scholarly journals Yeast Extract Promotes Cell Growth and Induces Production of Polyvinyl Alcohol-Degrading Enzymes

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Min Li ◽  
Xianyan Liao ◽  
Dongxu Zhang ◽  
Guocheng Du ◽  
Jian Chen
Keyword(s):  
Amino Acids ◽  
Cell Growth ◽  
Polyvinyl Alcohol ◽  
Yeast Extract ◽  
Low Energy ◽  
Degrading Enzymes ◽  
Dry Cell Weight ◽  
Low Energy Consumption ◽  
New Strategy ◽  
Dry Cell

Polyvinyl alcohol-degrading enzymes (PVAases) have a great potential in bio-desizing processes for its low environmental impact and low energy consumption. In this study, the effect of yeast extract on PVAases production was investigated. A strategy of four-point yeast extract addition was developed and applied to maximize cell growth and PVAases production. As a result, the maximum dry cell weight achieved was 1.48 g/L and the corresponding PVAases activity was 2.99 U/mL, which are 46.5% and 176.8% higher than the control, respectively. Applying this strategy in a 7 L fermentor increased PVAases activity to 3.41 U/mL. Three amino acids (glycine, serine, and tyrosine) in yeast extract play a central role in the production of PVAases. These results suggest that the new strategy of four-point yeast extract addition could benefit PVAases production.

Effects of Yeast Extract on Poly-ε-Lysine Production in Batch Culture of Streptomyces Albulus

2011 ◽  
Vol 343-344 ◽  
pp. 1023-1028 ◽  
Author(s):  
Xiong Chen ◽  
Zhi Wang ◽  
Yong Ze Wang ◽  
Fa Tang Jiang ◽  
Dong Sheng Li ◽  
...  
Keyword(s):  
Nitrogen Content ◽  
Yeast Extract ◽  
Organic Nitrogen ◽  
Pantothenic Acid ◽  
Amino Nitrogen ◽  
Lysine Production ◽  
Dry Cell Weight ◽  
Brewers Yeast ◽  
Organic Nitrogen Source ◽  
Dry Cell

Yeast extract (YE) is a sole organic nitrogen source in a commonly medium 3G for Streptomyces albulus to produce poly-ε-lysine (ε-PL). In this study, five YEs from five commercial suppliers were used to evaluate their effects on the growth of Streptomyces albulus 213 and ε-PL production. YE from bakers’ yeast with the highest total nitrogen content (TN), α-amino nitrogen content (AN) or the ratio of AN/TN produced the highest yield of ε-PL, while YE from brewers’ yeast with the highest RNA content got the most dry cell weight (DCW). However, there was little correlation between TN, AN or AN/TN in YE and the yield of ε-PL. There was also weak correlation between RNA in YE and DCW. When 60% baker YE was mixed with 40% brewer YE, the yield of ε-PL reached 1.89 g/l and increased by 16% and 37% than those of baker YE and brewer YE, respectively. Furthermore, five kinds of vitamins and nine kinds of amino acids were screened to supplement baker YE. When 6 mg/l biotin, 5mg/l pantothenic acid, 0.7 g/l L-glutamine and 0.5 g/l L-arginine were mixed with baker YE in M3G, ε-PL reached maximum production respondent to 3.05 g/l that was 61.4% higher than control.

scholarly journals Effect of Carbon Sources, Nitrogen Sources and Prebiotics on Growth of Saccharomyces Boulardii

2019 ◽  
Vol 23 (2) ◽  
pp. 101-108
Author(s):  
Xin Yang ◽  
Guowei Shu ◽  
Zhangteng Lei ◽  
Guanli Du ◽  
Zemin Liu ◽  
...  
Keyword(s):  
Yeast Extract ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Gastrointestinal Diseases ◽  
Potassium Nitrate ◽  
Soluble Starch ◽  
Saccharomyces Boulardii ◽  
Dry Cell Weight ◽  
Wide Range ◽  
Dry Cell

