PCR Detection of Mycobacterium bovis DNA in Cattle Bloods

For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions speciﬁc for mycobacterium tuberculosis complex are used worldwide. In this study,256 whole blood samples were detected using multiplex PCR.2 of all samples were found to be PCR positive. Homogenous comparison of Mycobacterium bovis specific genes with Mycobacterium bovis AF2122/97 all reached up to 99%.

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Molecular Techniques for Identification of Species of the Mycobacterium tuberculosis Complex: The use of Multiplex PCR and an Adapted HPLC Method for Identification of Mycobacterium bovis and Diagnosis of Bovine Tuberculosis

Understanding Tuberculosis - Global Experiences and Innovative Approaches to the Diagnosis ◽

10.5772/33876 ◽

2012 ◽

Cited By ~ 1

Author(s):

Eduardo Eustquio de Souza Figueiredo ◽

Carlos Adam Conte Jnior ◽

Leone Vincius ◽

Flvia Galindo Silvestre Silva ◽

Rafael Silva ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Multiplex Pcr ◽

Mycobacterium Bovis ◽

Bovine Tuberculosis ◽

Mycobacterium Tuberculosis Complex ◽

Hplc Method ◽

Molecular Techniques ◽

Tuberculosis Complex ◽

Identification Of Species

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Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Brazilian Journal of Microbiology ◽

10.1590/s1517-83822014000300012 ◽

2014 ◽

Vol 45 (3) ◽

pp. 841-843 ◽

Cited By ~ 5

Author(s):

F.L.E. Spositto ◽

P.A.Z. Campanerut ◽

L.D. Ghiraldi ◽

C.Q.F. Leite ◽

M.H. Hirata ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Multiplex Pcr ◽

Mycobacterium Bovis ◽

Mycobacterium Tuberculosis Complex ◽

Tuberculosis Complex

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Analysis of KatG Ser315Thr Mutation in Multidrug Resistant Mycobacterium tuberculosis and SLC11A1 Polymorphism in Multidrug Resistance Tuberculosis in Central Development Region of Nepal Using PCR-RFLP Technique: A Pilot Study

Nepal Journal of Biotechnology ◽

10.3126/njb.v1i1.4169 ◽

1970 ◽

Vol 1 (1) ◽

pp. 14-21 ◽

Cited By ~ 1

Author(s):

Raunak Shrestha ◽

Rubin Narayan Joshi ◽

Kriti Joshi ◽

Bal Hari Poudel ◽

Bhupal Govinda Shrestha

Keyword(s):

Mycobacterium Tuberculosis ◽

Whole Blood ◽

Diagnostic Technique ◽

Molecular Diagnostic ◽

Multi Drug Resistance ◽

Base Line ◽

Blood Samples ◽

Pcr Rflp ◽

Development Region ◽

Whole Blood Samples

Ser315Thr mutations in genes encoding the mycobacteria catalase-peroxidase (KatG) has been associated with the major resistance to isoniazid (INH) in Mycobacterium tuberculosis (MTB). Also G/C polymorphisms in INT4 region of the solute carrier family 11 member 1 gene (SLC11A1) and susceptibility towards tuberculosis (TB) has been demonstrated worldwide. 24 drug resistant MTB culture positive samples and 24 whole?blood samples were collected from different TB patients of Central Development Region of Nepal in 2009. A Polymerase Chain Reaction (PCR) - Restriction Fragment Length Polymorphism (RFLP) assay was carried out in order to investigate Ser315Thr KatG mutation and G/C polymorphism in INT4 region. 4 (16.67%) samples out of 24 MTB culture samples demonstrated the Ser315Thr KatG mutation whereas none of the 24 whole blood samples were found to contain G/C polymorphism in INT4. Though no significant correlation could be found between INT4 polymorphism and TB susceptibility, overall scenario of Nepal cannot be drawn from this data. Molecular diagnostic technique such as PCR-RFLP can be used in a robust scale to carry out base line studies in the TB population of Nepal. Key words: Multi?drug resistance; Tuberculosis; PCR; RFLP Nepal Journal of Biotechnology. Jan. 2011, Vol. 1, No. 1 : 14-21

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In vitro activity of linezolid against Mycobacterium tuberculosis complex, including multidrug-resistant Mycobacterium bovis isolates

International Journal of Antimicrobial Agents ◽

10.1016/j.ijantimicag.2006.02.011 ◽

2006 ◽

Vol 28 (1) ◽

pp. 75-78 ◽

Cited By ~ 30

Author(s):

M. Tato ◽

E. Gómez-G. de la Pedrosa ◽

R. Cantón ◽

I. Gómez-García ◽

J. Fortún ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Mycobacterium Bovis ◽

Mycobacterium Tuberculosis Complex ◽

Multidrug Resistant ◽

Vitro Activity ◽

In Vitro Activity ◽

Tuberculosis Complex

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Genomic analysis of Mycobacterium bovis and other members of the Mycobacterium tuberculosis complex by isoenzyme analysis and pulsed-field gel electrophoresis.

Journal of Clinical Microbiology ◽

10.1128/jcm.34.5.1136-1142.1996 ◽

1996 ◽

Vol 34 (5) ◽

pp. 1136-1142 ◽

Cited By ~ 20

Author(s):

M M Feizabadi ◽

I D Robertson ◽

D V Cousins ◽

D J Hampson

Keyword(s):

Mycobacterium Tuberculosis ◽

Mycobacterium Bovis ◽

Gel Electrophoresis ◽

Mycobacterium Tuberculosis Complex ◽

Genomic Analysis ◽

Pulsed Field Gel Electrophoresis ◽

Tuberculosis Complex ◽

Isoenzyme Analysis ◽

Pulsed Field

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Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments

BMC Infectious Diseases ◽

10.1186/s12879-016-1450-1 ◽

2016 ◽

Vol 16 (1) ◽

Cited By ~ 18

Author(s):

Pallavi Sinha ◽

Anamika Gupta ◽

Pradyot Prakash ◽

Shampa Anupurba ◽

Rajneesh Tripathi ◽

...

Keyword(s):

Mycobacterium Tuberculosis ◽

Multiplex Pcr ◽

Mycobacterium Tuberculosis Complex ◽

Tuberculosis Complex ◽

Encoding Gene ◽

Pcr Targeting

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Typing of Bovine Viral Diarrhea Viruses Directly from Blood of Persistently Infected Cattle by Multiplex PCR

Journal of Clinical Microbiology ◽

10.1128/jcm.37.6.2020-2023.1999 ◽

1999 ◽

Vol 37 (6) ◽

pp. 2020-2023 ◽

Cited By ~ 36

Author(s):

S. A. Gilbert ◽

K. M. Burton ◽

S. E. Prins ◽

D. Deregt

Keyword(s):

Cell Culture ◽

Tissue Culture ◽

Multiplex Pcr ◽

Whole Blood ◽

Rna Extraction ◽

Bovine Viral Diarrhea ◽

Infected Cattle ◽

Blood Samples ◽

Viral Diarrhea ◽

Whole Blood Samples

A nested multiplex PCR was developed for genotyping of bovine viral diarrhea viruses (BVDVs). The assay could detect as little as 3 50% tissue culture infective doses of BVDV per ml and typed 42 out of 42 cell culture isolates. BVDV was also successfully typed, with or without RNA extraction, from all 27 whole-blood samples examined from 22 carriers or probable carriers and 5 experimentally infected cattle.

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