Study on the Conditions of Glucoamylase Production of the Rhizopus oryzae by Solid State Fermentation

2013 ◽  
Vol 709 ◽  
pp. 814-818
Author(s):  
Dan Huang ◽  
Yu Long Li ◽  
Yuan Liang ◽  
Yue Huan Yang
Keyword(s):  
Solid State ◽  
Water Content ◽  
Solid State Fermentation ◽  
Culture Medium ◽  
Rhizopus Oryzae ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Culture Time ◽  
Glucoamylase Activity ◽  
Glucoamylase Production

The conditions of glucoamylase production of theRhizopus oryzaeinLuzhou-flavorDaQu by solid state fermentation were studied. According to the Box-Benhnken design, the conditiones of glucoamylase production of theRhizopus oryzaewere optimized by solid state fermentation. The results showed the optimum conditions of glucoamylase production of theRhizopus oryzaeinLuzhou-flavorDaQu by solid state fermentation were culture temperature 29°C, the water content in culture medium 53%, culture time 164h, glucoamylase activity was 2194.44U/g.

Study on Degradation Performances of Klebsiella Spp. For Cyclohexane

2014 ◽  
Vol 955-959 ◽  
pp. 479-483
Author(s):  
Wen Jie Jin ◽  
Chun Yan Lei ◽  
Ying Liu ◽  
Qi Qi Xing
Keyword(s):  
Petroleum Hydrocarbons ◽  
Theoretical Basis ◽  
Liquid Culture ◽  
Culture Medium ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Initial Concentration ◽  
Culture Time ◽  
Liquid Culture Medium ◽  
Klebsiella Spp

Degradation performances of Klebsiella spp. for Cyclohexane was studied by using the method of single factor in the paper. The influences of temperature, pH, initial concentration of Cyclohexane, oxygen supply, culture time etc. were studied and the optimum conditions were determined. The results show that the activated strains of Klebsiella spp. are inoculated in the liquid culture medium of 100 ml which contained cyclohexane, the initial concentration of cyclohexane is 2490 mg/L, the culture temperature is at 28°C, pH is 6, the number of Klebsiella spp. is about 9×105ml-1in the liquid culture medium, the speed of rotary shaker is 120 r/min, and the degradation rate of Cyclohexane reaches 70.1% in 96 hr. The results could provide the theoretical basis in the field of pollution treatment of petroleum hydrocarbons.

Optimization of glucoamylase production by Mucor indicus, Mucor hiemalis, and Rhizopus oryzae through solid state fermentation / Mucor indicus, Mucor hiemalis, ve Rhizopus oryzae tarafından üretien glukoamilazın katı hal fermantasyonu ile optimizasyonu

2016 ◽  
Vol 41 (4) ◽  
Author(s):  
Sanaz Behnam ◽  
Keikhosro Karimi ◽  
Morteza Khanahmadi ◽  
Zahra Salimian
Keyword(s):  
Solid State ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Rhizopus Oryzae ◽  
Industrial Applications ◽  
Mucor Hiemalis ◽  
Suitable Candidate ◽  
Cultivation Time ◽  
Mucor Indicus ◽  
Glucoamylase Production

AbstractObjective: Glucoamylase is a hydrolyzing enzyme with several industrial applications. Glucoamylase was produced via a solid state fermentation by three naturally occurring zygomycetes fungi of Mucor indicus, Mucor hiemalis, and Rhizopus oryzae on wheat bran.Methods: The effects of cultivation temperature, medium moisture content, and cultivation time on the enzyme production were investigated. Experiments were designed with an orthogonal central composite design on the three variables using response surface methodology (RSM).Results: For glucoamylase production, the optimum temperature and medium moisture content for the three fungi were 26.6°C and 71.8%, respectively. The optimum cultivation time for M. hiemalis and R. oryzae was 33.1 h, while it was 66.8 h for M. indicus. At optimum conditions, glucoamylase production by M. indicus, M. hiemalis, and R. oryzae was respectively 255.3, 272.3, and 1545.3 U per g dry substrate.Conclusion: R. oryzae is a suitable candidate for industrial production of glucoamylase.

Optimization of Process Conditions of Aspergillus oryzae Koji by Response Surface Methodology

2013 ◽  
Vol 798-799 ◽  
pp. 1099-1104
Author(s):  
Ying Ying Ma ◽  
Zhong Kai Zhao ◽  
Qing Bin Liu ◽  
Jian Gang Yang
Keyword(s):  
Response Surface ◽  
Aspergillus Oryzae ◽  
Process Condition ◽  
Process Conditions ◽  
Single Factor ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Design Expert ◽  
Culture Time ◽  
Glucoamylase Activity

Effects of inoculation quantity, culture temperature and time on glucoamylase activity and the optimum conditions of koji-making of Aspergillus oryzae was studied by using single factor and response surface experiment. The results show that 3 factors can significantly impact on glucoamylase activity of koji. The best process condition of koji-making obtained by using the "Design-Expert" software is: culture temperature 38.70 °C, inoculation quantity 0.85 , and culture time 50.47 h.

