Cloning, Expression and Characterization of β-Glucosidase from L. Delbrueckii Subsp. Delbrueckii in Escherichia coli
2011 ◽
Vol 301-303
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pp. 347-351
Keyword(s):
Gene encoding β-glucosidase was amplified through PCR by using the genome DNA extracted from L .delbrueckii subsp. delbrueckii as a template. The gene encoding β-glucosidase was inserted into a prokaryotic expression vector pET-28a(+) and expressed in E.coli strain BL21(DE3). The gene encoding β-glucosidase was of 1380bp. The nucleotide sequence of the gene encoding β-glucosidase from L. delbrueckii subsp. delbrueckii showed as high as 97.9% homology comparing with that from L. delbrueckii subsp. bulgaricus indicating that the gene encoding β-glucosidase is highly conservative. The enzyme activity was about 34U/mg and the molecular weight of β-glucosidase is about 51 kDa analyzed by SDS-polyacrylamide gel electrophoresis.
1979 ◽
1998 ◽
Vol 64
(8)
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pp. 3029-3035
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2013 ◽
Vol 421
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pp. 354-358
2014 ◽
Vol 884-885
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pp. 498-502
1979 ◽
2003 ◽
Vol 69
(2)
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pp. 980-986
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2007 ◽
Vol 51
(12)
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pp. 4512-4514
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