Study on Enzymatic Synthesis of γ-Cyclodextrin with β-Cyclodextrin

2013 ◽  
Vol 774-776 ◽  
pp. 901-906
Author(s):  
Xin Zhi Cao ◽  
Jian Ming You ◽  
Kai Bin Lv
Keyword(s):  
Reaction Time ◽  
Enzymatic Synthesis ◽  
Cyclodextrin Glucanotransferase ◽  
Beta Cyclodextrin ◽  
Gamma Cyclodextrin

Gamma cyclodextrin was produced synthesized from beta cyclodextrin in the presence of glycyrrhizin, using Cyclodextrin glucanotransferase (EC 2.4.1.19) from AlkalophilicBacillus. The results showed that more than 32.7% of beta cyclodextrin substrate was converted to the corresponding gamma cyclodextrin under the following conditions, viz. 200 units enzyme/g of β-CD, 10mmol/L of β-CD concentration, 3% of glycyrrhizin concentration, 27 h of reaction time, 65°C of temperature and a reaction pH of 7.0.

scholarly journals Enzymatic Synthesis and Anti-Allergic Activities of Curcumin Oligosaccharides

2010 ◽  
Vol 3 ◽  
pp. BCI.S2768 ◽  
Author(s):  
Kei Shimoda ◽  
Hiroki Hamada
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Enzymatic Synthesis ◽  
Antibody Formation ◽  
Cyclodextrin Glucanotransferase ◽  
Enzymatic Method ◽  
Inhibitory Effects ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Suppressive Action

Curcumin 4‘- O-glucooligosaccharides were synthesized by a two step-enzymatic method using almond β-glucosidase and cyclodextrin glucanotransferase (CGTase). Curcumin was glucosylated to curcumin 4‘- O-β-D-glucopyranoside by almond β-glucosidase in 19% yield. Curcumin 4‘- O-β-D-glucopyranoside was converted into curcumin 4‘- O-β-glucooligosaccharides, i.e. 4‘- O-β-maltoside (51%) and 4‘- O-β-maltotrioside (25%), by further CGTase-catalyzed glycosylation. Curcumin 4‘- O-β-glycosides showed suppressive action on IgE antibody formation and inhibitory effects on histamine release from rat peritoneal mast cells.

scholarly journals Optimization of Enzymatic Synthesis of Betulinic Acid Amide in Organic Solvent by Response Surface Methodology (RSM)

2019 ◽  
Vol 19 (4) ◽  
pp. 849
Author(s):  
Nurul Atikah Amin Yusof ◽  
Nursyamsyila Mat Hadzir ◽  
Siti Efliza Ashari ◽  
Nor Suhaila Mohamad Hanapi ◽  
Rossuriati Dol Hamid
Keyword(s):  
Response Surface Methodology ◽  
Reaction Time ◽  
Response Surface ◽  
Reaction Temperature ◽  
Betulinic Acid ◽  
Enzymatic Synthesis ◽  
Acid Amide ◽  
Molar Ratio ◽  
Percentage Yield ◽  
Substrate Molar Ratio

Optimization of the lipase catalyzed enzymatic synthesis of betulinic acid amide in the presence of immobilized lipase, Novozym 435 from Candida antartica as a biocatalyst was studied. Response surface methodology (RSM) and 5-level-4-factor central-composite rotatable design (CCRD) were employed to evaluate the effects of the synthesis parameters, such as reaction time (20–36 h), reaction temperature (37–45 °C), substrate molar ratio of betulinic acid to butylamine (1:1–1:3), and enzyme amounts (80–120 mg) on the percentage yield of betulinic acid amide by direct amidation reaction. The optimum conditions for synthesis were: reaction time of 28 h 33 min, reaction temperature of 42.92 °C, substrate molar ratio of 1:2.21, and enzyme amount of 97.77 mg. The percentage yield of actual experimental values obtained 65.09% which compared well with the maximum predicted value of 67.23%. The obtained amide was characterized by GC, GCMS and 13C NMR. Betulinic acid amide (BAA) showed a better cytotoxicity compared to betulinic acid as the concentration inhibited 50% of the cell growth (IC50) against MDA-MB-231 cell line (IC50 < 30 µg/mL).

scholarly journals Enzymatic Synthesis of a Novel Pterostilbene α-Glucoside by the Combination of Cyclodextrin Glucanotransferase and Amyloglucosidase

Molecules ◽  
2018 ◽  
Vol 23 (6) ◽  
pp. 1271 ◽  
Author(s):  
José González-Alfonso ◽  
David Rodrigo-Frutos ◽  
Efres Belmonte-Reche ◽  
Pablo Peñalver ◽  
Ana Poveda ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Cyclodextrin Glucanotransferase

scholarly journals Synthesis of Capsaicin Glycosides and 8-Nordihydrocapsaicin Glycosides as Potential Weight-Loss Formulations

2010 ◽  
Vol 3 ◽  
pp. BCI.S2676 ◽  
Author(s):  
Hisashi Katsuragi ◽  
Kei Shimoda ◽  
Eriko Kimura ◽  
Hiroki Hamada
Keyword(s):  
Weight Loss ◽  
Body Weight ◽  
High Fat Diet ◽  
Enzymatic Synthesis ◽  
Cyclodextrin Glucanotransferase ◽  
Inhibitory Effects ◽  
High Fat ◽  
Potential Weight

The enzymatic synthesis of capsaicin glycosides and 8-nordihydrocapsaicin glycosides was investigated using almond β-glucosidase and cyclodextrin glucanotransferase (CGTase). Capsaicin and 8-nordihydrocapsaicin were converted into their β-glucoside and β-maltooligosaccharide (amylose conjugate), i.e. β-maltoside and β-maltotrioside, by sequencial glycosylation with almond β-glucosidase and CGTase. The β-glucoside and β-maltoside of capsaicin and β-glucoside of 8-nordihydrocapsaicin showed inhibitory effects on high-fat-diet-induced elevations in body weight of mice.

Enzymatic synthesis of piceid glycosides by cyclodextrin glucanotransferase

2012 ◽  
Vol 47 (3) ◽  
pp. 528-532 ◽  
Author(s):  
Sindhu Mathew ◽  
Martin Hedström ◽  
Patrick Adlercreutz
Keyword(s):  
Enzymatic Synthesis ◽  
Cyclodextrin Glucanotransferase
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