Based on partial COI sequences (DNA-barcodes) and statistical analysis of morphological data, we show that these mites belong to six distinct lineages. Two of them are widely distributed in Central Europe: Hygrobates fluviatilis here redescribed based on a neotype designated from the type locality in Norway, and a species new to science, H. arenarius Smit & Pešić. The four remaining lineages represent additional species new to science that appear to have more restricted distributions: H. corsicus Pešić & Smit (Corsica, Sardinia), H. marezaensis Pešić & Dabert (Montenegro, Albania, Croatia), H. turcicus Pešić, Esen & Dabert (Turkey), and H. persicus Pešić & Asadi (Iran, E Turkey). Statistical morphometric analysis reveals that the latter two species cannot be separated on morphological characters and should be considered true cryptic species. We provide data concerning biology and geographical distributions together with a key to all species of the complex.

Morphology and DNA barcodes of two species of Bradysia Winnertz from China (Diptera, Sciaridae), with the description of Bradysia minorlobus Yang, Shi & Huang sp. n.

Zootaxa ◽  
2019 ◽  
Vol 4612 (1) ◽  
pp. 85 ◽  
Author(s):  
XUE YANG ◽  
KAI SHI ◽  
KAI HELLER ◽  
FRANK MENZEL ◽  
JUNHAO HUANG ◽  
...  
Keyword(s):  
Morphological Characters ◽  
Species Group ◽  
Genetic Distances ◽  
Species Concepts ◽  
Similar Species ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
Coi Sequences ◽  
Morphological Species ◽  
Group Species

Two morphologically similar species of the fungicola species group of Bradysia Winnertz, 1867 were studied in China: Bradysia chenjinae Yang, Zhang & Yang, 1993 and Bradysia minorlobus Yang, Shi & Huang sp. n. The morphological species concepts were supported by the DNA barcodes of COI sequences. The genetic distances of 16 Bradysia fungicola group species were analyzed and a neighbor-joining tree was constructed. The morphological characters of both Chinese species were described and illustrated. 

Integrative taxonomy of Mexican cycads: biogeography, morphology and DNA barcoding corroborate a new sympatric species in Ceratozamia (Zamiaceae)

Phytotaxa ◽  
2016 ◽  
Vol 268 (1) ◽  
pp. 25 ◽  
Author(s):  
LILÍ MARTÍNEZ-DOMÍNGUEZ ◽  
FERNANDO NICOLALDE-MOREJÓN ◽  
FRANCISCO VERGARA-SILVA ◽  
DENNIS W. STEVENSON
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Sympatric Species ◽  
Integrative Taxonomy ◽  
Los Tuxtlas ◽  
A New Species

A new species, from the montane region of Los Tuxtlas (state of Veracruz, Mexico), Ceratozamia subroseophylla, is proposed in accordance with the integrative taxonomy framework and through application of the taxonomic circle rationale. Our identification of this new species is the result of a thorough re-evaluation of four formerly described Ceratozamia species, namely: (1) C. miqueliana, with which it overlaps geographically; (2) C. mexicana; (3) C. robusta, which has also been previously seen as morphologically similar to C. miqueliana; and (4) C. brevifrons, which was at the center of a controversy concerning the morphological and geographic limits when compared to C. mexicana. Even though there are morphometric affinities of C. subroseophylla with this set of species, there are apomorphies in qualitative morphological characters and diagnostic nucleotidic variants established through character-based DNA barcoding to allow the recognition of this new species. The implications that our integrative taxonomy-based assessment of systematic diversity in Ceratozamia species from Veracruz have for evaluating the overall taxonomic status of the genus are discussed.

scholarly journals Defining species boundaries in the Merodon avidus complex (Diptera, Syrphidae) using integrative taxonomy, with the description of a new species

2016 ◽  
Author(s):  
Jelena Ačanski ◽  
Ante Vujić ◽  
Mihajla Djan ◽  
Dragana Obreht Vidaković ◽  
Gunilla Ståhls ◽  
...  
Keyword(s):  
Sibling Species ◽  
Morphological Characters ◽  
Integrative Taxonomy ◽  
Coi Gene ◽  
Species Boundaries ◽  
Geometric Morphometric ◽  
Molecular Analyses ◽  
Mtdna Coi ◽  
The Status ◽  
Coi Sequences

Several recent studies have detected and described complexes of cryptic and sibling species in the genus Merodon (Diptera, Syrphidae). One representative of these complexes is the Merodon avidus complex that contains four sibling species, which have proven difficult to distinguish using traditional morphological characters. In the present study, we use two geometric morphometric approaches, as well as molecular characters of the 5’-end of the mtDNA COI gene, to delimit sibling taxa. Analyses based on these data were used to strengthen species boundaries within the complex, and to validate the status of a previously-recognized cryptic taxon from Lesvos Island (Greece), here described as Merodon megavidus Vujić & Radenković sp. nov. Geometric morphometric results of both wing and surstylus shape confirm the present classification for three sibling species－M. avidus (Rossi, 1790), M. moenium Wiedemann in Meigen, 1822 and M. ibericus Vujić, 2015－and, importantly, clearly discriminate the newly-described taxon Merodon megavidus sp. nov. In addition to our geometric morphometric results, supporting characters were obtained from molecular analyses of mtDNA COI sequences, which clearly differentiated M. megavidus sp. nov. from the other members of the M. avidus complex. Molecular analyses revealed that the earliest divergence of M. ibericus occurred around 800 ky BP, while the most recent separation happened between M. avidus and M. moenium around 87 ky BP.

