Reconstructing Box-Like Bone Defect of Mandible with In Vivo Tissue Engineering Bone

2007 ◽  
Vol 330-332 ◽  
pp. 1165-1168
Author(s):  
Jin Feng Yao ◽  
Y.Z. Zhang ◽  
C.Y. Bao ◽  
L.Y. Sun ◽  
X.M. Hao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Graft ◽  
Bone Defect ◽  
Bone Defects ◽  
Fluorescence Labeling ◽  
X Ray ◽  
In Vivo Tissue Engineering ◽  
Living Bone ◽  
Massive Bone

The purpose of this study was to explore the feasibility of repairing massive bone defect with in vivo tissue engineering(TE) bone, and to provide experimental evidence for the application of in vivo TE bone into clinic in the future. Six calcium phosphate ceramics (Ca-P ceramics) columns were prepared, and then immersed in dynamic revised simulated body fluid (RSBF). 72 hours later, the bone-like apatite was formed on the surface and pore walls of ceramics. Three dogs were used in this study. Two ceramic columns were implanted bilaterally in the femoral muscles of each dog to construct living bone graft of in vivo TE bone. 6 weeks after implantation, they were transplanted to the box-like bone defects sites created in bilateral mandible of the same animals. The dogs were sacrificed at 8, 12 week after operation respectively. Samples were harvested for gross observation, X-ray examination, tetracycline fluorescence labeling, SPECT and histological observation. These results demonstrated that as a living bone graft, in vivo TE bone participated in the bone metabolism of host, and integrated with the host bone. It is feasible to reconstruct box-like bone defect of mandible with the in vivo TE bone.

Repairing Periodontal Bone Defect with In Vivo Tissue Engineering Bone

2007 ◽  
Vol 330-332 ◽  
pp. 1121-1124 ◽  
Author(s):  
Bi Zhang ◽  
X.J. Zhang ◽  
C.Y. Bao ◽  
Q. Wang ◽  
Jin Feng Yao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Graft ◽  
Bone Defects ◽  
Histological Observation ◽  
Post Transplantation ◽  
In Vivo Tissue Engineering ◽  
Periodontal Bone Defects ◽  
Empty Control ◽  
Group 1

The purpose of this study was to develop a feasible approach for repairing periodontal bone defects with the in vivo tissue engineering bone incorporated with bioabsorbable PLA membrane and to provide evidences for the clinical application. Osteoinductive HA/β-TCP sintered at 1100°C were implanted in the femur medial muscles in the hind legs of three dogs. Four weeks after implantation, the in vivo tissue engineering (TE) bone was explanted. Meanwhile, artificial periodontal bone defects of 8mm×6mm were performed on the buccal side of 4th premolar and 1st molar of mandible bilaterally, with the exposure of dental roots. The defects were treated as follows: (1) in vivo TE bone and PLA membrane; (2) HA/β-TCP ceramics and PLA membrane; (3) PLA membrane only; (4) empty control. At the 2, 4, 8 weeks post-transplantation, the dogs were sacrificed. The specimen were harvested and evaluated by gross inspection, dental radiography, SPECT (99mTC-MDP) and histological observation by MPIA2500. The results showed that more mature osseointegration was found in the group 1. We presumed that the in vivo TE bone graft could enhance the reparation of periodontal bone defects.

Reconstructing Box-Like Bone Defect of Mandible with In Vivo Tissue Engineering Bone

2007 ◽  
pp. 1165-1168
Author(s):  
Jin Feng Yao ◽  
Y.Z. Zhang ◽  
C.Y. Bao ◽  
L.Y. Sun ◽  
X.M. Hao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Defect ◽  
In Vivo Tissue Engineering

Implantation of bovine hydroxyapatite and secretome with different oxygen concentration may improve massive bone defect regeneration: An experimental study on animal model

