Protective Effect of Ginsenoside Rb1 Mixing with Several Lung Flush Solutions on Pulmonary Ischemia-Reperfusion Injury

2014 ◽  
Vol 884-885 ◽  
pp. 625-629
Author(s):  
Feng Wu Lin ◽  
Chuan Zhang ◽  
Qiang Zhang ◽  
Kun Peng Cheng ◽  
Nan Gao ◽  
...  
Keyword(s):  
Protective Effect ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Weight Ratio ◽  
Protective Effects ◽  
Ginsenoside Rb1 ◽  
Wet Weight ◽  
Pulmonary Ischemia ◽  
Dextran Solution

Objective: To evaluate the protective effects of ginsenoside Rb1 mixed with LPD compared to mixed with several other lung flush solutions on ischemia-reperfusion injury. Method: Three group of rabbit lungs were perfused with three kinds of mixtures of ginsenoside Rb1 with blood, Euro-Collins solution(EC) or low-potassium-dextran solution(LPD) respectively, then lung dry/wet weight ratio and malondiadehyde(MAD) were examined and histological changes were observed. Result: Lung dry/wet weight ratio of LPD and Rb1 group was higher than that of EC and Rb1 and blood and Rb1 groups, whereas MAD of LPD and Rb1 group was significantly less than that of EC and Rb1 and blood and Rb1 groups（P<0.05）. Histological findings showed less damage in LPD and Rb1 group. Conclusion: Ginsenoside Rb1 mixed with LPD shows a better protective effect on pulmonary ischemia-reperfusion injury compared with other mixtures.

scholarly journals Bilobalide protects against ischemia/reperfusion-induced oxidative stress and inflammatory responses via the MAPK/NF-κB pathways in rats

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Ying Li ◽  
Jiliang Jiang ◽  
Liangcheng Tong ◽  
Tingting Gao ◽  
Lei Bai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Muscle Tissue ◽  
Inflammatory Responses ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Weight Ratio ◽  
Dry Weight ◽  
Protective Effects

Abstract Background Clinically, skeletal muscle ischemia/reperfusion injury is a life-threatening syndrome that is often caused by skeletal muscle damage and is characterized by oxidative stress and inflammatory responses. Bilobalide has been found to have antioxidative and anti-inflammatory effects. However, it is unclear whether bilobalide can protect skeletal muscle from ischemia/reperfusion injury. Methods The effects of bilobalide on ischemia/reperfusion-injured skeletal muscle were investigated by performing hematoxylin and eosin staining and assessing the wet weight/dry weight ratio of muscle tissue. Then, we measured lipid peroxidation, antioxidant activity and inflammatory cytokine levels. Moreover, Western blotting was conducted to examine the protein levels of MAPK/NF-κB pathway members. Results Bilobalide treatment could protected hind limb skeletal muscle from ischemia/reperfusion injury by alleviating oxidative stress and inflammatory responses via the MAPK/NF-κB pathways. Conclusions Bilobalide may be a promising drug for I/R-injured muscle tissue. However, the specific mechanisms for the protective effects still need further study.

scholarly journals Dexmedetomidine alleviates pulmonary ischemia-reperfusion injury through modulating the miR-21-5p/Nr4a1 signaling pathway

2020 ◽  
Author(s):  
Wei Dong ◽  
Hongxia Yang ◽  
Minghua Cheng ◽  
Xin Zhang ◽  
Jingjing Yin ◽  
...  
Keyword(s):  
Western Blot ◽  
Cell Viability ◽  
Reperfusion Injury ◽  
Signaling Pathway ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Weight Ratio ◽  
Dry Weight ◽  
Pulmonary Injury ◽  
Pulmonary Ischemia

This study aims to investigate the protection of dexmedetomidine (Dex) against pulmonary ischemia-reperfusion injury (PIRI) in the mouse model and reveal the mechanism in hypoxia reoxygenation (H/R)-induced mouse pulmonary vascular endothelial cells (MPVECs). The lung wet-to-dry weight ratio, histopathological features, and malondialdehyde (MDA) concentrations were measured. The H/R-induced MPVECs were exposed to Dex, and the cell viability, cell apoptosis and protein expressions were assessed by the Cell Counting Kit-8 (CCK8) assay, flow cytometry and western blot, respectively. In addition, the regulatory relationship between miR-21-5p and orphan nuclear receptor 4A1 (Nr4a1) was revealed by several assays, including the dual-luciferase reporter assay, real-time quantitative polymerase chain reaction (RT-qPCR) and western blot. We found that the Dex treatment significantly alleviated pulmonary injury and decreased the level of MDA and wet/dry weight ratio in PIRI mice. Dex treatment also increased cell viability, reduced apoptotic ratio and downregulated expression levels of Cleaved Caspase-3 and Cleaved Caspase-9 in H/R induced MPVECs. Furthermore, the expression of miR-21-5p was upregulated, while Nr4a1 was downregulated by Dex in a concentration-dependent manner in H/R induced MPVECs. Moreover, Nr4a1 was verified as a target of miR-497-5p. Overexpression of Nr4a1 could reverse the protective effects of Dex on alleviating H/R-induced injury in MPVECs. Taken together, Dex treatment attenuated ischemia-reperfusion induced pulmonary injury through modulating the miR-21-5p/Nr4a1 signaling pathway.

Apelin-13 protects the lungs from ischemia-reperfusion injury by attenuating inflammatory and oxidative stress

2020 ◽  
pp. 096032712096143
Author(s):  
F Xia ◽  
H Chen ◽  
Z Jin ◽  
Z Fu
Keyword(s):  
Oxidative Stress ◽  
Reperfusion Injury ◽  
Inflammatory Cytokines ◽  
Structural Damage ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Weight Ratio ◽  
Lung Edema ◽  
Sham Operation ◽  
Protective Effects

Apelin has been reported to regulate mitochondrial function in myocardial ischemia-reperfusion injury and cerebral ischemia-reperfusion injury. However, the role of apelin-13 in lung ischemia-reperfusion injury (LIRI) remains unclear. This study established an experimental rat model to evaluate the underlying mechanisms of apelin-13 on LIRI. Twenty-four rats were randomly divided to sham operation group (group SM), ischemia/reperfusion group (group IR), and apelin-13 treatment group (group APL). The effects of apelin-13 on LIRI were determined histologically using H&E staining, while the wet/dry weight ratio was used to assess lung edema caused by LIRI. Inflammatory cytokines were also detected in Bronchoalveolar lavage (BAL) fluid by ELISA. The protein expression of UCP2 and the morphological changes of mitochondria were determined by western blotting and electromicroscopy, respectively. The results demonstrated the structural damage of lung tissues and lung edema in group IR. An increased level of inflammatory cytokines including IL-1β, IL-6 and TNF-α was observed in rats with LIRI using ELISA. After that, oxidative stress and morphological damage of mitochondria were also shown in group IR. Yet, the application of apelin-13 reversed all these deleterious effects in group APL. The protective effects of apelin-13 were indicated by decreased reactive oxygen species (ROS) and elevated UCP2 expression levels in rats. In conclusion, this study revealed that apelin-13 had protective effects against LIRI via attenuating lung edema, the production of inflammatory cytokines, oxidative stress and mitochondrial dysfunction.

Sign in / Sign up

Export Citation Format

Share Document