scholarly journals Mining bee Andrena (Agandrena) agilissima (Hymenoptera: Andrenidae): A new record from India with morphological and molecular notes

2016 ◽  
Vol 8 (4) ◽  
pp. 1775-1778
Author(s):  
Gurpreet Singh Makkar ◽  
Debjani Dey ◽  
Pardeep K. Chhuneja
Keyword(s):  
New Record ◽  
Habitat Management ◽  
Compound Eye ◽  
Dna Barcode ◽  
Morphological Characters ◽  
Molecular Characteristics ◽  
Head Width ◽  
Index Number ◽  
Mean Values ◽  
First Time

The mining bee Andrena agilissima (Scopoli, 1770), is recorded for the first time in India from the western agro-climatic zone of its Punjab state. This is the first account of morphological and molecular characteristics of A. agilissima. This new record now increases the number of mining bees known in India to 21. Taxonomic commentsand metric values of 40 morphological characters have been presented. The mean values for body length, head width, compound eye length, median ocellus diameter, forewing length and hamuli number were 14.04±0.04 mm, 4.26±0.003 mm, 2.327±0.008 mm, 0.255±0.005 mm, 12.75±0.022 mm and 17.00±0.00, respectively. Using thestandard barcoding protocols, cytochrome c oxidase subunit 1 marker (standard DNA barcode region) based 658 bp DNA barcode sequence of the species has been established, as a first step towards the DNA barcode library of solitary bees of Punjab. The barcode sequence generated for the species has been registered by Gen- Bank, National Centre for Biotechnology Information (NCBI) under accession ‘KT960836’ and Barcode of Life Data (BOLD) Systems under Barcode Index Number ‘BOLD:AAY6909’. The floral sources for A. agilissima in Punjab are also provided. The results can be used to further study the races/ecotypes in different parts of country, habitat management studies, plant-pollinator interactions and in conservation programmes for the species. Further, the precise identification of A. agilissima and the inventory of its foraging plants would provide new opportunities for its potential use as pollinator of crops.

scholarly journals Overview of the Genetic Diversity of African Macrotermes (Termitidae: Macrotermitinae) and Implications for Taxonomy, Ecology and Food Science

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 518
Author(s):  
Bronwyn Egan ◽  
Zwannda Nethavhani ◽  
Barbara van Asch
Keyword(s):  
Negative Impact ◽  
Phylogenetic Reconstruction ◽  
Morphological Characters ◽  
Food Science ◽  
Cryptic Diversity ◽  
African Species ◽  
Genetic Groups ◽  
Wide Range ◽  
Coi Sequences ◽  
High Level

Macrotermes termites play important ecological roles and are consumed by many communities as a delicacy and dietary complement throughout Africa. However, lack of reliable morphological characters has hampered studies of Macrotermes diversity in a wide range of scientific fields including ecology, phylogenetics and food science. In order to place our preliminary assessment of the diversity of Macrotermes in South Africa in context, we analysed a comprehensive dataset of COI sequences for African species including new and publicly available data. Phylogenetic reconstruction and estimates of genetic divergence showed a high level of incongruity between species names and genetic groups, as well as several instances of cryptic diversity. We identified three main clades and 17 genetic groups in the dataset. We propose that this structure be used as a background for future surveys of Macrotermes diversity in Africa, thus mitigating the negative impact of the present taxonomic uncertainties in the genus. The new specimens collected in Limpopo fell into four distinct genetic groups, suggesting that the region harbours remarkable Macrotermes diversity relative to other African regions surveyed in previous studies. This work shows that African Macrotermes have been understudied across the continent, and that the genus contains cryptic diversity undetectable by classic taxonomy. Furthermore, these results may inform future taxonomic revisions in Macrotermes, thus contributing to advances in termitology.

Baetis majus sp. nov., new species of mayfly (Ephemeroptera: Baetidae) from Far East of Russia

Zootaxa ◽  
2021 ◽  
Vol 4965 (3) ◽  
pp. 541-557
Author(s):  
TATIANA M. TIUNOVA ◽  
ALEXANDER A. SEMENCHENKO ◽  
XIAOLI TONG
Keyword(s):  
New Species ◽  
Russian Far East ◽  
Far East ◽  
Morphological Characters ◽  
Species Group ◽  
Morphological Studies ◽  
Nearest Neighbours ◽  
Differential Identification ◽  
Coi Sequences ◽  
Western Palaearctic

A new species, Baetis majus Tiunova sp. nov., is described and illustrated based on larvae and reared adults discovered in the Russian Far East. The differential identification of this species was determined by the characteristics of other representatives of the genus Baetis Leach, including subgenera Baetis Leach and Tenuibaetis Kang & Yang from Eastern and Western Palaearctic, Nearctic and Oriental regions. In addition to morphological studies, DNA barcoding of the described species with average intraspecific K2P distances to nearest neighbours is documented. We reconstructed the phylogenetic relationships of all available cytochrome c oxidase subunit I (COI) sequences of the subgenera of Baetis and Tenuibaetis from four regions. Bayesian analysis using 47 morphological characters additional to partial COI sequences did not allow to determine the species-group of the Baetis genus to which the described species belongs. 

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