Implication of Temporal Dynamics of Microbial Abundance and Nutrients to Soil Fertility under Biochar Application – Field Experiments Conducted in a Brown Soil Cultivated with Soybean, North China

A short-term experiment was conducted to investigate responses of microbial community composition and nutrients dynamics to biochar-amended brown soil. We examined the effect of biochar on microbial abundance by plate counting method and microbial community composition changes by DGGE, as well as effect on total and available nutrients N, P and K content. Soil pH measurement was also incorporated into our study. The overall results showed that bacterial abundance increased more than actinomycetes, but fungal abundance decreased slightly in biochar amended soil in later sampling times. In terms of microbial community composition, DGGE analysis for bacteria exhibited some specific lanes in biochar treatments. Bacterial community was more sensitive to sampling time, but fungal community was influenced greatly by biochar addition. In our study, total C content increased significantly, as biochar rate and sampling time preceded. Total N increased slightly in later sampling time, and thus C and N ratio increase was obtained. Total P and K changes were not obvious. Extractable N and pH increased. Microbial utilization was assumed to contribute to extractable P and K decrease in later sampling times. Results suggested that biochar incorporation to brown soil might bring potential benefit to soil fertility from N retention in soil. Microbial turnover may feedback P and K to soil as well in the long term. There may also be an implication of beneficial effect on disease alleviation lead by microbial community imbalance.

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Microbial community composition and abundance after millennia of submarine permafrost warming

10.5194/bg-2019-144 ◽

2019 ◽

Author(s):

Julia Mitzscherling ◽

Fabian Horn ◽

Maria Winterfeld ◽

Linda Mahler ◽

Jens Kallmeyer ◽

...

Keyword(s):

Microbial Community ◽

Community Composition ◽

Pore Water ◽

Negative Correlation ◽

Microbial Community Composition ◽

Microbial Abundance ◽

Bacterial Gene ◽

Gene Abundance ◽

Permafrost Warming

Abstract. Warming of the Arctic led to an increase of permafrost temperatures by about 0.3 °C during the last decade. Permafrost warming is associated with increasing sediment water content, permeability and diffusivity and could on the long-term alter microbial community composition and abundance even before permafrost thaws. We studied the long-term effect (up to 2500 years) of submarine permafrost warming on microbial communities along an onshore-offshore transect on the Siberian Arctic Shelf displaying a natural temperature gradient of more than 10 °C. We analysed the in-situ development of bacterial abundance and community composition through total cell counts (TCC), quantitative PCR of bacterial gene abundance and amplicon sequencing, and correlated the microbial community data with temperature, pore water chemistry and sediment physicochemical parameters. On time-scales of centuries, permafrost warming coincided with an overall decreasing microbial abundance while millennia after warming microbial abundance was similar to cold onshore permafrost and DOC content was least. Based on correlation analysis TCC unlike bacterial gene abundance showed a significant rank-based negative correlation with increasing temperature while both TCC and bacterial gene copy numbers showed a negative correlation with salinity. Bacterial community composition correlated only weakly with temperature but strongly with pore-water stable isotope signatures and depth, while it showed no correlation with salinity. Microbial community composition showed substantial spatial variation and an overall dominance of Actinobacteria, Chloroflexi, Firmicutes, Gemmatimonadetes and Proteobacteria which are amongst the microbial taxa that were found to be active in other frozen permafrost environments as well. We suggest that, millennia after permafrost warming by over 10 °C, microbial community composition and abundance show some indications for proliferation but mainly reflect the sedimentation history and paleo-environment and not a direct effect through warming.

