scholarly journals Effect of Different Operational Parameters on Bio-degradation of Chicken Feathers by Aspergillus niger: Investigation Under Submerged Fermentation Process

2020 ◽  
Vol 63 (2) ◽  
pp. 71-76
Author(s):  
Faisal Javeed ◽  
Memuna Ghafoor Shahid ◽  
Ali Javed
Keyword(s):  
Aspergillus Niger ◽  
Yeast Extract ◽  
Substrate Concentration ◽  
Submerged Fermentation ◽  
Fermentation Process ◽  
Incubation Temperature ◽  
Fermentation Medium ◽  
Potential Candidate ◽  
Operational Parameters ◽  
Chicken Feathers

Fungal strain, Aspergillus niger (ATCC 1015) has ability to grow on keratinous material therefore, it was selected for the investigating bio-degradation of chicken feathers. Different operational parameters were studied under submerged fermentation process i.e. effect of substrate concentration, effect of pH, effect of incubation temperature, effect of yeast extract concentration and effect of volume of fermentation medium. A. niger was grown on solid medium of malt extract and agar, due to its ability of rapid growth on it. Complete bio-degradation of the substrate was achieved after 5 days (0.70±0.03 U/mL) under standard optimized conditions. Investigation of different operational parameters on bio-degradation of chicken feathers revealed, maximum keratinolytic was observed at 40 °C incubation temperature, at 0.5 g/100 mL of substrate concentration, 8 g/100 mL concentration of yeast extract, at 7 pH of the fermentation medium and at 50 mL volume of fermentation medium. The present study suggests that A. niger could prove to be a potential candidate for production of keratinase and bio-degradation of chicken feathers.  

scholarly journals Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Sri Sugiwati ◽  
Muhammad Hanafi ◽  
Hanifah Lioe ◽  
Maggy Suhartono
Keyword(s):  
Aspergillus Niger ◽  
Rice Bran ◽  
Incubation Time ◽  
Incubation Temperature ◽  
Glycosyl Hydrolase ◽  
Fermentation Medium ◽  
Growth Conditions ◽  
Mineral Salts ◽  
Local Isolate ◽  
Substrate Ratio

Abstract. Sugiwati S, Hanafi M, Lioe HN, Suhartono MT. 2020.  Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate. Biodiversitas 21: 4058-4066. β-Glucosidase is the family of glycosyl hydrolase that have potential role in various food industry, such as in tea, wine and vanilla industries to increase the aroma and production of isoflavone aglycons in soybean flour. The present work produced β-glucosidase from local isolate of Aspergillus niger InaCC F57 under solid-state fermentation (SSF) using rice bran substrate. Fermentation process was made in various conditions with respect to carbon source as substrate, initial pH of fermentation medium, incubation time, water to substrate ratio, fermentation temperature, and addition of Mandels mineral salts solution. The results showed that activity of β-glucosidase was best at, i.e., 2.45 U/mL, with the use of rice bran as substrate. Furthermore, optimum condition for the highest production of β-glucosidase occurred at pH 2.0, incubation time of 5 days, water to substrate ratio of 1.5: 1, and incubation temperature of 32°C. Additionally, in optimum fermentation conditions, production of β-glucosidase could be enhanced up to 26.22% with the presence of Mandels mineral salts solution as compared to the control.

Production of L-Asparaginase from Aspergillus Niger using Agro Wastes By-Products in Submerged Fermentation Process

2013 ◽  
Vol 62 (2) ◽  
Author(s):  
Muhammad Anjum Zia Anjum Zia ◽  
Rabia Bashir ◽  
Ishtiaq Ahmed ◽  
Tehreema Iftikhar
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Fermentation Process ◽  
Corn Steep Liquor ◽  
Maximum Yield ◽  
Growth Media ◽  
Organic Salts ◽  
Waste Products ◽  
Fermentation Time ◽  
By Products

The project was carried out to obtain maximum yield of L-asparaginase from Aspergillus niger using by-products of agro wastes incorporated with organic salts in submerged fermentation process. The main objective of the project was to study the kinetic parameters of L-asparaginase productivity. After optimization maximum enzyme activity (2.83U/mL±0.065) was achieved using corn steep liquor as a substrate and with 4% inoculum, pH 6.5, 1% substrate concentration, 96 hrs fermentation time period and 1% glucose was used as additional supplement to the growth media to obtain better yield of L-asparaginase. This study showed that glucose concentration beyond 1% suppressed the enzymatic activity. From the results it can be concluded that L-Asparaginase production was optimized when cheap agro-waste products were used as a substrate at low concentrations and under acidic conditions. Its relative stability in acidic pH conditions make it ideal for applications in health care systems and pharmaceutical industry.

