Fruit morphology helps identifying evolutionary groups in Alpinieae (Zingiberaceae): inferences from phylogenetic analysis of gingers in Sri Lanka

Until now, four species of Clidicus Laporte were found in Sri Lanka, three known from female specimens only. Clidicus minilankanus sp. n., is described, and compared to all remaining sympatric congeners. The Sri Lankan species may form a monophyletic group characterized by several morphological oddities: the head only slightly impressed posteromedially, with a large portion of vertex and frons not divided longitudinally; the pronotum quadrangular and flattened, with vestigial or absent posterior ‘collar’, and the transverse groove that demarcates it from the disc lacking pits or even entirely or partly obliterated; and the metaventrite strongly shortened, so that meso- and metacoxae are nearly adjacent. These characters may justify resurrecting Erineus Walker, a junior synonym of Clidicus (proposed for the first described Sri Lankan species, C. monstrosus (Walker)), as a valid name for a subgenus. This problem must be addressed by a phylogenetic analysis of all Clidicus species, to establish evolutionary relationships within this interesting genus.

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Isolation, molecular characterization and phylogeny of Naegleria species in water bodies of North-Western Province, Sri Lanka

PLoS ONE ◽

10.1371/journal.pone.0248510 ◽

2021 ◽

Vol 16 (3) ◽

pp. e0248510

Author(s):

Nuwan Gunarathna ◽

Anjalie Amarasinghe ◽

Sunil Wijesundara ◽

Devika Iddawela ◽

Susiji Wickramasinghe

Keyword(s):

Phylogenetic Analysis ◽

Sri Lanka ◽

Bayesian Analysis ◽

Molecular Characterization ◽

Its Region ◽

Water Bodies ◽

Internal Transcribed Spacer Region ◽

Western Province ◽

The North ◽

North Western

Background The inland freshwater bodies in the North-Western Province of Sri Lanka have ideal environmental conditions for the Naegleria species. Therefore, the presence and prevalence of Naegleria species in the water bodies of North-Western Province were determined by molecular characterization and phylogenetic analysis in this study. Methods A total of 104 water bodies were selected from Kurunegala and Puttalam districts in the North-Western Province of Sri Lanka. Mean turbidity, pH, and temperature were recorded in each water body from three selected site. Centrifuged samples were cultured on non-nutrient agar plates with Escherichia coli. Enflagellation test positive isolates were subjected to DNA extraction and polymerase chain reaction using genus and species-specific primers targeting the internal transcribed spacer region (ITS) and Mp2CL5 gene. Phylogenetic analysis was performed using Bayesian and maximum likelihood (ML) methods. Results The prevalence of Naegleria species and N. fowleri in the study area were 23.07% and 1.92%. The prevalence of Naegleria species and the physicochemical parameters of the water bodies showed no significant correlation. Bayesian analysis of the ITS region revealed the Naegleria Sri Lankan (SL) isolates 1, 3, and 4 in a single clade separated from the 2 and 5. Furthermore, Bayesian analysis identified isolates 2 and 5 in the same clade with Naegleria sp. samples and N. Philippinensis forming a sister clade. However, in the ML tree, all isolates were in the same clade with Naegleria sp. samples and N. Philippinensis. Conclusions The present study reports the first isolation of pathogenic N. fowleri from Sri Lanka. Based on Bayesian analysis, SL isolates 2 and 5 form a separate clade from 1, 3, and 4. However, in ML analysis, all isolates are grouped in one clade with Naegleria sp. samples and N. philippinensis. Further investigations are required to confirm these findings.

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Green-Spinach, Red-Spinach, and Tree-Spinach (‘Three-Fold Spinach’ in Sri Lanka): An Insight into Phylogenetics and Consumer Preference

Emirates Journal of Food and Agriculture ◽

10.9755/ejfa.2020.v32.i2.2065 ◽

2020 ◽

pp. 82

Author(s):

