Experimental Substantiation of Antimicrobial Efficiency of a New Composite Polymeric Material Based on Poly(2-Hydroxyethyl Methacrylate) under the Action of Low-Intensity Current without External Power Supplies

2021 ◽  
Vol 1038 ◽  
pp. 154-161
Author(s):  
Vasyl Nagaichuk ◽  
Roman Chornopyshchuk ◽  
Igor Gerashchenko ◽  
Olena Kukolevska ◽  
Anatolie Sidorenko

Active use of polymeric materials has become an integral part of all areas of modern medicine. Wound dressings capable of prolonged release of drugs directly into the lesion occupy a special place among them. The possibility of using such materials in the presence of low-intensity currents without external power supplies in a comprehensive treatment program for patients with burn injuries remains promising. The aim of the work is to study experimentally the antimicrobial efficacy of a new composite polymeric material based on poly(2-hydroxyethyl methacrylate), saturated with the antiseptic decamethoxine, under conditions of low-intensity current without external power supplies. The method of free radical thermal polymerization of a mixture of liquid monomer 2-hydroxyethyl methacrylate, crosslinking agent triethylene glycol dimethacrylate, polymerization initiator azobisisobutyronitrile was used for the synthesis of composite polymeric material. In addition, fourfold volume of distilled water as a pore-forming agent and decamethoxine as an antimicrobial component were administered. Known dressings of synthetic and biological origin were selected for comparison, some of which were pre-soaked in a 0.02% solution of decamethoxine. The study of conductivity of the materials without external power supplies was performed on the surface of a dense nutrient medium in a Petri dish using VITA-01M measuring device. Determination of antibacterial properties was performed by diffusion into agar. The obtained results allowed to establish the ability of the suggested polymeric material to conduct low-intensity currents without external power supplies, exceeding the duration of other traditional dressings. Comparison of antimicrobial activity of the studied samples confirmed the synergism of the action of physical factors and a new polymer-based composite material with the addition of antimicrobial substance to inhibit the growth of the test museum and clinical strains of Staphylococcus aureus. The ability of low-intensity currents without external power supplies to potentiate the antimicrobial properties of a new composite polymeric material based on poly(2-hydroxyethyl methacrylate), modified with a pore-forming agent, with the addition of decamethoxine was experimentally established.

The Analyst ◽  
2016 ◽  
Vol 141 (12) ◽  
pp. 3898-3903 ◽  
Author(s):  
Maowei Dou ◽  
Juan Lopez ◽  
Misael Rios ◽  
Oscar Garcia ◽  
Chuan Xiao ◽  
...  

A low-cost b̲a̲ttery-powered s̲pectrophotometric s̲ystem (BASS) was developed for high-sensitivity point-of-care analysis in low-resource settings on a microfluidic chip without relying on external power supplies.


Author(s):  
А.В. Дерюгина ◽  
Н.В. Жемарина ◽  
М.Н. Иващенко ◽  
А.Г. Самоделкин ◽  
П.С. Игнатьев ◽  
...  

