Continuous Preparation of Low-Molecular-Weight Chitosan by the Combination of Ozone Treatment and Membrane Separation

2012 ◽  
Vol 189 ◽  
pp. 16-20
Author(s):  
Chao Wei ◽  
Jin Tian Liang ◽  
Wu Yue ◽  
Ping Jia Yao

The continuous production of water-soluble low-molecular-weight chitosan (WSLMWC) was successfully achieved with an innovative continuous production system which consisted of ozone generating subsystem, degradation subsystem, and fractionation subsystem. The continuous production of the WSLMWC was performed through three uninterrupted steps: the first, the continuous preparation of the ozone from compressed oxygen, the second, the continuous degradation of chitosan by ozone treatment, and the last, the continuous fractionation of the WSLMWC from the degraded chitosan solution (DCS) by ultrafiltration (UF) membrane. The DCS as the most suitable feed supplied to UF membrane was ascertained. Three different WSLMWC products as useful materials in biomedicine and food were obtained, namely LMWC-3KDa, LMWC-5KDa, and LMWC-10KDa.

2008 ◽  
Vol 108 (3) ◽  
pp. 1082-1087 ◽  
Author(s):  
Wu Yue ◽  
Pingjia Yao ◽  
Yuanan Wei ◽  
Shiqian Li ◽  
Fang Lai ◽  
...  

2004 ◽  
Vol 67 (2) ◽  
pp. 396-398 ◽  
Author(s):  
GUO-JANE TSAI ◽  
SHU-LIN ZHANG ◽  
PEI-LING SHIEH

A water-soluble chitosan hydrolysate with high activity against Escherichia coli was obtained during cellulase digestion of chitosan for 18 h. This 18-h hydrolysate is composed of low-molecular-weight chitosan (LMWC), with a molecular weight of 12.0 kDa, and chitooligosaccharides, which are composed of sugars with a degree of polymerization of 1 to 8. LMWC has a strong activity at 100 ppm against many pathogens and yeast species, including Bacillus cereus, E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica serovar Typhi, and Saccharomyces cerevisiae, while the chitooligosaccharides have much weaker antimicrobial activity than does LMWC. Accordingly, the antimicrobial activity against E. coli in the 18-h hydrolysate proved to come mainly from the presence of LMWC.


2019 ◽  
Vol 121 ◽  
pp. 718-726 ◽  
Author(s):  
Nguyen Cong Minh ◽  
Van Hoa Nguyen ◽  
Simona Schwarz ◽  
Willem F. Stevens ◽  
Trang Si Trung

2013 ◽  
Vol 13 (3) ◽  
Author(s):  
Netty Widyastuti ◽  
Teguh Baruji ◽  
Henky Isnawan ◽  
Priyo Wahyudi ◽  
Donowati Donowati

Beta glucan is a polysaccharide compound, generally not soluble inwater and resistant to acid. Beta glucan is used as an immunomodulator (enhancing the immune system) in mammals is usually a beta-glucan soluble in water, easily absorbed and has a low molecular weight. Several example of beta-glucan such as cellulose (β-1 ,4-glucan), lentinan (β-1 0.6-glucan) and (β-1 ,3-glucan), pleuran (β-1, 6 and β-1 ,3-glucan) are isolated from species of fungi Basidiomycota include mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes).The purpose of thisresearch activity is to obtain beta-glucan compound that can be dissolved in water and in alkali derived from fungi Basidiomycota, i.e, Oyster mushrooms (Pleurotus ostreatus) and shiitake (Lentinus edodes). The result of beta-glucan compared to characterize the resulting beta glucan that is molecular structure . The difference of beta glucan extraction is based on the differences in solubility of beta-glucan. Beta glucan could be water soluble and insoluble water.


Cellulose ◽  
2011 ◽  
Vol 18 (4) ◽  
pp. 929-936 ◽  
Author(s):  
Yukiko Enomoto-Rogers ◽  
Hiroshi Kamitakahara ◽  
Arata Yoshinaga ◽  
Toshiyuki Takano

2021 ◽  
pp. 31-33
Author(s):  
Элана Игоревна Ковалева ◽  
Марина Алексеевна Фролова ◽  
Алексей Иванович Албулов ◽  
Валерий Петрович Варламов ◽  
Анатолий Константинович Елисеев

Хитозан и его производные обладают множеством свойств, которые позволяют применять его в пчеловодстве. Он повышает устойчивость организма пчел к неблагоприятным факторам внешней среды и к возбудителям различных заболеваний. В результате проведенных исследований разработаны технологические параметры получения низкомолекулярного хитозана методом ферментативного гидролиза. Chitosan and its derivatives have many properties that allow them to be used in beekeeping. It increases the resistance of the bee organism to adverse environmental factors and to pathogens of various diseases. As a result of the conducted research, the technological parameters for the production of low-molecular-weight chitosan by enzymatic hydrolysis were developed.


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