scholarly journals Assessing Clivia taxonomy using the core DNA barcode regions, matK and rbcLa

Bothalia ◽  
2018 ◽  
Vol 48 (1) ◽  
Author(s):  
Johan J. Spies ◽  
Paula Spies
Keyword(s):  
Dna Barcode ◽  
Taxonomic Revision ◽  
Dna Barcodes ◽  
Eastern Cape ◽  
Base Pairs ◽  
The Core ◽  
The North ◽  
Kwazulu Natal ◽  
North Eastern ◽  
Species Specific

Background: Clivia is a genus of the family Amaryllidaceae endemic to South Africa and Swaziland. Six species and one natural hybrid have been described. Some morphological traits overlap between some species, thus causing taxonomic confusion.Objectives: The discriminatory power of the core DNA barcodes (matK and rbcLa) was evaluated, and the current taxonomy of Clivia was assessed.Method: Seventy-four two-locus DNA barcodes from 4 to 18 specimens per species were generated.Results: The matK region had a higher mean intraspecific variation of 0.21 compared with the 0.02 of rbcLa. The two-locus barcodes have an aligned length of 1335 base pairs. Three species, Clivia mirabilis, Clivia nobilis and Clivia caulescens, are monophyletic in the Bayesian Inference (BI) cladogram. The remaining Clivia species (Clivia miniata, Clivia gardenii, Clivia robusta and their affinities) are paraphyletic. Clivia is divided into 17 haplogroups with those of C. mirabilis and C. nobilis being unique. Clivia caulescens has three haplotypes. The Clivia species from the north-eastern distribution range of the Eastern Cape and KwaZulu-Natal provinces have 11 haplogroups and no species-specific DNA barcodes. These groups have no correlation with the current taxonomy or geographical distribution.Conclusions: Only 37.33% of the species can be correctly identified with the ‘best match’ option in SpeciesIdentifier. Clivia mirabilis, C. nobilis and C. caulescens have unique DNA barcodes to identify them. Specimens from the Ngome area in KwaZulu-Natal have a unique DNA barcode, separating them from the rest of C. gardenii. A taxonomic revision is suggested.

Taxonomic revision of South African Memecylon (Melastomataceae—Olisbeoideae), including three new species

Phytotaxa ◽  
2019 ◽  
Vol 418 (3) ◽  
pp. 237-257
Author(s):  
ROBERT DOUGLAS STONE ◽  
IMERCIA GRACIOUS MONA ◽  
DAVID STYLES ◽  
JOHN BURROWS ◽  
SYD RAMDHANI
Keyword(s):  
South African ◽  
Taxonomic Revision ◽  
Limpopo Province ◽  
Eastern Cape ◽  
African Species ◽  
Kwazulu Natal ◽  
North Eastern ◽  
The Status ◽  
Three New Species ◽  
Vegetative Characters

Earlier works recognised two South African species Memecylon bachmannii and M. natalense within M. sect. Buxifolia, but recent molecular analyses have revealed that M. natalense as previously circumscribed is not monophyletic and includes several geographically outlying populations warranting treatment as distinct taxa. In this revision we recognise five endemic South African species of which M. bachmannii and M. natalense are both maintained but with narrower circumscriptions, and M. kosiense, M. soutpansbergense and M. australissimum are newly described. Memecylon kosiense is localised in north-eastern KwaZulu-Natal (Maputaland) and is closely related to M. incisilobum of southern Mozambique. Memecylon soutpansbergense, from Limpopo Province, was previously confused with M. natalense but is clearly distinguished on vegetative characters. Memecylon australissimum occurs in the Eastern Cape (Hluleka and Dwesa-Cwebe nature reserves) and has relatively small leaves like those of M. natalense, but the floral bracteoles are persistent and the fruit is ovoid as in M. bachmannii. We further designate a lectotype for M. natalense, provide an updated key to the South African species of Memecylon, and provisionally assess the status of each species in accordance with International Union for Conservation of Nature criteria.

