Isolation and Genetic Characterization of Phenol-Degradating Bacterium from a Salt Lake in Inner Mongolia

2013 ◽  
Vol 726-731 ◽  
pp. 396-400
Author(s):  
Gang Dai ◽  
La Mei Liu ◽  
Qing Chao Ouyang ◽  
Chao Luo Meng ◽  
Tat Suo Iwasa
Keyword(s):  
Inner Mongolia ◽  
Genetic Characterization ◽  
Salt Lake ◽  
Phenol Hydroxylase ◽  
Ring Cleavage ◽  
Lake Ecosystems ◽  
The World ◽  
Dioxygenase Gene ◽  
Multicomponent Phenol Hydroxylase

Salt lake environments are economically and ecologically important issues. Like any other ecosystems in the world, salt lake ecosystems are facing various kinds of pollutions. However, little information is available on the biodegradation of organic pollutants by halophilic microorganisms in such environments. In the present work we isolated a strain of phenol-degradating bacteria from a salt lake in Inner Mongolia. Sequence of 16S rRNA suggested that the strain belong toPropionibacteriumgenus. The strain can grow with phenol as a sole carbon source under 10% (w/v) salt concentration. PCR amplifications with the primers for multicomponent phenol hydroxylase or catechol 1, 2 dioxygenase gene were applied to genome DNA of the strain, suggesting that phenol might be transformed to catechol by multicomponent phenol hydroxylase and further degradated through ortho-ring cleavage pathway. When the primers for catechol 1, 2 dioxygenase were applied to the DNA obtained from the lake water, at least two types of catechol 1, 2 dioxygenase genes sequences were obtained, suggesting the presence of the bacteria with diverse aromatic compound-degradating genes.

scholarly journals Characterization of the Gallate Dioxygenase Gene: Three Distinct Ring Cleavage Dioxygenases Are Involved in Syringate Degradation by Sphingomonas paucimobilis SYK-6

2005 ◽  
Vol 187 (15) ◽  
pp. 5067-5074 ◽  
Author(s):  
Daisuke Kasai ◽  
Eiji Masai ◽  
Keisuke Miyauchi ◽  
Yoshihiro Katayama ◽  
Masao Fukuda
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequences ◽  
Terminal Region ◽  
Ring Cleavage ◽  
Sphingomonas Paucimobilis ◽  
Acid Protein ◽  
Demethylation Pathway ◽  
Dioxygenase Gene ◽  
Amino Acid Protein

ABSTRACT Sphingomonas paucimobilis SYK-6 converts vanillate and syringate to protocatechuate (PCA) and 3-O-methylgallate (3MGA) in reactions with the tetrahydrofolate-dependent O-demethylases LigM and DesA, respectively. PCA is further degraded via the PCA 4,5-cleavage pathway, whereas 3MGA is metabolized via three distinct pathways in which PCA 4,5-dioxygenase (LigAB), 3MGA 3,4-dioxygenase (DesZ), and 3MGA O-demethylase (LigM) are involved. In the 3MGA O-demethylation pathway, LigM converts 3MGA to gallate, and the resulting gallate appears to be degraded by a dioxygenase other than LigAB or DesZ. Here, we isolated the gallate dioxygenase gene, desB, which encodes a 418-amino-acid protein with a molecular mass of 46,843 Da. The amino acid sequences of the N-terminal region (residues 1 to 285) and the C-terminal region (residues 286 to 418) of DesB exhibited ca. 40% and 27% identity with the sequences of the PCA 4,5-dioxygenase β and α subunits, respectively. DesB produced in Escherichia coli was purified and was estimated to be a homodimer (86 kDa). DesB specifically attacked gallate to generate 4-oxalomesaconate as the reaction product. The Km for gallate and the V max were determined to be 66.9 ± 9.3 μM and 42.7 ± 2.4 U/mg, respectively. On the basis of the analysis of various SYK-6 mutants lacking the genes involved in syringate degradation, we concluded that (i) all of the three-ring cleavage dioxygenases are involved in syringate catabolism, (ii) the pathway involving LigM and DesB plays an especially important role in the growth of SYK-6 on syringate, and (iii) DesB and LigAB are involved in gallate degradation.

