scholarly journals A High-Throughput Method (ASE-GC/MS/MS/MRM) for Quantification of Multiple Hydrocarbon Compounds in Marine Environmental Samples

2018 ◽  
Vol 52 (6) ◽  
pp. 66-70 ◽  
Author(s):  
Isabel C. Romero
Keyword(s):  
High Throughput ◽  
Environmental Samples ◽  
Multiple Reaction Monitoring ◽  
Lipid Extraction ◽  
Single Step ◽  
Oxidation Products ◽  
Extraction And Purification ◽  
High Throughput Method ◽  
Multiple Reaction Monitoring Mode ◽  
Polycyclic Aromatic

AbstractA high-throughput method was applied in small and complex environmental samples for targeting multiple organic fractions (polar, nonpolar). The analytical method consists of a single-step lipid extraction and purification procedure (accelerated solvent extraction [ASE] system) coupled with a single-run step using gas chromatography with tandem mass spectrometry, operated in multiple reaction monitoring mode (GC/MS/MS/MRM). Successful application of this method is summarized for multiple chemical groups (aliphatics, terpanes, steranes, triaromatic steroids, polycyclic aromatic hydrocarbons, and oxidation products).

scholarly journals Determination of Choline, Betaine, and Dimethylglycine in Plasma by a High-Throughput Method Based on Normal-Phase Chromatography–Tandem Mass Spectrometry

2003 ◽  
Vol 49 (2) ◽  
pp. 286-294 ◽  
Author(s):  
Pål I Holm ◽  
Per Magne Ueland ◽  
Gry Kvalheim ◽  
Ernst A Lien
Keyword(s):  
Mass Spectroscopy ◽  
High Throughput ◽  
Multiple Reaction Monitoring ◽  
Plasma Concentrations ◽  
Normal Phase ◽  
Tandem Mass ◽  
Internal Standards ◽  
High Throughput Method ◽  
Tandem Mass Spectroscopy

Abstract Background: The quaternary ammonium compounds, choline and betaine, and dimethylglycine (DMG) reside along a metabolic pathway linked to the synthesis of neurotransmitters and membrane phospholipids and to homocysteine remethylation and, therefore, folate status. Lack of a convenient, high-throughput method for the determination of these compounds has prevented population-based studies of their possible associations with lifestyle, nutrition, and chronic diseases. Methods: Serum or plasma samples were deproteinized by mixing with three volumes of acetonitrile that contained d9-choline and d9-betaine as internal standards. We used a normal-phase silica column for the separation of choline (retention time, 2.8 min), betaine (1.3 min), DMG (1.15 min), and internal standards, which were detected as positive ions by tandem mass spectroscopy in the multiple-reaction monitoring mode, using the molecular transitions m/z 104→60 (choline), m/z 113→69 (d9-choline), m/z 118→59 (betaine), m/z 127→68 (d9-betaine), and m/z 104→58 (DMG). Results: For all three metabolites, the assay was linear in the range 0.4–400 μmol/L, and the lower limit of the detection (signal-to-noise ratio = 5) was ≤0.3 μmol/L. The within- and between-day imprecision (CVs) was 2.1–7.2% and 3.5–8.8%, respectively. The analytical recovery was 87–105%. The fasting plasma concentrations (median, 25th–75th percentiles) were 8.0 (7.0–9.3) μmol/L for choline, 31.7 (27.0–41.1) μmol/L for betaine, and 1.66 (1.30–2.02) μmol/L for DMG in 60 healthy blood donors. In individuals who had eaten a light breakfast, plasma concentrations of all three metabolites were significantly (25–30%) higher than in fasting individuals. Conclusion: This is the first method for the combined measurement of choline, betaine, and DMG in human plasma or serum. The assay is characterized by simple sample preparation, no derivatization, high throughput, imprecision (CV) <10%, detection limits below the values seen in volunteers, and the high specificity provided by tandem mass spectroscopy.

scholarly journals High-Throughput LC/MS/MS Analysis of Ruscogenin and Neoruscogenin in Ruscus aculeatus L.

2009 ◽  
Vol 92 (4) ◽  
pp. 1055-1059 ◽  
Author(s):  
Laurian Vlase ◽  
Béla Kiss ◽  
Georgeta Balica ◽  
Mircea Tas ◽  
Gianina Crisan ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Mass Spectrometer ◽  
High Throughput ◽  
Ion Trap ◽  
Multiple Reaction Monitoring ◽  
Reaction Monitoring ◽  
Ionization Source ◽  
Ion Trap Mass Spectrometer ◽  
Multiple Reaction Monitoring Mode ◽  
Ionization Mode

Abstract A new, sensitive LC/MS/MS method was developed for the quantification of ruscogenin and neoruscogenin in hydrolyzed extracts from Ruscus aculeatus L. (Liliaceae). The two sapogenins were separated on a Zorbax SB-C18 column under isocratic conditions. The detection was performed in the multiple reaction monitoring mode using an ion trap mass spectrometer with an electrospray ionization source operated in positive ionization mode. For the quantification of the ruscogenin and neoruscogenin, calibration curves were constructed over the range of 21000 ng/mL. This is the first reported LC/MS/MS method for the simultaneous analysis of ruscogenin and neoruscogenin, and it showed superior sensitivity when compared with other assays described in the literature. The method has been successfully applied to quantify the two sapogenins in aerial (phylloclades) and underground parts (rhizomes, roots) of Ruscus aculeatus L.

