Production and Optimization of Cellulase Activity of Thermomonospora Viridis Isolated From Rice Straw

2021 ◽  
Vol 50 (2) ◽  
pp. 395-404
Author(s):  
Faozia Faleha Sadida ◽  
Ma Manchur
Keyword(s):  
Enzyme Activity ◽  
Metal Ions ◽  
Rice Straw ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Tetraacetic Acid ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Optimum Ph

A highly cellulolytic actinomycete SR1 was locally isolated from rice straw and provisionally identified as Thermomonospora viridis. Optimum pH, temperature, carbon and nitrogen sources for its cellulase production were 6.5, 35°C, Carboxymethyl cellulase (CMC) and yeast extract, respectively whereas those of cellulase activity were 7.5, 40°C, CMC and peptone respectively. The effects of various metal ions and different reductant and inhibitors on its cellulase activity were investigated. Univalent Ag+ was found to decrease the enzyme activity whereas increased by bivalent Mg2+. Ethylene diamine tetraacetic acid (EDTA) caused remarkable decrease of cellulase activity but β-Mercaptoethanol stimulated its cellulase activity. Bangladesh J. Bot. 50(2): 395-404, 2021 (June)

scholarly journals Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
S. P. Gautam ◽  
P. S. Bundela ◽  
A. K. Pandey ◽  
Jamaluddin Khan ◽  
M. K. Awasthi ◽  
...  
Keyword(s):  
Municipal Solid Waste ◽  
Solid Waste ◽  
Enzyme Production ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Production Medium ◽  
Optimum Temperature ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Alternative Carbon Source

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.

scholarly journals Production of Xylanase from Arthrobacter sp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Sevanan Murugan ◽  
Donna Arnold ◽  
Uma Devi Pongiya ◽  
P.M. Narayanan
Keyword(s):  
Enzyme Activity ◽  
Solid State ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Optimal Temperature ◽  
Carbon And Nitrogen Sources ◽  
Xylanase Production ◽  
Carbon And Nitrogen ◽  
Saw Dust ◽  
Production Temperature

Saw dust was used as substrate for xylanase production from Arthrobacter sp. MTCC 6915. The study of period of incubation, temperature, pH, carbon, and nitrogen sources for xylanase production was optimized. Xylanase production was found to be optimum at an incubation period of 96 hrs (117.0 U/mL), temperature 30°C (105.0 U/mL), and pH 9.0 (102.9 U/mL). The results showed that the xylanase production was found to be higher in the presence of carboxymethylcellulose (176.4 U/mL) and dextrose (126.0 U/mL). It was also observed that peptone (170.1 U/mL) and beef extract (161.7 U/mL) supported maximum xylanase production.The enzyme was characterized and found to be fairly active at pH 9 (764.4 U/mL) and temperature 60°C (819 U/mL). Even in the present study, a major difference in the production temperature (30°C) and optimal temperature (60°C) of the enzyme activity was observed. However, the pH of the production media and the enzyme activity were found to be the same (pH 9).

Influences of carbon and nitrogen sources and metal ions on the heterotrophic culture of Scenedesmus sp. LX1

2019 ◽  
Vol 26 (13) ◽  
pp. 13381-13389 ◽  
Author(s):  
Yitian He ◽  
Yu Hong ◽  
Xiaoya Liu ◽  
Qing Zhang ◽  
Peirui Liu ◽  
...  
Keyword(s):  
Metal Ions ◽  
Nitrogen Sources ◽  
Heterotrophic Culture ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

scholarly journals The GATA-Type Transcriptional Factor Are1 Modulates the Expression of Extracellular Proteases and Cellulases in Trichoderma reesei

2019 ◽  
Vol 20 (17) ◽  
pp. 4100 ◽  
Author(s):  
Yuanchao Qian ◽  
Yu Sun ◽  
Lixia Zhong ◽  
Ningning Sun ◽  
Yifan Sheng ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Pcr Analysis ◽  
Transcription Activator ◽  
Extracellular Proteases ◽  
Promoter Regions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Significant Difference

Trichoderma reesei is a biotechnologically important filamentous fungus with the remarkable ability to secrete large amounts of enzymes, whose production is strongly affected by both the carbon and nitrogen sources. While the carbon metabolism regulators are extensively studied, the regulation of enzyme production by the nitrogen metabolism regulators is still poorly understood. In this study, the GATA transcription factor Are1, which is an orthologue of the Aspergillus global nitrogen regulator AREA, was identified and characterized for its functions in regulation of both protease and cellulase production in T. reesei. Deletion of the are1 gene abolished the capability to secrete proteases, and complementation of the are1 gene rescued the ability to produce proteases. Quantitative RT-PCR analysis revealed that the transcripts of protease genes apw1 and apw2 were also significantly reduced in the Δare1 strain when grown in the medium with peptone as the nitrogen source. In addition, deletion of are1 resulted in decreased cellulase production in the presence of (NH4)2SO4. Consistent with the reduction of cellulase production, the transcription levels of the major cellulase genes, including cbh1, cbh2, egl1, and egl2, were dramatically decreased in Δare1. Sequence analysis showed that all promoter regions of the tested protease and cellulase genes contain the consensus GATA elements. However, the expression levels of the major cellulase transcription activator Xyr1 and the repressor Cre1 had no significant difference between Δare1 and the parental strain QM9414, indicating that the regulatory mechanism deserves further investigation. Taken together, these results demonstrate the important role of Are1 in the regulation of protease and cellulase production in T. reesei, although these processes depend on the kind of nitrogen sources. The findings in this study contribute to the understanding of the regulation network of carbon and nitrogen sources in filamentous fungi.

