Surface Properties and Cell Response of Bioactive Thermally Grown TiO2 Nanofibers

2014 ◽  
Vol 575 ◽  
pp. 219-222
Author(s):  
A.W. Tan ◽  
Belinda Pingguan-Murphy ◽  
Roslina Ahmad ◽  
Sheikh Akbar
Keyword(s):  
Surface Roughness ◽  
Articular Chondrocytes ◽  
Substrate Surface ◽  
Control Sample ◽  
Degree Of Crystallinity ◽  
Crystallographic Structure ◽  
Cartilage Growth ◽  
Bovine Articular Chondrocytes

Titania nanofiber (TiO2 NFs) arrays were fabricated in situ on a Ti-6Al-4V substrate by an oxidation process. Their surface morphology, crystallographic structure, surface roughness and wettability were characterized, as well as their in vitro interaction with bovine articular chondrocytes at different time points. Results showed that TiO2 NFs possessed greater surface roughness, hydrophilicity and degree of crystallinity. The in vitro cell studies revealed that TiO2 NFs substrate triggers enhanced cell adhesion, proliferation and extracellular matrix (ECM) formation compared to the untreated control sample. These results showed that chondrocytes have an affinity to the nanofibrous substrate surface and thus we suggest that such surfaces are suited to be used as an implant designed for cartilage growth.

Evaluation of Surface Properties and In Vitro Characterization of Surface Modified In Situ TiO2 Nanofibers

2015 ◽  
Vol 656-657 ◽  
pp. 63-67
Author(s):  
Ai Wen Tan ◽  
Belinda Pingguan-Murphy ◽  
Roslina Ahmad ◽  
Sheikh Akbar
Keyword(s):  
Surface Roughness ◽  
Articular Chondrocytes ◽  
Degree Of Crystallinity ◽  
X Ray ◽  
Atomic Force ◽  
Bovine Articular Chondrocytes ◽  
Surface Modified

In situ TiO2 nanofiber arrays have been successfully produced directly on a Ti-6Al-4V substrate by using thermal oxidation under a limited supply of oxygen. Their morphology, elemental composition, crystal structure, surface roughness and surface wettability were characterized by field-emission scanning electron microscope (FESEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffractometer (XRD), atomic force microscope (AFM) and contact angle goniometer, respectively. The results of material characterization studies revealed that TiO2 nanofibers possessed greater surface roughness and wettability, as well as the degree of crystallinity. In vitro characterization have also been evaluated by using bovine articular chondrocytes on the resulting TiO2 nanofibrous surface at different time points. Cell adhesion was observed qualitatively by using FESEM and cell proliferation was determined quantitatively by using AlamarBlue reduction assay. The results showed that the TiO2 nanofibrous substrate triggers enhanced chondrocytes adhesion, proliferation, and production of extracellular matrix (ECM) fibrils compared to untreated substrate. These results suggest that the oxidation process produces a surface structure to which chondrocytes affinity, and thus this surface would has potential use in implants designed for cartilaginous applications.

Effect of Substrate Surface Modification on Biomineralization of Osteoblasts

2006 ◽  
Vol 950 ◽  
Author(s):  
Yizhi Meng ◽  
Xiaolan Ba ◽  
Seo-Young Kwak ◽  
Elaine DiMasi ◽  
Meghan Ruppel ◽  
...  
Keyword(s):  
Laser Scanning ◽  
Bone Cells ◽  
Bone Mineralization ◽  
Substrate Surface ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Tissue Scaffold ◽  
Actin Fiber

ABSTRACTUnderstanding how biomineralization occurs in the extracellular matrix (ECM) of bone cells is crucial to the development of a successfully engineered bone tissue scaffold, and to date there has not been a well-established method for the quantitative examination of bone mineralization in situ. We investigated the mechanical properties of MC3T3-E1 osteoblast-like cells and the crystalline properties of their biomineralized ECM in vitro using shear modulation force microscopy (SMFM), confocal laser scanning microscopy (CLSM), synchrotron X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). The elastic modulus of the mineralizing cells increased at time points corresponding to mineral production, whereas that of the non-mineralizing cells did not vary significantly over time. CLSM showed a restructuring of the F-actin fiber network of mineralizing cells with time, which indicates remodeling activities in the cytoskeleton and was not seen in the non-mineralizing cells. Both XRD and FTIR showed that the mineralizing subclone produced hydroxyapatite in situ and that the non-mineralizing subclone was in fact weakly biomineralizing.

