scholarly journals Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

2018 ◽  
Vol 12 (05) ◽  
pp. 305-312 ◽  
Author(s):  
Hafeza Aftab ◽  
Yoshio Yamaoka ◽  
Faruque Ahmed ◽  
Azad AK Khan ◽  
Phawinee Subsomwong ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Sensitivity And Specificity ◽  
Diagnostic Tests ◽  
Age Groups ◽  
Endoscopic Examination ◽  
Helicobacter Pylori Infection ◽  
Predictive Values ◽  
Urease Test ◽  
H Pylori

Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. We conducted a cross-sectional study to compare five diagnostic tests for H. pylori infection and studied the epidemiology of the infection in Bangladesh. Methodology: Bangladeshi patients with dyspeptic symptoms referred for endoscopic examination were enrolled in this study. Each patient underwent upper endoscopic examination and four gastric biopsy specimens were taken. We used 5 tests for the diagnosis of H. pylori; culture, histology confirmed by immunohistochemistry, rapid urease test (RUT), urinary and serological test. Demographic and environmental variables were collected. Results: A total of 133 patients participated in the study, 61 males and mean age 37.3 ± 12.3 years. We used the culture and/or histology results as the gold standard to estimate the sensitivity, specificity, positive and negative predictive values for the studied diagnostic tests. RUT, culture and histology had high sensitivity and specificity with moderate positive and negative likelihood ratio, whereas urine test and serology showed a good sensitivity and specificity but poor likelihood ratio. The overall prevalence of H. pylori among study subjects was 47% with no difference between gender and age groups. Conclusions: The invasive tests showed better performance than noninvasive tests among Bangladeshi population. The overall prevalence of H. pylori was less than the previously reported in the region with no difference among all age groups.

scholarly journals DIAGNOSTIC ACCURACY OF UREA BREATH TEST FOR HELICOBACTER PYLORI INFECTION IN CHILDREN WITH DYSPEPSIA IN COMPARISON TO HISTOPATHOLOGY

2016 ◽  
Vol 53 (2) ◽  
pp. 108-112 ◽  
Author(s):  
Naser HONAR ◽  
Alireza MINAZADEH ◽  
Nader SHAKIBAZAD ◽  
Mahmood HAGHIGHAT ◽  
Forough SAKI ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Carbon Isotope ◽  
Sensitivity And Specificity ◽  
Breath Test ◽  
Close Association ◽  
Urea Breath Test ◽  
Helicobacter Pylori Infection ◽  
Cross Sectional ◽  
Predictive Values ◽  
H Pylori

ABSTRACT Background - Helicobacter pylori infection is the gram negative bacillus with the close association with chronic antral gastritis. Objective - In this study, we evaluate the accuracy of urea breath test (UBT) with carbon isotope 13 in comparison with histopathology of gastric antrum for detection of H. pylori infection in children with dyspepsia. Methods - This cross-sectional study was performed at specialized laboratory of Shiraz Gastroenterohepatology Research Center and Nemazee Hospital, Iran, during a 12-months period. This study investigated the sensitivity, specificity, and positive and negative predictive values of UBT in comparison with biopsy-based tests. We included a consecutive selection of 60 children who fulfilled Rome III criteria for dyspepsia. All children were referred for performing UBT with carbon isotope 13 (C13) as well as endoscopy. Biopsies were taken from antrum of stomach and duodenum. The pathologic diagnosis was considered as the standard test. Results - The mean age of the participants was 10.1±2.6 (range 7-17 years). From our total 60 patients, 28 (46.7%) had positive UBT results and 32 (53.3%) had negative UBT results. Pathologic report of 16 (57.1%) out of 28 patients who had positive UBT were positive for H. pylori and 12 (42.9%) ones were negative. Sensitivity and specificity of C13-UBT for detection of H. pylori infection were 76.2% and 69.2% respectively. Conclusion - Sensitivity and specificity of C13-UBT for detection of H. pylori infection were 76.2% and 69.2% respectively. Another multicenter study from our country is recommended.

scholarly journals Comparison of Serum IgG Antibody Test with Gastric Biopsy for the Detection of Helicobacter Pylori Infection among Egyptian Children

