Test Method for Skin Damage of Titania Photocatalyst Nanoparticles In Vitro

Nanoscale TiO2 is widely used in consumer products like sunscreen and cosmetics. The establishment of damage evaluation test method was attempted to examine the potential neurotoxicity of nanoscale TiO2 to human body skin in vitro model. The emergence amounts of carbon dioxide, which was expected one of the generation products from the skin according to the titania photocatalyst nanoparticles activity under UV / visible light radiation, were identified and measured by the gas analyzer. It was found that it could evaluate the degrees of damage to skin with the photocatalysts activity by using the new evaluation test method considered.

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An in vitro model to evaluate the impact of environmental fine particles (PM0.3-2.5) on skin damage

Toxicology Letters ◽

10.1016/j.toxlet.2019.01.016 ◽

2019 ◽

Vol 305 ◽

pp. 94-102 ◽

Cited By ~ 7

Author(s):

Anthony Verdin ◽

Fabrice Cazier ◽

Richard Fitoussi ◽

Natacha Blanchet ◽

Katell Vié ◽

...

Keyword(s):

In Vitro Model ◽

Fine Particles ◽

Skin Damage ◽

Vitro Model ◽

The Impact

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Test Method for Skin Damage of Titania Photocatalyst Nanoparticles In Vitro

Eco-Materials Processing and Design IX - Materials Science Forum ◽

10.4028/0-87849-472-3.9 ◽

2008 ◽

pp. 9-12

Author(s):

Eiji Watanabe ◽

Mitsuharu Fukaya ◽

Kaori Nishizawa ◽

Takeshi Miki ◽

Hiroshi Taoda

Keyword(s):

Test Method ◽

Skin Damage ◽

Titania Photocatalyst

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An in vitro model for investigation of vitamin A effects on wound healing

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000692 ◽

2021 ◽

pp. 1-6

Author(s):

Hoda Keshmiri Neghab ◽

Mohammad Hasan Soheilifar ◽

Gholamreza Esmaeeli Djavid

Keyword(s):

Wound Healing ◽

Endothelial Cell ◽

Vitamin A ◽

In Vitro Model ◽

Cellular Proliferation ◽

Endothelial Cell Migration ◽

Normal Fibroblast ◽

Anti Inflammatory ◽

Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inﬂammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inﬂammation responses.

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