Taxonomy of Nystiellidae (Caenogastropoda, Epitonioidea) from Brazil, with description of three new species from the South-western Atlantic

2017 ◽  
Vol 98 (7) ◽  
pp. 1619-1644 ◽  
Author(s):  
Alexandre Dias Pimenta ◽  
Bruno Garcia Andrade ◽  
Ricardo Silva Absalão
Keyword(s):  
Rio Grande ◽  
Taxonomic Revision ◽  
South Atlantic ◽  
Shell Morphology ◽  
The South ◽  
Western Atlantic ◽  
North East ◽  
The North ◽  
Eastern Brazil ◽  
North Eastern

A taxonomic revision of the Nystiellidae from Brazil, including samples from the Rio Grande Rise, South Atlantic, was performed based on shell morphology. Five genera and 17 species were recognized. For the richest genus,Eccliseogyra, the three species previously recorded from Brazil were revised:E. brasiliensisandE. maracatu, previously known only from their respective type series, were re-examined. Newly available material ofE. maracatuexpanded the known geographic range of this species to off south-east Brazil.Eccliseogyra nitidais now recorded from north-eastern to south-eastern Brazil, as well as from the Rio Grande Rise. Three species ofEccliseogyraare newly recorded from the South Atlantic:E. monnioti, previously known from the north-eastern Atlantic, occurs off eastern Brazil and on the Rio Grande Rise; its protoconch is described for the first time, confirming its family allocation.Eccliseogyra pyrrhiasoccurs off eastern Brazil and on the Rio Grande Rise, andE. folinioff eastern Brazil. The genusIphitusis newly recorded from the South Atlantic.Iphitus robertsiwas found off northern Brazil, although the shells show some differences from the type material, with less-pronounced spiral keels. Additional new finds showed thatIphitus cancellatusranges from eastern Brazil to the Rio Grande Rise, and Iphitusnotiossp. nov. is restricted to the Rio Grande Rise.Narrimania, previously recorded from Brazil based on dubious records, is confirmed, including the only two living species described for the genus:N. azelotes, previously only known from the type locality in Florida, andN. concinna, previously known from the Mediterranean. A third species,Narrimania raquelaesp. nov. is described from eastern Brazil, diagnosed by its numerous and thinner cancellate sculpture. To the three species ofOpaliopsispreviously known from Brazil, a fourth species,O. arnaldoisp. nov., is added from eastern Brazil, and diagnosed by its very thin spiral sculpture, absence of a varix, and thinner microscopic parallel axial striae.Papuliscala nordestina, originally described from north-east Brazil, is recorded off eastern Brazil and synonymized withP. elongata, a species previously known only from the North Atlantic.

Taxonomic revision of the Neotropical diving beetles genus Laccodytes Régimbart, 1895 (Coleoptera: Dytiscidae)

Zootaxa ◽  
2010 ◽  
Vol 2347 (1) ◽  
pp. 37 ◽  
Author(s):  
MARIO TOLEDO ◽  
PAUL J. SPANGLER ◽  
MICHAEL BALKE
Keyword(s):  
South America ◽  
Species Identification ◽  
Taxonomic Revision ◽  
Diagnostic Features ◽  
The North ◽  
Centre Of Diversity ◽  
Diving Beetles ◽  
North Eastern ◽  
Species Groups ◽  
North Eastern Part

The Neotropical Laccophilinae genus Laccodytes Régimbart, 1895 is redefined and revised. We recognize ten species, six of which are described as new. We define two species groups: the Laccodytes apalodes-group (L. apalodes Guignot, 1955, L. rondonia sp.n.), and the L. phalacroides-group (L. americanus Peschet, 1919, L. obscuratus sp.n., L. bassignanii sp.n., L. neblinae sp.n., L. olibroides Régimbart, 1895, L. phalacroides Régimbart, 1895, L. takutuanus sp.n., L. androginus sp.n.). Laccophilus pumilio LeConte, 1878, assigned to Laccodytes by Young (1954), belongs to an undescribed genus. Laccodytes species are lotic and most of them inhabit the north-eastern part of South America, apparently with Venezuela and Guyana as centre of diversity. Descriptions, illustrations and SEM photos of habitus, genitals and other diagnostic features are provided for each species, together with habitat notes, when known. We provide a key for species identification.