2015 ◽  
Vol 3 (2) ◽  
pp. 303-306 ◽  
Author(s):  
Mones M. Abu Shady ◽  
Hanan A. Fathy ◽  
Alaa Ali ◽  
Essam M. Galal ◽  
Gihan A. Fathy ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Serological Test ◽  
Standard Test ◽  
Helicobacter Pylori Infection ◽  
Igg Antibody ◽  
Serum Igg ◽  
Egyptian Children ◽  
Urease Test ◽  
H Pylori

BACKGROUND: In developing countries, Helicobacter pylori (H. pylori) infection is mainly acquired during childhood and may be a predisposing factor for peptic ulcer or gastric cancer later in life. Noninvasive diagnostic tools are particularly useful in children for screening tests and epidemiological studies. Data on serologic testing of children are lacking. Accurate noninvasive tests for diagnosing Helicobacter pylori infection in children are strongly required.AIM: The aim of this study was to evaluate the performance of a serological test (serum IgG antibody for H. pylori) in Egyptian children with recurrent abdominal pain necessitating endoscopy.SUBJECTS AND METHODS: One hundred children, referred to the endoscopy unit at Mansoura University. Upper endoscopy was done for each with rapid urease test (RUT) and histological examination as the gold standard test for detection of H. pylori infection. Serum samples were collected for detecting IgG for H. pylori infection.RESULTS: The mean age of the subjects included in the study was 7.23 ± 1.94 year. Serological test (IgG to H. pylori) was positive in 60% of all cases. A highly significant association between the standard test and the serological test at a cutoff > 10 U/ml at p = 0.001 were detected for the diagnosis of H. pylori infection. The sensitivity, specificity, positive likelihood ratio, and negative likelihood ratio for the IgG antibody a cutoff > 10 U/ml, were 96.5%, 93%, 13.83, 0.038 respectively.CONCLUSION: Serum IgG antibody to H. pylori infection has a high diagnostic value and can be considered as a suitable and reliable noninvasive test for detection of H. pylori infection.

Good diagnostic accuracy of a chemiluminescent immunoassay in stool samples for diagnosis of Helicobacter pylori infection in patients with dyspepsia

2016 ◽  
Vol 64 (2) ◽  
pp. 388-391 ◽  
Author(s):  
María José Ramírez-Lázaro ◽  
Josep Lite ◽  
Sergio Lario ◽  
Pepa Pérez-Jové ◽  
Antònia Montserrat ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gold Standard ◽  
Rapid Urease Test ◽  
Diagnostic Reliability ◽  
Predictive Values ◽  
Urease Test ◽  
Stool Samples ◽  
H Pylori ◽  
Good Diagnostic Accuracy ◽  
Sensitivity Specificity

Laboratory-based chemiluminescence immunoassays (CLIA) are widely used in clinical laboratories. Some years ago, a CLIA test was developed for the detection of Helicobacter pylori in stool samples, known as LIAISON H. pylori SA, but little information on its use has been reported. To evaluate the accuracy of the LIAISON H. pylori SA assay for diagnosing H. pylori infection prior to eradication treatment. Diagnostic reliability was evaluated in 252 untreated consecutive patients with dyspepsia. The gold standard for diagnosing H. pylori infection was defined as the concordance of the rapid urease test (RUT), histopathology and urea breath test (UBT). The CLIA assay was performed according to the manufacturer's instructions. Sensitivity, specificity, positive and negative predictive values, and 95% CIs were calculated. According to the gold standard selected, 121 patients were positive for H. pylori infection and 131 negative. LIAISON H. pylori SA had a sensitivity of 90.1% and a specificity of 92.4%, with positive and negative predictive values of 91.6% and 90.1%, respectively. The accuracy of the LIAISON H. pylori SA chemiluminescent diagnostic assay seems comparable to that of ELISA or the best-performing LFIAs. Its sensitivity and specificity, however, seem slightly lower than those of histology, RUT or UBT. The advantages of the assay are that it is cheap, automated, and minimally labor-intensive.

scholarly journals TIME TRENDS OF HELICOBACTER PYLORI PREVALENCE IN ITAJAÍ - SC: A RETROSPECTIVE STUDY OF 25 YEARS BASED ON ENDOSCOPIC DATABASE

2019 ◽  
Vol 56 (1) ◽  
pp. 10-14 ◽  
Author(s):  
Fangio FERRARI ◽  
Elisa Cantú Germano DUTRA ◽  
Henrieli Correia ZANARDI ◽  
Bruno Lorenzo SCOLARO ◽  
Odemari Miranda FERRARI
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Developed Countries ◽  
Helicobacter Pylori Infection ◽  
Age Group ◽  
Gradual Reduction ◽  
Urease Test ◽  
Significant Difference ◽  
The Mean ◽  
And Gender

