Optimization of Process Conditions of Aspergillus oryzae Koji by Response Surface Methodology

2013 ◽  
Vol 798-799 ◽  
pp. 1099-1104
Author(s):  
Ying Ying Ma ◽  
Zhong Kai Zhao ◽  
Qing Bin Liu ◽  
Jian Gang Yang
Keyword(s):  
Response Surface ◽  
Aspergillus Oryzae ◽  
Process Condition ◽  
Process Conditions ◽  
Single Factor ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Design Expert ◽  
Culture Time ◽  
Glucoamylase Activity

Effects of inoculation quantity, culture temperature and time on glucoamylase activity and the optimum conditions of koji-making of Aspergillus oryzae was studied by using single factor and response surface experiment. The results show that 3 factors can significantly impact on glucoamylase activity of koji. The best process condition of koji-making obtained by using the "Design-Expert" software is: culture temperature 38.70 °C, inoculation quantity 0.85 , and culture time 50.47 h.

Study on the Conditions of Glucoamylase Production of the Rhizopus oryzae by Solid State Fermentation

2013 ◽  
Vol 709 ◽  
pp. 814-818
Author(s):  
Dan Huang ◽  
Yu Long Li ◽  
Yuan Liang ◽  
Yue Huan Yang
Keyword(s):  
Solid State ◽  
Water Content ◽  
Solid State Fermentation ◽  
Culture Medium ◽  
Rhizopus Oryzae ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Culture Time ◽  
Glucoamylase Activity ◽  
Glucoamylase Production

The conditions of glucoamylase production of theRhizopus oryzaeinLuzhou-flavorDaQu by solid state fermentation were studied. According to the Box-Benhnken design, the conditiones of glucoamylase production of theRhizopus oryzaewere optimized by solid state fermentation. The results showed the optimum conditions of glucoamylase production of theRhizopus oryzaeinLuzhou-flavorDaQu by solid state fermentation were culture temperature 29°C, the water content in culture medium 53%, culture time 164h, glucoamylase activity was 2194.44U/g.

scholarly journals Homogenate Extraction of Crocins from Saffron Optimized by Response Surface Methodology

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yingpeng Tong ◽  
Yu Jiang ◽  
Dan Guo ◽  
Yongqiu Yan ◽  
Shiping Jiang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Process Conditions ◽  
Extraction Temperature ◽  
Single Factor ◽  
Extraction Voltage ◽  
Homogenate Extraction

Saffron, which has many kinds of biological activities, has been widely used in medicine, cosmetics, food, and other fields of health promotion industries. Crocins are the main component of saffron (Crocus sativus L.). At present, most of the extraction methods for crocins require long time or special instruments to complete the process and some of them are not suitable for industrial production at present. In this article, homogenate extraction technology which is a convenient and efficient method was developed for crocins extraction from saffron. Firstly, the influences of extraction voltage, extraction time, ethanol concentration, and temperature on crocins yield were studied by single factor experiments; and then response surface methodology (RSM) was used to optimize levels of four variables based on the result of single factor experiments. Results showed that the optimum extraction process conditions for crocins were as follows: extraction voltage, 110 V; ethanol concentration, 70%; extraction temperature, 57°C; and extraction time, 40 s. Based on these conditions, the extraction yield of crocins can reach 22.76% which is higher than ultrasonic extraction method. Therefore, homogenate extraction is an effective way to extract crocins from saffron with higher extraction yield and shorter extraction time.

Optimization of Microwave Drying of CuCl Residue Using Response Surface Methodology

2013 ◽  
Vol 803 ◽  
pp. 3-8 ◽  
Author(s):  
Yong Gang Zuo ◽  
Li Bo Zhang ◽  
Bing Guo Liu ◽  
Jin Hui Peng ◽  
Ai Yuan Ma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Microwave Drying ◽  
Process Conditions ◽  
Optimum Conditions ◽  
Drying Temperature ◽  
Microwave Roasting ◽  
Dehydration Rate ◽  
Material Thickness ◽  
Rsm Technique

Abstract: The technology that CuCl residue from Zn hydrometallurgy was dried by microwave heating was studied. The influence of the drying duration, drying temperature and material thickness on dehydration rate was investigated. The response surface methodology (RSM) technique was utilized to optimize the process conditions. The optimum conditions for drying CuCl residue have been identified to be an drying temperature of 80°C, drying duration of 11 min and material thickness of 16 mm. The optimum conditions resulted in an CuCl residue with moisture content of 4.97%, which could ensure remove chlorine of CuCl residue by microwave roasting.

scholarly journals Optimization of Prebiotics and Oxygen Scavengers for Bifidobacterium bifidum BB01 Microcapsules by Response Surface Methodology

2018 ◽  
Vol 22 (1) ◽  
pp. 3-12
Author(s):  
Yilin Li ◽  
Guowei Shu ◽  
Yichao Li ◽  
Yu Liu ◽  
Yajuan Song
Keyword(s):  
Ascorbic Acid ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Screening Test ◽  
Xanthan Gum ◽  
Bifidobacterium Bifidum ◽  
Single Factor ◽  
Optimum Conditions ◽  
Oxygen Scavengers ◽  
Single Factor Experiment

