scholarly journals Extraction and characterization of bio-based polymeric materials from traditional rice varieties in Sri Lanka

2018 ◽  
Vol 30 (2) ◽  
pp. 47
Author(s):  
W. G. A. Pabasara ◽  
M. P. A. Nanayakkara ◽  
A. M. P. B. Samarasekara ◽  
D. A. S. Amarasinghe ◽  
L. Karunanayake
Keyword(s):  
Sri Lanka ◽  
Polymeric Materials ◽  
Rice Varieties ◽  
Traditional Rice

Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Shyama Weerakoon ◽  
Seneviratne Somaratne
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Sri Lanka ◽  
Core Collection ◽  
Morphological Characters ◽  
Morphological Features ◽  
Sri Lankan ◽  
Rice Varieties ◽  
Traditional Rice ◽  
Phenotypic And Genetic Diversity

Abstract. Weerakoon SR, Somaratne S. 2021. Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity. Nusantara Bioscience 13: 61-67. A collection of over 2000 traditional rice varieties are conserved at Gene Bank, Plant Genetics Resource Center, Sri Lanka. Oryza sativa varieties grown in Sri Lanka from ancient times to the middle of the last century are known as traditional rice. These varieties show adaptability to biotic and abiotic stresses and, an important component of biodiversity of Sri Lanka. A detailed understanding of the diversity of traditional rice varieties is essential for effective utilization of rice genetic resources and identification of potential parents possessing valuable genetic traits for future crop improvement. Study objectives were phenotypic and molecular characterization of one-hundred traditional rice varieties and to identify a core collection for phenotypic and genetic diversity. Rice varieties were grown in a plant house following RCBD with 4 replicates and 5 plants per replicate. Thirty-two agro-morphological characters were observed/collected. Genomic DNA was extracted from 20-days-old seedlings. Thirty?three microsatellite (Simple Sequence Repeat-SSR) primer pairs were used to assay genetic variation and PCR products were subjected to fragment analysis by capillary electrophoresis. Descriptive statistics and basic inferential statistical analyses were performed to access variation of agro-morphological characters among rice varieties. Cluster analysis and Multidimensional scaling produced 07 groups which were further analyzed using Classification and Regression Analysis to extract the diagnostic agro-morphological features. Groups of rice varieties were characterized by lemma palea color, awn color at maturity, seedling height, and flag-leaf angle. Traditional varieties represent distant clusters on agro-morphological features. Molecular analyses revealed all 33 loci displayed polymorphism (66.7-96.9%) among 100 traditional rice varieties with a total of 387 alleles identified with an average of 11.72 alleles per variety. All varieties were genetically structured into fifteen well-separated groups. UPGMA analysis based on Jaccard's similarity separated varieties into 05 major clusters. Genetic diversity information is useful in the efficient use of Sri Lankan rice germplasm and managing in situ and ex situ germplasm collections in conserving traditional rice varieties.

scholarly journals Physical and nutrient characterization of the grains from traditional rice varieties of Kerala and validation of the trait linked molecular markers

2021 ◽  
Vol 58 (3) ◽  
pp. 394-408
Author(s):  
Jeena George ◽  
VG Jayalekshmy ◽  
AM Shahiba ◽  
Arun Chacko ◽  
Tejashree S Lachyan
Keyword(s):  
Protein Content ◽  
Cooking Quality ◽  
Amylose Content ◽  
Public Awareness ◽  
Zinc Content ◽  
Rice Varieties ◽  
Carotene Content ◽  
Iron And Zinc ◽  
Traditional Rice

Kerala is rich with traditional rice varieties that could grow in various agro- climatic conditions of the state. However many of these traditional varieties are not cultivated and have become extinct due to low yield, promotion of high breed variety seeds and high-cost in continuing the cultivation with traditional seeds. The lack of knowledge about the nutraceutical values of traditional rice varieties among farmers leads to the non-participation in cultivation. Present study was aimed at to characterization of thirty traditional rice varieties in terms of physico-chemical, cooking quality as well as molecular screening with markers specific to quality traits. All the varieties used is unique in their quality parameters, among them Njavara Veluthath (13.57%) and Orumundakan (13.35%) were rich in protein content whereas, Karimbalan (311.54 mg/Kg and 102.41 mg/kg) and Veliyan (218.25 mg/Kg and 56.88mg/kg) were superior in Iron and Zinc content. The carotene content ranged from 0.14 mg/100g (Mullan kayama) to 0.56 mg/100g (Njavara veluthath) and amylose content was maximum in Cheruviripp (29.52%). Thus, these varieties need to be conserved and promote them by commercialize and through general public awareness about their medicinal benefits. Correlation analysis showed positive significant relationship between Iron and Zinc content, while amylose content had positive correlation with volume expansion and optimum cooking quality. The SSR marker RM535 linked to Quantitative Trait Loci for Iron and Zinc content was detected in thirteen varieties, RM190 linked with Wx gene locus associated with amylose content in seventeen varieties and the marker RM520 linked to QTL for protein content was detected in all the varieties.

scholarly journals Macronutrient and Mineral Composition of Selected Traditional Rice Varieties in Sri Lanka

2019 ◽  
Author(s):  
Amali Kulasinghe ◽  
◽  
Gamini Samarasinghe ◽  
Swarna Wimalasiri ◽  
Renuka Silva ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Mineral Composition ◽  
Rice Varieties ◽  
Traditional Rice

Storage and hydro-priming treatments to improve the seed quality of two traditional rice varieties; Batapola-el and Suwendal, from Sri Lanka

