In Situ Investigation of Biomaterial Surfaces Using LDH and ELISA

Lactate dehydrogenase (LDH) and enzyme-linked immunosorbent assay (ELISA) have attracted much attention recently for the evaluation of blood compatibility of biomaterials due to their convenience and quantifiability. In this paper, the use of LDH and ELISA is described for in situ investigation of platelet behavior on biomaterial surfaces, including quantification of platelet adhesion and platelet activation, after suitable testing conditions have been established. The material samples investigated in these tests included low temperature isotropic carbon (LTIC), Ti-O films, and phosphorus- and aluminum-doped TiO2 films. The evaluation results show that the lowest platelet adhesion and activation are observed on phosphorus-doped TiO2 films while the highest platelet adhesion and activation are observed on LTIC. In addition, conventional platelet adhesion experiments were performed for comparison, and yielding similar evaluation results as LDH and ELISA. It is suggested that LDH and ELISA tests can be successfully applied to evaluate the blood compatibility of biomaterials and can show many advantages, such as quantification, reliability and objectivity, compared with conventional platelet adhesion test.

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Optoelectronic and photocatalytic properties of in situ platinum-doped TiO2 films deposited by means of pulsed laser ablation technique

Journal of Materials Science Materials in Electronics ◽

10.1007/s10854-016-5925-z ◽

2016 ◽

Vol 28 (4) ◽

pp. 3317-3324 ◽

Cited By ~ 2

Author(s):

K. Trabelsi ◽

A. Hajjaji ◽

I. Ka ◽

M. Gaidi ◽

B. Bessais ◽

...

Keyword(s):

Laser Ablation ◽

Pulsed Laser ◽

Pulsed Laser Ablation ◽

Photocatalytic Properties ◽

Tio2 Films ◽

Doped Tio2 ◽

Ablation Technique ◽

Laser Ablation Technique

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Antithrombogenic properties of Tulbaghia violacea–loaded polycaprolactone nanofibers

Journal of Bioactive and Compatible Polymers ◽

10.1177/0883911520903748 ◽

2020 ◽

Vol 35 (2) ◽

pp. 102-116

Author(s):

Lerato N Madike ◽

Michael Pillay ◽

Ketul C Popat

Keyword(s):

Medical Devices ◽

Plant Extracts ◽

Platelet Adhesion ◽

Blood Compatibility ◽

Fibre Diameter ◽

Electrospinning Technique ◽

Implantable Medical Devices ◽

Biomaterial Surfaces ◽

Rate Of Failure ◽

Novel Scaffold

A broad range of polymers have been utilized for the development of blood-contacting implantable medical devices; however, their rate of failure has raised the need for developing more hemocompatible biomaterial surfaces. In this study, a novel scaffold based on polycaprolactone incorporated with 10% and 15% (w/w) Tulbaghia violacea plant extracts were fabricated using electrospinning technique. The fabricated scaffolds were then treated with T. violacea aqueous plant extracts (100 and 1000 µg/mL) to investigate their use as interfaces for blood-contacting implants. The 10% Tvio scaffold produced the lowest mean fibre diameter (193 ± 30 nm), whereas the 15% Tvio scaffold produces the highest mean fibre diameter (538 ± 236 nm) when compared with the control polycaprolactone (275 ± 61 nm) scaffold. The number of adhered platelets was directly linked to fibre diameter and concentration of plant extract in such a way that the lowest fibre diameter scaffold (10% Tvio) inhibited platelet adhesion, whereas more platelets adhered to the scaffold with the highest fibre diameter (15% Tvio scaffolds). There was also an increase in platelet adhesion as the concentration of T. violacea was increased from 100 to 1000 µg/mL for all designed scaffolds. The improved blood compatibility demonstrated by the 10% Tvio scaffold suggests that the plant possesses antithrombogenic properties, particularly at lower concentrations.