Abstract The effects of different prebiotics and oxygen scavengers on making the process of xanthan gum and chitosan (XC) Bifidobacterium bifidum BB01 microcapsules were studied by single factor experiment and Plackett-Burman screening test, ascorbic acid, sodium erythorbate and xylo-oligosaccharide had significant effects. Based on the previous studies, the process XC B. Bifidum BB01 microcapsules were further optimized by Box-Behnken model in this study. Response surface analysis showed that the best additive amoumt of ascorbic acid, sodium erythorbate and xylo-oligosaccharide were 3.0%, 2.36% and 4.99%, respectively. The viable counts of B. Bifidum BB01 microcapsules reached to 1.52×1010CFU/g from 1.25×1010 CFU/g, the encapsulation yield reached to 94.88% from 90% under the optimum conditions. It provided the research foundation for the afterward production and exploration of the process XC B. Bifidum BB01 microcapsules.

Study on Degradation Performances of Klebsiella Spp. For Cyclohexane

2014 ◽  
Vol 955-959 ◽  
pp. 479-483
Author(s):  
Wen Jie Jin ◽  
Chun Yan Lei ◽  
Ying Liu ◽  
Qi Qi Xing
Keyword(s):  
Petroleum Hydrocarbons ◽  
Theoretical Basis ◽  
Liquid Culture ◽  
Culture Medium ◽  
Optimum Conditions ◽  
Culture Temperature ◽  
Initial Concentration ◽  
Culture Time ◽  
Liquid Culture Medium ◽  
Klebsiella Spp

Degradation performances of Klebsiella spp. for Cyclohexane was studied by using the method of single factor in the paper. The influences of temperature, pH, initial concentration of Cyclohexane, oxygen supply, culture time etc. were studied and the optimum conditions were determined. The results show that the activated strains of Klebsiella spp. are inoculated in the liquid culture medium of 100 ml which contained cyclohexane, the initial concentration of cyclohexane is 2490 mg/L, the culture temperature is at 28°C, pH is 6, the number of Klebsiella spp. is about 9×105ml-1in the liquid culture medium, the speed of rotary shaker is 120 r/min, and the degradation rate of Cyclohexane reaches 70.1% in 96 hr. The results could provide the theoretical basis in the field of pollution treatment of petroleum hydrocarbons.

Primary Study on Optimization of the Processing Technology in Aspergillus oryzae Seed Koji by Homogeneous Design

2013 ◽  
Vol 798-799 ◽  
pp. 1071-1074
Author(s):  
Ying Ying Ma ◽  
Zhong Kai Zhang ◽  
Xun Hai Pan ◽  
Jian Gang Yang
Keyword(s):  
Aspergillus Oryzae ◽  
Incubation Time ◽  
Culture Conditions ◽  
Wood Ash ◽  
Processing Technology ◽  
Primary Study ◽  
Culture Temperature ◽  
Culture Time ◽  
Spore Number

The homogeneous was used to optimize the processing technology of Aspergillus oryzae seed koji. The results show that the culture time is the most significant factor and the sequence of the residual factors are the square of culture time, the interaction between temperature and the time, the interaction of time and wood ash addition, the square of wood ash addition and the inoculation quantity. The results reveal that the optimized culture conditions are proved to be that the incubation time is 9 days, the inoculation quantity is 0.1×107/g, the dosage of wood ash is 1.267% and the culture temperature is 40°C. Under this condition, the prospective spore number of seed koji has reached to 1.89×109 /g.

Extraction of Chondroitin Sulfate from Tilapia Byproducts with Ultrasonic-Microwave Synergistic

2013 ◽  
Vol 726-731 ◽  
pp. 4381-4385 ◽  
Author(s):  
Chang Yu Cheng ◽  
Wei Wen Duan ◽  
Zhen Hua Duan ◽  
Yang Hai ◽  
Xin Gui Lei ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Chondroitin Sulfate ◽  
Response Surface ◽  
Microwave Power ◽  
Oreochromis Niloticus ◽  
Optimum Process ◽  
Ultrasonic Power ◽  
Single Factor ◽  
Optimum Conditions ◽  
Extraction And Separation

The optimum conditions for the extraction and separation of chondroitin sulfate from tilapia (Oreochromis niloticus) byproducts were studied in the paper. The chondroitin sulfate was extracted by the combination of ultrasonic and microwave. On the basis of the single factor tests, the response surface methodology was taken to study the optimum process of extraction. Results show that the best conditions for the extraction and separation of chondroitin sulfate from tilapia byproducts are: the ultrasonic power is 50W, the microwave power is 98W, the extraction time is 120s, the ratio of solid to liquid is 1:50 (g/mL), and under these conditions, the extraction rate of chondroitin sulfate is 2.513%.

