scholarly journals IMPLEMENTATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF INORGANIC ARSENIC SPECIES IN OCCUPATIONALLY EXPOSED HUMAN URINE SAMPLES AND ITS TOXIC EFFECTS ON EPITHELIAL CELLS OF RENAL COLLECTING TUBULE

2016 ◽  
Vol 61 (4) ◽  
pp. 3214-3218 ◽  
Author(s):  
NICOLE ROLDÁN ◽  
NICOLÁS SALINAS-PARRA ◽  
ALEXIS A GONZALEZ ◽  
FLAVIA CIFUENTES-ARANEDA ◽  
HELEN ARIAS ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Urine ◽  
Analytical Method ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Urine Samples ◽  
Collecting Tubule ◽  
Occupationally Exposed ◽  
Inorganic Arsenic Species

Development of analytical method for determination of Sb(V), Sb(III) and TMSb(V) in occupationally exposed human urine samples by HPLC–HG-AFS

2011 ◽  
Vol 97 (1) ◽  
pp. 78-84 ◽  
Author(s):  
Waldo Quiroz ◽  
Helen Arias ◽  
Manuel Bravo ◽  
Marcela Pinto ◽  
María Gabriela Lobos ◽  
...  
Keyword(s):  
Human Urine ◽  
Analytical Method ◽  
Urine Samples ◽  
Human Urine Samples ◽  
Occupationally Exposed

scholarly journals Effect of arsenosugar ingestion on urinary arsenic speciation

1998 ◽  
Vol 44 (3) ◽  
pp. 539-550 ◽  
Author(s):  
Mingsheng Ma ◽  
X Chris Le
Keyword(s):  
Arsenic Speciation ◽  
Inorganic Arsenic ◽  
Sample Pretreatment ◽  
Arsenic Species ◽  
Urine Samples ◽  
Standard Reference Materials ◽  
Dimethylarsinic Acid ◽  
Urinary Arsenic ◽  
Biomarkers Of Exposure

Abstract We developed and evaluated a method for the determination of μg/L concentrations of individual arsenic species in urine samples. We have mainly studied arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMAA), and dimethylarsinic acid (DMAA) because these are the most commonly used biomarkers of exposure by the general population to inorganic arsenic and because of concerns over these arsenic species on their toxicity and carcinogenicity. We have also detected five unidentified urinary arsenic species resulting from the metabolism of arsenosugars. We combined ion pair liquid chromatography with on-line hydride generation and subsequent atomic fluorescence detection (HPLC/HGAFS). Detection limits, determined as three times the standard deviation of the baseline noise, are 0.8, 1.2, 0.7, and 1.0 μ/L arsenic for arsenite, arsenate, MMAA, and DMAA, respectively. These correspond to 16, 24, 14, and 20 pg of arsenic, respectively, for a 20-μL sample injected for analysis. The excellent detection limit enabled us to determine trace concentrations of arsenic species in urine samples from healthy subjects who did not have excess exposure to arsenic. There was no need for any sample pretreatment step. We used Standard Reference Materials, containing both normal and increased concentrations of arsenic, to validate the method. Interlaboratory studies with independent techniques also confirmed the results obtained with the HPLC/HGAFS method. We demonstrated an application of the method to the determination of arsenic species in urine samples after the ingestion of seaweed by four volunteers. We observed substantial increases of DMAA concentrations in the samples collected from the volunteers after the consumption of seaweed. The increase of urinary DMAA concentration is due to the metabolism of arsenosugars that are present in the seaweed. Our results suggest that the commonly used biomarkers of exposure to inorganic arsenic, based on the measurement of arsenite, arsenate, MMAA, and DMAA, are not reliable when arsenosugars are ingested from the diet.

