Purification, Analysis of Structure of a Polysaccharide From the Fruit of Lycium barbarum L.

2012 ◽  
Vol 550-553 ◽  
pp. 1719-1723
Author(s):  
Min Zhang ◽  
Qian Ma ◽  
Jian Wang
Keyword(s):  
Nucleic Acid ◽  
Periodate Oxidation ◽  
Distilled Water ◽  
Lycium Barbarum ◽  
Smith Degradation ◽  
Ft Ir ◽  
Ultraviolet Spectrophotometer

A polysaccharide, coded as LBP5a, was extracted from the Lycium barbarum L. with distilled water. LBP5a was precipitated repeated by ethanol and purified by DEAE-52, Sephadex G-75. The structure of LBP5a was detected by FT-IR spectrometer, Ultraviolet spectrophotometer, experiments of periodate oxidation and Smith degradation. The results show that LBP5a is testified to be homogeneous polysaccharide, and does not contain nucleic acid or protein. Pyranose with 1→3, 1→4 glycosyl linkages mainly exist in LBP5a.

scholarly journals The copolymeric structure of pig skin dermatan sulphate. Isolation and characterization of l-idurono-sulphate-containing oligosaccharides from copolymeric chains

1974 ◽  
Vol 143 (2) ◽  
pp. 379-389 ◽  
Author(s):  
Lars-Åke Fransson ◽  
Lars Cöster ◽  
Birgitta Havsmark ◽  
Anders Malmström ◽  
Ingrid Sjöberg
Keyword(s):  
Periodate Oxidation ◽  
Exchange Chromatography ◽  
Dermatan Sulphate ◽  
Chondroitinase Abc ◽  
Pig Skin ◽  
Isolation And Characterization ◽  
Smith Degradation ◽  
Iduronic Acid ◽  
Acidic Conditions

Dermatan sulphate was degraded by testicular hyaluronidase and an oversulphated fraction was isolated by ion-exchange chromatography. This preparation, which contained fairly long segments derived from the non-reducing terminal portion of the molecule, was subjected to periodate oxidation under acidic conditions. The oxidized iduronic acid residues were cleaved by reduction-hydrolysis (Smith-degradation) (Fransson & Carlstedt, 1974) or by alkaline elimination. The oligosaccharides so obtained contained both GlcUA (glucuronic acid) and IdUA-SO4 (sulphated iduronic acid) residues. Copolymeric oligosaccharides obtained after alkaline elimination were cleaved by chondroitinase-AC into disaccharide and higher oligosaccharides. Since the corresponding oligosaccharides obtained by Smith-degradation were unaffected by this enzyme, it was concluded that the carbohydrate sequences were GalNAc-(IdUA-GalNAc)n-GlcUA-GalNAc. The iduronic acid-containing sequences were resistant to digestion with chondroitinase-ABC. It was demonstrated that the presence of unsulphated N-acetylgalactosamine residues in these sequences could be responsible for the observed effect. This information was obtained in an indirect way. Chemically desulphated dermatan sulphate was found to be a poor substrate for the chondroitinase-ABC enzyme. Moreover, digestion with chondroitinase-ABC of chondroitinase-AC-degraded dermatan sulphate released periodate-resistant iduronic acid-containing oligosaccharides. It is concluded that copolymeric sequences of the following structure are present in pig skin dermatan sulphate: [Formula: see text] N-acetylgalactosamine moieties surrounding IdUA-SO4 residues are unsulphated to a large extent.

scholarly journals GREEN SYNTHESIS AND SPECTROSCOPIC CHARACTERIZATION OF IRON (III) COMPLEX OF UREA FROM HUMAN URINE AND IRON RUST

2020 ◽  
Vol 45 (5) ◽  
Author(s):  
V.O. Uduah ◽  
J.J. Gongden ◽  
M.L. Kagoro ◽  
K.K. Gurumyen ◽  
Y.N. Lohdip ◽  
...  
Keyword(s):  
Human Urine ◽  
Synthesis Method ◽  
Reaction Medium ◽  
Spectroscopic Characterization ◽  
Waste Materials ◽  
Spectroscopic Techniques ◽  
Distilled Water ◽  
Oxide Composition ◽  
Ft Ir