scholarly journals Optimization of Extraction Process of Methyl Eugenol and Asarinin in Asarum with Deep Eutectic Solvent Based on the Response Surface Methodology

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Huiwei Bao ◽  
Yang Xu ◽  
Yuejie Wang ◽  
Tao Zhang ◽  
Enpeng Wang ◽  
...  
Keyword(s):  
Response Surface ◽  
Deep Eutectic Solvent ◽  
Extraction Process ◽  
Methyl Eugenol ◽  
Extraction Rate ◽  
Process Conditions ◽  
Surface Model ◽  
Single Factor ◽  
Extraction Technology ◽  
Ultrasonic Time

To explore a green and efficient extraction technology for the extraction of active ingredients of Asarum, the deep eutectic solvent combined with ultrasonic was applied to compare the extraction efficiency of 10 kinds of deep eutectic solvents, taking the extraction rate of methyl eugenol and asarinin as indices. Single-factor experiments were adopted to investigate the influence of molar ratio, liquid-to-solid ratio, eddy time, ultrasonic time, and temperature of the deep eutectic solvent on the extraction rate of methyl eugenol and asarinin. Based on single-factor experiments, the surface response methodology was used to optimize the extraction process conditions. The results showed that the optimum extracting process conditions of methyl eugenol and asarinin in Asarum consisted of a ratio of choline chloride to glycerol of 1 : 3, a DES volume of 2 mL, an ultrasonic temperature of 60°C, an ultrasonic time of 30 min, and a vortex oscillation of 7 min. Under the optimum extracting process conditions, the contents of methyl eugenol and asarinin were 1.9428 mg/g and 0.9989 mg/g, respectively, and the comprehensive index was 2.3280 (RSD of 1.91%). The results were close to the predicted values of the response surface model, demonstrating the applicability of the model. The extraction rate of methyl eugenol and asarinin in Asarum by this method was higher than that of water extraction and alcohol extraction, which fully indicated the high efficiency of ultrasonic-assisted green deep eutectic solvent extraction technology. The results provide data support for further development and utilization of Asarum.

scholarly journals Improvement the Resveratrol Content of Germinated Peanut Drink by Lactic Acid Fermentation

2021 ◽  
pp. 262-273
Author(s):  
SongLok Uh ◽  
IlJin Kim ◽  
KiBong Kim ◽  
YongIl Seo ◽  
KilNam Shin ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Response Surface ◽  
Raw Materials ◽  
Chemical Constituents ◽  
Single Factor ◽  
Optimum Conditions ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Single Factor Analysis ◽  
Prevention Of Cardiovascular Disease

Because peanut is a legume of nutrient abundance and contains a wide variety of chemical constituents such as proteins, carbohydrates, fibers, fats, niacin, folate, thiamine, resveratrol, flavonoids, magnesium, and phosphorus, a lot of researcher focus the study on the peanut. Especially the peanut has high content of resveratrol, so the health benefits including anti-aging, anticancer, anti-inflammatory and the prevention of cardiovascular disease, therefore the study that the peanut is used to process food and treat disease carried out widely. In this study, the condition to optimize the process programmes of fermentative germinated peanut drink by response surface experiment and to increase resveratrol contents by lactic acid bacteria is determined. In order to improve the resvertrol contents of fermentative germinated peanut drink, was prepared by using four-day germinated peanut as raw materials?adding Lactobacillus and xylitol before pasteurized, fermentation and cold storge. By single factor analysis and response surface experiments, the optimum conditions for fermentative germinated peanut drink were the amount of inoculum 3.26%, the amount of xylitol 6.2%, the fermentation time 15h and the ratio of material to water 1:5(g/mL). Product quality was evaluated through sensory evaluation.  Investigate the change in resveratrol content of fermentative germinated peanut drink by HPLC. Resveratrol contents were increased from 674.22 ±2.47 μg/L to 815.82±4.53 μg/L in germination peanut drink after fermentation.

Dyeing Property of Shikonin on Wool Fabric

2011 ◽  
Vol 233-235 ◽  
pp. 1306-1309
Author(s):  
Li Hua Lv ◽  
Chun Yan Wei ◽  
Fang Ye ◽  
Huan Zhang
Keyword(s):  
Factor Analysis ◽  
Wool Fabric ◽  
Process Conditions ◽  
Single Factor ◽  
Optimum Conditions ◽  
Dyeing Property ◽  
Specific Process ◽  
Wool Fabrics ◽  
Liquor Ratio ◽  
Single Factor Analysis

In this paper, wool fabric was dyed by Shikonin. The optimum conditions of dyeing wool fabric with Shikonin were obtained by the orthogonal and single factor analysis experiments. The specific process conditions are as follows: liquor ratio 1:40; dye concentration 80ml/L; pH 4; temperature 80; time 60min. The results indicated that fastness to washing and to rubbing of wool fabrics dyed with Shikonin was good and has reached more than three level.

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