scholarly journals In vivo experimental study of the arterial supply of the rabbit posterior limb

2021 ◽  
Vol 64 (6) ◽  
pp. 26-32
Author(s):  
Elena Pavlovschi ◽  
◽  
Alina Stoian ◽  
Grigore Verega ◽  
Viorel Nacu ◽  
...  
Keyword(s):  
Bone Graft ◽  
Contrast Material ◽  
Rabbit Model ◽  
Bone Defects ◽  
Allogeneic Bone ◽  
Posterior Limb ◽  
Circumflex Femoral Artery ◽  
Bone Segment ◽  
Massive Bone

Background: The use of bone graft has been a successful step in the treatment of a large number of diseases of the osteoarticular system. But a massive bone defect remains a dilemma for modern reconstructive surgery. Present methods used have a high level of morbidity and complication. Literature indicates the absence of an optimal solution in massive bone defects healing. The aim of this study: to perform an in vivo preliminary study of vascularization of the hind limb in the rabbit model, for obtaining a graft able for further inclusion in the host blood circulation, without immunosuppression by decellularization. Material and methods: The study was performed on the 12 laboratory rabbits. After euthanasia of the rabbit, the femoral and tibiofibular bone was collected without soft tissue, only with the vascular pedicle, and keeping the passage through the vessels. In the abdominal aorta was injected contrast material, with the subsequent preparation of the arterial vessels, succeeded by anatomical, morphological, radiography, and microangiography study of this vascularized bone segment. Results: The principal nutrient artery of the rabbit femur springs from the lateral circumflex femoral artery. The optimal segment for vascularized allografting (the rabbit model) was determined the upper third of the femur with the up to the level of the internal iliac artery. So, it could be used as a bone graft for further conservation and decellularization. Conclusions: The vascularized allogeneic bone without immunosuppression would be a perfect alternative in the treatment of the massive bone defects.

scholarly journals In VivoOsteogenesis of Vancomycin Loaded Nanohydroxyapatite/Collagen/Calcium Sulfate Composite for Treating Infectious Bone Defect Induced by Chronic Osteomyelitis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaojie Lian ◽  
Kezheng Mao ◽  
Xi Liu ◽  
Xiumei Wang ◽  
Fuzhai Cui
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Calcium Sulfate ◽  
Chronic Osteomyelitis ◽  
Rabbit Model ◽  
Calcium Sulphate ◽  
Bone Defects ◽  
Bone Graft Substitute ◽  
Calcium Sulphate Hemihydrate

A novel antibacterial bone graft substitute was developed to repair bone defects and to inhibit related infections simultaneously. This bone composite was prepared by introducing vancomycin (VCM) to nanohydroxyapatite/collagen/calcium sulphate hemihydrate (nHAC/CSH). XRD, SEM, and CCK-8 tests were used to characterize the structure and morphology and to investigate the adhesion and proliferation of murine osteoblastic MC3T3-E1 cell on VCM/nHAC/CSH composite. The effectiveness in restoring infectious bone defects was evaluatedin vivousing a rabbit model of chronic osteomyelitis. Ourin vivoresults implied that the VCM/nHAC/CSH composite performed well both in antibacterial ability and in bone regeneration. This novel bone graft substitute should be very promising for the treatment of bone defect-related infection in orthopedic surgeries.

Reconstructing Box-Like Bone Defect of Mandible with In Vivo Tissue Engineering Bone

2007 ◽  
Vol 330-332 ◽  
pp. 1165-1168
Author(s):  
Jin Feng Yao ◽  
Y.Z. Zhang ◽  
C.Y. Bao ◽  
L.Y. Sun ◽  
X.M. Hao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Graft ◽  
Bone Defect ◽  
Bone Defects ◽  
Fluorescence Labeling ◽  
X Ray ◽  
In Vivo Tissue Engineering ◽  
Living Bone ◽  
Massive Bone

The purpose of this study was to explore the feasibility of repairing massive bone defect with in vivo tissue engineering(TE) bone, and to provide experimental evidence for the application of in vivo TE bone into clinic in the future. Six calcium phosphate ceramics (Ca-P ceramics) columns were prepared, and then immersed in dynamic revised simulated body fluid (RSBF). 72 hours later, the bone-like apatite was formed on the surface and pore walls of ceramics. Three dogs were used in this study. Two ceramic columns were implanted bilaterally in the femoral muscles of each dog to construct living bone graft of in vivo TE bone. 6 weeks after implantation, they were transplanted to the box-like bone defects sites created in bilateral mandible of the same animals. The dogs were sacrificed at 8, 12 week after operation respectively. Samples were harvested for gross observation, X-ray examination, tetracycline fluorescence labeling, SPECT and histological observation. These results demonstrated that as a living bone graft, in vivo TE bone participated in the bone metabolism of host, and integrated with the host bone. It is feasible to reconstruct box-like bone defect of mandible with the in vivo TE bone.