scholarly journals Semi-quantitative detection for L-asparaginase producing fungi and the impact of carbon and nitrogen sources on enzyme activity

2021 ◽  
Author(s):  
Mahdi Hamed ◽  
Ahmed A Osman ◽  
Mustafa Ateş
Keyword(s):  
Enzyme Activity ◽  
Enzyme Production ◽  
Enzyme Level ◽  
Nitrogen Sources ◽  
Quantitative Detection ◽  
Phenol Red ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Fungal Isolates ◽  
The Impact

AbstractObjectiveTo semi-quantitively screen filamentous fungi isolated from different habitats for L-asparaginase production by three indicators; phenol red, cresol red and bromothymol blue and to examine the impact of different carbon and nitrogen sources on the enzyme production using different fungal isolates.Materials and methodsFifty-five fungal isolates were tested for L-asparaginase production by plate assay using Modified Czapek-Dox (MCD) medium. The enzyme activity was estimated using the Nessler method which measures the concentration of ammonia formed owing to the enzyme action on the substrate. The impact of nitrogen and carbon sources on the enzyme production was done by using the best three L-asparaginase producers from the semi-quantitative screening.Results and conclusionsA total of 53/55 (96.36%) fungal isolates were L-asparaginase producing strains, of them, Cladosporium tenuissimum, Penicillium camembertii and Aspergillus carneus showed high enzyme production. Production of L-asparaginase was higher with the glucose and urea as carbon and nitrogen sources, respectively. The highest enzyme level (5,558 U/ml) was produced by C. tenuissimum in a glucose-containing medium. This study shows that P. camemberti, A. carneus, and C. tenuissimum are good L-asparaginase producers and thus could be used for L-asparaginase production

scholarly journals Parametric Optimization of Cultural Conditions for Carboxymethyl Cellulase Production Using Pretreated Rice Straw by Bacillus sp. 313SI under Stationary and Shaking Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Varsha Goyal ◽  
Arpana Mittal ◽  
Anish Kumari Bhuwal ◽  
Gulab Singh ◽  
Anita Yadav ◽  
...  
Keyword(s):  
Rice Straw ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Cellulosic Biomass ◽  
Ecological Niches ◽  
Bacillus Sp ◽  
Carboxymethyl Cellulase ◽  
Cultural Conditions ◽  
Optimized Conditions

Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass. Out of total 80 microbial isolates from different ecological niches one bacterial strain, identified as Bacillus sp. 313SI, was selected for CMCase production under stationary as well as shaking conditions of growth. During two-stage pretreatment, rice straw was first treated with 0.5 M KOH to remove lignin followed by treatment with 0.1 N H2SO4 for removal of hemicellulose. The maximum carboxymethyl cellulase activity of 3.08 U/mL was obtained using 1% (w/v) pretreated rice straw with 1% (v/v) inoculum, pH 8.0 at 35°C after 60 h of growth under stationary conditions, while the same was obtained as 4.15 U/mL using 0.75% (w/v) pretreated substrate with 0.4% (v/v) inoculum, pH 8.0 at 30°C, under shaking conditions of growth for 48 h. For maximum titre of CMCase carboxymethyl cellulose was optimized as the best carbon source under both cultural conditions while ammonium sulphate and ammonium nitrate were optimized as the best nitrogen sources under stationary and shaking conditions, respectively. The present study provides the useful data about the optimized conditions for CMCase production by Bacillus sp. 313SI from pretreated rice straw.

scholarly journals Biocatalyst: Cellulase Production in Solid State Fermentation (SSF) Using Rice Bran as Substrate

2020 ◽  
Vol 11 (1) ◽  
pp. 7689-7699
Keyword(s):  
Rice Bran ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Glucose Carbon ◽  
Biological Transformation ◽  
Fermentation Period ◽  
Filter Paper Assay

The study was aimed to analyze the biological transformation of cellulose in rice bran by Aspergillus flavus SB04 in SSF for 28 days. The culture conditions such as pH, temperature, moisture content were optimized for the effective production of the enzyme in SSF. Effect of carbon and nitrogen sources on cellulase production was further estimated in SMF and were quantified for 24hrs intervals for 7 days Maximum cellulase production for rice bran was observed to be high in glucose (carbon source) and yeast extract (nitrogen source) at initial moisture 75ml, pH 6, temperature 33°C and fermentation period was 14th day that was optimized using response surface methodology. The enzyme production was analyzed individually by dinitrosalicylic acid (DNS) method, Lowry protein estimation, and filter paper assay. The lignocellulosic degradation was observed and confirmed by FTIR and SEM. The degradation of cellulose periodically increases after 7 days, which influences the yield of cellulase enzyme.

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