ABSTRACT BACKGROUND: The bacterium Helicobacter pylori is strongly associated with the development of gastric adenocarcinoma. Currently, the prevalence in developed countries is 40%, but this value increases considerably in developing countries, which can reach rates bigger than 90%. OBJECTIVE: The objective of this study was to determine the mean and annual prevalence of Helicobacter pylori infection in patients from Itajaí during the period from July 1992 to April 2016, as well as the gender and age groups most affected. METHODS: After consent of the clinical director of the Gastroclinica Itajaí and confidentiality commitment about the research, the database of the Endoscopy Service of the clinic was evaluated. All the patients who underwent their first upper digestive endoscopy with urease test and/or histological analysis were included. The data were submitted to statistical analysis of prevalence by gender, age group and years of study, with subsequent correction through the confidence interval. RESULTS: The mean prevalence of Helicobacter pylori infection thru all years of study was 50.07%. With the calculation of the annual prevalences, it was evident the gradual reduction of infection in the population of Itajaí, that was 81.3% in 1992, declining to 33% in the year of 2016. When classifying the prevalence of infection by gender, it was higher in males (53.59%), and gender distribution by age group showed no statistically significant difference among genders between the ages of 40 and 80 years. In relation to the age group, the highest prevalence was in the group between 40 and 49 years. CONCLUSION: Although this study is retrospective and based on endoscopic database analysis, without access to clinical data of patients such as prior use of proton pump inhibitor and antibiotics to endoscopy, its results are important because they may reflect the current panorama of Helicobacter pylori infection in the city under study, where it has been presenting a gradual reduction of prevalence over the years, with current rates similar to that of developed countries (33%). Future studies are needed to confirm our data.

scholarly journals Diagnostic Techniques and Antimicrobial Susceptibility Test of Helicobacter Pylori Infection in Gashua Town

2019 ◽  
Vol 15 (10) ◽  
pp. 126
Author(s):  
A.G. Dagona ◽  
I.G Dagona ◽  
K Muhammad ◽  
F.A. Garba ◽  
W.D Taura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Likelihood Ratio ◽  
Antimicrobial Susceptibility ◽  
Accurate Diagnosis ◽  
Test Method ◽  
Susceptibility Test ◽  
Antimicrobial Susceptibility Test ◽  
Noninvasive Methods ◽  
Urease Test ◽  
H Pylori

<p class="0abstract">Helicobacter pylori eradication continues to be a challenge in a group of patients after the failure of several therapeutic regimen attempts. Treatment regimen is generally chosen on the basis of the prevalence of bacterial resistance detected against the tested antibiotics. 60 sixty  (60), adult out patients presented to Gastroenterology clinic of General Hospital Gashua undertook multiple gastric biopsy and specimens were carried for gram stain, culture, antimicrobial sensitivity testing, rapid urease test and histology. Antimicrobial susceptibility test were carryout using Epsilometer testing Kit (E-test) method and tested against Omeprazole, metronidazole Ranitidine, and Amoxicillin. About Sixty percent (30%) of the study population was positive for H. pylori infection (mean age of 34 years ± 15), 60% were males while 40% are women which are mostly married. Helicobacter pylori culture showed a sensitivity of 55% (95% CI (29.5– 62.1), specificity of 97% (95%CI (80.5–100%), positive likelihood ratio of 19.93 (95% CI (1.254– 317.04) and a negative likelihood ratio of 0.76 (95% CI (0.406–0.772). Eighteen (18) strains of Helicobacter pylori isolated 14 are sensitive to Amoxicillin, Ciprofloxacin, metronidazole; amoxicillin three (4) strains were resistant to ciprofloxacin and Amoxicillin and metronidazole when tested. It is imperative to have indebted knowledge on diagnostic method and antibiotic susceptibility patterns in the study area, since the accurate diagnosis of H. pylori is the idealistic view for both gastroenterologists and microbiologists, using synergistically invasive and noninvasive methods will be a future challenge in medical research topics. It is clear that recent advances in invasive and noninvasive methods for accurate diagnosis of the H. pylori can drastically change upcoming guidelines attributed with the management of this infection.</p>

scholarly journals European Registry on the management of Helicobacter pylori infection: features of diagnosis and treatment in Kazan

