scholarly journals Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair

2022 ◽  
Vol 9 ◽  
Author(s):  
Jiang-Nan Fu ◽  
Xing Wang ◽  
Meng Yang ◽  
You-Rong Chen ◽  
Ji-Ying Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Metal Composite ◽  
Tissue Engineering Scaffolds ◽  
Advantages And Disadvantages ◽  
Osteochondral Repair ◽  
Cell Growth And Differentiation ◽  
Manufacturing Technologies ◽  
Made In

Over centuries, several advances have been made in osteochondral (OC) tissue engineering to regenerate more biomimetic tissue. As an essential component of tissue engineering, scaffolds provide structural and functional support for cell growth and differentiation. Numerous scaffold types, such as porous, hydrogel, fibrous, microsphere, metal, composite and decellularized matrix, have been reported and evaluated for OC tissue regeneration in vitro and in vivo, with respective advantages and disadvantages. Unfortunately, due to the inherent complexity of organizational structure and the objective limitations of manufacturing technologies and biomaterials, we have not yet achieved stable and satisfactory effects of OC defects repair. In this review, we summarize the complicated gradients of natural OC tissue and then discuss various osteochondral tissue engineering strategies, focusing on scaffold design with abundant cell resources, material types, fabrication techniques and functional properties.

Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

2012 ◽  
Vol 512-515 ◽  
pp. 1821-1825
Author(s):  
Lin Zhang ◽  
Xue Min Cui ◽  
Qing Feng Zan ◽  
Li Min Dong ◽  
Chen Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Release Kinetics ◽  
In Vitro Release ◽  
Cross Linking ◽  
Tissue Engineering Scaffolds ◽  
Composite Microspheres ◽  
Chitosan Composite ◽  
Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

scholarly journals Feasibility of Spatially Offset Raman Spectroscopy for in Vitro and in Vivo Monitoring Mineralization of Bone Tissue Engineering Scaffolds

2016 ◽  
Vol 89 (1) ◽  
pp. 847-853 ◽  
Author(s):  
Zhiyu Liao ◽  
Faris Sinjab ◽  
Amy Nommeots-Nomm ◽  
Julian Jones ◽  
Laura Ruiz-Cantu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Raman Spectroscopy ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Monitoring

scholarly journals Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 797
Author(s):  
Georgia Kastania ◽  
Jack Campbell ◽  
Jacob Mitford ◽  
Dmitry Volodkin
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
Tissue Growth ◽  
Future Perspectives ◽  
Polyelectrolyte Multilayer ◽  
Advantages And Disadvantages ◽  
Cellular Behaviour ◽  
Straightforward Approach

Tissue engineering (TE) is a highly multidisciplinary field that focuses on novel regenerative treatments and seeks to tackle problems relating to tissue growth both in vitro and in vivo. These issues currently involve the replacement and regeneration of defective tissues, as well as drug testing and other related bioapplications. The key approach in TE is to employ artificial structures (scaffolds) to support tissue development; these constructs should be capable of hosting, protecting and releasing bioactives that guide cellular behaviour. A straightforward approach to integrating bioactives into the scaffolds is discussed utilising polyelectrolyte multilayer capsules (PEMCs). Herein, this review illustrates the recent progress in the use of CaCO3 vaterite-templated PEMCs for the fabrication of functional scaffolds for TE applications, including bone TE as one of the main targets of PEMCs. Approaches for PEMC integration into scaffolds is addressed, taking into account the formulation, advantages, and disadvantages of such PEMCs, together with future perspectives of such architectures.

Faujasites Incorporated Tissue Engineering Scaffolds for Wound Healing: In Vitro and In Vivo Analysis

2013 ◽  
Vol 5 (21) ◽  
pp. 11194-11206 ◽  
Author(s):  
Neethu Ninan ◽  
Muthunarayanan Muthiah ◽  
In-Kyu Park ◽  
Anne Elain ◽  
Tin Wui Wong ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Tissue Engineering Scaffolds ◽  
In Vivo Analysis

A review of biomaterials scaffold fabrication in additive manufacturing for tissue engineering

2019 ◽  
Vol 34 (6) ◽  
pp. 415-435 ◽  
Author(s):  
Tang Mei Shick ◽  
Aini Zuhra Abdul Kadir ◽  
Nor Hasrul Akhmal Ngadiman ◽  
Azanizawati Ma’aram
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
Material Properties ◽  
Computer Aided Design ◽  
Three Dimensional ◽  
Scaffold Fabrication ◽  
Manufacturing Techniques ◽  
Manufacturing Technologies

