Stably Express Spider Flagelliform Silk Protein in Bombyx Mori Cell Line by PiggyBac Transposon-Derived Vector
2011 ◽
Vol 332-334
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pp. 779-782
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Keyword(s):
Silkworm BmN4 cells were transfected with the helper plasmid and the piggyBac vector( piggyBac-FLAG) in which was inserted with the spider flagelliform silk expression cassette. Via antibiotic selection, most cells showed stable DsRed-expression. Immuno blot analysis showed that flagelliform silk protein of spider was expressed stably in BmN4 cells. Circular dichroism spectra indicated the existence of β-turn structure in recombinant spider flagelliform protein. The present results suggested that transgenic silkworm directly secreting functional spider silk protein in cocoon by using piggyBac system is feasible.
Keyword(s):
2016 ◽
Vol 152
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pp. 557-571
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2010 ◽
Vol 107
(32)
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pp. 14059-14063
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2014 ◽
Vol 14
(7)
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pp. 936-942
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2011 ◽
Vol 14
(3)
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pp. B67-B75
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2017 ◽
Vol 27
(36)
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pp. 1701427
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2008 ◽
Vol 47
(24)
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pp. 4592-4594
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2014 ◽
Vol 2014
(1)
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pp. 35-42
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