Abstract Saccharomyces boulardii (S. boulardii) has been used as a probiotic for the prevention or treatment of various human gastrointestinal diseases for many years. Thus, S. boulardii has a wide range of application prospects in medicine and food industry. The experiments were investigated with effecting of carbon sources (galactose, sucrose, fructose, maltose, lactose, glucose, and soluble starch), nitrogen sources (tryptone, casein, yeast extract, peptone, soy peptone, beef extract and malted milk; ammonium sulfate, urea, diammonium hydrogen citrate, triammonium citrate, ammonium nitrate, ammonium chloride and potassium nitrate), prebiotics (xylo-oligosaccharide, isomaltooligosaccharide, fructo-oligosaccharide, galacto-oligosaccharide, stachyose, raffinose and inulin) on the number of viable cells and dry cell weight of S. boulardii. The optimum concentration of 5 % glucose or sucrose, 2 % peptone or yeast extract, 0.4 % urea, 0.2 % fructo-oligosaccharide and 0.6 % galacto-oligosaccharide for S. boulardii grew better in medium.

scholarly journals Overexpression of Shinorhizobium Meliloti Flavohemoglobinefficiently Improved Cell Growth and Fatty Acid Biosynthesis in Oleaginous Fungus Mucor Circinelloides

2021 ◽  
Author(s):  
Huaiyuan Zhang ◽  
Xinxin Kang ◽  
Ruixue Wang ◽  
Feifei Xin ◽  
Yufei Chang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Cell Growth ◽  
Acid Production ◽  
Difference Spectrum ◽  
Mucor Circinelloides ◽  
Fatty Acid Production ◽  
Cell Weight ◽  
Dry Cell Weight ◽  
Eukaryotic Microorganisms ◽  
Dry Cell

Abstract Oxygen availability is a limiting factor for lipid biosynthesis in eukaryotic microorganisms. Two bacterial hemoglobins from Vitreoscilla sp. (VHb) and Shinorhizobium meliloti (SHb), which could deliver the oxygen to the respiratory chain to produce more ATP, were introduced into Mucor circinelloides to alleviate oxygen limitation, thereby improving cell growth and fatty acid production. VHb and SHb genes were integrated into the M. circinelloides MU402 genome through homologous recombination, and their protein expression was verified by carbon monoxide difference spectrum (CO-difference spectrum)analysis. SHb-expressing strain showed higher biomass than VHb-expressing strain. The biomass of the SHb-expressing strain was increased by about 50% and the total fatty acid (TFA) content was as high as 15.7% of the dry cell weight which was about 40% higher than that of the control strain in flask conditions. In the fermenter, the maximum biomass and TFA content was obtained in SHb-expressing strains, with the biomass being 12.1 g/L and the TFA being 21.1% of the dry cell weight. VHb and SHb expression also affected the fatty acid composition with the proportion of polyunsaturated fatty acids being increased. Over-expression of bacterial hemoglobins, especially SHb increased cell growth and TFA content in M. circinelloides at low and high aeration, suggesting that SHb is better than VHb in improving the fatty acid production in oleaginous microorganisms.

Influence of Initial pH and Temperature on Fermentative Biohydrogen Production of Biohydrogenbacterium R3 sp.nov. from Glucose

2011 ◽  
Vol 71-78 ◽  
pp. 2929-2932 ◽  
Author(s):  
Yong Feng Li ◽  
Hong Chen ◽  
Wei Han ◽  
Fang Jing Liu ◽  
Zhan Qing Wang
Keyword(s):  
Cell Growth ◽  
Hydrogen Production ◽  
Glucose Concentration ◽  
Ph Value ◽  
Initial Glucose Concentration ◽  
Noticeable Effect ◽  
Dry Cell Weight ◽  
Initial Ph ◽  
Maximum Cell ◽  
Dry Cell

This study investigated the effects of initial pHs and temperatures to the hydrogen production ration and cell growth ofBiohydrogenbacteriumR3sp.nov.. The initial pHs were set at 4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 6.7, respectively and the temperatures were increased from 25 °C to 45 °C in regular intervals of 5 °C at 10 g/L of the initial glucose concentration. The results indicated that pH value had a noticeable effect on the cell growth and hydrogen production. The dry cell weight and hydrogen production yield got the maximum of 0.6308 g/L and 34.2 mmol/L, respectively when the initial pH was 5.5. The final pH in the culture were always kept at 3.0~4.0. Temperature is also known to affect the maximum cell growth and specific hydrogen production ration (SHPR). And they got the maximum of 0.6682 g/L and 1.0145mol H2/mol glucose, respectively when the temperature was 30 °C. It is obvious that hydrogen production and biomass will be inhibited gradually with increasing this pH and temperature or decreasing it.

