IMMUNOMODULATORY EFFECTS OF FAR-INFRARED RAY IRRADIATION VIA INCREASING CALMODULIN AND NITRIC OXIDE PRODUCTION IN RAW 264.7 MACROPHAGES

2009 ◽  
Vol 21 (05) ◽  
pp. 317-323 ◽  
Author(s):  
Ting-Kai Leung ◽  
Yung-Sheng Lin ◽  
Yen-Chou Chen ◽  
Huey-Fang Shang ◽  
Yi-Hsuan Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Scientific Evidence ◽  
Far Infrared ◽  
No Production ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Immunomodulatory Effects ◽  
Raw 264.7 Macrophages ◽  
Immunomodulatory Properties ◽  
Far Infrared Ray

Far-infrared ray (FIR) radiation has been shown to be beneficial to human health; however, little scientific evidence of its mechanisms has been provided. In the present study, we investigated the effect of nonthermal-enhanced FIR on the expression of calmodulin (Cam) protein and nitric oxide (NO) production by RAW 264.7 macrophages. Results indicated a significant increase in Cam protein in FIR-treated RAW 264.7 macrophages with or without the addition of lipopolysaccharide (LPS). In addition, the amount of NO was slightly higher but increased significantly in FIR plus LPS-treated RAW 264.7 macrophages. Data of the present study provide the first evidence to indicate the immunomodulatory properties of FIR through increasing Cam protein and NO production in RAW 264.7 macrophages.

scholarly journals Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extracts

2009 ◽  
Vol 2 (4) ◽  
pp. 245-249 ◽  
Author(s):  
Eun-Jin Yang ◽  
Eun-Young Yim ◽  
Gwanpil Song ◽  
Gi-Ok Kim ◽  
Chang-Gu Hyun
Keyword(s):  
Nitric Oxide ◽  
Plant Extracts ◽  
Inhibitory Activity ◽  
Inflammatory Diseases ◽  
No Production ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Raw 264.7 Macrophages ◽  
Oxide Production ◽  
Potent Inhibition

Inhibition of nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages by Jeju plant extractsNitric oxide (NO) produced in large amounts by inducible nitric oxide synthase (iNOS) is known to be responsible for the vasodilation and hypotension observed during septic shock and inflammation. Thus, inhibitors of iNOS may be useful candidates for the treatment of inflammatory diseases accompanied by the overproduction of NO. In this study, we prepared alcoholic extracts of Jeju plants and screened them for their inhibitory activity against NO production in lipopolysaccharide (LPS)-activated macrophages. Among the 260 kinds of plant extract tested, 122 extracts showed potent inhibitory activity towards NO production by more than 25% at a concentration of 100 μg/mL. Plants such asMalus sieboldii, Vaccinium oldhamii, Corylus hallaisanensis, Carpinus laxiflora, Styrax obassia, andSecurinega suffruticosashowed the most potent inhibition (above 70%) at a concentration of 100 μg/mL. The cytotoxic effects of the plant extracts were determined by colorimetric MTT assays and most plant extracts exhibited only moderate cytotoxicity at 100 μg/mL. Therefore, these plants should be considered promising candidates for the further purification of bioactive compounds and would be useful for the treatment of inflammatory diseases accompanying overproduction of NO.

Inhibitory Effect of Trimidox on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Macrophages

2007 ◽  
Vol 104 (3) ◽  
pp. 278-281 ◽  
Author(s):  
Syu-ichi Kanno ◽  
Mai Kakuta ◽  
Yasue Kitajima ◽  
Yuu Osanai ◽  
Kaori Kurauchi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Effect ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Raw 264.7 Macrophages ◽  
Oxide Production

scholarly journals Pomegranate juice down‐regulates nitric oxide production in RAW 264.7 macrophages challenged with lipopolysaccharides

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Jennifer A Powell ◽  
Sachin Mahajan ◽  
Shirin Hooshmand ◽  
Brenda J Smith ◽  
Bahram H Arjmandi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Pomegranate Juice ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Raw 264.7 Macrophages ◽  
Oxide Production

Inhibitory effects of cranberry polyphenol and volatile extracts on nitric oxide production in LPS activated RAW 264.7 macrophages

2019 ◽  
Vol 10 (11) ◽  
pp. 7091-7102 ◽  
Author(s):  
Katie Moore ◽  
Luke Howard ◽  
Cindi Brownmiller ◽  
Inah Gu ◽  
Sun-Ok Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Inhibitory Effects ◽  
Raw 264.7 Macrophages ◽  
Oxide Production ◽  
Health Promoting

Cranberry volatiles have received little attention for health-promoting properties.

Ultra-wideband pulses increase nitric oxide production by RAW 264.7 macrophages incubated in nitrate

2001 ◽  
Vol 23 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Ronald L. Seaman ◽  
Jill E. Parker ◽  
Johnathan L. Kiel ◽  
Satnam P. Mathur ◽  
Teri R. Grubbs ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ultra Wideband ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Raw 264.7 Macrophages ◽  
Oxide Production

scholarly journals New Alkaloids and Anti-inflammatory Constituents from the Leaves of Antidesma ghaesembilla

2017 ◽  
Vol 12 (1) ◽  
pp. 1934578X1701200
Author(s):  
Phan Van Kiem ◽  
Le Canh Viet Cuong ◽  
Do Thi Trang ◽  
Nguyen Xuan Nhiem ◽  
Hoang Le Tuan Anh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Inhibitory Activity ◽  
No Production ◽  
Raw 264.7 ◽  
Chemical Methods ◽  
Raw 264.7 Macrophages ◽  
Chemical Structures ◽  
Bv2 Cells ◽  
Inhibitory Activities ◽  
Physical And Chemical

Using various chromatographic methods, two new alkaloids, antidesoic acids A (1) and B (2) along with fourteen known compounds (3-16) were isolated from the leaves of Antidesma ghaesembilla Gaertn. Their chemical structures were elucidated by physical and chemical methods. All the isolated compounds were evaluated for their inhibitory activity on LPS-stimulated nitric oxide (NO) production in BV2 cells and RAW 264.7 macrophages. Bisflavone 8 significantly inhibited LPS-stimulated NO production in BV2 cells and RAW 264.7 macrophages with IC50 values of 5.4 and 8.0 μM, respectively. Compounds 1–3, 7, 10, 12, 14, and 16 showed moderate inhibitory activities with IC50 values ranging from 11.7 to 77.4 μM.

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