In Vitro Exposure to 0.5% Bupivacaine Is Cytotoxic to Bovine Articular Chondrocytes

2006 ◽  
Vol 22 (7) ◽  
pp. 693-699 ◽  
Author(s):  
Constance R. Chu ◽  
Nicholas J. Izzo ◽  
Nicole E. Papas ◽  
Freddie H. Fu
Keyword(s):  
Articular Chondrocytes ◽  
In Vitro Exposure ◽  
Bovine Articular Chondrocytes

Effects of various prostanoids on th in vitro metabolism of bovine articular chondrocytes

1981 ◽  
Vol 22 (3) ◽  
pp. 499-511 ◽  
Author(s):  
D. Mitrovic ◽  
L. Lippiello ◽  
F. Gruson ◽  
F. Aprile ◽  
H.J. Mankin
Keyword(s):  
Articular Chondrocytes ◽  
In Vitro Metabolism ◽  
Bovine Articular Chondrocytes

Immunohistochemical detection of estrogen receptor α in articular chondrocytes from cows, pigs and humans: in situ and in vitro results

2001 ◽  
Vol 183 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Horst Claassen ◽  
Joachim Hassenpflug ◽  
Michael Schünke ◽  
Walter Sierralta ◽  
Hubert Thole ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Articular Chondrocytes ◽  
Estrogen Receptor Α ◽  
Immunohistochemical Detection

scholarly journals Chemical Composition, In Vitro and In Situ Antimicrobial and Antibiofilm Activities of Syzygium aromaticum (Clove) Essential Oil

Plants ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2185
Author(s):  
Miroslava Kačániová ◽  
Lucia Galovičová ◽  
Petra Borotová ◽  
Veronika Valková ◽  
Hana Ďúranová ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Mass Spectra ◽  
Control Sample ◽  
Antibiofilm Activity ◽  
Syzygium Aromaticum ◽  
Food Preservatives ◽  
The Impact

The essential oil of Syzygium (S.) aromaticum (CEO) is known for its good biological activity. The aim of the research was to evaluate in vitro and in situ antimicrobial and antibiofilm activity of the essential oil produced in Slovakia. The main components of CEO were eugenol 82.4% and (E)-caryophyllene 14.0%. The antimicrobial activity was either weak or very strong with inhibition zones ranging from 4.67 to 15.78 mm in gram-positive and gram-negative bacteria and from 8.22 to 18.56 mm in yeasts and fungi. Among the tested bacteria and fungi, the lowest values of MIC were determined for Staphylococcus (S.) aureus and Penicillium (P.) expansum, respectively. The vapor phase of CEO inhibited the growth of the microscopic filamentous fungi of the genus Penicillium when tested in situ on bread. The strongest effect of mycelia inhibition in a bread model was observed against P. expansum at concentrations of 250 and 500 μL/mL. The best antimicrobial activity of CEO in the carrot model was found against P. chrysosenum. Differences between the mass spectra of Bacillus (B.) subtilis biofilms on the tested surfaces (wood, glass) and the control sample were noted from the seventh day of culture. There were some changes in mass spectra of Stenotrophomonas (S.) maltophilia, which were observed in both experimental groups from the fifth day of culture. These findings confirmed the impact of CEO on the protein structure of older biofilms. The findings indicate that, besides being safe and sensorially attractive, S. aromaticum has antimicrobial activity, which makes it a potential substitute for chemical food preservatives.

scholarly journals EVALUATION OF A HYBRID BIOCOMPOSITE OF HA/HDPE REINFORCED WITH MULTI-WALLED CARBON NANOTUBES (MWCNTs) AS A BONE-SUBSTITUTE MATERIAL

2021 ◽  
Vol 55 (5) ◽  
Author(s):  
Ali A. Al- Allaq ◽  
Jenan S. Kashan ◽  
Mohamed T. El-Wakad ◽  
Ahmed M. Soliman
Keyword(s):  
Carbon Nanotubes ◽  
Bone Substitute ◽  
Ringer Solution ◽  
Degree Of Crystallinity ◽  
Crystallographic Structure ◽  
Hot Press ◽  
Multi Wall Carbon Nanotubes ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

In this investigation, multi-wall carbon nanotubes (MWCNT) with various percentages (0.6%, 1%, 1.4%, 2%) were combined into   ​and High-density polyethylene HDPE (60) wt. % and hydroxyapatite (40) wt. % to form biocomposite using hot-press techniques. The surface topography by AFM images illustrates differences in the roughness of the sample's surface with different adding percentages of MWCNT. The DSC technique exhibits the effect of adding MWCNT in different percentages with the degree of crystallinity, which its effect on mechanical properties for samples. The in vitro bioactivity was investigated by immersion the samples in Ringer's solution as simulated body fluid (SBF) at (0, 3, 6, 9, 12) days (after immersing). The FE-SEM and EDx image explained the apatite layers formation on the sample's surface after 3 days immersed in Ringer solution. Based on XRD Technique, after immersion days in the Ringer solution, the crystallographic structure of hydroxyapatite is formed, forming the monetite.  ​The enhancement of bioactivity has been shown during the incorporation of MWCNT into HA/HDPE composite. These results exhibited excellent indications of biocompatibility properties with the possibility of making promising biomaterials for making bone substitute applications.                                                                                                       

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