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In Situ Investigation of Biomaterial Surfaces Using LDH and ELISA

Advanced Biomaterials VII - Key Engineering Materials ◽

10.4028/0-87849-436-7.841 ◽

2007 ◽

pp. 841-844

Author(s):

Zhen Yi Shao ◽

An Sha Zhao ◽

Ping Yang ◽

Hong Fang Zhou ◽

Nan Huang

Keyword(s):

In Situ Investigation ◽

Biomaterial Surfaces

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Investigation of Platelet Adhesion Behavior on Si-N-O Films Synthesized by Unbalanced Magnetron Sputtering

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.47-50.1407 ◽

2008 ◽

Vol 47-50 ◽

pp. 1407-1410

Author(s):

Zhen Yi Shao ◽

Ping Yang ◽

Yong Xiang Leng ◽

Qi Zhang ◽

Guo Jiang Wan ◽

...

Keyword(s):

Magnetron Sputtering ◽

Platelet Adhesion ◽

Blood Compatibility ◽

Single Crystal Silicon ◽

Silicon Substrates ◽

Crystal Silicon ◽

Unbalanced Magnetron Sputtering ◽

Unbalanced Magnetron ◽

Adhesion Behavior ◽

Isotropic Carbon

Si-N-O films have drawn researcher’s much attention recently due to their potential superiority in blood compatibility of biomaterials. In this paper, Si-N-O films were synthesized on <100> silicon substrates by pulsed reactive unbalanced magnetron sputtering a single crystal silicon target with high purity in a mixture atmosphere of Ar and N2. XPS and FTIR results showed the Si-N-O films synthesized at higher N2 flux could be described to random bonding model (RBM). In RBM, the Si2p existed in the form of a-Si3N4 and SiNνO4-ν (ν=0,1,2,3,4) components. Platelet adhesion behavior on Si-N-O films was assessed by platelet adhesion test and Lactate dehydrogenase (LDH) assay, qualitatively and quantitatively separately. The correlativity of film chemical structure and blood compatibility was investigated. The results of platelet adhesion and activation showed that the RBM film with higher N/O ratio exhibited favorable blood compatibility. It was shown that the Si-N-O film with specific composition and chemical bonding state was superior in blood compatibility compared to low temperature isotropic carbon (LTIC).

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In situ investigation of dye adsorption on TiO2 films using a quartz crystal microbalance with a dissipation technique

Physical Chemistry Chemical Physics ◽

10.1039/c2cp41268c ◽

2012 ◽

Vol 14 (25) ◽

pp. 9037 ◽

Cited By ~ 31

Author(s):

Hauke A. Harms ◽

Nicolas Tétreault ◽

Viktoria Gusak ◽

Bengt Kasemo ◽

Michael Grätzel

Keyword(s):

Quartz Crystal Microbalance ◽

Quartz Crystal ◽

Dye Adsorption ◽

Tio2 Films ◽

In Situ Investigation

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In situ investigation of the primary recrystallization of alpha titanium in HVEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110313 ◽

1978 ◽

Vol 36 (1) ◽

pp. 634-635

Author(s):

S. Naka ◽

R. Penelle ◽

R. Valle

Keyword(s):

Dimensional Analysis ◽

Texture Evolution ◽

Cold Work ◽

Three Dimensional ◽

Primary Recrystallization ◽

Thin Foils ◽

In Situ Investigation ◽

Grain Growth Model ◽

Alpha Titanium

The in situ experimentation technique in HVEM seems to be particularly suitable to clarify the processes involved in recrystallization. The material under investigation was unidirectionally cold-rolled titanium of commercial purity. The problem was approached in two different ways. The three-dimensional analysis of textures was used to describe the texture evolution during the primary recrystallization. Observations of bulk-annealed specimens or thin foils annealed in the microscope were also made in order to provide information concerning the mechanisms involved in the formation of new grains. In contrast to the already published work on titanium, this investigation takes into consideration different values of the cold-work ratio, the temperature and the annealing time.Two different models are commonly used to explain the recrystallization textures i.e. the selective grain growth model (Beck) or the oriented nucleation model (Burgers). The three-dimensional analysis of both the rolling and recrystallization textures was performed to identify the mechanismsl involved in the recrystallization of titanium.

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