ABSTRACT
The effects of nisin-induced dnaK expression in Lactococcus lactis were examined, and this expression was shown to improve stress tolerance and lactic acid fermentation efficiency. Using a nisin-inducible expression system, DnaK proteins from L. lactis (DnaK
Lla
) and Escherichia coli (DnaK
Eco
) were produced in L. lactis NZ9000. In comparison to a strain harboring the empty vector pNZ8048 (designated NZ-Vector) and one expressing dnaKLla
(designated NZ-LDnaK), the dnaKEco
-expressing strain, named NZ-EDnaK, exhibited more tolerance to heat stress at 40°C in GM17 liquid medium. The cell viability of NZ-Vector was reduced 4.6-fold after 6 h of heat treatment. However, NZ-EDnaK showed 13.5-fold increased viability under these conditions, with a very low concentration of DnaK
Eco
production. Although the heterologous expression of dnaKEco
did not effect DnaK
Lla
production, heat treatment increased the DnaK
Lla
level 3.5- and 3.6-fold in NZ-Vector and NZ-EDnaK, respectively. Moreover, NZ-EDnaK showed tolerance to multiple stresses, including 3% NaCl, 5% ethanol, and 0.5% lactic acid (pH 5.47). In CMG medium, the lactate yield and the maximum lactate productivity of NZ-EDnaK were higher than the corresponding values for NZ-Vector at 30°C. Interestingly, at 40°C, these values of NZ-EDnaK were not significantly different from the corresponding values for the control strain at 30°C. Lactate dehydrogenase (LDH) activity was also found to be stable at 40°C in the presence of DnaK
Eco
. These findings suggest that the heterologous expression of dnaKEco
enhances the quality control of proteins and enzymes, resulting in improved growth and lactic acid fermentation at high temperature.
pH-Dependent Continuous Lactic Acid Fermentation by Lactococcus lactis IO-1 Using Hydrolyzed Sago Starch.