2021 ◽  
pp. 088532822110518
Author(s):  
Taufin Warindra ◽  
Mouli Edward ◽  
Kukuh Dwiputra Hernugrahanto ◽  
Fedik Abdul Rantam ◽  
Ferdiansyah Mahyudin ◽  
...  
Keyword(s):  
Bone Defect ◽  
Tissue Bank ◽  
Hypoxic Condition ◽  
Bone Defects ◽  
Statistical Test ◽  
Metal Implants ◽  
Significant Difference ◽  
Bovine Hydroxyapatite ◽  
Massive Bone

The most widely used biomaterials in the treatment of massive bone defects are allograft bone or metal implants. The current problem is that the availability of allographs is limited and metal implants are very expensive. Mass production of secretome can make bone reconstruction of massive bone defects using a scaffold more effective and efficient. This study aims to prove bone regeneration in massive bone defects using bovine hydroxyapatite reconstruction with normoxic and hypoxic secretome conditions using collagen type 1 (COL1), alkaline phosphate (ALP), osteonectin (ON), and osteopontin (OPN) parameters. This is an in vivo study using male New Zealand white rabbits aged 6–9 months. The research was carried out at the Biomaterials Center—Tissue Bank, Dr. Soetomo Hospital for the manufacturer of bovine hydroxyapatite (BHA) and secretome BM-MSC culture under normoxic and hypoxic conditions, and UNAIR Tropical Disease Institute for implantation in experimental animals. Data analysis was carried out with the one-way ANOVA statistical test and continued with the Post Hoc test LSD statistical test to determine whether or not there were significant differences between groups. There were significant differences between hypoxic to normoxic group and hypoxic to BHA group at day-30 observation using ALP, COL 1, ON, and OPN parameters. Meanwhile, there is only osteonectin parameter has significant difference at day-30 observation. At day-60 observation, only OPN parameter has significant differences between hypoxic to normoxic and hypoxic to BHA group. Between day-30 and day-60 observation, BHA and normoxic groups have a significant difference at all parameters, but in hypoxic group, there are only difference at ALP, COL 1, and ON parameters. Hypoxic condition BM-MSC secretome with BHA composite is superior and could be an option for treating bone defect.

scholarly journals In VivoOsteogenesis of Vancomycin Loaded Nanohydroxyapatite/Collagen/Calcium Sulfate Composite for Treating Infectious Bone Defect Induced by Chronic Osteomyelitis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaojie Lian ◽  
Kezheng Mao ◽  
Xi Liu ◽  
Xiumei Wang ◽  
Fuzhai Cui
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Chronic Osteomyelitis ◽  
Rabbit Model ◽  
Calcium Sulphate ◽  
Bone Defects ◽  
Bone Graft Substitute ◽  
Calcium Sulphate Hemihydrate

A novel antibacterial bone graft substitute was developed to repair bone defects and to inhibit related infections simultaneously. This bone composite was prepared by introducing vancomycin (VCM) to nanohydroxyapatite/collagen/calcium sulphate hemihydrate (nHAC/CSH). XRD, SEM, and CCK-8 tests were used to characterize the structure and morphology and to investigate the adhesion and proliferation of murine osteoblastic MC3T3-E1 cell on VCM/nHAC/CSH composite. The effectiveness in restoring infectious bone defects was evaluatedin vivousing a rabbit model of chronic osteomyelitis. Ourin vivoresults implied that the VCM/nHAC/CSH composite performed well both in antibacterial ability and in bone regeneration. This novel bone graft substitute should be very promising for the treatment of bone defect-related infection in orthopedic surgeries.

scholarly journals In vivo experimental study of the arterial supply of the rabbit posterior limb

2021 ◽  
Vol 64 (6) ◽  
pp. 26-32
Author(s):  
Elena Pavlovschi ◽  
◽  
Alina Stoian ◽  
Grigore Verega ◽  
Viorel Nacu ◽  
...  
Keyword(s):  
Bone Graft ◽  
Contrast Material ◽  
Rabbit Model ◽  
Bone Defects ◽  
Allogeneic Bone ◽  
Posterior Limb ◽  
Circumflex Femoral Artery ◽  
Bone Segment ◽  
Massive Bone