This work presents a dry synthesis of Iron (III) complex with urea isolated from human urine and Fe (III) obtained from iron rust particles. Iron (III), PI (Purified iron rust), was isolated from iron rust in 10% hydrochloric acid, HCl and distilled water respectively. The complex was synthesized via dry-synthesis method using the melted urea as reaction medium. The isolated Fe (III) was characterized by elemental analysis which was done using XRF Cu-Zn method. The complex was prepared in a 1:4 metal to ligand (M-L) ratio. The stoichiometry of reaction indicate a 1:3 ratio of M-L (Fe-U). The complex was characterized by FT-IR, UV-vis, XRF and XRD spectroscopic techniques. The Fe (III) isolate and Fe-U complex shows percentage yields of 35.7% and ~92% respectively. The elemental and oxide composition of Fe and Fe2O3 (i.e., PI) were 40.387% and 57.753% respectively. The results obtained from the characterization of the iron-urea complex, IUC, indicate FT-IR result as symmetric and asymmetric frequencies with peaks of a combination band of Vs (NH) and Vas (NH), C=O and V (C-N) all stretched, XRD showed the crystal to be amorphous. The elemental and oxide composition of the Fe and Fe2O3 in IUC were 40.007 and 44.201 respectively. The results obtained revealed that useful complexes can be synthesized easily from waste materials, such as urine and iron rust particles, which complement Green chemistry.

THE SEPARATION OF CALF THYMUS NUCLEIC ACID AND HISTONE BY ALCOHOL–SALT PRECIPITATION

1955 ◽  
Vol 33 (1) ◽  
pp. 263-271 ◽  
Author(s):  
L. B. Smillie ◽  
A. M. Marko ◽  
G. C. Butler
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acid ◽  
Aqueous Solutions ◽  
Distilled Water ◽  
Salt Precipitation ◽  
Salt Solutions ◽  
Desoxyribonucleic Acid ◽  
Concentrated Aqueous Solutions ◽  
Calf Thymus ◽  
Quantitative Separation

Extraction of the histone of thymonucleoprotein with alcohol and salt has been studied with varying concentrations of alcohol, sodium chloride, and nucleoprotein and with varying pH and temperature. The addition of 0.5–1.0 volumes of ethanol to a solution of nucleoprotein (approximately 1 mgm. N/ml.) in 3 M sodium chloride has been found to effect an almost quantitative separation of desoxyribonucleic acid and protein. It has proved feasible to prepare concentrated aqueous solutions of the extracted protein by successive dialysis against strong salt solutions at −10 °C. and against distilled water at 5 °C. followed by pervaporation at 5 °C. The properties of the isolated nucleate have indicated a highly polymerized product.

Hydrolytic Degradation of Dental Composites

2014 ◽  
Vol 614 ◽  
pp. 113-117 ◽  
Author(s):  
Codruta Sarosi ◽  
Aurora Antoniac ◽  
Cristina Prejmerean ◽  
Ovidiu Cristian Pastrav ◽  
Dan Patroi ◽  
...  
Keyword(s):  
Fourier Transforms ◽  
Artificial Saliva ◽  
Hydrolytic Degradation ◽  
Alcoholic Solution ◽  
Dental Composites ◽  
Infrared Analysis ◽  
Halogen Lamp ◽  
Distilled Water ◽  
Dental Restorations ◽  
Ft Ir

This study evaluated the hydrolytic degradation of two experimental (C1, C2) and two commercial composite (Tetric EvoCeram and Premise) prepared to be used in dental restorations. Two experimental composites and two commercial composites were undergoing hydrolytic degradation in three different medium: distilled water, artificial saliva and alcoholic solution (50/50). The samples were investigated immediately after polymerisation with halogen lamp Optilux 501 and after 33 days of immersion in all three medium, using Fourier transforms infrared analysis (FTIR). FT-IR spectra of samples from the same composite immersed in distilled water, artificial saliva and alcoholic solution, revealed mostly reducing the intensity of the characteristic peaks of Si-O-Si bond less Tetric composite immersed in artificial saliva, which peak intensity remains unchanged.

