Human Metallothionein Enhanced Tolerance and Biosorption of Cadmium when Expressed in Saccharomyces cerevisiae

2013 ◽  
Vol 779-780 ◽  
pp. 195-200 ◽  
Author(s):  
Bo Liang ◽  
Min Li ◽  
Jie Shang
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Adsorption Capacity ◽  
Recombinant Strain ◽  
Chemical Method ◽  
Gene Sequence ◽  
Recombinant Strains ◽  
Codon Preference ◽  
Cadmium Compounds ◽  
Hepatic Metallothionein ◽  
Rapid Adsorption

The recombinant Saccharomyces cerevisiae expressing human hepatic metallothionein (MT) was constructed for biosorption of cadmium (II). The gene sequence of mt was modified for codon preference of S. cerevisiae and synthesized using chemical method. The maximal biosorption capability of cadmium compounds Cd2+ of the recombinant increased more than 25.8% compared with the control. For MT-expressed recombinant strains, a rapid adsorption occurred within the first 30 min with a significant level of Cd2+ (55.75mg/g). For comparison, S. cerevisiae cells reach its maximal Cd2+ adsorption capacity (45.02 mg/g) until 2h. Furthermore, recombinant strain were able to withstand the toxicity of Cd2+ and grow. The results indicated that recombinant should be useful in enhancement the tolerance and biosorption of cadmium in practice.

scholarly journals Synergistic effect of carboxyl and sulfate groups for effective removal of radioactive strontium ion in a Zr-metal-organic framework

2021 ◽  
Author(s):  
Lin Ren ◽  
Xudong Zhao ◽  
Baosheng Liu ◽  
Hongliang Huang
Keyword(s):  
Adsorption Capacity ◽  
High Efficiency ◽  
Metal Organic Framework ◽  
Maximum Adsorption Capacity ◽  
Potential Candidate ◽  
Dual Groups ◽  
Metal Organic ◽  
Strontium Ion ◽  
Rapid Adsorption ◽  
Organic Framework

Abstract Rapid removal of radioactive strontium from nuclear wastewater is of great significance for environment safety and human health. This work reported the effective adsorption of strontium ion in a stable dual-group metal-organic framework, Zr6(OH)14(BDC-(COOH)2)4(SO4)0.75 (Zr-BDC-COOH-SO4), which contains strontium-chelating groups (-COOH and SO4) and strongly ionizable group (-COOH). Zr-BDC-COOH-SO4 exhibits very rapid adsorption kinetics (<5 min) and a maximum adsorption capacity of 67.5 mg g−1. The adsorption behaviors can be well evaluated by pseudo-second-order model and Langmuir isotherm model. Further investigations indicate that the adsorption of Sr2+ in Zr-BDC-COOH-SO4 would not be interfered by solution pH and adsorption temperature obviously. Feasible regeneration of the adsorbent was also demonstrated through a simple elution method. Mechanism investigation suggests that free -COOH contributes to the rapid adsorption based on electrostatic interaction while introduction of -SO4 can enhance the adsorption capacity largely. Thus, these results suggest that Zr-BDC-COOH-SO4 might be a potential candidate for Sr2+ removal and introducing dual groups is an effective strategy for designing high-efficiency adsorbents.

Bioethanol production performance of five recombinant strains of laboratory and industrial xylose-fermenting Saccharomyces cerevisiae

2009 ◽  
Vol 100 (8) ◽  
pp. 2392-2398 ◽  
Author(s):  
Akinori Matsushika ◽  
Hiroyuki Inoue ◽  
Katsuji Murakami ◽  
Osamu Takimura ◽  
Shigeki Sawayama
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Production Performance ◽  
Bioethanol Production ◽  
Recombinant Strains

Adsorption of Reactive Red 120 by Using Regenerated Spent Carbon

2015 ◽  
Vol 754-755 ◽  
pp. 671-675
Author(s):  
Muhammad Ridwan Fahmi ◽  
M.N. Nasruddin ◽  
Che Zulzikrami Azner Abidin ◽  
M. Ali Umi Fazara
Keyword(s):  
Adsorption Capacity ◽  
Azo Dye ◽  
Chemical Method ◽  
Dye Removal ◽  
Thermal Method ◽  
Column Test ◽  
Pyrolysis Process ◽  
Continuous Adsorption ◽  
Reactive Red 120 ◽  
Common Application