scholarly journals Genetic Characterization of Animal Brucella Isolates from Northwest Region in China

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Xiaoan Cao ◽  
Youjun Shang ◽  
Yongsheng Liu ◽  
Zhaocai Li ◽  
Zhizhong Jing
Keyword(s):  
Inner Mongolia ◽  
Genetic Characterization ◽  
Agar Medium ◽  
Northwest China ◽  
Northwest Region ◽  
Multilocus Sequencing

Animal brucellosis is a reemerging disease in China, particular in northwest China. The Brucella species (even genus) are highly conserved; therefore the use of Multilocus sequencing typing (MLST: based on conserved housekeeping loci) is more suitable for discrimination at species or biovar level on Brucella. In this study, MLST was used to analyze the characterization of Brucella from sheep and yaks during 2015 and 2016. All 66 isolates were collected from northwest China, including Inner Mongolia, Xinjiang, Qinghai, and Gansu provinces. Isolates were cultured on Brucella agar medium and identified by MLST. MLST identified five ST types: ST8 (n=55), ST7 (n=2), ST3 (n=5), ST1 (n=2), and ST14 (n=2). This analysis revealed that B. melitensis isolates exhibited high single genotypes (ST8) in the most northwest China. MLST of isolates provides helpful information on understanding genetic characterization of Brucella in northwest China.

scholarly journals Genetic characterization of three mitochondrial gene sequences of goat/sheep-derived coenurus cerebralis and cysticercus tenuicollis isolates in Inner Mongolia, China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 1 ◽  
Author(s):  
Yichi Zhang ◽  
Wei Zhao ◽  
Di Yang ◽  
Yuan Tian ◽  
Weizhe Zhang ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Genetic Characterization ◽  
Mitochondrial Gene ◽  
Northern China ◽  
Nucleotide Sequences ◽  
Taenia Hydatigena ◽  
Cysticercus Tenuicollis ◽  
Coenurus Cerebralis ◽  
Epidemiological Significance

Taenia multiceps and Taenia hydatigena are widely distributed tapeworms of canids. Due to a lack of genetic information on these two parasites in China, in this study we analyzed six coenurus cerebralis and two cysticercus tenuicollis cysts from goats or sheep in Inner Mongolia, northern China by amplifying three mitochondrial genes (cox1, nad4, and cytb). Two haplotypes were obtained at each locus for either of the two Taenia cestode species, with ten nucleotide sequences being novel. The degrees of genetic variations were 1.18%, 0.61% and 0.52% for coenurus cerebralis, and 0.24%, 0.46% and 0.35% for cysticercus tenuicollis at the cox1, nad4 and cytb loci, respectively. This is the first molecular description of animal-derived metacestodes of T. multiceps and T. hydatigena in Inner Mongolia, China. Novel nucleotide sequences might reflect endemic genetic characterization of the two cestodes. The present data are useful to explore the biological and epidemiological significance of intra-specific variations within both Taenia cestodes.

scholarly journals Shedding of Vaccine Viruses with Increased Antigenic and Genetic Divergence after Vaccination of Newborns with Monovalent Type 1 Oral Poliovirus Vaccine

2009 ◽  
Vol 83 (17) ◽  
pp. 8693-8704 ◽  
Author(s):  
Sabine van der Sanden ◽  
Mark A. Pallansch ◽  
Jan van de Kassteele ◽  
Nasr El-Sayed ◽  
Roland W. Sutter ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Oral Poliovirus Vaccine ◽  
World Health ◽  
Study Group ◽  
Poliovirus Type ◽  
The World ◽  
Birth Dose ◽  
Health Organization