scholarly journals Solid-state fermentation process for gibberellin production using enzymatic hydrolysate corn stalks

BioResources ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 429-443
Author(s):  
Jing Bai ◽  
Fengli Liu ◽  
Siqi Li ◽  
Pan Li ◽  
Chun Chang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Fermentation Process ◽  
Culture Medium ◽  
Culture Period ◽  
Mass Ratio ◽  
Optimum Conditions ◽  
Enzymatic Hydrolysate ◽  
Gibberellin Production ◽  
Fermentation Period

Solid-state fermentation was carried out for production of gibberellin via the addition of enzymatic hydrolysate from steam-exploded corn stalks during the culture period. The enzymatic hydrolysate from the steam-exploded corn stalks was added to the culture medium during the solid-state fermentation period, which improved gibberellin production. When the enzymatic hydrolysate was added into the 400 mL/kg dry basis substrate in the solid-state fermentation after 60 h, the temperature was 30 °C, the pH was 7.00, the mass ratio of solid to liquid was 1:1.1, and the fermentation period was 168 h. This led to the largest gibberellin yield (9.48 g/kg dry basis), and when compared with pre-optimization, the gibberellin yield increased by 135%. The optimum conditions to maximize the biomass for the fermentation process were obtained; the temperature was 32 °C for a gibberellin yield of 9.20 g/kg dry basis, the pH was 6.00 and the mass ratio of solid to liquid was 1:1.1 for a gibberellin yield of 9.48 g/kg dry basis, and the fermentation period was 96 h for a gibberellin yield of 6.94 g/kg dry basis. Therefore, a new alternative way for gibberellin production via solid-state fermentation has been demonstrated.

scholarly journals PRODUCTION AND CHARACTERIZATION OF CELLULOLYTIC ENZYMES BY ASPERGILLUS NIGER AND RHIZOPUS SP. BY SOLID STATE FERMENTATION OF PRICKLY PEAR

2016 ◽  
Vol 29 (1) ◽  
pp. 222-233 ◽  
Author(s):  
TAMIRES CARVALHO DOS SANTOS ◽  
GEORGE ABREU FILHO ◽  
AILA RIANY DE BRITO ◽  
AURELIANO JOSÉ VIEIRA PIRES ◽  
RENATA CRISTINA FERREIRA BONOMO ◽  
...  
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Water Activity ◽  
Solid State Fermentation ◽  
Cellulase Production ◽  
Cellulolytic Enzymes ◽  
Optimum Conditions ◽  
Thermostable Enzymes ◽  
Fermentation Time ◽  
Rhizopus Sp

ABSTRACT: Prickly palm cactus husk was used as a solid-state fermentation support substrate for the production of cellulolytic enzymes using Aspergillus niger and Rhizopus sp. A Box-Behnken design was used to evaluate the effects of water activity, fermentation time and temperature on endoglucanase and total cellulase production. Response Surface Methodology showed that optimum conditions for endoglucanase production were achieved at after 70.35 h of fermentation at 29.56°C and a water activity of 0.875 for Aspergillus niger and after 68.12 h at 30.41°C for Rhizopus sp. Optimum conditions for total cellulase production were achieved after 74.27 h of fermentation at 31.22°C for Aspergillus niger and after 72.48 h and 27.86°C for Rhizopus sp. Water activity had a significant effect on Aspergillus niger endoglucanase production only. In industrial applications, enzymatic characterization is important for optimizing variables such as temperature and pH. In this study we showed that endoglucanase and total cellulase had a high level of thermostability and pH stability in all the enzymatic extracts. Enzymatic deactivation kinetic experiments indicated that the enzymes remained active after the freezing of the crude extract. Based on the results, bioconversion of cactus is an excellent alternative for the production of thermostable enzymes.

scholarly journals Cow Dung Substrate for the Potential Production of Alkaline Proteases by Pseudomonas putida Strain AT in Solid-State Fermentation

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Ponnuswamy Vijayaraghavan ◽  
Sreekumar Saranya ◽  
Samuel Gnana Prakash Vincent
Keyword(s):  
Solid State ◽  
Pseudomonas Putida ◽  
Solid State Fermentation ◽  
Maximum Activity ◽  
Protease Production ◽  
Cow Dung ◽  
Alkaline Proteases ◽  
Optimum Conditions ◽  
Potential Production ◽  
Fermentation Period

Cow dung and agroresidues were used as the substrates for the production of alkaline proteases by Pseudomonas putida strain AT in solid-state fermentation. Among the various substrates evaluated, cow dung supported maximum (1351±217 U/g) protease production. The optimum conditions for the production of alkaline proteases were a fermentation period of 48 h, 120% (v/w) moisture, pH 9, and the addition of 6% (v/w) inoculum, 1.5% (w/w) trehalose, and 2.0% (w/w) yeast extract to the cow dung substrate. The enzyme was active over a range of temperatures (50–70°C) and pHs (8–10), with maximum activity at 60°C and pH 9. These enzymes showed stability towards surfactants, detergents, and solvent and digested various natural proteins.

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