scholarly journals MULTIMODAL NONLINEAR OPTICAL IMAGING OF CELL–MATRIX INTERACTION DURING SPINAL CORD INJURY EX VIVO

2011 ◽  
Vol 23 (03) ◽  
pp. 223-230 ◽  
Author(s):  
Yi-Cheng Huang ◽  
Te-Hsuen Chen ◽  
Wen-Chun Kuo ◽  
Sung-Hao Hsu ◽  
Yi-You Huang ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Ex Vivo ◽  
Second Harmonic ◽  
Two Photon ◽  
Matrix Interaction ◽  
Cell Matrix ◽  
Photon Excitation ◽  
Cord Injury ◽  
Near Future

Neurons within spinal cord injury (SCI) are prevented from regeneration because of scar formation. Chondroitinase ABC (ChABC) was reported to promote functional recovery after spinal cord injury. However, the mechanism and the role of ChABC in the recovery are not clear. In this research, we used second harmonic generation (SHG) and two-photon excitation fluorescence (2PEF) images as probes to observe cell–matrix interaction on fibrosis after SCI followed by ChABC treatment. According to our experimental results, the enzyme ChABC could decrease cystic formation dramatically and consequently allow the spinal cord to regenerate. Using immunohistological analysis, we found that treatment with ChABC at the lesion area resulted in fewer chondroitin sulfate proteoglycans (CSPGs) remaining, longer axonal re-growth, and more new developmental neurons. Furthermore, ChABC 1 U/ml was more effective than 5 U/ml treatment. Using the noninvasive technology, SHG and 2PEF images, we could observe cell–matrix interaction clearly, not only in fixed samples but also in unfixed ex vivo samples. This technology presents a potential for clinical use in the near future.

scholarly journals Injectable hydrogels in stroke and spinal cord injury treatment: A review on hydrogel materials, cell-matrix interaction and glia involvement

2021 ◽  
Author(s):  
Po Hen Lin ◽  
Quanbin Dong ◽  
Sing Yian Chew
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Spinal Cord Injury ◽  
Nervous System ◽  
Injectable Hydrogels ◽  
Matrix Interaction ◽  
Cell Matrix ◽  
Cord Injury ◽  
Injury Treatment

Central nervous system (CNS) pathologies, such as stroke and spinal cord injury, remain debilitating issues due to the inhibitory environment in the CNS. Many research works have focused on combinatorial...

scholarly journals Intravital Assessment of Cells Responses to Conducting Polymer-Coated Carbon Microfibres for Bridging Spinal Cord Injury

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 73
Author(s):  
Bilal El Waly ◽  
Vincent Escarrat ◽  
Jimena Perez-Sanchez ◽  
Jaspreet Kaur ◽  
Florence Pelletier ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Immune Cell ◽  
Two Photon ◽  
Sensory Axons ◽  
Promising Therapeutic Approach ◽  
Cord Injury ◽  
Biochemical Signals ◽  
Coated Carbon ◽  
Striking Effect

The extension of the lesion following spinal cord injury (SCI) poses a major challenge for regenerating axons, which must grow across several centimetres of damaged tissue in the absence of ordered guidance cues. Biofunctionalized electroconducting microfibres (MFs) that provide biochemical signals, as well as electrical and mechanical cues, offer a promising therapeutic approach to help axons overcome this blind journey. We used poly(3,4-ethylenedioxythiophene)-coated carbon MFs functionalized with cell adhesion molecules and growth factors to bridge the spinal cord after a partial unilateral dorsal quadrant lesion (PUDQL) in mice and followed cellular responses by intravital two-photon (2P) imaging through a spinal glass window. Thy1-CFP//LysM-EGFP//CD11c-EYFP triple transgenic reporter animals allowed real time simultaneous monitoring of axons, myeloid cells and microglial cells in the vicinity of the implanted MFs. MF biocompatibility was confirmed by the absence of inflammatory storm after implantation. We found that the sprouting of sensory axons was significantly accelerated by the implantation of functionalized MFs after PUDQL. Their implantation produced better axon alignment compared to random and misrouted axon regeneration that occurred in the absence of MF, with a most striking effect occurring two months after injury. Importantly, we observed differences in the intensity and composition of the innate immune response in comparison to PUDQL-only animals. A significant decrease of immune cell density was found in MF-implanted mice one month after lesion along with a higher ratio of monocyte-derived dendritic cells whose differentiation was accelerated. Therefore, functionalized carbon MFs promote the beneficial immune responses required for neural tissue repair, providing an encouraging strategy for SCI management.

scholarly journals Cell viability in three ex vivo rat models of spinal cord injury

2018 ◽  
Vol 234 (2) ◽  
pp. 244-251 ◽  
Author(s):  
Azim Patar ◽  
Peter Dockery ◽  
Linda Howard ◽  
Siobhan S. McMahon
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Viability ◽  
Ex Vivo ◽  
Rat Models ◽  
Cord Injury

scholarly journals Combination of epidural electrical stimulation with ex vivo triple gene therapy for spinal cord injury: a proof of principle study

2021 ◽  
Vol 16 (3) ◽  
pp. 550 ◽  
Author(s):  
HyunJoon Lee ◽  
RustemRobertovich Islamov ◽  
FilipOlegovich Fadeev ◽  
FaridVagizovich Bashirov ◽  
VaheArshaluysovich Markosyan ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Gene Therapy ◽  
Spinal Cord Injury ◽  
Electrical Stimulation ◽  
Ex Vivo ◽  
Cord Injury ◽  
Proof Of Principle

scholarly journals Molecular and functional characterization of detrusor PDGFRα positive cells in spinal cord injury-induced detrusor overactivity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ken Lee ◽  
Sang O Park ◽  
Pil-Cho Choi ◽  
Seung-Bum Ryoo ◽  
Haeyeong Lee ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Detrusor Overactivity ◽  
Ex Vivo ◽  
Functional Characterization ◽  
Interstitial Cells ◽  
Sk Channels ◽  
Loss Of Function ◽  
Cord Injury

AbstractVolume accommodation occurs via a novel mechanism involving interstitial cells in detrusor muscles. The interstitial cells in the bladder are PDGFRα+, and they restrain the excitability of smooth muscle at low levels and prevents the development of transient contractions (TCs). A common clinical manifestation of spinal cord injury (SCI)-induced bladder dysfunction is detrusor overactivity (DO). Although a myogenic origin of DO after SCI has been suggested, a mechanism for development of SCI-induced DO has not been determined. In this study we hypothesized that SCI-induced DO is related to loss of function in the regulatory mechanism provided by PDGFRα+ cells. Our results showed that transcriptional expression of Pdgfra and Kcnn3 was decreased after SCI. Proteins encoded by these genes also decreased after SCI, and a reduction in PDGFRα+ cell density was also documented. Loss of PDGFRα+ cells was due to apoptosis. TCs in ex vivo bladders during filling increased dramatically after SCI, and this was related to the loss of regulation provided by SK channels, as we observed decreased sensitivity to apamin. These findings show that damage to the mechanism restraining muscle contraction during bladder filling that is provided by PDGFRα+ cells is causative in the development of DO after SCI.

In vivo two-photon imaging reveals a role of progesterone in reducing axonal dieback after spinal cord injury in mice

2017 ◽  
Vol 116 ◽  
pp. 30-37 ◽  
Author(s):  
Zhijie Yang ◽  
Wenguang Xie ◽  
Furong Ju ◽  
Akbar khan ◽  
Shengxiang Zhang
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Two Photon ◽  
Cord Injury ◽  
Photon Imaging ◽  
Two Photon Imaging
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