The current developments in three-dimensional printing also referred as “additive manufacturing” have transformed the scenarios for modern manufacturing and engineering design processes which show greatest advantages for the fabrication of complex structures such as scaffold for tissue engineering. This review aims to introduce additive manufacturing techniques in tissue engineering, types of biomaterials used in scaffold fabrication, as well as in vitro and in vivo evaluations. Biomaterials and fabrication methods could critically affect the outcomes of scaffold mechanical properties, design architectures, and cell proliferations. In addition, an ideal scaffold aids the efficiency of cell proliferation and allows the movements of cell nutrient inside the human body with their specific material properties. This article provides comprehensive review that covers broad range of all the biomaterial types using various additive manufacturing technologies. The data were extracted from 2008 to 2018 mostly from Google Scholar, ScienceDirect, and Scopus using keywords such as “Additive Manufacturing,” “3D Printing,” “Tissue Engineering,” “Biomaterial” and “Scaffold.” A 10 years research in this area was found to be mostly focused toward obtaining an ideal scaffold by investigating the fabrication strategies, biomaterials compatibility, scaffold design effectiveness through computer-aided design modeling, and optimum printing machine parameters identification. As a conclusion, this ideal scaffold fabrication can be obtained with the combination of different materials that could enhance the material properties which performed well in optimum additive manufacturing condition. Yet, there are still many challenges from the printing methods, bioprinting and cell culturing that needs to be discovered and investigated in the future.

Tissue Engineering Scaffolds of Bioceramics and New Bone Growth

2008 ◽  
Vol 368-372 ◽  
pp. 1161-1165 ◽  
Author(s):  
Jie Mo Tian ◽  
Li Min Dong ◽  
Chen Wang ◽  
Zhi Ping Guo ◽  
Chao Zong Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Growth ◽  
Experimental Results ◽  
Mineral Phase ◽  
Tissue Engineering Scaffolds ◽  
Before And After ◽  
Ft Ir

The paper describes β-TCP/DCHA and mineral phase structural bioceramics(CHA) as well as their 3-D structures, bioactivity, degradability and introducing new bone growth. FT-IR, XRD, SEM and Micro-CT were used to evaluate β-TCP/DCHA and mineral phase structural ceramics before and after implantation. Osteoblasts were immersed in the bioceramics and implanted in the rabbit femora. The experimental results showed that new bone grown in β-TCP/DCHA, and scaffolds were degraded with new bone formation and growth. The results indicated that β-TCP/DCHA was a better tissue engineering material. A kind of biomaterial (β-TCP/CHA) can be used for in situ formation or in vitro individuation formation. The experimental results indicated that β-TCP/CHA possessed better osteoblast affinity. Osteoblasts can adhere, proliferate and grow better on the material. The experiments in vivo showed the materials bonded with osseous tissue. The implants were degraded obviously after 6 months, and new bone replaced degradation materials.

scholarly journals Cell Microenvironment Engineering and Monitoring for Tissue Engineering and Regenerative Medicine: The Recent Advances

2014 ◽  
Vol 2014 ◽  
pp. 1-18 ◽  
Author(s):  
Julien Barthes ◽  
Hayriye Özçelik ◽  
Mathilde Hindié ◽  
Albana Ndreu-Halili ◽  
Anwarul Hasan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Control Cell ◽  
Matrix Composition ◽  
Tissue Engineering Scaffolds ◽  
Cell Microenvironment ◽  
Engineered Tissues ◽  
Cellular Behaviour

In tissue engineering and regenerative medicine, the conditions in the immediate vicinity of the cells have a direct effect on cells’ behaviour and subsequently on clinical outcomes. Physical, chemical, and biological control of cell microenvironment are of crucial importance for the ability to direct and control cell behaviour in 3-dimensional tissue engineering scaffolds spatially and temporally. In this review, we will focus on the different aspects of cell microenvironment such as surface micro-, nanotopography, extracellular matrix composition and distribution, controlled release of soluble factors, and mechanical stress/strain conditions and how these aspects and their interactions can be used to achieve a higher degree of control over cellular activities. The effect of these parameters on the cellular behaviour within tissue engineering context is discussed and how these parameters are used to develop engineered tissues is elaborated. Also, recent techniques developed for the monitoring of the cell microenvironmentin vitroandin vivoare reviewed, together with recent tissue engineering applications where the control of cell microenvironment has been exploited. Cell microenvironment engineering and monitoring are crucial parts of tissue engineering efforts and systems which utilize different components of the cell microenvironment simultaneously can provide more functional engineered tissues in the near future.

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