scholarly journals Genotype Affected Callus Induction and Plant Regeneration from Dehusked Rice (Oryza sativa L.) Seeds

2010 ◽  
pp. 44-49
Author(s):  
MY Miah ◽  
ME Hoque ◽  
MM Islam ◽  
M Ahmed ◽  
Kazi Noor-E-Alam Jewel
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Regeneration Ability ◽  
Rice Cultivars ◽  
South West ◽  
Randomized Design ◽  
Murashige Skoog ◽  
Rice Genotypes ◽  
Varietal Improvement

Callus induction and plant regeneration ability of the dehusked rice genotypes are important tools for varietal improvement. This investigation presents the comparative callus induction and plant regeneration as obtained from the dehusked seeds of rice cultured on MS (Murashige-Skoog) medium. In the experiment conducted in a Complete Randomized Design under laboratory conditions, dehusked seeds of Ranisalute, Chiniatap, Somudrafena, Chiniguri-2 and Katchra rice cultivars grown in South West Bangladesh were tested on autoclaved and agarified MS nutrient medium with four replications to study their callus induction and subsequent plant regeneration ability. Chiniguri-2 yielded the highest calli and plant regeneration.

scholarly journals Regenerasi dan Aklimatisasi Kultur Antera Enam Persilangan F1 Padi Sawah

2016 ◽  
Vol 44 (2) ◽  
pp. 133
Author(s):  
Cucu Gunarsih ◽  
Bambang Sapta Purwoko ◽  
Iswari Saraswati Dewi ◽  
Dan Muhamad Syukur
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Callus Induction ◽  
Rainfed Rice ◽  
Randomized Design ◽  
Doubled Haploid Plants ◽  
Completely Randomized Design ◽  
N6 Medium ◽  
Haploid Plants

ABSTRACT<br /><br />The breeding of rainfed rice tolerant to drought can be accomplished using anther culture. The objectives of this research were to determine regeneration abilities of six F1 anther culture and its acclimatization ability. The experiment was arranged in completely randomized design with 14 replications. The treatments consisted of six F1 derived from crossing:  INPARI 18 x IR83140-B-11-B (G1), INPARI 18 x B12825E-TB-1-25 (G2), INPARI 18 x IR87705-14-11-B-SKI-12 (G3), INPARI 22 x IR83140-B-11-B (G4), Bio-R81 x O18b-1 (G5), Bio-R82-2 x O18b-1 (G6). Media for callus induction was based on N6 medium + 2.0 mg L-1 NAA + 0.5 mg L-1 kinetin + 1.0 mM putresin + 60 g L-1 sucrosa, media for regeneration was based on MS + 0.5 mg L-1 NAA + 2.0 mg L-1 kinetin + 1.0 mM  putresin, and media for rooting was based on  MS + 0.5 mg L-1 IBA + 30 g L-1 sucrosa. The result indicated that all six F1 had different ability in anther culture. Bio-R82-2 x O18-b1 (G6) and  Bio-R81 x O18-b1 (G5) F1 genotype had good response both of callus induction and plant regeneration. These two F1 genotypes also gave the highest ratio of green planlet production to number of anther inoculated (GP:AI) were 5.50% and 4.65%,  respectively. In this research, there were identified doubled haploid plants were developed from 4 F1 derived cross namely G2 (2 plants), G3 (4 plants),  G5 (21 plants), and G6 (26 plants).<br /><br />Keywords: Callus induction, doubled haploid, rice<br /><br />

Callus induction and plant regeneration of U.S. rice genotypes as affected by medium constituents

1996 ◽  
Vol 32 (4) ◽  
pp. 227-232 ◽  
Author(s):  
Jameel M. Al-Khayri ◽  
Christine E. Shamblin ◽  
Edwin J. Anderson
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Rice Genotypes

scholarly journals Screening of Rice (Oryza sativa L.) Genotypes at Reproductive Stage for their Tolerance to Salinity

2018 ◽  
Vol 16 (1) ◽  
pp. 65-77
Author(s):  
M M Islam ◽  
M H Faruqe ◽  
M S Rana ◽  
M Akter ◽  
M A Karim
Keyword(s):  
Oryza Sativa ◽  
Salt Tolerance ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Saline Water ◽  
Coastal Zones ◽  
Salt Tolerant ◽  
Randomized Design ◽  
Rice Genotypes ◽  
Four Levels