Срыв адаптационных возможностей организма может стать патогенетической основой различных заболеваний. Длительный выброс адреналина в кровь вызывает кататоксические эффекты, что делает актуальным поиск эффективных средств и методов коррекции гиперадреналинемии. Цель работы - изучение влияния низкоинтенсивного лазерного излучения (НИЛИ) на электрофоретическую подвижность эритроцитов, процессы липопероксидации и структуру надпочечников при адреналовой токсемии у крыс. Методика. Адреналовую токсемию моделировали внутрибрюшинным введением адреналина-гидрохлорида (0,1 мг/кг). Животным 1-й группы вводили только адреналин, животных 2-й - через 30 мин после введения адреналина облучали НИЛИ, крысы 3-й группы получали только воздействие НИЛИ, контролем служили интактные животные (4-я группа). Воздействие НИЛИ проводилось на затылочную область в течение 10 мин. В качестве источника излучения применяли аппарат лазерный терапевтический «Успех» (ГП «Восход»), основанный на низкоинтенсивном импульсном излучении (длина волны - 0,89±0,05 мкм, частота импульсов 415 Гц, плотность средней мощности излучения - 193 мкВт/см2, площадь облучаемой поверхности 20 см2). Забор крови производили через 1 ч, 24 ч и 1 нед после начала эксперимента. Определяли уровень малонового диальдегида по реакции с тиабарбитуровой кислотой; электрофоретическую подвижность эритроцитов изучали методом микроэлектрофореза. Структуру надпочечников изучали морфологическими методами. Результаты. Показано, что НИЛИ на фоне адреналина (2-я группа) через 1 нед нивелировало снижение электрофоретической подвижности эритроцитов, восстанавливало уровень малонового диальдегида до значений интактной группы. Морфологическая картина надпочечников в этой группе не отличалась от таковой у интактных животных, тогда как при действии адреналина (1-я группа) не происходило восстановления исследуемых показателей до физиологической нормы. Заключение. Полученные в ходе эксперимента данные свидетельствуют о корригирующем действии низкоинтенсивного лазерного излучения при адреналовой токсемии у животных. Механизм действия низкоинтенсивного лазерного излучения, вероятно, носит системный характер и связан с воздействием на стресс-реализующие системы организма. Maladaptation results in the third stage of stress, exhaustion, and, thus, may underlie many diseases. Prolonged release of epinephrine into blood exerts catatoxic effects; therefore, searching for effective methods for correction of hyperadrenalinemia is relevant. Presently, low-intensity laser therapy (LILT) is commonly used in medicine. However, the introduction of LILT to clinical practice is largely empirical. The aim of this study was to analyze the effect of LILT on electrokinetic potential of red blood cells (RBC), lipid peroxidation (LPO), and the morphology of adrenal glands in adrenal toxemia in rats. Methods. Adrenal toxemia was modeled by intraperitoneal administration of epinephrine hydrochloride (0.1 mg/kg) to rats. Animals of group 1 received epinephrine alone; animals of group 2 received LILT 30 min prior to epinephrine administration; rats of group 3 received LILT alone; and intact rats of group 4 were used as control. Blood was withdrawn at one hour, 24 h, and one week of the experiment onset. The RBC zeta potential, which characterizes the morpho-functional state of membranes, was determined by measuring the effect of low-intensity laser therapy on RBC electrophoretic mobility using microelectrophoresis. LPO as an indicator of cell membrane injury was determined by concentration of malonic dialdehyde (MDA). The structure of adrenal glands was studied histologically. Results. LILT combined with epinephrine (group 2) alleviated the decrease in RBC electrophoretic mobility and restored the MDA level to values of the intact group at one week. Morphological structure of adrenal glands in group 2 did not differ from that in control whereas epinephrine (group 1) did not restore the study parameters to physiologically normal values. Conclusion. The study showed that LILT exerted a correcting effect in experimental adrenal toxemia. The mechanism of this effect is likely systemic and related with the effect on stress systems of the body.


2018 ◽  
Vol 33 (9) ◽  
pp. 7759-7769 ◽  
Author(s):  
Yunlong Shang ◽  
Bing Xia ◽  
Naxin Cui ◽  
Chenghui Zhang ◽  
Chunting Chris Mi

2021 ◽  
Vol 7 (2) ◽  
pp. 331-334
Author(s):  
Laura E. Eisenhardt ◽  
Juliane Mayer ◽  
Peter P. Pott

Abstract Teeth grinding is, due to its various impacts on the human body, a highly discussed issue in dentistry. It can damage the tooth structure or cause pain due to muscle tension. At the moment, there is neither a satisfactory diagnostic nor a comprehensive treatment option. This paper deals with the development of an app-controlled, small, portable sensor unit that can be used by patients to monitor their teeth grinding in everyday life. It also offers a treatment option due to an implemented biofeedback option. To achieve the most cost-effective device possible, only off-the-shelf electronics and no proprietary software were used. In initial tests, the measuring device showed high level of measurement accuracy when performing measurements without feedback at rest (f_score=-0.025...0).


Author(s):  
V. S. Tryasunov ◽  
I. V. Lishevich ◽  
G. I. Nikolaev ◽  
E. L. Shultseva ◽  
V. E. Baruev ◽  
...  

The article considers approaches and requirements for fire-safety characteristics definition of composite polymeric materials in shipbuilding. The results of experimental determination for such characteristics for three-layer composite polymeric material and fiberglass layer as an example are provided. The need for the development of domestic regulatory documentation on the correct requirements for testing multilayer PCM and data interpretation has been identified.


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