A Novel Synthesis of Nano-Fluoride-Substituted Hydroxyapatite Through Dialysis

2006 ◽  
Vol 309-311 ◽  
pp. 577-580 ◽  
Author(s):  
Sheng Min Zhang ◽  
W. Zhou ◽  
Jian Liu ◽  
L. Cheng ◽  
P.P. Chen
Keyword(s):  
Removal Efficiency ◽  
Distilled Water ◽  
Novel Synthesis ◽  
New Synthesis ◽  
Impurity Ions ◽  
Scale Preparation ◽  
Ft Ir ◽  
Promising Application ◽  
20 Nm

A novel synthesis of nano-fluoride-substituted hydroxyapatite (nano-F-HAP) was successfully realized through dialysis process within 4 h, which required much shorter time than the methods reported so far. In new preparation, a dialysis was employed to purify unwanted ions. The removal efficiency of impurity ions in dialysis was evaluated by calculating the concentration of the representative irons NH4 + between the inner slurry and outer distilled water. Results showed that total dialysis efficiency came to 94.5%. The final products were characterized by XRD, HR-TEM, and FT-IR. The XRD and FT-IR results strongly supported substitution of F- for OH-, and the TEM pictures further indicated that the final crystals are about 100nm in length and about 20 nm in diameter without obvious aggregation. Therefore, comparing with traditional purifying processing, such a new synthesis provides a promising application for scale preparation of fine nano-F-HAP crystals.

Combined matrix-isolation FT-IR and ab-initio 6-31++G∗∗ studies on tautomeric properties of nucleic acid bases and simpler model molecules

1997 ◽  
Vol 410-411 ◽  
pp. 397-401
Author(s):  
Linda Houben ◽  
Kristien Schoone ◽  
Johan Smets ◽  
Ludwik Adamowicz ◽  
Guido Maes
Keyword(s):  
Nucleic Acid ◽  
Ab Initio ◽  
Matrix Isolation ◽  
Nucleic Acid Bases ◽  
Ft Ir ◽  
Combined Matrix

scholarly journals Degradation of Nucleic Acid by Allomyces macrogynus during the Production of Zoosporangia and Resistant Sporangia

1980 ◽  
Vol 33 (3) ◽  
pp. 393 ◽  
Author(s):  
Jean Youatt
Keyword(s):  
Nucleic Acid ◽  
Acid Solution ◽  
Glutamic Acid ◽  
Inorganic Phosphate ◽  
Distilled Water ◽  
Allomyces Macrogynus ◽  
Purines And Pyrimidines

Allomyces macrogynus plants were induced to make either zoosporangia in distilled water or resistant sporangia in a solution of glucose and glutamic acid. Analyses during the stages of developmentJ showed that plants of both series degraded nucleic acid, releasing uracil, hypoxanthine and guanin~ to the suspending medium. Plants in distilled water released inorganic phosphate to the medium while those in glucose-glutamic acid solution conserved the phosphate as bound phosphate. A. arbuscula also released the purines and pyrimidines.

Preliminary Investigation of Bone Cement Using Calcium Phosphate Glass

2005 ◽  
Vol 284-286 ◽  
pp. 109-112 ◽  
Author(s):  
Byung Hyun Lee ◽  
Min Chul Kim ◽  
Kyoung Nam Kim ◽  
Kwang Mahn Kim ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Calcium Phosphate ◽  
Phosphate Glass ◽  
Setting Time ◽  
Preliminary Investigation ◽  
Distilled Water ◽  
Liquid Ratio ◽  
Ft Ir ◽  
Kinetics Of Dissolution ◽  
Kinetics Of

The mixed pastes of binary calcium phosphate glass with Ca/P ratio of 0.6 and distilled water were set after about 4 hr, while never set when calcium phosphate glass with Ca/P lower than 0.5. Their compressive strength was ranged from 16.0 to 23.3 MPa. When Na2HPO4 solution was used instead of distilled water as liquid phase, the setting time became drastically much shorter. As the mole concentration of Na2HPO4 solution increased from 0.25 M to 2 M, setting time was shortened to 35 min from almost 3 hr, but compressive strength decreased from 28.8 MPa to 13.2 MPa. At constant mole concentration, as the mass ratio of a powder to liquid ratio increased, setting time was shortened and maximum compressive strength was measured when a powder/liquid ratio was 2.5. However, no crystallized phases were detected either during setting or after complete setting. The XRD , FT-IR and SEM examinations indicated that calcium phosphate glass dissolved and then glass phase precipitated again. We concluded, therefore, that Na2HPO4 just affected the kinetics of dissolution and precipitation of CPG. The mechanism of hardening has yet to be studied.

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