Micropatterning of Poly (Ethylene Glycol)-Diacrylate (PEG-DA) Hydrogel by Soft-Photolithography for Analysis of Cell-Biomaterial Interactions

2009 ◽  
Vol 2 ◽  
pp. 3-14 ◽  
Author(s):  
Karthikeyan Subramani ◽  
M.A. Birch
Keyword(s):  
Ethylene Glycol ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Contact Printing ◽  
Pdms Stamp ◽  
Glycol Diacrylate ◽  
Dimethyl Siloxane ◽  
Hydrogel Matrix ◽  
Poly Ethylene ◽  
Innovative Techniques

Poly (ethylene glycol) hydrogel (PEG) micropatterns fabricated by photolithography and various other microfabrication techniques have been used as a platform to analyze cell-biomaterial interactions in cell culture studies. Numerous innovative techniques have been described about photolithography and the use of Poly (dimethyl siloxane) stamp (PDMS) based pressure moulding technique for the microfabrication of PEG hydrogel micropatterns. We herein this literature describe a simple and a versatile method for fabricating Poly (ethylene glycol) hydrogel-diacrylate (PEG-DA) hydrogel micropatterns using the ‘Soft-photolithography’ technique which is a combination of pressure moulding using a PDMS stamp and photolithography. Using this simple technique, PEG-DA hydrogel micropatterns were fabricated on a silicon substrate of varying dimensions from 40μm to 10μm within the same substrate. Such a three-dimensional microenvironment with varying sizes can serve as an excellent platform to study cell behaviour in culture. These PEG-DA hydrogel micropatterns can further be functionalized by adding a variety of biomolecular cues within the PEG-DA hydrogel matrix or these biomolecules can be patterned on the PEG-DA micropatterns after photopolymerization using micro-contact printing for analysis of cell-biomaterial interactions and tissue engineering purposes.

Culturing of transgenic mice liver tissue slices in three-dimensional microfluidic structures of PEG-DA (poly(ethylene glycol) diacrylate)

2013 ◽  
Vol 176 ◽  
pp. 1081-1089 ◽  
Author(s):  
Shilpa Sivashankar ◽  
Srinivasu Valegerahally Puttaswamy ◽  
Ling-Hui Lin ◽  
Tz-Shuian Dai ◽  
Chau-Ting Yeh ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Ethylene Glycol ◽  
Liver Tissue ◽  
Three Dimensional ◽  
Poly Ethylene Glycol ◽  
Tissue Slices ◽  
Glycol Diacrylate ◽  
Poly Ethylene ◽  
Mice Liver

scholarly journals A Combined Approach of Double Network Hydrogel and Nanocomposites Based on Hyaluronic Acid and Poly(ethylene glycol) Diacrylate Blend

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2454 ◽  
Author(s):  
Alfredo Ronca ◽  
Ugo D’Amora ◽  
Maria Raucci ◽  
Hai Lin ◽  
Yujiang Fan ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Sol Gel ◽  
Poly Ethylene Glycol ◽  
Double Network ◽  
Glycol Diacrylate ◽  
Physico Chemical ◽  
Hydrogel Matrix ◽  
Poly Ethylene ◽  
Sol Gel Synthesis

In this study, an innovative polymer blend, based on double network (DN) approach, has been developed by integrating a poly(ethylene glycol) diacrylate (PEGDA) network into a chemically modified hyaluronic acid sodium salt (HAs) hydrogel matrix. Here, the HAs was chemically functionalized with photocrosslinkable moieties by reacting with maleic anhydride (MAA) to obtain a maleated hyaluronic acid (MaHA). Furthermore, nanocomposite DN hydrogels were suitably prepared by physical blending of hydroxyapatite nanoparticles (HAp), obtained by sol-gel synthesis, within the hydrogel. Physico-chemical, thermal, morphological and mechanical analyses were performed. Results showed enhanced mechanical properties and a homogenous microstructure as highlighted by mechanical and morphological investigations. This suggests that nanocomposite DN hydrogels are promising candidates for biomedical applications.

3D Printing of Robust and Biocompatible Poly(ethylene glycol) Diacrylate/Nano-Hydroxyapatite Composites via Continuous Liquid Interface Production

2021 ◽  
Author(s):  
Xueyong Deng ◽  
Bingxue Huang ◽  
Rui Hu ◽  
Liling Chen ◽  
Yingying Tang ◽  
...  
Keyword(s):  
3D Printing ◽  
Ethylene Glycol ◽  
Three Dimensional ◽  
Liquid Interface ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printing Technology ◽  
Poly Ethylene ◽  
Continuous Liquid Interface Production ◽  
Speed And Accuracy

Three-dimensional (3D) printing technology with satisfied speed and accuracy has been a powerful force in biomaterial processing. Early studies on 3D printing of biomaterials mainly focus on their biocompatibility and...

scholarly journals Gel Polymer Electrolytes Based on Cross-Linked Poly(ethylene glycol) Diacrylate for Calcium-Ion Conduction

ACS Omega ◽  
2021 ◽  
Author(s):  
Saeid Biria ◽  
Shreyas Pathreeker ◽  
Francielli S. Genier ◽  
Fu-Hao Chen ◽  
Hansheng Li ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Polymer Electrolytes ◽  
Calcium Ion ◽  
Gel Polymer Electrolytes ◽  
Poly Ethylene Glycol ◽  
Ion Conduction ◽  
Glycol Diacrylate ◽  
Poly Ethylene

Effects of pore morphology and size on antimicrobial activity of chitosan/poly(ethylene glycol) diacrylate macromer semi-IPN hydrogels

2015 ◽  
Vol 132 (43) ◽  
pp. n/a-n/a ◽  
Author(s):  
Nazlı Sokmen Bedel ◽  
Melek Tezcan ◽  
Ozgur Ceylan ◽  
Gulten Gurdag ◽  
Huseyin Cicek
Keyword(s):  
Antimicrobial Activity ◽  
Ethylene Glycol ◽  
Pore Morphology ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
Poly Ethylene
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