scholarly journals Transplantation of an allogeneic bone graft in treatment of post-sternotomy massive bone loss defects

2015 ◽  
Vol 10 (S1) ◽  
Author(s):  
Martin Kaláb ◽  
Jan Karkoška ◽  
Milan Kamínek ◽  
Eva Matějková ◽  
Vladimír Lonský
Keyword(s):  
Bone Loss ◽  
Bone Graft ◽  
Allogeneic Bone ◽  
Massive Bone Loss ◽  
Massive Bone

Implantation of bovine hydroxyapatite and secretome with different oxygen concentration may improve massive bone defect regeneration: An experimental study on animal model

2021 ◽  
pp. 088532822110518
Author(s):  
Taufin Warindra ◽  
Mouli Edward ◽  
Kukuh Dwiputra Hernugrahanto ◽  
Fedik Abdul Rantam ◽  
Ferdiansyah Mahyudin ◽  
...  
Keyword(s):  
Bone Defect ◽  
Tissue Bank ◽  
Hypoxic Condition ◽  
Bone Defects ◽  
Statistical Test ◽  
Metal Implants ◽  
Significant Difference ◽  
Bovine Hydroxyapatite ◽  
Massive Bone

The most widely used biomaterials in the treatment of massive bone defects are allograft bone or metal implants. The current problem is that the availability of allographs is limited and metal implants are very expensive. Mass production of secretome can make bone reconstruction of massive bone defects using a scaffold more effective and efficient. This study aims to prove bone regeneration in massive bone defects using bovine hydroxyapatite reconstruction with normoxic and hypoxic secretome conditions using collagen type 1 (COL1), alkaline phosphate (ALP), osteonectin (ON), and osteopontin (OPN) parameters. This is an in vivo study using male New Zealand white rabbits aged 6–9 months. The research was carried out at the Biomaterials Center—Tissue Bank, Dr. Soetomo Hospital for the manufacturer of bovine hydroxyapatite (BHA) and secretome BM-MSC culture under normoxic and hypoxic conditions, and UNAIR Tropical Disease Institute for implantation in experimental animals. Data analysis was carried out with the one-way ANOVA statistical test and continued with the Post Hoc test LSD statistical test to determine whether or not there were significant differences between groups. There were significant differences between hypoxic to normoxic group and hypoxic to BHA group at day-30 observation using ALP, COL 1, ON, and OPN parameters. Meanwhile, there is only osteonectin parameter has significant difference at day-30 observation. At day-60 observation, only OPN parameter has significant differences between hypoxic to normoxic and hypoxic to BHA group. Between day-30 and day-60 observation, BHA and normoxic groups have a significant difference at all parameters, but in hypoxic group, there are only difference at ALP, COL 1, and ON parameters. Hypoxic condition BM-MSC secretome with BHA composite is superior and could be an option for treating bone defect.

scholarly journals Preclinical Evaluation of an Innovative Bone Graft of Marine Origin for the Treatment of Critical-Sized Bone Defects in an Animal Model

2021 ◽  
Vol 11 (5) ◽  
pp. 2116
Author(s):  
Rafael Otero-Pérez ◽  
María Permuy ◽  
Estefanía López-Senra ◽  
Miriam López-Álvarez ◽  
Mónica López-Peña ◽  
...  
Keyword(s):  
Bone Graft ◽  
Low Cost ◽  
Rabbit Model ◽  
Bone Defects ◽  
Raw Material ◽  
Test Material ◽  
Micro Computed Tomography ◽  
Mineral Density ◽  
Preclinical Trial ◽  
Area Of Occupancy

Autogenous cancellous bone graft is the current gold standard of treatment for the management of bone defects since it possesses the properties of osteoinduction, osteoconduction, and osteogenesis. Xenografts and synthetic grafts have been widely reported as available and low-cost alternatives, which retain good osteoconductive and mechanical properties. Given the rich biodiversity of ocean organisms, marine sources are of particular interest in the search for alternative bone grafts with enhanced functionalities. The purpose of this paper is to assess the biocompatibility of a marine-derived bone graft obtained from shark tooth, which is an environmentally sustainable and abundant raw material from fishing. This research presents the findings of a preclinical trial—following UNE-EN ISO 10993—that induced a critical-sized bone defect in a rabbit model and compared the results with a commercial bovine-derived bone graft. Evaluation by micro-computed tomography and histomorphometric analysis 12 weeks after implantation revealed good osseointegration, with no signs of inflammatory foreign body reactions, fibrosis, or necrosis in any of the cases. The shark tooth-derived bone graft yielded significantly higher new bone mineral density values (54 ± 6%) than the control (27 ± 8%). Moreover, the percentage of intersection values were much higher (86 ± 8%) than the bovine-derived bone graft (30 ± 1%) used as control. The area of occupancy by bone tissue in the test material (38 ± 5%) also gave higher values than the control (30 ± 6%). The role of physicochemical properties, biphasic structure, and composition on the stimulation of bone regeneration is also discussed.