2020 ◽  
Vol 57 (1) ◽  
pp. 25-35
Author(s):  
MO Galappaththi ◽  
KMGG Jayasuriya ◽  
NS Gama-Arachchige
Keyword(s):  
Sri Lanka ◽  
Seed Germination ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Seed Viability ◽  
Rice Varieties ◽  
Seed Vigour ◽  
Sowing Season ◽  
Traditional Rice

Although the demand for traditional rice varieties has increased, low initial germination and poor storability of these varieties impede its popularization. Thus, the aim of the study was to improve the seed quality using priming treatments and to suggest an effective storage practice of two of the traditional rice varieties; Suwendal and Batapola-el, in Sri Lanka. Seeds were subjected to hydro-priming under various time periods (0, 24, 48 and 72 hours). Seeds were stored in gunny, polysac and polythene bags at room temperature, 25 and 8 °C. Seed quality of primed and stored seeds was evaluated using seed germination, seed vigour (seedling growth and conductivity) and seedling emergence. Hydro-priming for 72 hours has increased the seed germination of the two varieties significantly compared to other priming methods. Further, seeds primed for 72 hours had the highest vigour and highest seedling emergence in both varieties. Storing Suwendal and Batapola-el seeds in polythene bags at 8 °C retained the highest viability and highest vigour for more than six months. Thus, hydro-priming for 72 hours could be recommended to enhancethe seed quality of these two tested varieties and storage at 8 °C in sealed polythene bags could be recommended as an effective storage practice to maintain the seed viability till the next sowing season.

scholarly journals Development of a Diversity Panel for Traditional Rice (Oryza Sativa L.)Accessions/Varieties in Sri Lanka

2020 ◽  
Vol 03 (12) ◽  
Author(s):  
S. Somaratne ◽  
Keyword(s):  
Oryza Sativa ◽  
Sri Lanka ◽  
Oryza Sativa L ◽  
Morphological Characters ◽  
Morphological Features ◽  
Ex Situ ◽  
Rice Varieties ◽  
Diversity Panel ◽  
Breeding Programmes ◽  
Traditional Rice

Oryza sativa L. varieties grown in Sri Lanka from ancient times to the middle of the last century are known as traditional rice varieties and a collection of over 2000 traditional rice accessions conserved at Gene Bank, Plant Genetics Resource Center (PGRC), Peradeniya, Sri Lanka. Farmers preferred traditional rice varieties for their adaptability to biotic and abiotic stresses and are an important component of the biodiversity of Sri Lanka. A detailed understanding of the genetic structure and diversity of traditional rice varieties is essential for the effective utilization of rice genetic resources and identification of potential parents possessing valuable genetic traits for future crop improvement in rice breeding programmes. The objective of the present study was phenotypic and molecular characterization of one hundred (100) traditional rice accession/varieties collected from PGRC, Sri Lanka and identification of a broad diversity panel for these traditional rice accessions/varieties. Rice varieties were grown in a plant house following Randomized Complete Block design with 4 replicates and 5 plants per each replicate. Thirty-two (32) agro-morphological characters were observed. Green leaves of rice varieties were individually collected from 20 day-old seedlings for gDNA extraction using Plant genomic DNA kit followed by the CTAB protocol. Thirty‐three microsatellite (Simple Sequence Repeat – SSR) primer pairs were used to assay genetic variation. DNA amplification was carried out using a thermal cycler and PCR products were subjected to fragment analysis by capillary electrophoresis. Descriptive statistics and basic inferential statistical analyses were performed to access the variation of agro-morphological characters among rice varieties. Data were subjected to cluster analysis (CA) to examine the grouping tendencies and supplemented with Multidimensional scaling (MDS) to explore the procedural differences in the outcome. CA and MDS produced seven (07) groups which were further analyzed using Classification and Regression Analysis (CART) to extract the diagnostic agro-morphological features. Based on CART result, groups of rice varieties were characterized by lemma–palea color, presence or absence of awn, seedling height, and flag-leaf angle. Traditional rice accessions/varieties represent distant clusters on agro-morphological features. Molecular analyses revealed, all 33 loci displayed polymorphism (66.7-96.9 %) among 100 traditional rice accessions/varieties with a total of 387 alleles identified with an average of 11.72 alleles per accession. AMOVA results showed that 34% of the variation distributed among accessions/varieties, 59% of among individuals and 7% within individual indicating a comparatively high level of genetic differentiation among individuals of selected rice accessions/varieties. Structure analysis results illustrated that all 100 accessions/varieties were genetically structured into fifteen well-separated groups, high ΔK peak was recorded at K=15, K= 5, K= 19 and K= 2 respectively. UPGMA analysis based on Jaccard's similarity separated the accessions into five (5) major clusters. A cophenetic correlation with r=0.786 strongly supported the clustering pattern of UPGMA dendrogram. A principal coordinate analysis (PCoA) also confirmed the UPGMA clusters. The genetic diversity information obtained will be useful in efficient use of Sri Lankan rice germplasm collection in breeding programmes. This information will also be useful in management of in situ and ex situ germplasm collections in conservation programs for traditional rice varieties.

scholarly journals Identification of fragrant gene, fgr, in traditional rice varieties of Sri Lanka

2010 ◽  
Vol 38 (2) ◽  
pp. 139 ◽  
Author(s):  
N.S. Kottearachchi ◽  
E.G.D. Priyangani ◽  
D.P.S.T.G. Attanayaka
Keyword(s):  
Sri Lanka ◽  
Rice Varieties ◽  
Traditional Rice
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