scholarly journals Involvement of Physical Parameters in Medium Improvement for Tannase Production by Aspergillus niger FETL FT3 in Submerged Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
I. Darah ◽  
G. Sumathi ◽  
K. Jain ◽  
Lim Sheh Hong
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Incubation Temperature ◽  
Agitation Speed ◽  
Physical Parameters ◽  
Producer Strain ◽  
Medium Ph ◽  
Rhizophora Apiculata ◽  
Maximal Yield ◽  
Initial Medium

Aspergillus niger FETL FT3, a local extracellular tannase producer strain that was isolated from one of dumping sites of tannin-rich barks of Rhizophora apiculata in Perak, Malaysia. This fungus was cultivated in 250 mL Erlenmeyer flask under submerged fermentation system. Various physical parameters were studied in order to maximize the tannase production. Maximal yield of tannase production, that is, 2.81 U per mL was obtained on the fourth day of cultivation when the submerged fermentation was carried out using liquid Czapek-Dox medium containing (percent; weight per volume) 0.25% NaNO3, 0.1% KH2PO4, 0.05% MgSO4 ·7H2O, 0.05% KCl, and 1.0% tannic acid. The physical parameters used initial medium pH of 6.0, incubation temperature of , agitation speed of 200 rpm and inoculums size of  spores/ ml. This research has showed that physical parameters were influenced the tannase production by the fungus with 156.4 percent increment.

scholarly journals Effect of Different Operational Parameters on Bio-degradation of Chicken Feathers by Aspergillus niger: Investigation Under Submerged Fermentation Process

2020 ◽  
Vol 63 (2) ◽  
pp. 71-76
Author(s):  
Faisal Javeed ◽  
Memuna Ghafoor Shahid ◽  
Ali Javed
Keyword(s):  
Aspergillus Niger ◽  
Yeast Extract ◽  
Substrate Concentration ◽  
Submerged Fermentation ◽  
Fermentation Process ◽  
Incubation Temperature ◽  
Fermentation Medium ◽  
Potential Candidate ◽  
Operational Parameters ◽  
Chicken Feathers

Fungal strain, Aspergillus niger (ATCC 1015) has ability to grow on keratinous material therefore, it was selected for the investigating bio-degradation of chicken feathers. Different operational parameters were studied under submerged fermentation process i.e. effect of substrate concentration, effect of pH, effect of incubation temperature, effect of yeast extract concentration and effect of volume of fermentation medium. A. niger was grown on solid medium of malt extract and agar, due to its ability of rapid growth on it. Complete bio-degradation of the substrate was achieved after 5 days (0.70±0.03 U/mL) under standard optimized conditions. Investigation of different operational parameters on bio-degradation of chicken feathers revealed, maximum keratinolytic was observed at 40 °C incubation temperature, at 0.5 g/100 mL of substrate concentration, 8 g/100 mL concentration of yeast extract, at 7 pH of the fermentation medium and at 50 mL volume of fermentation medium. The present study suggests that A. niger could prove to be a potential candidate for production of keratinase and bio-degradation of chicken feathers.  

scholarly journals Pectinase production by Aspergillus niger isolated from decomposed apple skin

2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Point Of View ◽  
Media Composition ◽  
Maximal Level ◽  
Pectinolytic Activity ◽  
Pectinase Activity ◽  
Pectinase Production

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013

scholarly journals The effect of low initial medium pH on in vitro white poplar growth

2013 ◽  
pp. 67-80
Author(s):  
Branislav Kovacevic ◽  
Dragana Miladinovic ◽  
Marina Katanic ◽  
Zoran Tomovic ◽  
Sasa Pekec
Keyword(s):  
Biomass Accumulation ◽  
Dry Mass ◽  
White Poplar ◽  
Medium Ph ◽  
Shoot Height ◽  
Initial Ph ◽  
The Media ◽  
Initial Medium ◽  
Shoot Mass

The effect of low initial medium pH on shoot and root development of five white poplar (Populus alba L.) genotypes was tested. The shoot height, fresh mass of shoots per jar, dry mass of shoots per jar, number of roots, as well as the length of the longest root were measured and final pH of the media determined, after 35 days of culture in vitro. Three initial pH values of the medium were tested: 3.0, 4.0 and 5.5 as control. Agar solidification at pH 3.0 was not achieved after sterilization in autoclave, but it was successful after sterilizing in a microwave oven. The obtained results indicate that the tested genotypes are able to significantly influence the changes of media pH during culture. The effect of differences among the examined media was significant for biomass accumulation and final media pH. Generally, significantly higher values of fresh and dry shoot mass, shoot height and the longest root length were recorded on a medium with initial pH 3.0 then on a standard medium with pH 5.5.The implications of the obtained results for the improvement of in vitro propagation of white poplars are discussed.

Studies on the Biosynthesis of Enzyme Xylanase by Submerged Fermentation from Aspergillus niger GCBMX-45

2002 ◽  
Vol 5 (12) ◽  
pp. 1309-1310 ◽  
Author(s):  
Ikram-ul- Haq ◽  
Amna Ehsan ◽  
Waseem Ahmad Butt ◽  
Sikander Ali
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation

Control of morphogenetic pathways in thin cell layers of tobacco by pH

1989 ◽  
Vol 67 (3) ◽  
pp. 650-654 ◽  
Author(s):  
A. Cousson ◽  
P. Toubart ◽  
K. Tran Thanh Van
Keyword(s):  
Butyric Acid ◽  
De Novo ◽  
Culture Media ◽  
Callus Formation ◽  
Medium Ph ◽  
Vegetative Buds ◽  
Cell Layers ◽  
Initial Medium ◽  
Vegetative Bud

Thin cell layer explants of tobacco were floated in vitro on the surface of liquid culture media. The initial exogenous concentrations of indolyl-3-butyric acid, and kinetin, the initial medium pH, and the explant density were varied. Various patterns of de novo and direct differentiation without any intermediate callus (flower, vegetative bud, root) as well as the absence of morphogenesis and callus formation without any subsequent organogenesis were separately controlled on 100% of the explants. On the same exogenous combination of glucose, indolyl-3-butyric acid, and kinetin, changes in initial medium pH changed the pattern of morphogenesis. For a given initial exogenous indolyl-3-butyric acid concentration, vegetative buds were obtained at either pH 6.1 or 7.8, whereas a mixture of flowers and vegetative buds was obtained at pH 6.8. Furthermore, changes in explant density changed the morphogenetic response. It is suggested that the effects of the initial medium pH and explant density on morphogenesis may be related partially to modifications of the physicochemical properties of the cell wall and (or) plasmalemma.

Production of acidic lipase by a mutant of Aspergillus niger NCIM 1207 in submerged fermentation

2004 ◽  
Vol 39 (12) ◽  
pp. 2031-2034 ◽  
Author(s):  
Nutan D. Mahadik ◽  
Kulbhushan B. Bastawde ◽  
Ulka S. Puntambekar ◽  
Jayant M. Khire ◽  
Digambar V. Gokhale
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Acidic Lipase
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