Evaluation of Formalin Preserved Allogeneic Bone Graft as a Grafting Material

2000 ◽  
Vol 30 (2) ◽  
pp. 76-78
Author(s):  
Sanjeev Agarwal ◽  
U S Mishra ◽  
U K Jain
Keyword(s):  
Bone Graft ◽  
Bone Defects ◽  
Allogeneic Bone ◽  
Fracture Union ◽  
Comminuted Fractures ◽  
Autologous Bone ◽  
Frozen Bone ◽  
Atrophic Nonunion

Formalin preserved allogeneic (FPA) bone graft was used in 18 patients with benign bone cavities, nine patients with malignant bone cavities, 12 patients with atrophic nonunion of fractures, seven with gap nonunion and 18 with comminuted fractures. The benign bone cavities were obliterated in 20 weeks and malignant cavities in 24 weeks. Union was obtained in atrophic ununited fractures in 29 weeks, in gap nonunion in 40 weeks and in comminuted fractures in 27 weeks. FPA is a simple, inexpensive and easily available alternative to autologous bone and to deep frozen bone in filling bone defects and enhancing fracture union.

scholarly journals Dipyridamole-loaded 3D-printed bioceramic scaffolds stimulate pediatric bone regeneration in vivo without disruption of craniofacial growth through facial maturity

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Maxime M. Wang ◽  
Roberto L. Flores ◽  
Lukasz Witek ◽  
Andrea Torroni ◽  
Amel Ibrahim ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Rabbit Model ◽  
Autologous Bone Graft ◽  
Autogenous Bone ◽  
Craniofacial Growth ◽  
Native Bone ◽  
3D Printed

AbstractThis study investigates a comprehensive model of bone regeneration capacity of dypiridamole-loaded 3D-printed bioceramic (DIPY-3DPBC) scaffolds composed of 100% beta-tricalcium phosphate (β –TCP) in an immature rabbit model through the time of facial maturity. The efficacy of this construct was compared to autologous bone graft, the clinical standard of care in pediatric craniofacial reconstruction, with attention paid to volume of regenerated bone by 3D reconstruction, histologic and mechanical properties of regenerated bone, and long-term safety regarding potential craniofacial growth restriction. Additionally, long-term degradation of scaffold constructs was evaluated. At 24 weeks in vivo, DIPY-3DPBC scaffolds demonstrated volumetrically significant osteogenic regeneration of calvarial and alveolar defects comparable to autogenous bone graft with favorable biodegradation of the bioactive ceramic component in vivo. Characterization of regenerated bone reveals osteogenesis of organized, vascularized bone with histologic and mechanical characteristics comparable to native bone. Radiographic and histologic analyses were consistent with patent craniofacial sutures. Lastly, through application of 3D morphometric facial surface analysis, our results support that DIPY-3DPBC scaffolds do not cause premature closure of sutures and preserve normal craniofacial growth. Based on this novel evaluation model, this DIPY-3DPBC scaffold strategy is a promising candidate as a safe, efficacious pediatric bone tissue engineering strategy.

scholarly journals Reconstructing Bone with Natural Bone Graft: A Review of In Vivo Studies in Bone Defect Animal Model

Nanomaterials ◽  
2018 ◽  
Vol 8 (12) ◽  
pp. 999 ◽  
Author(s):  
Mengying Liu ◽  
Yonggang Lv
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Bone Structure ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Bone Defects ◽  
Autologous Bone Graft ◽  
In Vivo Studies ◽  
Natural Bone

Bone defects caused by fracture, disease or congenital defect remains a medically important problem to be solved. Bone tissue engineering (BTE) is a promising approach by providing scaffolds to guide and support the treatment of bone defects. However, the autologous bone graft has many defects such as limited sources and long surgical procedures. Therefore, xenograft bone graft is considered as one of the best substitutions and has been effectively used in clinical practice. Due to better preserved natural bone structure, suitable mechanical properties, low immunogenicity, good osteoinductivity and osteoconductivity in natural bone graft, decellularized and demineralized bone matrix (DBM) scaffolds were selected and discussed in the present review. In vivo animal models provide a complex physiological environment for understanding and evaluating material properties and provide important reference data for clinical trials. The purpose of this review is to outline the in vivo bone regeneration and remodeling capabilities of decellularized and DBM scaffolds in bone defect models to better evaluate the potential of these two types of scaffolds in BTE. Taking into account the limitations of the state-of-the-art technology, the results of the animal bone defect model also provide important information for future design of natural bone composite scaffolds.

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