Soil salinity is one of the most devastating environmental stresses for rice production in the coastal areas of Bangladesh. Improvement in salt tolerance of rice is an important way for the economic utilization of coastal zones. An experiment was conducted at the vinyl house of the Department of Agronomy, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Bangladesh during December 2016 to April 2017 to determine the effects of different salinity levels on the yield and yield components of some rice (Oryza sativa L.) genotype sand finally, screening of rice genotypes for salt tolerance. The experiment containing four treatments was laid out in a complete randomized design with five replications. The treatments were four levels of saline water with electrical conductivities at control (0.3 dSm-1), 5, 10 and 15dSm-1. Based on screening at germination stage, relatively salt-tolerant eleven genotypes with two check varieties were used in this experiment. The study showed that increase in salinity level significantly decreased yield and yield contributing characters of rice. However, among the eleven, three genotypes (Chapali, Patnai 23 and Soloi) were considered as moderately salt tolerant at 15 dSm-1 on the basis of their yields and yield contributing characteristics such as plant height reduction, total tiller reduction, effective tiller reduction, reduction of fertile grains per panicle, grain yield and relative grain yield. Therefore, Chapali, Patnai 23 and Soloi might be recommended as moderately salt tolerant rice genotypes. The Agriculturists 2018; 16(1) 65-77

scholarly journals Authentication, genetic fingerprinting and assessing relatedness of rice (Oryza Sativa) genotypes by SSR molecular markers

Caryologia ◽  
2021 ◽  
Vol 74 (1) ◽  
pp. 13-22
Author(s):  
Neda Sepahian ◽  
Zahra Noourmohammadi ◽  
Masoud Sheidai ◽  
Hamid-Reza Zamanizadeh
Keyword(s):  
Oryza Sativa ◽  
Molecular Markers ◽  
Genetic Relatedness ◽  
Isolation By Distance ◽  
Genetic Difference ◽  
Rice Cultivars ◽  
Geographical Regions ◽  
Ssr Loci ◽  
Rice Samples ◽  
Rice Genotypes

Rice (Oryza sativa L.), is a staple food and cash crop in many countries and studies on geneticstructure and differentiation patterns of rice land races along with the cultivated rice, provide important data for future rice breeding. Therefore, the aims of present investigation were 1-To study the genetic diversity present withinIranian rice genotypes, 2-To study genetic relatedness of these rice genotypes, and 3-To providebarcoding of the rice genotypes based on SSR molecular markers and produce data for rice varieties authentication. In total, 201 rice samples originated from 10 geographical regions of Iran were studied in this project. All rice samples underwent fragment analysis in every 64 SSR loci and different clustering and ordination methods performed. In general four major clusters were formed. Both landraces as well as rice cultivars were distributed in different clusters due to their genetic difference. STRUCTURE analysis of the studied genotypes followed by Evanno test produced the optimal number of genetic groups K = 2. The mean Nm = 13.6, for the studied genotypes indicates that a high degree of gene flow/ancestral common alleles are present in the rice genotypes studied. Mantel test indicated a significant positive association between genetic distance and geographic distance of the rice genotype studied and presence of an overall isolation by distance (IBD) model of differentiation across the geographical regions of Iran. Overall, the significant genetic difference observed between rice landraces and rice cultivars ofthe country may be used in future hybridization and breeding of rice in the country. The landracerice genotypes may contain useful genes to be transferred to the popular rice cultivars. Moreover, SSR loci that can differentiate rice genotypes are identified and can be used in rice cultivars authentication.

scholarly journals Optimization for Callus Induction and Plant Regeneration from Mature Seeds of Thai Rice Variety: Nam Roo (Oryza sativa L.)

2016 ◽  
Vol 4 (5) ◽  
pp. 95-99
Author(s):  
Anurug Poeaim ◽  
Supattra Poeaim ◽  
Ranyikar Poraha ◽  
Saengthong Pongjaroenkit ◽  
Pradit Pongthongkam
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Mature Seeds
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