Evaluation of Protein Adsorption Capacity of TiO2-Supported Spherical Porous Hydroxyapatite

2017 ◽  
Vol 758 ◽  
pp. 90-96
Author(s):  
Max Katayama ◽  
Kengo Tamazawa ◽  
Ayu Kodaira ◽  
Toru Nonami
Keyword(s):  
Bovine Serum Albumin ◽  
Simulated Body Fluid ◽  
Protein Adsorption ◽  
Bovine Serum ◽  
Body Fluid ◽  
Porous Hydroxyapatite ◽  
Protein Bovine Serum Albumin ◽  
Representative Protein ◽  
Bsa Adsorption ◽  
Simulated Body Fluid Immersion

In this study, a novel titania-supported spherical porous hydroxyapatite (sHAp + TiO2) was prepared by compounding minute, uniquely shaped spherical porous hydroxyapatites (sHAp) with TiO2. Adsorption of the representative protein bovine serum albumin (BSA) was evaluated and simulated body fluid immersion experiments were carried out. Minimal adsorption of BSA to TiO2 was observed, and 33% and 25% adsorption to sHAp + TiO2 and sHAp were observed, respectively. The reduction in BSA concentration observed in the sHAp and sHAp + TiO2 solutions is presumably due to adsorption to HAp. It is highly likely that adsorption of the acidic protein BSA occurred at the sHAp Ca2+ site. In the simulated body fluid immersion experiments, there was greater expression of hydroxyapatite (HAp) on the surface of sHAp than there was on the surface of sHAp + TiO2. In the case of TiO2 alone, no HAp was produced, even after immersion for 3 days. These results suggest a relationship between BSA adsorption and the osteoconductivity of materials.

The Influence of Glucose and Bovine Serum Albumin on the Crystallization of a Bone-Like Apatite from Revised Simulated Body Fluid

2003 ◽  
Vol 254-256 ◽  
pp. 327-330 ◽  
Author(s):  
Sergey V. Dorozhkin ◽  
Elena I. Dorozhkina ◽  
Simeon Agathopoulos ◽  
José Maria F. Ferreira
Keyword(s):  
Bovine Serum Albumin ◽  
Simulated Body Fluid ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Body Fluid

Adsorption of Bovine Serum Albumin onto Metal Oxides: Adsorption Equilibrium and Kinetics onto Alumina and Zirconia

2010 ◽  
Vol 8 (1) ◽  
Author(s):  
Kadriye Bozgeyik ◽  
Turkan Kopac
Keyword(s):  
Bovine Serum Albumin ◽  
Metal Oxides ◽  
Protein Adsorption ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Order Kinetic ◽  
First Order ◽  
Pseudo First Order ◽  
Kinetics Of ◽  
Bsa Adsorption

In this study, the equilibrium and the kinetics of Bovine Serum Albumin (BSA) adsorption onto metal oxides such as alumina (Al2O3) and zirconia (ZrO2) were investigated in a batch reactor. The effects of pH and temperature to adsorption rate and the equilibrium were studied. The equilibrium process was described by the Langmuir or the Freundlich isotherm models. The maximum adsorption capacity (Q0) was found as 81.6 mg/g at pH 5 and 20°C for Al2O3 and 26 mg/g at pH 4 and 40°C for ZrO2, respectively. The protein adsorption capacity for ZrO2 increased with increasing pH and temperature, while the protein adsorption capacity of Al2O3 increased with increasing pH and decreasing temperature. The pseudo-first-order and pseudo-second-order kinetic models were used to describe the kinetic data. The rate kinetics of BSA adsorption onto Al2O3 and ZrO2 at all pH and temperatures were best fitted with the pseudo-first-order kinetic model. The electrostatic interactions of BSA with the metal oxide surface were found to be higher at pH 5 for Al2O3 and pH 4 for ZrO2.

Influence of Terahertz Radiation of Various Ranges on Molecule Conformation of Bovine Serum Albumin

2010 ◽  
Vol 5 (4) ◽  
pp. 182-185
Author(s):  
Angelina V. Kapralova ◽  
Aleksandr S. Pogodin
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Radiation Power ◽  
Uv Spectrophotometry ◽  
Absorption Bands ◽  
Conformation Changes ◽  
Protein Molecules ◽  
Molecule Conformation ◽  
Protein Bovine Serum Albumin

We investigated the influence of THz laser radiation of frequencies of 1.15 THz and 3.68 THz and radiation power of about 10 Mw on protein (bovine serum albumin). Using UV spectrophotometry, we revealed increase in the optical density of irradiated samples of bovine serum albumin at the characteristic absorption bands, which is evidence of conformation changes in protein molecules

scholarly journals Evaluation of Photocatalytic and Protein Adsorption Properties of Anodized Titanium Plate Immersed in Simulated Body Fluid

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Ryoji Sawada ◽  
Yuya Katou ◽  
Hirofumi Shibata ◽  
Max Katayama ◽  
Toru Nonami
Keyword(s):  
Sulfuric Acid ◽  
Simulated Body Fluid ◽  
Oxide Film ◽  
Protein Adsorption ◽  
Titanium Oxide ◽  
Body Fluid ◽  
Titanium Plate ◽  
Titanium Oxide Film ◽  
Adsorption Ability ◽  
Anodized Titanium

Titanium-based materials are widely used for implant treatments such as artificial dental roots. Surface treatment has the potential to improve not only the biocompatibility but also the chemical and mechanical durability of the surface without changing the mechanical properties of the metal. A relatively thick titanium oxide film can be formed by the anodic oxidation method. Phosphoric acid or sulfuric acid electrolytic solution has previously been used for anodic oxidation. Such anodized films have excellent film hardness, abrasion resistance, and adhesion. In this study, titanium plate was anodized using an aqueous solution of sulfuric acid in which titanium oxide powder was suspended. A 2800-nm-thick titanium oxide film was formed, which was thicker than that obtained using phosphoric acid electrolyte. The titanium plate was immersed in simulated body fluid for 1 day to evaluate the photocatalytic activity and protein adsorption ability, and a homogeneous crack-free hydroxyapatite layer was formed. This titanium plate showed high methylene blue bleaching capacity. The adsorption ability of the acidic protein of the anodized titanium plate subjected to the above treatment was high. This suggests that this titanium plate has antimicrobial properties and protein adsorption ability. Thus, we report that a titanium plate, anodized with a sulfuric acid aqueous electrolyte solution containing suspended TiO2 powder and immersed in simulated body fluid, might behave as an antibacterial and highly biocompatible implant material.

scholarly journals The Glass Transition Behavior of the Globular Protein Bovine Serum Albumin

2003 ◽  
Vol 85 (6) ◽  
pp. 3943-3950 ◽  
Author(s):  
Geoffrey J. Brownsey ◽  
Timothy R. Noel ◽  
Roger Parker ◽  
Stephen G. Ring
Keyword(s):  
Glass Transition ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Globular Protein ◽  
Transition Behavior ◽  
Protein Bovine Serum Albumin
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