A Study on the Use of Bipolar-Particles-Electrodes in Decolorization of Dyeing Effluents and Its Principle

1987 ◽  
Vol 19 (3-4) ◽  
pp. 391-400 ◽  
Author(s):  
Zhou Ding ◽  
Cai Wei Min ◽  
Wang Qun Hui
Keyword(s):  
Energy Consumption ◽  
High Efficiency ◽  
Low Energy ◽  
Acid Dyes ◽  
Underlying Principle ◽  
Low Energy Consumption ◽  
Transparent Liquid

This paper studies the use of bipolar-particles-electrodes in the decolorization of dyeing effluents. Treatment of highly colored solutions of various soluble dyes (such as direct, reactive, cationic or acid dyes) and also samples of dyeing effluents gave rise to an almost colorless transparent liquid, with removal of CODcr and BOD5 being as high as over 80%. The method is characterized by its high efficiency, low energy consumption and long performance life. A discussion of the underlying principle is given.

Bubble Electrospinning and Bubble-Spun Nanofibers

2020 ◽  
Vol 14 (1) ◽  
pp. 10-13 ◽  
Author(s):  
Lynn Y. Wan
Keyword(s):  
Surface Tension ◽  
Low Energy ◽  
Efficient Technology ◽  
High Productivity ◽  
Needleless Electrospinning ◽  
Polymeric Nanofibers ◽  
Low Energy Consumption ◽  
Potential Applications ◽  
Surface Morphologies ◽  
Unique Surface

Electrospinning is a highly efficient technology for fabrication of a wide variety of polymeric nanofibers. However, the development of traditional needle-based electrospinning has been hampered by its low productivity and need of tedious work dealing with needles cleaning, installation and uninstallation. As one of the most promising needleless electrospinning means, bubble electrospinning is known for its advantages of high productivity and relatively low energy consumption due to the introduction of a third force, air flow, as a major force overcoming the surface tension. In this paper, the restrictions of conventional electrospinning and the advantages of needleless electrospinning, especially the bubble electrospinning were elaborated. Reports and patents on bubble-spun nanofibers with unique surface morphologies were also reviewed in respect of their potential applications.

The influence of the initial concentrations of glucose and yeast extract on the ethanolic fermentation by Pachysolen tannophilus

1990 ◽  
Vol 55 (3) ◽  
pp. 854-866 ◽  
Author(s):  
Rodríguez V. Bravo ◽  
Rubio F. Camacho ◽  
Villasclaras S. Sánchez ◽  
Vico M. Castro
Keyword(s):  
Cell Growth ◽  
Ethanol Production ◽  
Yeast Extract ◽  
Culture Medium ◽  
Ethanolic Fermentation ◽  
Pachysolen Tannophilus ◽  
Significant Component ◽  
Batch Cultures

The ethanolic fermentation in batch cultures of Pachysolen tannophilus was studied experimentally varying the initial concentrations of two of the components in the culture medium: glucose between 0 and 200 g l-1 and yeast extract between 0 and 8 g l-1. The yeast extract appears to be a significant component both in cell growth and for ethanol production.

Top-down Fabricated Gold Nanostrip on Silicon-on-insulator Wafer: A Promising Building Block towards Ultra-compact Optical Device

Nanoscale ◽  
2020 ◽  
Author(s):  
Fuping Zhang ◽  
Weikang Liu ◽  
Li Chen ◽  
Zhiqiang Guan ◽  
Hongxing Xu
Keyword(s):  
Optical Communication ◽  
Data Transmission ◽  
Building Block ◽  
High Speed ◽  
Large Data ◽  
Low Energy ◽  
Silicon On Insulator ◽  
Fundamental Building Block ◽  
Low Energy Consumption ◽  
Controllable Fabrication

he plasmonic waveguide is the fundamental building block for high speed, large data transmission capacity, low energy consumption optical communication and sensing. Controllable fabrication and simultaneously optimization of the propagation...

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