scholarly journals Enhanced Production, Purification and Characterization of Alkaline Keratinase from Streptomyces minutiscleroticus DNA38

2015 ◽  
Vol 43 ◽  
pp. 27-37 ◽  
Author(s):  
Allure Nandini ◽  
D.N. Madhusudhan ◽  
Agsar Dayanand
Keyword(s):  
Yeast Extract ◽  
Incubation Temperature ◽  
Direct Action ◽  
Interactive Effect ◽  
Chicken Feathers ◽  
Enhanced Production ◽  
Optimized Conditions ◽  
Critical Process ◽  
Central Composite

A thermo tolerant, feather-degrading, newly isolated actinobacterial strain Streptomyces minutiscleroticus DNA38 was investigated for its ability to produce keratinase. Maximum production (283.4 IU) of keratinase by Streptomyces minutiscleroticus DNA38 in starch chicken feathers medium under submerged bioprocess was observed at optimized conditions of pH 9.0 of the medium and 45 °C incubation temperature. Further, an enhanced production (435.8 IU) of keratinase was achieved employing response surface methodology. Combined interactive effect of starch (7.50 g/L), yeast extract (0.74 g/L) and chicken feathers (7.50 g/L) were found to be the critical process variables for enhanced production under central composite design. Chicken feathers showed a direct action and addition of starch and yeast extract to the medium proved effective for a significant increase in the production of keratinase. The purified keratinase was monomeric and had a molecular mass of 29 kDa. The enzyme activity was significantly inhibited after pH 9.0 and temperature 50 °C.

scholarly journals Involvement of Physical Parameters in Medium Improvement for Tannase Production by Aspergillus niger FETL FT3 in Submerged Fermentation

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
I. Darah ◽  
G. Sumathi ◽  
K. Jain ◽  
Lim Sheh Hong
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Incubation Temperature ◽  
Agitation Speed ◽  
Physical Parameters ◽  
Producer Strain ◽  
Medium Ph ◽  
Rhizophora Apiculata ◽  
Maximal Yield ◽  
Initial Medium

Aspergillus niger FETL FT3, a local extracellular tannase producer strain that was isolated from one of dumping sites of tannin-rich barks of Rhizophora apiculata in Perak, Malaysia. This fungus was cultivated in 250 mL Erlenmeyer flask under submerged fermentation system. Various physical parameters were studied in order to maximize the tannase production. Maximal yield of tannase production, that is, 2.81 U per mL was obtained on the fourth day of cultivation when the submerged fermentation was carried out using liquid Czapek-Dox medium containing (percent; weight per volume) 0.25% NaNO3, 0.1% KH2PO4, 0.05% MgSO4 ·7H2O, 0.05% KCl, and 1.0% tannic acid. The physical parameters used initial medium pH of 6.0, incubation temperature of , agitation speed of 200 rpm and inoculums size of  spores/ ml. This research has showed that physical parameters were influenced the tannase production by the fungus with 156.4 percent increment.

scholarly journals Statistical optimization of an acid protease production by a local Aspergillus niger MH109542 using a medium based on decommissioned dates

2020 ◽  
pp. 68-76
Author(s):  
Malika Benkahoul ◽  
Amina Bramki ◽  
Aicha Belmessikh ◽  
Aicha Mechakra-Maza
Keyword(s):  
Aspergillus Niger ◽  
Yeast Extract ◽  
Corn Steep Liquor ◽  
Basal Medium ◽  
Extreme Environment ◽  
Fermentation Medium ◽  
Acid Protease ◽  
Protease Production ◽  
Plackett And Burman ◽  
Steep Liquor

The production of an acid protease by liquid fermentation is performed on a medium based on decommissioned dates by a local mold isolated from an extreme environment. The used mold is isolated from the thermal soil in Hammam Safsaf of Teleghma (Algeria). Phenotypic and molecular identification has shown that it is Aspergillus niger. This fungi strain exhibited exo-protease activity on milk agar. The fermentation medium based on decommissioned dates is enriched by other factors according to a statistical method; the plan of Plackett and Burman. This method (N = 8 experiments and N-1 factors) allowed the optimization of the enzyme production and the growth of the mold. The statistical analysis of the obtained results shows an increased enzyme activity (650.20U), in the presence of yeast extract and salts with probabilities p = 0.239 for the first and p = 0.190 for the second. Furthermore, the corn-steep-liquor (p = 0.229) and yeast extract (p = 0.053) present a significant effect on mold growth. In conclusion, the culture of A. niger on optimized medium gives good yields of biomass and proteolytic activity compared to the basal medium. These results are encouraging. In fact, the use of a cheap and available substrate such as decommissioned dates saves the cost price of fermentation on an industrial scale.