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The influence of environmental factors on microbial community composition in a mountain river

Acta Musei Silesiae Scientiae Naturales ◽

10.2478/cszma-2020-0006 ◽

2020 ◽

Vol 69 (1) ◽

pp. 75-88

Author(s):

Kateřina Sovová ◽

Marek Polášek ◽

Jiří Kroča ◽

Hana Mlejnková

Keyword(s):

Microbial Community ◽

Community Composition ◽

Environmental Variables ◽

Microbial Community Composition ◽

Total Cell ◽

Microbial Abundance ◽

Mountain River ◽

Phylogenetic Groups ◽

Spatial Changes ◽

Temporal And Spatial

Abstract Microbial communities are known to be sensitive indicators for water pollution and biomonitoring assessment. In this study, we aimed at observation of microbial abundance and community composition in the mountain river Morávka. The results showed temporal and spatial changes in total cell abundance (TCA). TCA reached 105–106 cells/ml, increasing values were detected towards the mouth with maxima in summer and autumn months. Out of Eubacteria Betaproteobacteria and Cytophaga-Flavobacterium were found to predominate, while Archaea represented only 4.8 % of TCA. Along the flow three distinct patterns of phylogenetic groups' share were revealed related to increasing pollution. Microbial community composition was found to be most significantly dependent on the elevation and the sampling date. Moreover, environmental variables like O2 saturation, water temperature, trophic potential and pH influenced microbial community as well. Statistical analyses showed significant seasonal (23.5 %) and spatial (4.7 %) changes in microbial community composition.

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Microbial community composition and abundance after millennia of submarine permafrost warming

Biogeosciences ◽

10.5194/bg-16-3941-2019 ◽

2019 ◽

Vol 16 (19) ◽

pp. 3941-3958 ◽

Cited By ~ 1

Author(s):

Julia Mitzscherling ◽

Fabian Horn ◽

Maria Winterfeld ◽

Linda Mahler ◽

Jens Kallmeyer ◽

...

Keyword(s):

Microbial Community ◽

Bacterial Community ◽

Community Composition ◽

Pore Water ◽

Negative Correlation ◽

Microbial Community Composition ◽

Microbial Abundance ◽

Bacterial Gene ◽

Permafrost Warming

Abstract. Warming of the Arctic led to an increase in permafrost temperatures by about 0.3 ∘C during the last decade. Permafrost warming is associated with increasing sediment water content, permeability, and diffusivity and could in the long term alter microbial community composition and abundance even before permafrost thaws. We studied the long-term effect (up to 2500 years) of submarine permafrost warming on microbial communities along an onshore–offshore transect on the Siberian Arctic Shelf displaying a natural temperature gradient of more than 10 ∘C. We analysed the in situ development of bacterial abundance and community composition through total cell counts (TCCs), quantitative PCR of bacterial gene abundance, and amplicon sequencing and correlated the microbial community data with temperature, pore water chemistry, and sediment physicochemical parameters. On timescales of centuries, permafrost warming coincided with an overall decreasing microbial abundance, whereas millennia after warming microbial abundance was similar to cold onshore permafrost. In addition, the dissolved organic carbon content of all cores was lowest in submarine permafrost after millennial-scale warming. Based on correlation analysis, TCC, unlike bacterial gene abundance, showed a significant rank-based negative correlation with increasing temperature, while bacterial gene copy numbers showed a strong negative correlation with salinity. Bacterial community composition correlated only weakly with temperature but strongly with the pore water stable isotopes δ18O and δD, as well as with depth. The bacterial community showed substantial spatial variation and an overall dominance of Actinobacteria, Chloroflexi, Firmicutes, Gemmatimonadetes, and Proteobacteria, which are amongst the microbial taxa that were also found to be active in other frozen permafrost environments. We suggest that, millennia after permafrost warming by over 10 ∘C, microbial community composition and abundance show some indications for proliferation but mainly reflect the sedimentation history and paleoenvironment and not a direct effect through warming.

