This study is aimed to analyze the use of electronic resources and services in science subjects by the Madras University Library System. Post graduate students, Research scholars, faculties were selected for this study. Questionnaire method was adopted to collect the data. Out of 745 questionnaires 724 (97%) were received from respondents. There are about 161 Educational Institution affiliated to University of Madras of which 15 are Government Colleges, 2 University Constituent Colleges, 24 Aided Colleges, 68 Self Finance Colleges and 52 Research Institutes. Out of these 161 Educational Institutions 136 offer Science courses. Apart from this the university itself has 31 departments catering to Science subjects spread over 3 different campuses. Altogether 167 Institutions, affiliated to University of Madras, are offering courses in science subjects. Out of these, 31 University departments have been selected for the study.