In this study the performance of regenerated spent carbon for azo dye removal was evaluated in batch and continuous column. The spent carbon was generated by thermal method through pyrolysis process and chemical method by using NaOH 6.0 M solution. Reactive Red 120 (RR120) was selected as a model of azo dye due to its common application in the industries. The regeneration of spent granular activated carbon (GAC) by pyrolysis could produce adsorbent that has the adsorption capacity closer to new GAC. The result indicated that paralysed GAC could potentially apply to replace new GAC for RR120 adsorption. In addition, the continuous adsorption operation in mini column test confirmed that the order of adsorption capacity of each GAC is as follows: new GAC, pirolysed GAC, chemical treated GAC and spent GAC.

scholarly journals SUHU DAN WAKTU OPTIMUM PROSES EKSTRAKSI ANTOSIANIN DALAM UBI JALAR UNGU (Ipomoea batatas L.) DENGAN ?-L-ARABINOFURANOSIDASE

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 88
Author(s):  
I N. Wirajana ◽  
N. M. T. Juliasari ◽  
A.A. I.A.M. Laksmiwati ◽  
N. W. Bogoriani
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sweet Potato ◽  
Recombinant Strain ◽  
Ipomoea Batatas ◽  
Optimum Temperature ◽  
Assisted Extraction ◽  
Purple Sweet Potato ◽  
Time Required ◽  
Enzyme Application ◽  
Enzyme Assisted Extraction

Enzyme-assisted extraction (EAE) method is one of the most environmentally friendly methods of enzyme application in the extraction of bioactive compounds. The purpose of this study was to determine the optimum temperature and time required in the extraction of anthocyanin compounds from purple sweet potato (Ipomoea batatas L.) with and without ?-L-arabinofuranosidase (AbfA) - assisted. The AbfA enzyme was obtained from Saccharomyces cerevisiae recombinant strain BJ1824 contain pYHMI-Af plasmid. The optimum temperature and time in the extraction of anthocyanin compound with and without  ?-L-arabinofuranosidase  from purple sweet potato were performed on the 40, 50, 60 and 700C; and 150, 180, 210 minutes. The extraction was done by ethanol solvent of 60,32% (v/v) acidified with citric acid of 2,39% (b/v). The measurement of anthocyanin levels using UV-Vis Spectrophotometer at 527 nm and 700 nm wavelengths at pH 1,0 and 4,5. The optimum condition of non-enzyme-assisted extraction was at 600C for 210 minutes, with the anthocyanin levels of 26,3842 mg/L; while with the AbfA enzyme-assisted at 500C for 180 minutes, with the anthocyanin levels of 28,2056 mg/L. The extraction with enzyme-assisted resulted the anthocyanin levels of 6,90% higher than without the using of enzyme.

Recombinant strains of Saccharomyces cerevisiae for ethanol production from plant biomass

2015 ◽  
Vol 5 (4) ◽  
pp. 375-382
Author(s):  
A. S. Rozanov ◽  
A. V. Kotenko ◽  
I. R. Akberdin ◽  
S. E. Peltek
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Plant Biomass ◽  
Recombinant Strains

scholarly journals Investigation of Two Distinct Flavone Synthases for Plant-Specific Flavone Biosynthesis in Saccharomyces cerevisiae

2005 ◽  
Vol 71 (12) ◽  
pp. 8241-8248 ◽  
Author(s):  
Effendi Leonard ◽  
Yajun Yan ◽  
Kok Hong Lim ◽  
Mattheos A. G. Koffas
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Strain ◽  
Plant Secondary Metabolites ◽  
Dependent Manner ◽  
Recombinant Yeast Strain ◽  
Membrane Bound ◽  
Flavone Synthase ◽  
Flavone Synthase Ii ◽  
Dose Dependent

