Protein Adsorption of Highly (001) Oriented Hydroxyapatite Coating Using Rf-Plasma Spraying

2007 ◽  
Vol 361-363 ◽  
pp. 705-708 ◽  
Author(s):  
Masahiko Inagaki ◽  
Hisashi Nakashima ◽  
Takao Saito ◽  
Tetsuya Kameyama

The protein adsorption behavior was investigated for highly (001) oriented hydroxypatatite coatings (HACs). Highly (001) oriented (HO-) HACs and HAC with low orientation (LO-HAC) were prepared on titanium (Ti) substrates through a radio-frequency thermal plasma spraying method. Sintered HA pellets (S-HA) was also prepared as a control. The solution of 22 'g/100 'l PBS of the fluorescein-isothiocyanate (FITC) labeled bovine serum albumin (FITC-BSA), immunoglobulin G (FITC-IgG) and cytochrom c (FITC-CCC) was separately dropped on the surface of HACs and was incubated for 30 min. After the incubation, such HACs were washed with the PBS additionally supplemented with 125mM NaCl and observed using a fluorescence microscope. Fluorescence microscopic examination indicates that FITC labeled proteins somewhat adsorbed on the HACs, while proteins adsorbed little on S-HA surface. In particular, it can be seen that FITC-CCC adsorbed more prominently on the HO-HAC.

2006 ◽  
Vol 309-311 ◽  
pp. 615-618 ◽  
Author(s):  
Masahiko Inagaki ◽  
Yoshiyuki Yokogawa ◽  
Tetsuya Kameyama

Highly oriented hydroxyapatite (HA) coatings were prepared on titanium (Ti) substrates through a radio-frequency thermal plasma spraying method. XRD patterns showed that the HA coating layer had an structure with (00l) preferred crystal orientation. TEM observation showed that 200-800 nm-width prismatic crystals were formed in HA splats and the longitudinal axis of such prismatic crystals oriented vertical to the coating's surface. TEM images also indicate that the interface between prismatic crystals became compacted. SAD pattern show that the longitudinal axis of prismatic crystals corresponds to the <001> axis of HA.


2004 ◽  
Vol 45 (12) ◽  
pp. 3304-3308 ◽  
Author(s):  
Motohiro Yamada ◽  
Tatsuya Inamoto ◽  
Masahiro Fukumoto ◽  
Toshiaki Yasui

2000 ◽  
Author(s):  
I. Ahmed ◽  
T. L. Bergman

Abstract A 3D simulation of the thermal plasma spraying process is reported. In particular, the effect of the radial injection of a carrier gas is taken into account for a dilute spray. The thermal history of powder particles of different sizes is predicted. It is shown that introduction of a carrier gas can lead to a significant modification of the plasma jet, and can have an effect on the thermal histories of the injected particles. The study is motivated by the processing of non-traditional materials, specifically nanostructured ceramics.


2006 ◽  
Vol 47 (7) ◽  
pp. 1671-1676 ◽  
Author(s):  
Motohiro Yamada ◽  
Hajime Nakamura ◽  
Toshiaki Yasui ◽  
Masahiro Fukumoto ◽  
Koyata Takahashi

2005 ◽  
Vol 475-479 ◽  
pp. 2883-2886
Author(s):  
Heji Huang ◽  
Keisuke Eguchi ◽  
Makoto Kambara ◽  
Toyonobu Yoshida

With a newly established 300kW twin hybrid plasma spraying system, a peculiar layered composite zirconia coating was successfully deposited. The coating is consisting of splats and dendritic columns, which come from thermal plasma spraying and thermal plasma PVD, respectively. A 120µm-thick composite coating was deposited in 10 minutes at the corresponding growth rate of 100µm/min, if the rotation of the substrates is considered. The microstructure of such composite coatings has been characterized using a variety of microscopic techniques as part of a process optimization study.


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