Tissue Engineering Scaffolds of Bioceramics and New Bone Growth

2008 ◽  
Vol 368-372 ◽  
pp. 1161-1165 ◽  
Author(s):  
Jie Mo Tian ◽  
Li Min Dong ◽  
Chen Wang ◽  
Zhi Ping Guo ◽  
Chao Zong Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Growth ◽  
Experimental Results ◽  
Mineral Phase ◽  
Tissue Engineering Scaffolds ◽  
Before And After ◽  
Ft Ir

The paper describes β-TCP/DCHA and mineral phase structural bioceramics(CHA) as well as their 3-D structures, bioactivity, degradability and introducing new bone growth. FT-IR, XRD, SEM and Micro-CT were used to evaluate β-TCP/DCHA and mineral phase structural ceramics before and after implantation. Osteoblasts were immersed in the bioceramics and implanted in the rabbit femora. The experimental results showed that new bone grown in β-TCP/DCHA, and scaffolds were degraded with new bone formation and growth. The results indicated that β-TCP/DCHA was a better tissue engineering material. A kind of biomaterial (β-TCP/CHA) can be used for in situ formation or in vitro individuation formation. The experimental results indicated that β-TCP/CHA possessed better osteoblast affinity. Osteoblasts can adhere, proliferate and grow better on the material. The experiments in vivo showed the materials bonded with osseous tissue. The implants were degraded obviously after 6 months, and new bone replaced degradation materials.

Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

2012 ◽  
Vol 512-515 ◽  
pp. 1821-1825
Author(s):  
Lin Zhang ◽  
Xue Min Cui ◽  
Qing Feng Zan ◽  
Li Min Dong ◽  
Chen Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Cross Linking ◽  
Tissue Engineering Scaffolds ◽  
Composite Microspheres ◽  
Chitosan Composite ◽  
Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

scholarly journals Feasibility of Spatially Offset Raman Spectroscopy for in Vitro and in Vivo Monitoring Mineralization of Bone Tissue Engineering Scaffolds

2016 ◽  
Vol 89 (1) ◽  
pp. 847-853 ◽  
Author(s):  
Zhiyu Liao ◽  
Faris Sinjab ◽  
Amy Nommeots-Nomm ◽  
Julian Jones ◽  
Laura Ruiz-Cantu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Monitoring

scholarly journals Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair

2022 ◽  
Vol 9 ◽  
Author(s):  
Jiang-Nan Fu ◽  
Xing Wang ◽  
Meng Yang ◽  
You-Rong Chen ◽  
Ji-Ying Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Metal Composite ◽  
Tissue Engineering Scaffolds ◽  
Advantages And Disadvantages ◽  
Osteochondral Repair ◽  
Cell Growth And Differentiation ◽  
Manufacturing Technologies ◽  
Made In

Over centuries, several advances have been made in osteochondral (OC) tissue engineering to regenerate more biomimetic tissue. As an essential component of tissue engineering, scaffolds provide structural and functional support for cell growth and differentiation. Numerous scaffold types, such as porous, hydrogel, fibrous, microsphere, metal, composite and decellularized matrix, have been reported and evaluated for OC tissue regeneration in vitro and in vivo, with respective advantages and disadvantages. Unfortunately, due to the inherent complexity of organizational structure and the objective limitations of manufacturing technologies and biomaterials, we have not yet achieved stable and satisfactory effects of OC defects repair. In this review, we summarize the complicated gradients of natural OC tissue and then discuss various osteochondral tissue engineering strategies, focusing on scaffold design with abundant cell resources, material types, fabrication techniques and functional properties.

Faujasites Incorporated Tissue Engineering Scaffolds for Wound Healing: In Vitro and In Vivo Analysis

2013 ◽  
Vol 5 (21) ◽  
pp. 11194-11206 ◽  
Author(s):  
Neethu Ninan ◽  
Muthunarayanan Muthiah ◽  
In-Kyu Park ◽  
Anne Elain ◽  
Tin Wui Wong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Analysis

scholarly journals Cell Microenvironment Engineering and Monitoring for Tissue Engineering and Regenerative Medicine: The Recent Advances

2014 ◽  
Vol 2014 ◽  
pp. 1-18 ◽  
Author(s):  
Julien Barthes ◽  
Hayriye Özçelik ◽  
Mathilde Hindié ◽  
Albana Ndreu-Halili ◽  
Anwarul Hasan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Control Cell ◽  
Matrix Composition ◽  
Tissue Engineering Scaffolds ◽  
Cell Microenvironment ◽  
Engineered Tissues ◽  
Cellular Behaviour