Background: The use of bone graft has been a successful step in the treatment of a large number of diseases of the osteoarticular system. But a massive bone defect remains a dilemma for modern reconstructive surgery. Present methods used have a high level of morbidity and complication. Literature indicates the absence of an optimal solution in massive bone defects healing. The aim of this study: to perform an in vivo preliminary study of vascularization of the hind limb in the rabbit model, for obtaining a graft able for further inclusion in the host blood circulation, without immunosuppression by decellularization. Material and methods: The study was performed on the 12 laboratory rabbits. After euthanasia of the rabbit, the femoral and tibiofibular bone was collected without soft tissue, only with the vascular pedicle, and keeping the passage through the vessels. In the abdominal aorta was injected contrast material, with the subsequent preparation of the arterial vessels, succeeded by anatomical, morphological, radiography, and microangiography study of this vascularized bone segment. Results: The principal nutrient artery of the rabbit femur springs from the lateral circumflex femoral artery. The optimal segment for vascularized allografting (the rabbit model) was determined the upper third of the femur with the up to the level of the internal iliac artery. So, it could be used as a bone graft for further conservation and decellularization. Conclusions: The vascularized allogeneic bone without immunosuppression would be a perfect alternative in the treatment of the massive bone defects.

scholarly journals Reconstructing Bone with Natural Bone Graft: A Review of In Vivo Studies in Bone Defect Animal Model

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 999 ◽  
Author(s):  
Mengying Liu ◽  
Yonggang Lv
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Bone Structure ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Defects ◽  
Autologous Bone Graft ◽  
In Vivo Studies ◽  
Natural Bone

Bone defects caused by fracture, disease or congenital defect remains a medically important problem to be solved. Bone tissue engineering (BTE) is a promising approach by providing scaffolds to guide and support the treatment of bone defects. However, the autologous bone graft has many defects such as limited sources and long surgical procedures. Therefore, xenograft bone graft is considered as one of the best substitutions and has been effectively used in clinical practice. Due to better preserved natural bone structure, suitable mechanical properties, low immunogenicity, good osteoinductivity and osteoconductivity in natural bone graft, decellularized and demineralized bone matrix (DBM) scaffolds were selected and discussed in the present review. In vivo animal models provide a complex physiological environment for understanding and evaluating material properties and provide important reference data for clinical trials. The purpose of this review is to outline the in vivo bone regeneration and remodeling capabilities of decellularized and DBM scaffolds in bone defect models to better evaluate the potential of these two types of scaffolds in BTE. Taking into account the limitations of the state-of-the-art technology, the results of the animal bone defect model also provide important information for future design of natural bone composite scaffolds.

Repairing Periodontal Bone Defect with In Vivo Tissue Engineering Bone

2007 ◽  
pp. 1121-1124
Author(s):  
Bi Zhang ◽  
X.J. Zhang ◽  
C.Y. Bao ◽  
Q. Wang ◽  
Jin Feng Yao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Defect ◽  
In Vivo Tissue Engineering

The use of pimonidazole to characterise hypoxia in the internal environment of an in vivo tissue engineering chamber

2005 ◽  
Vol 58 (8) ◽  
pp. 1104-1114 ◽  
Author(s):  
S.O.P. Hofer ◽  
G.M. Mitchell ◽  
A.J. Penington ◽  
W.A. Morrison ◽  
R. RomeoMeeuw ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Internal Environment ◽  
In Vivo Tissue Engineering

Repairing Rabbit Radius Bone Defects with Simvastatin Compound Biological Bone

2021 ◽  
Vol 11 (7) ◽  
pp. 1263-1270
Author(s):  
Zhong-Yu Liu ◽  
Jin-Li Zhang ◽  
Yang Zhang ◽  
Shi-Lian Kan ◽  
Jun Liang ◽  
...  
Keyword(s):  
Bone Defect ◽  
Maximum Stress ◽  
Biomechanical Testing ◽  
Bone Defects ◽  
Bending Test ◽  
Bone Material ◽  
X Ray ◽  
Group A ◽  
Group B ◽  
Group D