Effect of span 20 concentration on oxalic acid production from post-refining fatty acids by Aspergillus niger XP

2006 ◽  
Vol 60 (5) ◽  
Author(s):  
I. Musial ◽  
W. Rymowicz ◽  
D. Witkowska
Keyword(s):  
Fatty Acids ◽  
Aspergillus Niger ◽  
Oxalic Acid ◽  
Submerged Fermentation ◽  
Acid Production ◽  
Fermentation Medium

AbstractSubmerged fermentation experiments were carried out to study the stimulating effects of the surfactant Span 20 on the growth of Aspergillus niger XP mutant and oxalic acid production from the post-refining fatty acids. Span 20 concentration of 0.75 g dm−3 was found to be the most suitable for oxalic acid production from fatty acids. Using this dose and a fermentation medium containing 30 g dm−3 of post-refining fatty acids, the oxalic acid production, oxalate yield, and overall oxalate productivity were the highest.

scholarly journals Application of Komagataeibacter saccharivorans A2 strain to produce “Táo mèo” vinegar by submerged fermentation method

2017 ◽  
Vol 33 (1S) ◽  
Author(s):  
Pham Thi Hau ◽  
Phung Thi Thanh Tu ◽  
Bui Thi Thuy Ha ◽  
Nguyen Thi Viet Anh
Keyword(s):  
Acetic Acid ◽  
Yeast Extract ◽  
Submerged Fermentation ◽  
Fermentation Process ◽  
Aerobic Condition ◽  
Technological Parameters ◽  
Process Technology ◽  
Apple Cider ◽  
Food Industries ◽  
Fermentation Method

“Táo mèo” vinegar is a product of Vietnam, which is made during which is made during the fermentation of apple cider in aerobic condition by acetic bacteria. In this study, we use a submerged fermentation method to produced “Táo mèo” vinegar from apple cider (Docynia indica) or Son Tra fruit. Based on the liquor obtained from the process of producing cider liquor of the Food Industries Research Institute, we conducted a survey of the technological parameters affect the fermentation process. We have established a process technology to produce apple cider vinegar with the following parameters: Strain K. saccharivorans A2: 9%; initial alcohol: 6%; initial acetic acid: 0,6%; saccharose: 9g/l; yeast extract 0,5g/l; glycerol: 0,5g/l; Mineral: MgSO4.7H2O: 0,25g/l, KH2PO4: 0,25g/l, (NH4)2HPO4: 0,5g /l.  

scholarly journals Pectinase production by Aspergillus niger isolated from decomposed apple skin

2013 ◽  
Vol 48 (1) ◽  
pp. 25-32 ◽  
Author(s):  
S Islam ◽  
B Feroza ◽  
AKMR Alam ◽  
S Begum
Keyword(s):  
Aspergillus Niger ◽  
Incubation Temperature ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Point Of View ◽  
Media Composition ◽  
Maximal Level ◽  
Pectinolytic Activity ◽  
Pectinase Activity ◽  
Pectinase Production

Pectinase activity among twelve different fungal strains, Aspergillus niger IM09 was identified as a potential one to produce maximal level 831 U/g at pH 4.0. Media composition, incubation temperature, incubation time, substrate concentration, aeration, inoculum size, assay temperature and nitrogen sources were found to effect pectinase activity. Moisture content did not affect the activity significantly. Media composition was varied to optimize the enzyme production in solid state fermentation. It was observed that the highest pectinase activity of 831.0 U/g was found to produce in presence of yeast extract as a nitrogen source in combination with ammonium sulfate in assay media. Aeration showed positive significant effects on pectinase production 755 U/g at 1000 ml flasks. The highest pectinase production was found at 2 g pectin (521 U/g) used as a substrate. Pectinolytic activity was found to have undergone catabolite repression with higher pectin concentration (205 U/g at 5 g pectin). The incubation period to achieve maximum pectinase activity by the isolated strain Aspergillus niger IM09 was 3 days, which is suitable from the commercial point of view. DOI: http://dx.doi.org/10.3329/bjsir.v48i1.15410 Bangladesh J. Sci. Ind. Res. 48(1), 25-32, 2013

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