ABSTRACT For the final stages in the eradication of poliovirus type 1 (P1), the World Health Organization advocates the selective use of monovalent type 1 oral poliovirus vaccine (mOPV1). To compare the immunogenicity of mOPV1 with that of trivalent OPV (tOPV) in infants, a study was performed in Egypt in 2005. Newborns were vaccinated with mOPV1 or tOPV immediately after birth and were challenged with mOPV1 after 1 month. Vaccination with mOPV1 at birth resulted in significantly higher seroconversion against P1 viruses and lower excretion of P1 viruses than vaccination with tOPV. Intratypic differentiation of the viruses shed by the newborns revealed the presence of remarkably high numbers of antigenically divergent (AD) P1 isolates, especially in the mOPV1 study group. The majority of these AD P1 isolates (71%) were mOPV1 challenge derived and were shed by newborns who did not seroconvert to P1 after the birth dose. Genetic characterization of the viruses revealed that amino acid 60 of the VP3 region was mutated in all AD P1 isolates. Isolates with substitution of residue 99 of the VP1 region had significantly higher numbers of nonsynonymous mutations in the VP1 region than isolates without this substitution and were preferentially shed in the mOPV1 study group. The widespread use of mOPV1 has proven to be a powerful tool for fighting poliovirus circulation in the remaining areas of endemicity. This study provides another justification for the need to achieve high vaccination coverage in order to prevent the circulation of AD strains.

Characterization of an Intradiol Ring-Cleavage Dioxygenase Gene Associated With Abamectin Resistance in Tetranychus cinnabarinus (Acari: Tetranychidae)

2019 ◽  
Vol 112 (4) ◽  
pp. 1858-1865
Author(s):  
Zhifeng Xu ◽  
Peilin Liu ◽  
Yuan Hu ◽  
Jia Hu ◽  
Cuicui Qi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Spider Mite ◽  
Gene Sequence ◽  
Ring Cleavage ◽  
Tetranychus Cinnabarinus ◽  
Limited Data ◽  
Dioxygenase Gene ◽  
Carmine Spider Mite ◽  
Rnai Technique

AbstractTetranychus cinnabarinus (Boisduval), i.e., carmine spider mite, is a worldwide pest that can cause serious damage to plants. Problems of resistance have arisen since abamectin usage in the control of T. cinnabarinus. Unfortunately, there are only limited data on the extent of this problem. To understand the development of abamectin resistance in the carmine spider mite, we prokaryotically expressed an intradiol ring-cleavage dioxygenase (ID-RCD) gene sequence, TcID-RCD1, which had a significant upregulated expression of over 7.7 times in an abamectin-resistant strain (AbR) when compared with that of a susceptible strain (SS). The crude enzyme activity also indicated that the AbR had a higher activity than that exhibited in SS. When susceptible individuals were treated with abamectin, TcID-RCD1 was also overexpressed. Furthermore, using the RNA interference (RNAi) technique, TcID-RCD1 was successfully knocked down, with the expression level decreasing significantly to approximately 39% in the SS strain compared with the control. And the mortality of mites feeding on dsTcID-RCD1 increased significantly when treated with abamectin. These results strongly suggest that TcID-RCD1 is involved in abamectin resistance in T. cinnabarinus.

Genetic characterization of Wolbachia from Great Salt Lake brine flies

Symbiosis ◽  
2016 ◽  
Vol 72 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Amanda Truong ◽  
Mohammad Sondossi ◽  
Jonathan B. Clark
Keyword(s):  
Genetic Characterization ◽  
Salt Lake ◽  
Great Salt Lake ◽  
Salt Lake Brine

Histopathological and genetic characterization of colorectal mucinous carcinoma

2001 ◽  
Vol 120 (5) ◽  
pp. A166-A166
Author(s):  
S FUJII ◽  
T KUSAKA ◽  
T KAIHARA ◽  
Y UEDA ◽  
T CHIBA ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mucinous Carcinoma

Genetic characterization of high hyperdiploidy in childhood acute lymphoblastic leukemia

2009 ◽  
Vol 221 (03) ◽  
Author(s):  
R Vagkopoulou ◽  
C Eckert ◽  
U Ungethüm ◽  
G Körner ◽  
M Stanulla ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Genetic Characterization ◽  
Lymphoblastic Leukemia ◽  
Childhood Acute Lymphoblastic Leukemia

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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