Objective: This study aimed to investigate the feasibility of repairing rabbit radius bone defects with simvastatin compound biological bone. Methods: Simvastatin biological bone material was prepared, and osteoblasts were cultured. A total of 42 New Zealand white rabbits were randomly divided into four groups, and a bone defect with a length of 15 mm was created at the middle part of the radial shaft of both limbs in each rabbit, thereby establishing a bone defect model. The grafts in group A were biological bones of osteoblasts combined with simvastatin; the grafts in group B were biological bones of simvastatin; the grafts in group C were biological compound bones of osteoblasts; and the grafts in group D were simple biological bones. In each group, four animals were randomly sacrificed at the sixth and twelfth week after surgery, and specimens were collected for gross observation, X-ray examination, histological observation, and biomechanical testing. In each group, two animals were randomly sacrificed at the twelfth week after surgery; a three-point bending test was performed using a biomechanical testing machine, and the results were compared with those of a normal radius. Results: The X-ray and histological examinations at 6 and 12 weeks after surgery revealed that the osteogenesis ability of the simvastatin biological bone and osteoblast-simvastatin biological bone was better than that of the osteoblast biological bone and simple biological bone, which was superior in group A and group B to group C and group D. The results of the biomechanical examination revealed that the maximum stress of the normal radius was significantly higher than that of the experimental groups. Among the experimental groups, the difference between group A and group B was not statistically significant, and the maximum stress was higher in groups A and B than in groups C and D. Conclusion: Simvastatin biological bone material can promote the repair of rabbit radius defects and increase the quality of bone healing.

scholarly journals The Arteriovenous Loop: Engineering of Axially Vascularized Tissue

2018 ◽  
Vol 59 (3-4) ◽  
pp. 286-299 ◽  
Author(s):  
Annika Weigand ◽  
Raymund E. Horch ◽  
Anja M. Boos ◽  
Justus P. Beier ◽  
Andreas Arkudas
Keyword(s):  
Tissue Engineering ◽  
Large Scale ◽  
Treatment Options ◽  
Current Treatment ◽  
Loop Model ◽  
Engineering Approach ◽  
In Vivo Tissue Engineering ◽  
Tissue Engineering Approach ◽  
Tissue Defects

Background: Most of the current treatment options for large-scale tissue defects represent a serious burden for the patients, are often not satisfying, and can be associated with significant side effects. Although major achievements have already been made in the field of tissue engineering, the clinical translation in case of extensive tissue defects is only in its early stages. The main challenge and reason for the failure of most tissue engineering approaches is the missing vascularization within large-scale transplants. Summary: The arteriovenous (AV) loop model is an in vivo tissue engineering strategy for generating axially vascularized tissues using the own body as a bioreactor. A superficial artery and vein are anastomosed to create an AV loop. This AV loop is placed into an implantation chamber for prevascularization of the chamber inside, e.g., a scaffold, cells, and growth factors. Subsequently, the generated tissue can be transplanted with its vascular axis into the defect site and anastomosed to the local vasculature. Since the blood supply of the growing tissue is based on the AV loop, it will be immediately perfused with blood in the recipient site leading to optimal healing conditions even in the case of poorly vascularized defects. Using this tissue engineering approach, a multitude of different axially vascularized tissues could be generated, such as bone, skeletal or heart muscle, or lymphatic tissues. Upscaling from the small animal AV loop model into a preclinical large animal model could pave the way for the first successful attempt in clinical application. Key Messages: The AV loop model is a powerful tool for the generation of different axially vascularized replacement tissues. Due to minimal donor site morbidity and the possibility to generate patient-specific tissues variable in type and size, this in vivo tissue engineering approach can be considered as a promising alternative therapy to current treatment options of large-scale defects.

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