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Analysis of Microbial Communities in Aged Refuse Based on 16S Sequencing

Sustainability ◽

10.3390/su13084111 ◽

2021 ◽

Vol 13 (8) ◽

pp. 4111

Author(s):

Fen Hou ◽

Junjie Du ◽

Ye Yuan ◽

Xihui Wu ◽

Sai Zhao

Keyword(s):

Microbial Community ◽

Soil Fertility ◽

Community Composition ◽

Microbial Community Composition ◽

Alpha Diversity ◽

Amplicon Sequencing ◽

Undisturbed Soil ◽

16S Sequencing ◽

Bacterial Phyla ◽

Aged Refuse

Aged refuse is widely considered to have certain soil fertility. 16S rRNA amplicon sequencing is used to investigate the microbial community of aged refuse. The aged refuse is found to contain higher soil fertility elements (total nitrogen, total phosphorus, total potassium, etc.) and higher concentrations of heavy metals (Pb, Cd, Zn, and Hg). Taxonomy based on operational taxonomic units (OTUs) shows that Actinobacteria, Proteobacteria, Chloroflexi, Acidobacteria, and Gemmatimonadetes are the main bacterial phyla in the two soils and there is a palpable difference in the microbial community composition between the two groups of samples. The genera Paramaledivibacter, Limnochorda, Marinobacter, Pseudaminobacter, Kocuria, Bdellovibrio, Halomonas, Gillisia, and Membranicola are enriched in the aged refuse. Functional predictive analysis shows that both the control soil and aged refuse have a high abundance of “carbohydrate metabolism” and “amino acid metabolism”, and show differences in the abundance of several metabolism pathways, such as “xenobiotics biodegradation and metabolism” and “lipid metabolism”. Aged refuse and undisturbed soil show significant differences in alpha diversity and microbial community composition. Multiple environmental factors (Hg, TN, Cr, Cd, etc.) significantly impact microorganisms’ abundance (Marinobacter, Halomonas, Blastococcus, etc.). Our study provides valuable knowledge for the ecological restoration of closed landfills.

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Effects of bio-organic fertilizer on soil fertility, microbial community composition, and potato growth

ScienceAsia ◽

10.2306/scienceasia1513-1874.2021.039 ◽

2021 ◽

Vol 47 (3) ◽

pp. 347

Author(s):

Weixi Li ◽

Feiyan Zhang ◽

Guanhui Cui ◽

Yana Wang ◽

Jingguo Yang ◽

...

Keyword(s):

Microbial Community ◽

Soil Fertility ◽

Community Composition ◽

Organic Fertilizer ◽

Microbial Community Composition ◽

Potato Growth

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Parasite-host ecology: the limited impacts of an intimate enemy on host microbiomes

Animal Microbiome ◽

10.1186/s42523-020-00061-5 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Cody S. Clements ◽

Andrew S. Burns ◽

Frank J. Stewart ◽

Mark E. Hay

Keyword(s):

Microbial Community ◽

Microbial Communities ◽

Community Composition ◽

Field Experiments ◽

Microbial Community Composition ◽

Foraging Strategy ◽

Coral Growth ◽

Feeding Strategies ◽

Host Health ◽

Host Ecology

Abstract Background Impacts of biotic stressors, such as consumers, on coral microbiomes have gained attention as corals decline worldwide. Corallivore feeding can alter coral microbiomes in ways that contribute to dysbiosis, but feeding strategies are diverse – complicating generalizations about the nature of consumer impacts on coral microbiomes. Results In field experiments, feeding by Coralliophila violacea, a parasitic snail that suppresses coral growth, altered the microbiome of its host, Porites cylindrica, but these impacts were spatially constrained. Alterations in microbial community composition and variability were largely restricted to snail feeding scars; basal or distal areas ~ 1.5 cm or 6–8 cm away, respectively, were largely unaltered. Feeding scars were enriched in taxa common to stressed corals (e.g. Flavobacteriaceae, Rhodobacteraceae) and depauperate in putative beneficial symbionts (e.g. Endozoicomonadaceae) compared to locations that lacked feeding. Conclusions Previous studies that assessed consumer impacts on coral microbiomes suggested that feeding disrupts microbial communities, potentially leading to dysbiosis, but those studies involved mobile corallivores that move across and among numerous individual hosts. Sedentary parasites like C. violacea that spend long intervals with individual hosts and are dependent on hosts for food and shelter may minimize damage to host microbiomes to assure continued host health and thus exploitation. More mobile consumers that forage across numerous hosts should not experience these constraints. Thus, stability or disruption of microbiomes on attacked corals may vary based on the foraging strategy of coral consumers.

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