The Fe-Core/Carbon-Shell Ultrafine Nanopowders as Platform for Biomolecules Grafting

2014 ◽  
Vol 1040 ◽  
pp. 194-198
Author(s):  
N.S. Surgutskaya ◽  
P.S. Postnikov ◽  
Alexandra G. Pershina ◽  
A.I. Galanov ◽  
Marina E. Trusova ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Large Scale ◽  
Fluorescent Protein ◽  
Carbon Shell ◽  
Shell Nanoparticles ◽  
Powder Surface ◽  
Metal Precursors ◽  
Covalent Grafting ◽  
Large Scale Synthesis ◽  
Green Fluorescent

The Fe-core/carbon-shell nanopowders are excellent platform for covalent grafting of biomolecules. The large-scale synthesis of Fe-core/carbon-shell nanoparticles via electropulse erosion of metal precursors in hydrocarbons was developed. The green fluorescent protein was covalently attached to the powder surface via diazonium functionalization and further carbodiimide activation.

scholarly journals Engineering an efficient and bright split Corynactis californica green fluorescent protein

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hau B. Nguyen ◽  
Thomas C. Terwilliger ◽  
Geoffrey S. Waldo
Keyword(s):  
Green Fluorescent Protein ◽  
Protein Interactions ◽  
Large Scale ◽  
Fluorescent Protein ◽  
Protein Translocation ◽  
Fluorescent Proteins ◽  
Protein Crystallization ◽  
Protein Solubility ◽  
Sea Anemones ◽  
Green Fluorescent

AbstractSplit green fluorescent protein (GFP) has been used in a panoply of cellular biology applications to study protein translocation, monitor protein solubility and aggregation, detect protein–protein interactions, enhance protein crystallization, and even map neuron contacts. Recent work shows the utility of split fluorescent proteins for large scale labeling of proteins in cells using CRISPR, but sets of efficient split fluorescent proteins that do not cross-react are needed for multiplexing experiments. We present a new monomeric split green fluorescent protein (ccGFP) engineered from a tetrameric GFP found in Corynactis californica, a bright red colonial anthozoan similar to sea anemones and scleractinian stony corals. Split ccGFP from C. californica complements up to threefold faster compared to the original Aequorea victoria split GFP and enable multiplexed labeling with existing A. victoria split YFP and CFP.

scholarly journals Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulans

2004 ◽  
Vol 3 (5) ◽  
pp. 1359-1362 ◽  
Author(s):  
Lin Yang ◽  
Leena Ukil ◽  
Aysha Osmani ◽  
Francis Nahm ◽  
Jonathan Davies ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Green Fluorescent Protein ◽  
Aspergillus Nidulans ◽  
Large Scale ◽  
Fluorescent Protein ◽  
Gene Replacement ◽  
Deletion Analysis ◽  
Rapid Production ◽  
Promoter Swapping ◽  
Green Fluorescent

ABSTRACT A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.

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