3D nanostructures fabricated by advanced stencil lithography

Nanoscale ◽  
2016 ◽  
Vol 8 (9) ◽  
pp. 4945-4950 ◽  
Author(s):  
F. Yesilkoy ◽  
V. Flauraud ◽  
M. Rüegg ◽  
B. J. Kim ◽  
J. Brugger
Keyword(s):  
High Throughput ◽  
Single Step ◽  
Metal Nanostructures ◽  
3D Nanostructures ◽  
High Throughput Method

We present a high-throughput method to fabricate versatile and complex 3D metal nanostructures using single-step nanostencil lithography.

A Fluorescent High Throughput Method To Identify Potential Skin Sensitizers

Planta Medica ◽  
2016 ◽  
Vol 82 (05) ◽  
Author(s):  
C Avonto ◽  
AG Chittiboyina ◽  
D Rua ◽  
IA Khan
Keyword(s):  
High Throughput ◽  
High Throughput Method

Continuous Ozonation of Polycyclic Aromatic Hydrocarbons in Oil/Water-Emulsions and Biodegradation of Oxidation Products

1999 ◽  
Vol 40 (4-5) ◽  
pp. 107-114 ◽  
Author(s):  
A. Kornmüller ◽  
U. Wiesmann
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Biological Treatment ◽  
Reaction Rate ◽  
Oxidation Products ◽  
Direct Reaction ◽  
Biological Degradation ◽  
Polycyclic Aromatic ◽  
Oil Water ◽  
Treatment Step

The continuous ozonation of polycyclic aromatic hydrocarbons (PAH) was studied in a two stage ozonation system followed by serobic biological degradation. The highly condensed PAH benzo(e)pyrene and benzo(k)fluoranthene were oxidized selectiely in synthetic oil/water-emulsions. The influence of the ozone mass transfer gas-liquid on the reaction rate of benzo(k)fluoranthene was studied for process optimization. The dissolved ozone concentration is influenced by temperature to a higher degree than the reaction rate of PAH. In dependence on pH, PAH oxidation occurs by a direct reaction with ozone inside the oil droplets. Two main ozonation products of benzo(e)pyrene were quantified at different retention times during ozonation and their transformation could be shown in the biological treatment step.

scholarly journals High Throughput Centrifugal Electrospinning of Polyacrylonitrile Nanofibers for Carbon Fiber Nonwovens

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1313
Author(s):  
Andreas Hoffmann ◽  
Alexander J. C. Kuehne
Keyword(s):  
High Throughput ◽  
Carbon Nanofiber ◽  
Fiber Diameter ◽  
Carbon Precursor ◽  
Production Methods ◽  
High Throughput Method ◽  
Radial Forces ◽  
Fiber Deposition ◽  
The Impact ◽  
Pan Fibers

Carbon nanofiber nonwovens are promising materials for electrode or filtration applications; however, their utilization is obviated by a lack of high throughput production methods. In this study, we utilize a highly effective high-throughput method for the fabrication of polyacrylonitrile (PAN) nanofibers as a nonwoven on a dedicated substrate. The method employs rotational-, air pressure- and electrostatic forces to produce fibers from the inner edge of a rotating bell towards a flat collector. We investigate the impact of all above-mentioned forces on the fiber diameter, morphology, and bundling of the carbon-precursor PAN fibers. The interplay of radial forces with collector-facing forces has an influence on the uniformity of fiber deposition. Finally, the obtained PAN nanofibers are converted to carbon nonwovens by thermal treatment.

scholarly journals A neural network-based algorithm for high-throughput characterisation of viscoelastic properties of flowing microcapsules

Soft Matter ◽  
2021 ◽  
Author(s):  
Tao Lin ◽  
Zhen Wang ◽  
Wen Wang ◽  
Yi Sui
Keyword(s):  
Neural Network ◽  
High Throughput ◽  
Viscoelastic Properties ◽  
Dynamic Deformation ◽  
Membrane Elasticity ◽  
Hybrid Neural Network ◽  
High Throughput Method

We have developed a high-throughput method, by combining a hybrid neural network with a mechanistic capsule model, to predict membrane elasticity and viscosity of microcapsules from their dynamic deformation in a branched microchannel.

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