2020 ◽  
Vol 92 (8) ◽  
pp. 52-59
Author(s):  
S. R. Abdulkhakov ◽  
D. S. Bordin ◽  
R. A. Abdulkhakov ◽  
D. D. Safina ◽  
A. R. Gizdatullina ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Proton Pump Inhibitor ◽  
Triple Therapy ◽  
Proton Pump ◽  
Eradication Therapy ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Standard Triple Therapy ◽  
Urease Test ◽  
H Pylori

Background. As part of an observational multicenter prospective study European Registry on the management of Helicobacter pylori infection, conducted on the initiative of the European H. pylori and Microbiota Study Group, the compliance of clinical practice in the management of patients with Helicobacter pylori infection in Kazan with clinical guidelines was assessed. Materials and methods. The data of 437 patients included into the register by clinical sites in Kazan in 20132019 were analyzed. The methods used for the initial diagnosis of H. pylori infection and eradication control were evaluated. The frequency of various eradication therapy regimens prescription was analyzed in 379 cases. Data regarding the effectiveness of eradication therapy was analyzed in 173 patients. Results. The rapid urease test (44.2% of cases) and cytology/histology (60% of cases) were most often used for the initial diagnosis of H. pylori infection; however non-invasive methods such as 13C-urea breath (9.2%), serology (6.2%), H. pylori stool antigen test (2.3%) were less common. In 21.7% of patients two methods of H. pylori detection were used for primary diagnosis. The control test to evaluate the effectiveness of eradication therapy at the recommended timepoint was performed in 46.2% of patients. 13C-urea breath test (31.7%), stool PCR/stool antigen test (28.7%), rapid urease test (22.3%), cytology/histology (26.2% of cases) prevailed in the assessment of eradication rate. Standard triple therapy, including proton pump inhibitor, clarithromycin and amoxicillin was most commonly prescribed as first-line therapy (64.6% of cases). The duration of eradication therapy was 14 days in the majority of cases with pantoprazole as the most common proton pump inhibitor in standard triple therapy regimens (84.8%). The efficacy of 14-day standard triple therapy (mITT) was 87.0%. Conclusion. The results indicate a high frequency of non-invasive methods use for assessing the effectiveness of eradication therapy; however, the overall rate of eradication efficacy assessment is low, limiting the possibility of analyzing the eradication results. The effectiveness of the most common 14-day standard triple first-line therapy in Kazan doesnt reach the recommended 90% eradication level. This could be explained by high rate of pantoprazole use, which is not an optimal proton pump inhibitor in eradication therapy regimens.

scholarly journals ASSOCIATION OF DUODENAL EOSINOPHILIC INFILTRATE WITH HELICOBACTER PYLORI INFECTION, BUT NOT WITH FUNCTIONAL DYSPEPSIA

2020 ◽  
Vol 57 (1) ◽  
pp. 74-78 ◽  
Author(s):  
Carine LEITE ◽  
Luiz Edmundo MAZZOLENI ◽  
Diego de Mendonça UCHOA ◽  
Juliana Araújo CASTANHO ◽  
Felipe MAZZOLENI ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Functional Dyspepsia ◽  
Eosinophil Count ◽  
Gastrointestinal Symptoms ◽  
Duodenal Mucosa ◽  
Helicobacter Pylori Infection ◽  
Eosinophilic Infiltrate ◽  
Urease Test ◽  
H Pylori

ABSTRACT BACKGROUND: The role of Helicobacter pylori infection on eosinophilic infiltration in duodenal mucosa is poorly studied. An increase in the number of eosinophils in duodenum has been associated with functional dyspepsia. OBJECTIVE: To evaluate the influence of H. pylori infection on duodenal eosinophil count and the role of eosinophilic infiltrate of duodenum in functional dyspepsia. METHODS: Positive and negative H. pylori individuals were included. Both functional dyspeptic patients according to Rome III criteria (cases) and individuals without gastrointestinal symptoms (controls) were enrolled. They were submitted to upper endoscopy and H. pylori infection was verified by gastric histopathology and urease test. Eosinophils in the duodenal mucosa were counted in five high-power fields, randomly selected on slides of endoscopic biopsies. RESULTS: Thirty-nine H. pylori positive (mean age 40.5 and 69.2% women) and 24 negative patients (mean age 37.3 and 75% women) were included. The influence of the infection was observed in the duodenal eosinophil count, which was higher in infected individuals: median 13.2 vs 8.1 in non-infected individuals (P=0.005). When we analyzed patients according to symptoms, cases - mean age 39.6; 71.4% women - and controls - mean age 38.7; 71.4% women - had similar duodenal eosinophil count: median 11.9 and 12.6 respectively (P=0.19). CONCLUSIONS: We did not demonstrate association of duodenal eosinophil count with functional dyspepsia but found association with H. pylori infection.