T. H. I. Gayathree ◽

S. I. Karunarathne ◽

L. T. Ranaweera ◽

H. S. M. Jayarathne ◽

S. K. Kannangara ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Sri Lanka ◽

Genomic Dna ◽

Evolutionary Relationship ◽

Consumer Preference ◽

Basella Alba ◽

The Poor ◽

Definition Of ◽

Relationship Of ◽

Insight Into

Three types of spinach, green spinach (GS), red spinach (RS), and tree spinach (TS) are consumed in Sri Lanka. GS, RS, and TS are referred to as Basella alba, Basella rubra, and Talinum fruticosum respectively. However, some taxonomists categorize GS and RS under B. alba causing an ambiguity. Due to the poor sanitation, consumers prefer to purchase greenhouse-grown spinach over field grown material. However, the taste parameters of field grown and greenhouse-grown spinach have not been assessed. The objectives of the present study were to resolve the taxonomic ambiguity between GS and RS, identify the evolutionary relationship of TS to other two species and to assess the organoleptic preference on the dishes prepared using greenhouse and field-grown shoot-tops of three spinach. The genomic DNA extracted from GS, RS, and TS, PCR amplified and sequenced for the barcoding markers rbcL, ITS, matK-trnT and atpB-rbcL. The sequences obtained along with other reported related sequences were subjected to phylogenetic analysis. A sensory test was carried out using the shoot-tops of three species grown under greenhouse and field conditions. The taste panelists were asked to rank the dishes for preferred levels of color, aroma, texture, bitterness, and overall taste and the data were subjected to the association analysis. The rbcL and ITS markers separate GS and RS into two well -supported clades, B. alba and B. rubra respectively. The polymorphisms of atpB-rbcL and matK-trnT markers support the definition of two species. The monophyly of B. alba and B. rubra with T. fruticosum must be the reason for the same palate in dishes and designation of all three species under “spinach” in Sri Lanka. The taste panel data demonstrated that there is no specific fondness for greenhouse or field grown materials enabling the popularization of greenhouse-grown spinach to answer the safety concerns.

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Scolopsis igcarensis Mishra, Biswas, Russell, Satpathy & Selvanayagam, 2013, a junior synonym of S. vosmeri (Bloch, 1792) (Perciformes: Nemipteridae)

Zootaxa ◽

10.11646/zootaxa.4629.4.7 ◽

2019 ◽

Vol 4629 (4) ◽

pp. 589-599

Author(s):

BARRY C. RUSSELL ◽

MOHAMMAD EUSUF HASAN ◽

JEAN-DOMINIQUE DURAND

Keyword(s):

Phylogenetic Analysis ◽

Sri Lanka ◽

Coastal Waters ◽

Species Complex ◽

Molecular Data ◽

Junior Synonym ◽

Southern India ◽

Sister Species ◽

Morphological Differences

Scolopsis igcarensis Mishra, Biswas, Russell, Satpathy & Selvanayagam, 2013 was described from specimens collected from coastal waters of southern India and Sri Lanka. A comparison of recently collected specimens from Bangladesh, initially identified as S. igcarensis, with Scolopsis vosmeri (Bloch, 1792) showed morphological differences between the two species are minor, and that specimens of S. igcarensis in fact represent juvenile and subadult colour forms of S. vosmeri. Underwater and aquarium observations, as well as molecular data based on the COI barcode region, support this conclusion. Accordingly, S. igcarensis is regarded as a junior synonym of S. vosmeri, which is redescribed herein. Phylogenetic analysis of COI barcodes of Scolopsis specimens produced in this study, together with those available from GenBank, indicate S. vosmeri is part of a species complex which includes two additional cryptic sister species that require further taxonomic investigation.

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FRUIT MORPHOLOGY OF SECTIONS OF THE GENUS ONOBRYCHIS MILLER (FABACEAE) AND ITS PHYLOGENETIC IMPLICATIONS

Israel Journal of Plant Sciences ◽

10.1080/07929978.1999.10676784 ◽

1999 ◽

Vol 47 (4) ◽

pp. 269-282 ◽

Cited By ~ 18

Author(s):

Bayram Yildiz ◽

Battal Çiplak ◽

Ekrem Aktoklu

Keyword(s):

Phylogenetic Analysis ◽

Taxonomic Position ◽

Fruit Morphology ◽

Phylogenetic Implications

In this study, fruit morphology of sections of the genus Onobrychis were examined. Fruit morphology origin, taxonomic position, and phylogeny of the genus are discussed. The hypothesis obtained from a phylogenetic analysis based on fruit morphology suggests the polyphyly of the genus excluding section Dendrobrychis. However, the result supports monophyly of four generic sections, Hymenobrychis + (Heliobrychis + (Lophobrychis + Onobrychis)).