Atomic fluorescence determination of total and inorganic arsenic species in beer

2001 ◽  
Vol 16 (7) ◽  
pp. 762-766 ◽  
Author(s):  
Amparo Martinez ◽  
Angel Morales-Rubio ◽  
M. Luisa Cervera ◽  
Miguel de la Guardia
Keyword(s):  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Atomic Fluorescence ◽  
Fluorescence Determination ◽  
Inorganic Arsenic Species

Determination of inorganic arsenic species in natural waters—Benefits of separation and preconcentration on ion exchange and hybrid resins

2010 ◽  
Vol 673 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Nureddin Ben Issa ◽  
Vladana N. Rajaković-Ognjanović ◽  
Branislava M. Jovanović ◽  
Ljubinka V. Rajaković
Keyword(s):  
Ion Exchange ◽  
Natural Waters ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Separation And Preconcentration ◽  
Inorganic Arsenic Species

scholarly journals Stability of inorganic arsenic species in simulated raw waters with the presence of NOM

2006 ◽  
Vol 6 (6) ◽  
pp. 175-182 ◽  
Author(s):  
G.J. Liu ◽  
X.R. Zhang ◽  
J. Jain ◽  
J.W. Talley ◽  
C.R. Neal
Keyword(s):  
Natural Organic Matter ◽  
Inductively Coupled Plasma ◽  
Inorganic Arsenic ◽  
Arsenic Species ◽  
Inductively Coupled ◽  
Plasma Mass Spectrometry ◽  
Mass Spectrometry System ◽  
The Stability ◽  
Inorganic Arsenic Species

Effect of natural organic matter (NOM) on the stability of inorganic arsenic species in simulated raw water was examined at circumneutral pH. An ion chromatography–inductively coupled plasma mass spectrometry system was used for simultaneous determination of As(III) and As(V). A reduction of arsenate (As(V)) to arsenite (As(III)) was observed in the unfiltered simulated raw waters (USW). The As(V) reduction to As(III) did not occur in the simulated waters that passed through a 0.2 μm membrane (FSW). Microorganism activities is probably the major reason causing As(V) reduction in the USW. In the FSW without NOM, As(III) tended to be oxidized into As(V). The addition of 0.036 mM of Fe(II) significantly facilitated the oxidation. The presence of 10 mg/L Suwannee River NOM as C inhibited As(III) oxidation no matter whether Fe(II) existed or not. The experimental results suggest that NOM can mediate distribution of inorganic arsenic species in water, thus it is an important factor controlling the mobility and toxicity of arsenic in drinking water.

Cathodic Stripping Voltammetric Study for Determination of Inorganic Arsenic Species in Seaweed Gracilaria fisheri

2019 ◽  
Vol 886 ◽  
pp. 78-85
Author(s):  
Charuwan Khamkaew ◽  
Lalitporn Wongsuwan ◽  
Abdullateep Sareedeh
Keyword(s):  
Inorganic Arsenic ◽  
Stripping Voltammetry ◽  
Arsenic Species ◽  
Deposition Efficiency ◽  
Relative Standard ◽  
Inorganic As ◽  
Inorganic Arsenic Species ◽  
Deposition Step ◽  
Cathodic Stripping

A simple, rapid, selective and sensitive square wave cathodic stripping voltammetry (SWCSV) at a hanging mercury drop electrode (HMDE) for the determination of As(III), As(V) and inorganic As(total) in seaweed was developed. The method was based on the formation of copper-arsenic intermetallic compound in the presence of HCl onto a HMDE at a constant potential of -0.40 V vs. Ag/AgCl in the deposition step, followed by the reduction of deposited compound to arsine at a potential of -0.78 V in the stripping step. In the system, only As(III) was directly measured, whereas inorganic As(total) was measured by reducing As(V) to As(III) using thiosulfate. In the deposition step, the use of 10 mg L-1 Cu(II) and 1 M HCl was recommended for the determination of 2 µg L-1 As(III) under the optimum instrumental variables obtained at 150 s for deposition time and 2000 rpm for stirring speed with the highest deposition efficiency of 16.15 and 88.49%, respectively. In the determination of As(V), two values of the highest reduction efficiency obtained using concentration of 40 mg L-1 thiosulfate, and reducing time at 300 s were 98.15% and 37.89%, respectively. In the measurement, the quantification limits of As(III) and As(V) were 0.46, and 1.62 µg L-1, respectively. The relative standard deviation (n=10) for 2 ug L-1 As(III) and As(V) were 5.20% and 2.57%, respectively. The proposed method was applied to the determination of inorganic arsenic species in seaweed Gracilaria fisheri.

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