ABSTRACT Flavones are plant secondary metabolites that have wide pharmaceutical and nutraceutical applications. We previously constructed a recombinant flavanone pathway by expressing in Saccharomyces cerevisiae a four-step recombinant pathway that consists of cinnamate-4 hydroxylase, 4-coumaroyl:coenzyme A ligase, chalcone synthase, and chalcone isomerase. In the present work, the biosynthesis of flavones by two distinct flavone synthases was evaluated by introducing a soluble flavone synthase I (FSI) and a membrane-bound flavone synthase II (FSII) into the flavanone-producing recombinant yeast strain. The resulting recombinant strains were able to convert various phenylpropanoid acid precursors into the flavone molecules chrysin, apigenin, and luteolin, and the intermediate flavanones pinocembrin, naringenin, and eriodictyol accumulated in the medium. Improvement of flavone biosynthesis was achieved by overexpressing the yeast P450 reductase CPR1 in the FSII-expressing recombinant strain and by using acetate rather than glucose or raffinose as the carbon source. Overall, the FSI-expressing recombinant strain produced 50% more apigenin and six times less naringenin than the FSII-expressing recombinant strain when p-coumaric acid was used as a precursor phenylpropanoid acid. Further experiments indicated that unlike luteolin, the 5,7,4′-trihydroxyflavone apigenin inhibits flavanone biosynthesis in vivo in a nonlinear, dose-dependent manner.

scholarly journals Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport inSaccharomyces cerevisiae

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Chengqiang Wang ◽  
Yu Shen ◽  
Yanyan Zhang ◽  
Fan Suo ◽  
Jin Hou ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Strain ◽  
Consumption Rate ◽  
Specific Growth ◽  
Ethanol Yield ◽  
Maximum Specific Growth Rate ◽  
Dry Cell Weight ◽  
Utilization Pathway ◽  
Dry Cell ◽  
Arabinose Fermentation

The L-arabinose utilization pathway was established inSaccharomyces cerevisiae, by expressing the codon-optimizedaraA,araB, andaraDgenes ofLactobacillus plantarum. After overexpressing theTAL1,TKL1,RPE1,RKI1, andGAL2genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h−1, a maximum specific L-arabinose consumption rate of 0.61 g h−1 g−1dry cell weight, and a promising ethanol yield of 0.43 g g−1from L-arabinose fermentation.

scholarly journals Micronuclear DNA of Oxytricha nova contains sequences with autonomously replicating activity in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (9) ◽  
pp. 1725-1729 ◽  
Author(s):  
M M Colombo ◽  
M T Swanton ◽  
P Donini ◽  
D M Prescott
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Sequence ◽  
Yeast Cells ◽  
Dna Molecules ◽  
Autonomously Replicating Sequence ◽  
Restriction Fragments ◽  
Nuclear Rna ◽  
Oxytricha Nova

Oxytricha nova is a hypotrichous ciliate with micronuclei and macronuclei. Micronuclei, which contain large, chromosomal-sized DNA, are genetically inert but undergo meiosis and exchange during cell mating. Macronuclei, which contain only small, gene-sized DNA molecules, provide all of the nuclear RNA needed to run the cell. After cell mating the macronucleus is derived from a micronucleus, a derivation that includes excision of the genes from chromosomes and elimination of the remaining DNA. The eliminated DNA includes all of the repetitious sequences and approximately 95% of the unique sequences. We cloned large restriction fragments from the micronucleus that confer replication ability on a replication-deficient plasmid in Saccharomyces cerevisiae. Sequences that confer replication ability are called autonomously replicating sequences. The frequency and effectiveness of autonomously replicating sequences in micronuclear DNA are similar to those reported for DNAs of other organisms introduced into yeast cells. Of the 12 micronuclear fragments with autonomously replicating sequence activity, 9 also showed homology to macronuclear DNA, indicating that they contain a macronuclear gene sequence. We conclude from this that autonomously replicating sequence activity is nonrandomly distributed throughout micronuclear DNA and is preferentially associated with those regions of micronuclear DNA that contain genes.

Sign in / Sign up

Export Citation Format

Share Document