Biocompatibility of Carbonated Hydroxyapatite Nanoparticles with Different Crystallinities

Carbonated apatite (CHA) is commonly considered a promising synthetic material for biomedical applications in orthopedic and dental surgery due to its biocompatibility, bioresorption and bioactivity. CHA5, CHA37 and CHA90 powders were synthesized from wet method and the DRX patterns showed that the crystallinity and particle size of CHA samples increased proportionally with the synthesis temperature. Powder extracts medium were obtained from each sample to interact with MC3T3-E1 osteoblastics cells. It was evaluated morphology, citotoxicity, pH and Ca2+ concentration. Citotoxicity assays showed high metabolic activity on all samples when compared to control. The polygonal shaped and the confluent monolayer observed in control cells progressively changed according to the crystallinity increase of samples. Cells under mitosis and spindle-like shaped where the main alterations observed. In addition the cell viability could be sensitive to the acid reactivity and crystallinity of carbonated apatite samples.

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Effect of Synthesis Temperature on the Crystallization and Growth of In Situ Prepared Nanohydroxyapatite in Chitosan Matrix

ISRN Biomaterials ◽

10.1155/2014/897468 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 8

Author(s):

Habiba Elhendawi ◽

R. M. Felfel ◽

Bothaina M. Abd El-Hady ◽

Fikry M. Reicha

Keyword(s):

Particle Size ◽

Biomedical Applications ◽

Particle Growth ◽

Synthesis Temperature ◽

Molar Ratio ◽

Processing Temperature ◽

Hydroxyapatite Nanoparticles ◽

Chitosan Matrix ◽

Coprecipitation Technique

Hydroxyapatite nanoparticles (nHA) have been used in different biomedical applications where certain particle size distribution and morphology are required. Chitosan/hydroxyapatite (CS/HA) nanocomposites were prepared using in situ coprecipitation technique and the effect of the reaction temperature on the crystallization and particle growth of the prepared nanohydroxyapatite particles was investigated. The composites were prepared at different synthesis temperatures (−10, 37, and 60°C). XRD, FTIR, thermal analysis, TEM and SEM techniques were used to characterize the prepared specimens. It was found that the increase in processing temperature had a great affect on particle size and crystal structure of nHA. The low temperature (−10°C) showed inhabitation of the HA growth in c-direction and low crystallinity which was confirmed using XRD and electron diffraction pattern of TEM. Molar ratio of the bone-like apatite layer (Ca/P) for the nanocomposite prepared at 60°C was higher was higher than the composites prepared at lower temperatures (37 and −10°C).

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Ultrastructural aspects of gymnosperms reproductive organs tissues due to their functional condition

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083060 ◽

1978 ◽

Vol 36 (2) ◽

pp. 440-441

Author(s):

G. M. Kozubov

Keyword(s):

Metabolic Activity ◽

Functional Condition ◽

Reproductive Organs ◽

Considerable Change ◽

Complicated Structure ◽

Tetrad Stage ◽

Ubisch Bodies ◽

Similar Organization ◽

Female Reproductive Organs ◽

High Metabolic Activity

The ultrastructure of reproductive organs of pine, spruce, larch and ginkgo was investigated. It was found that the male reproductive organs possess similar organization. The most considerable change in the ultrastructure of the microsporocytes occur in meiosis. Sporoderm is being laid at the late tetrad stage. The cells of the male gameto-phyte are distinguished according to the metabolic activity of the or- ganells. They are most weakly developed in the spermiogenic cell. Ta-petum of the gymnosperms is of the periplasmodic - secretorial type. The Ubisch bodies which possess similar structure in the types investigated but are specific in details in different species are produced in tapetum.Parietal and subepidermal layers are distinguished for their high metabolic activity and are capable of the autonomous photosynthesis. Female reproductive organs differ more greatly in their struture and have the most complicated structure in primitive groups. On the first stages of their formation the inner cells of nucellus are transformed into the nucellar tapetum in which the structures similar to the Ubisch bodies taking part in the formation of the sporoderm of female gametophyte have been found.