In tissue engineering and regenerative medicine, the conditions in the immediate vicinity of the cells have a direct effect on cells’ behaviour and subsequently on clinical outcomes. Physical, chemical, and biological control of cell microenvironment are of crucial importance for the ability to direct and control cell behaviour in 3-dimensional tissue engineering scaffolds spatially and temporally. In this review, we will focus on the different aspects of cell microenvironment such as surface micro-, nanotopography, extracellular matrix composition and distribution, controlled release of soluble factors, and mechanical stress/strain conditions and how these aspects and their interactions can be used to achieve a higher degree of control over cellular activities. The effect of these parameters on the cellular behaviour within tissue engineering context is discussed and how these parameters are used to develop engineered tissues is elaborated. Also, recent techniques developed for the monitoring of the cell microenvironmentin vitroandin vivoare reviewed, together with recent tissue engineering applications where the control of cell microenvironment has been exploited. Cell microenvironment engineering and monitoring are crucial parts of tissue engineering efforts and systems which utilize different components of the cell microenvironment simultaneously can provide more functional engineered tissues in the near future.

Novel three Dimensional Biodegradable Scaffolds for Bone Tissue Engineering

1998 ◽  
Vol 550 ◽  
Author(s):  
Kacey G. Marra ◽  
Phil G. Campbell ◽  
Paul A. Dimilla ◽  
Prashant N. Kumta ◽  
Mark P. Mooney ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Bone Growth ◽  
Bone Cell ◽  
Degradation Rates

AbstractWe have constructed osteogenic scaffolds using solid freeform fabrication techniques. Blends of biodegradable polymers, polycaprolactone and poly(D,L-lactic-co-glycolic acid), have been examined as scaffolds for applications in bone tissue engineering. Hydroxyapatite granules were incorporated into the blends and porous discs were prepared. Mechanical properties and degradation rates of the composites were determined. The discs were seeded with rabbit bone marrow or cultured bone marrow stromal cells and in vitro studies were conducted. Electron microscopy and histological analysis revealed an osteogenic composite that supports bone cell growth not only on the surface but throughout the 1 mm thick scaffold as well. Seeded and unseeded discs were mechanically assembled in layers and implanted in a rabbit rectus abdominis muscle. Bone growth was evident after eight weeks in vivo. Electron microscopy and histological analyses indicate vascularization and primitive bone formation throughout the seeded composite, and also a “fusion” of the layers to form a single, solid construct. Finally, we have begun to incorporate the growth factor IGF-I into the scaffold to enhance osteogenicity and/or as an alternative to cell seeding.

scholarly journals Next-Generation Sequencing for Determining the Effect of Arginine on Human Dental Biofilms Using an In Situ Model

Pharmacy ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 18
Author(s):  
Nanako Kuriki ◽  
Yoko Asahi ◽  
Maki Sotozono ◽  
Hiroyuki Machi ◽  
Yuichiro Noiri ◽  
...  
Keyword(s):  
Periodontal Diseases ◽  
Bacterial Flora ◽  
Viable Count ◽  
Biofilm Model ◽  
Before And After ◽  
Oral Biofilms ◽  
Viable Bacteria

Oral biofilms are associated with caries, periodontal diseases, and systemic diseases. Generally, antimicrobial therapy is used as the first line of treatment for infectious diseases; however, bacteria in biofilms eventually develop antibiotic resistance. This study aimed to apply our in situ biofilm model to verify whether an arginine preparation is useful for plaque control. Ten healthy subjects who did not show signs of caries, gingivitis, or periodontitis were recruited. The dental biofilms from the subjects were obtained using our oral device before and after gargling with arginine solution for 4 weeks. We found that 8% arginine solution significantly increased the concentration of ammonium ions (NH4+) in vitro and in vivo in saliva (p < 0.05) and decreased the proportions of the genera Atopobium and Catonella in vivo. However, the viable count was unaffected by the mouthwash. Further, oral populations of the genera Streptococcus and Neisseria tended to increase with the use of arginine. Therefore, we concluded that using an 8% arginine solution decreased the NH4+ concentration in the oral cavity without affecting the number of viable bacteria, and that the diversity of oral bacterial flora changed. We suggest that arginine might help prevent mature biofilm formation.

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