Structural Modification of Styrene Maleic Anhydride Copolymers for Plant Bioactive Compound Extraction

2019 ◽  
Vol 798 ◽  
pp. 351-357
Author(s):  
P. Punyamoonwongsa ◽  
Pattana Kakumyan ◽  
Natsaran Saichana ◽  
Brian Tighe

Ability of poly (styrene-alt-maleic anhydride) (PSMA) to undergo a conformational transition into an amphipathic α-helix coil offers one possible mechanism by which PSMA surface activity can be switched on or off in response to the pH change. This behaviour allows it to be useful in membrane solubilization for extraction technology. Bioactive compounds are recovered from plant tissues for different reasons. One of the most important reasons is due to the increased demand in nutraceuticals market and modern therapeutics. Despite this, aqueous-based extraction of these compounds has been reported to give low extraction yield. A development of new green extraction protocol is still a challenging task for all researchers nowadays. This study demonstrated, for the first time, possible use of PSMA as a lysis agent for plant bioactive compound extraction. To enhance its membrane affinity at physiological pH, the polymer was esterified with methanol. Both PSMA and its derivative (ePSMA) were characterized in terms of their membrane binding affinity through a combined use of both surface characterization and physical techniques. Analysis of the ternary phase diagrams suggested that ePSMA could facilitate stronger hydrophobically-driven interactions with the lipid. This was convinced by the reduced critical PSMA/lipid mass ratio from 7:1 (PSMA) to 1:1 (ePSMA), as observed in the ternary phase diagrams. Last but not the least, the crude extracts of Coffea robusta leaves obtained from ePSMA-based extraction showed a total phenolic content of 20.32±0.75 mg/g sample, significantly higher than that from the PSMA- (14.24±1.27 mg/g sample) and aqueous-based (16.33±1.03 mg/g sample) extractions. A structural manipulation of PSMA is thus a key to tailor its membrane solubilization and so, the extraction efficacy of bioactive compounds from plant cells.

2013 ◽  
Vol 441 (1-2) ◽  
pp. 603-610 ◽  
Author(s):  
Giulia Bonacucina ◽  
Marco Cespi ◽  
Giovanna Mencarelli ◽  
Luca Casettari ◽  
Giovanni F. Palmieri

1999 ◽  
Vol 16 (3) ◽  
pp. 377-381 ◽  
Author(s):  
Suk-Young Choi ◽  
Seong-Geun Oh ◽  
Seong-Youl Bae ◽  
Sei-Ki Moon

2018 ◽  
Vol 18 (12) ◽  
pp. 7526-7532 ◽  
Author(s):  
Shaza Darwish ◽  
Jacek Zeglinski ◽  
Gamidi Rama Krishna ◽  
Rahamatullah Shaikh ◽  
Majeda Khraisheh ◽  
...  

2001 ◽  
Author(s):  
B b Ebbinghaus ◽  
O H Krikorian ◽  
E R Vance ◽  
M W Stewart

2021 ◽  
Vol 39 (4) ◽  
pp. 1066-1075
Author(s):  
K.K. Adama ◽  
U.P. Onochie ◽  
E. Gbeinzi

This study presents the application of ternary phase diagrams to tropical almond biodiesel components separation and purification at two temperatures. The seed oil was extracted mechanically and alkaline transesterified to produce biodiesel. The oil and biodiesel were characterized using standard methods. Tie lines and binodal solubility curve data were determined using modified cloud point titration procedures. Gas chromatographic method was employed in the analysis of the phase compositions. The mixture of biodiesel, methanol and glycerol were investigated at 20 °C, 30 °C and withdrawal times of 2 to 32 minutes at 2 minutes intervals. Distribution coefficient, K and solvent selectivity, S analysis were performed. Results obtained showed that S > 1 indicating the ability of methanol to promote phase separation and purification. K < 1 implying that there was lower quantities of methanol solubilized in the biodiesel phase. The ternary phase diagrams provided the means of predicting the components distribution. Keywords: Production, Characterization, Tropical Almond Biodiesel, Purification, Ternary Phase Diagram


1989 ◽  
Vol 4 (5) ◽  
pp. 1209-1217 ◽  
Author(s):  
K. Maex ◽  
G. Ghosh ◽  
L. Delaey ◽  
V. Probst ◽  
P. Lippens ◽  
...  

The thermodynamic equilibrium of structures consisting of a thin film silicide (TiSi2 or CoSi2) on doped Si (with As or B) is investigated. Isothermal sections of the ternary phase diagrams for Ti–Si–B, Co–Si–B, Ti–Si–As, and Co–Si–As have been evaluated, indicating the stability of high B concentrations in Si underneath a CoSi2 layer, the instability of high As concentrations in Si underneath a CoSi2 layer, and of B and As concentrations underneath a TiSi2 layer. The obtained thermodynamic predictions agree very well with experimental results (i) on the redistribution of dopants during silicide formation, (ii) on the diffusion of dopants from an ion implanted silicide, and (iii) on the stability of highly doped regions underneath the silicide, both for the case of TiSi2 and CoSi2. It is shown that even though the inaccuracy of reported thermodynamic data is substantial, thermodynamic calculations provide a useful guidance and are consistent with the experimental results.


Sign in / Sign up

Export Citation Format

Share Document