scholarly journals HELICOBACTER PYLORI INFECTION;

2017 ◽  
Vol 24 (09) ◽  
pp. 1425-1430
Author(s):  
Tahira Tabassum ◽  
Ayesha Imtiaz ◽  
Aamir Sharif ◽  
Muhammad Akram
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Helicobacter Pylori Infection ◽  
Step Test ◽  
Chromatographic Technique ◽  
Medical College ◽  
Education Levels ◽  
One Step ◽  
H Pylori

Objectives: To estimate the prevalence of Helicobacter pylori infection inpopulation of Sargodha and determination of possible risk factors. Design: Descriptive study.Place and Duration: University medical complex & Research Center, Sargodha Medical College,Sargodha from 01.01.2017 to 31.03.2017. Material and Methods: The study was conductedamong 486 clinically suspected individuals of Sargodha, Punjab, Pakistan. Data was obtainedby questionnaire and H. pylori antibodies were detected by H. pylori ‘One Step Test Device’based on immune-chromatographic technique. Results: A total of 486 individuals participatedin the study of which 327 (67.28%) were positive for H. pylori infection. The prevalence was66.97% in males and 33.2% in females (p = 0.00) and increased with increasing age (p =0.000). In the study, significant association was found between age groups, education levels,food habits, monthly income with H. pylori infection with p values 0.000, 0.000, 0.001, 0.041respectively while no significant association was seen for source of drinking water (p =0.321).Conclusion: The results of present study showed the prevalence of H. pylori in reported areaand confirmed that it is higher in developing countries. It is concluded that prevalence ofinfection with this bacterium can be minimized by increasing the hygenicity, improving the livingstandards and, of course, by educating the nation.

scholarly journals Immunoblot Analysis of Humoral Immune Response to Helicobacter pylori in Children with and without Duodenal Ulcer

2000 ◽  
Vol 38 (5) ◽  
pp. 1777-1781 ◽  
Author(s):  
Gifone A. Rocha ◽  
Andreia M. R. Oliveira ◽  
Dulciene M. M. Queiroz ◽  
Anfrisina S. T. Carvalho ◽  
Ana M. M. F. Nogueira
Keyword(s):  
Immune Response ◽  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Humoral Immune Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Method ◽  
Predictive Values ◽  
Humoral Immune ◽  
Urease Test ◽  
H Pylori

Several studies have demonstrated that enzyme-linked immunosorbent assay is not a sensitive and specific method to diagnoseHelicobacter pylori infection in children, especially in the younger ones. Since serum immune response can also be determined by immunoblotting and it permits the detection of antibodies to virulence factors such as CagA and VacA, we evaluated the accuracy of a commercial immunoblotting test to diagnose H. pyloriinfection and to assess the humoral immune response to differentH. pylori antigens in 122 children who underwent upper gastrointestinal endoscopy. The presence of H. pylori was determined in antral biopsy specimens by culture, preformed urease test, and histological analysis. H. pylori was identified by microbiological and histopathological methods in 66 children (including all of the 21 who had duodenal ulcer). Antibodies toH. pylori were detected in 63 infected children and in 8 noninfected ones. The sensitivity, specificity, and positive and negative predictive values of the immunoblotting test were 95.5, 85.7, 88.7, and 94.1%, respectively. The number of immunoreactive bands increased with age (P = 0.003), and the bands of 35 kDa (P = 0.013); 89 kDa, the VacA antigen (P = 0.001); and 116 kDa, the CagA antigen (P = 0.00004) were more frequently observed in older children. The frequency of the bands of 89 kDa (P = 0.001) and 116 kDa (P = 0.03) was higher in children with duodenal ulcer than in H. pylori-positive children without the disease. In conclusion, the immunoblotting test appears to be useful for the diagnosis of H. pylori infection in children, even in the younger ones.

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