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Effects on Cancellous Bone in the Metaphysis

Toxicologic Pathology ◽

10.1177/0192623317735787 ◽

2017 ◽

Vol 45 (7) ◽

pp. 876-878 ◽

Cited By ~ 2

Author(s):

Kathryn E. Gropp

Keyword(s):

Cancellous Bone ◽

Metabolic Activity ◽

Morphologic Changes ◽

High Metabolic Activity

Cancellous bone has high metabolic activity compared to many other bone compartments and can be affected not only by changes in physeal activity but also by perturbations in homeostasis caused by changes in physiology or on-target pharmacology. Examples of several types of resulting morphologic findings were presented; if known, the pathways causing morphologic changes were discussed.

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Effects of particle size and synthesis temperature on the structural properties of the Ni nanoparticles: Insights about the formation of the fcc-Ni structure

10.1063/1.3086209 ◽

2009 ◽

Cited By ~ 1

Author(s):

Edvaldo Alves de Souza ◽

Hebert Winnischofer ◽

Paula Haddad ◽

Tulio C. R. Costa ◽

Daniela Zanchet ◽

...

Keyword(s):

Particle Size ◽

Structural Properties ◽

Synthesis Temperature ◽

Ni Nanoparticles

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STARCH MEDIATED SYNTHESIS OF HYDROXYAPATITE NANOPARTICLE FOR BIOMEDICAL APPLICATIONS

Kongunadu Research Journal ◽

10.26524/krj58 ◽

2015 ◽

Vol 2 (1) ◽

pp. 15-17

Author(s):

Indira J

Keyword(s):

Biomedical Applications ◽

Soluble Starch ◽

Morphological Properties ◽

Spherical Nanoparticles ◽

Hydroxyapatite Nanoparticles ◽

X Ray Diffraction ◽

X Ray ◽

Ft Ir ◽

Average Size ◽

Controllable Size

Hydroxyapatite (HAP) nanoparticles with uniform morphologies and controllable size have been synthesized by template directed method. The environment and eco-friendly polysaccharide soluble starch is used as a template to regulate size and shape of the nanoparticles synthesized. Structural and morphological properties of as-synthesized hydroxyapatite nanoparticles have been examined through the techniques like Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD) and Scanning Electron Microscopy(SEM), respectively. The results indicate that the obtained particles are uniform discrete spherical nanoparticles. The average size of the hydroxyapatite nanoparticles were ranged from 45 to 60 nm.

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Green Synthesis of Hydroxyapatite Nanoparticles with Controlled Morphologies and Surface Properties Toward Biomedical Applications

Journal of Inorganic and Organometallic Polymers and Materials ◽

10.1007/s10904-019-01247-4 ◽

2019 ◽

Vol 30 (3) ◽

pp. 899-906 ◽

Cited By ~ 6

Author(s):

Sherif Elbasuney

Keyword(s):

Green Synthesis ◽

Surface Properties ◽

Biomedical Applications ◽

Hydroxyapatite Nanoparticles

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Lack of efficacy of echinocandins against high metabolic activity biofilms of Candida parapsilosis clinical isolates

Brazilian Journal of Microbiology ◽

10.1007/s42770-019-00219-7 ◽

2020 ◽

Vol 51 (3) ◽

pp. 1129-1133 ◽

Cited By ~ 2

Author(s):

Danilo Yamamoto Thomaz ◽

Marcia de Souza Carvalho Melhem ◽

João Nobrega de Almeida Júnior ◽

Gil Benard ◽

Gilda Maria Barbaro Del Negro

Keyword(s):

Metabolic Activity ◽

Candida Parapsilosis ◽

Clinical Isolates ◽

High Metabolic Activity

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Characterization and influence of hydroxyapatite nanopowders on living cells

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.9.286 ◽

2018 ◽

Vol 9 ◽

pp. 3079-3094 ◽

Cited By ~ 17

Author(s):

Przemyslaw Oberbek ◽

Tomasz Bolek ◽

Adrian Chlanda ◽

Seishiro Hirano ◽

Sylwia Kusnieruk ◽

...

Keyword(s):

Particle Size ◽

Biological Activity ◽

Physicochemical Properties ◽

Living Cells ◽

Great Promise ◽

Medical Applications ◽

Synthesis Methods ◽

Hydroxyapatite Nanoparticles ◽

Manufacturing Methods ◽

The Impact

Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and respiratory systems.

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