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The phylogenetic analysis of VP1 genomic region in foot-and-mouth disease virus serotype O isolates in Sri Lanka reveals the existence of 'Srl-97', a newly named endemic lineage

PLoS ONE ◽

10.1371/journal.pone.0194077 ◽

2018 ◽

Vol 13 (3) ◽

pp. e0194077 ◽

Cited By ~ 4

Author(s):

S. A. E. Abeyratne ◽

S. S. C. Amarasekera ◽

L. T. Ranaweera ◽

T. B. Salpadoru ◽

S. M. N. K. Thilakarathne ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Sri Lanka ◽

Disease Virus ◽

Foot And Mouth Disease ◽

Genomic Region ◽

Mouth Disease ◽

Serotype O ◽

Mouth Disease Virus ◽

Virus Serotype ◽

Foot And Mouth

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Revision of the Oriental species of the Bengalia peuhi speciesgroup (Diptera, Calliphoridae)

Zootaxa ◽

10.11646/zootaxa.2251.1.1 ◽

2009 ◽

Vol 2251 (1) ◽

pp. 1-76 ◽

Cited By ~ 2

Author(s):

KNUT ROGNES

Keyword(s):

Phylogenetic Analysis ◽

Sri Lanka ◽

Geographical Distribution ◽

Male Genitalia ◽

Species Group ◽

Junior Synonym ◽

Distributional Range ◽

Colour Photography ◽

Preliminary Phylogenetic Analysis

The Oriental species of the Bengalia peuhi species-group are revised and their male genitalia illustrated by means of digital colour photography. A key to males is presented, complete synonymies are given for all the species and their geographical distribution reconsidered. The 13 Oriental species making up the group are: Bengalia emarginata Malloch, 1927 (China, Laos, Malaysia, Nepal, Philippines, Singapore, Taiwan, Thailand, Vietnam); B. emarginatoides sp. nov. (India, Sri Lanka); B. emdeniella (Lehrer, 2005), comb. nov. (China); B. fani Feng & Wei, 1998 (China); B. inermis Malloch, 1927 (Philippines); B. latro de Meijere, 1910 (Indonesia); B. lyneborgi James, 1966 (Philippines); B. pseudovaricolor Kurahashi & Tumrasvin, 1979 (Thailand); B. surcoufi Senior-White, 1923 (India, Nepal, Pakistan, Vietnam); B. taksina (Lehrer, 2005), comb. nov. (Laos, Malaysia, Nepal, Thailand, Vietnam); B. unicolor Séguy, 1946 (Pakistan); B. varicolor (Fabricius, 1805) (China, India, Indonesia, Japan, Laos, Malaysia, Pakistan, Singapore, Sri Lanka, Taiwan, Thailand, Vietnam); and B. weii sp. nov. (China). A lectotype is designated for Bengalia latro to fix the interpretation of the name. Bengalia bezzii Senior-White, 1923 is established as a junior synonym of Musca varicolor Fabricius, 1805, syn. nov. Bengalia chromatella Séguy, 1946 and Bengalia pallidicoxa Séguy, 1946 are treated as junior synonyms of Musca varicolor Fabricius, 1805, syn. nov. Afridigalia bezziella Lehrer, 2005 is established as a junior synonym of Bengalia emarginata Malloch, 1927, syn. nov. Afridigalia pinatuba Lehrer, 2005 and Ashokiana ramsdalei Lehrer, 2005 are established as junior synonyms of Bengalia inermis Malloch, 1927, syn. nov. Ashokiana ramsdalei and Afridigalia laguna Lehrer, 2005 are both based on a holotype with a partly destroyed aedeagus. Afridigalia tenggeria Lehrer, 2006 is established as a junior synonym of Bengalia latro de Meijere, 1910, syn. nov. Afridigalia thaisia Lehrer, 2008 is established as a junior synonym of Afridigalia taksina Lehrer, 2005, syn. nov. The Afrotropical Afridigalia walkeriana Lehrer, 2005 is established as a junior synonym of Bengalia depressa Walker, 1858, syn. nov. The Afrotropical Afridigalia zouloupyga Lehrer, 2006 is established as a junior synonym of Bengalia floccosa Wulp, 1885, syn. nov. Afridigalia arawakia Lehrer, 2006 is established as a junior synonym of Bengalia peuhi Villeneuve, 1914, syn. nov. Bengalia taiwanensis Fan, 1965, not a member of the peuhi species-group, is established as a junior synonym of Bengalia fuscipennis Bezzi, 1913, syn. nov. Sri Lanka is deleted from the distributional range of this species. New morphological terms are introduced for the description of the aedeagus. The ovipositor is described for Bengalia emarginata and Bengalia lyneborgi. A preliminary phylogenetic analysis is performed using NONA and Pee-Wee.

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