scholarly journals Die lied in die erediens: Die rol wat dit gespeel het in die Afskeiding in 1859 in Suid-Afrika

1999 ◽  
Vol 33 (4) ◽  
Author(s):  
H.A. Louw
Keyword(s):  
South Africa ◽  
Middle Ages ◽  
Old Testament ◽  
Eastern Cape Province ◽  
Eastern Cape ◽  
The North ◽  
North Eastern ◽  
Congregational Singing ◽  
The Church

The role of hymns in public worship and the influence it had on the Schism in 1859 in South Africa During the Middle Ages congregational singing was replaced by choir singing. Both Luther and Calvin agreed that the members of the congregation should actively participate in the worship service by means of song. Calvin limited congregational songs to the Psalms of the Old Testament. The church in Netherlands followed his example, but added some hymns, excluding the Apostles Creed, that comprises also lyrical parts from Scripture. In 1807 a hymn book was implemented and used in the Netherlands. This was one of the reasons for the Schism which took place in 1834. During 1814 the hymn book was implemented in the Cape resulting in discontent in the border districts. Some discontented people took part in the Great Trek. A congregation mainly consisting of these people was established in Rustenburg in 1859. In this congregation only Psalms were sung during services. Soon Reformed congregations having the same objections regarding hymns came into being in the Free State and the north-eastern Cape Province. For the founder of these congregations, Rev. D. Postma, the singing of free hymns was a mediance matter. For the “Doppers” as the conservative people were called, the singing of Psalms only was a serious matter of principle. Times have changed and the Reformed Churches in South Africa will have to reflect whether it is really a matter of principle to sing Old Testament Psalms only. The suffering, death, resurrection and ascension of Christ should also be celebrated in song. The existing 48 scriptural lyrics do not satisfy these requirements. Free hymns of the other Afrikaans churches will definitely have to be taken into consideration.

An overview of the South African tangle-veined flies (Diptera: Nemestrinidae), with an annotated key to the genera and a checklist of species

Zootaxa ◽  
2006 ◽  
Vol 1277 (1) ◽  
pp. 39 ◽  
Author(s):  
DAVID A. BARRACLOUGH
Keyword(s):  
South African ◽  
Taxonomic Revision ◽  
Family Characteristics ◽  
Western Cape ◽  
Eastern Cape ◽  
The South ◽  
African Species ◽  
North West ◽  
Kwazulu Natal ◽  
Closed Canopy

The South African Nemestrinidae are reviewed. Regional family characteristics, biology and the importance of long-proboscid species in pollination biology are discussed. Long-proboscid species (proboscis 15 mm or longer) are important pollinators in most provinces, but particularly in the Western Cape; all have specialised pollination interactions with long-tubed flower species. Five pollination guilds centered on long-proboscid species are briefly discussed.        A detailed, annotated key to the six genera is presented; in it particular attention is given to the problematic distinction between Prosoeca Schiner and Stenobasipteron Lichtwardt. A checklist of all described species considered to have a South African provenance is provided. A total of 43 described species is recorded in the following genera: Moegistorhynchus Macquart (4 species); Prosoeca (35 species); Stenobasipteron (1 species); Atriadops Wandolleck (1 species); Nycterimyia Lichtwardt (1 species); Trichopsidea Westwood (1 species).        Moegistorhynchus is restricted to the west coast (Western Cape, Northern Cape). Three of its species have a remarkably elongate proboscis, the best known and most widespread being M. longirostris (Wiedemann, 1819). Its proboscis reaches 90 to 100 mm in length in some specimens, this being the longest proboscis of all known Diptera. Two new species have been identified in the fauna.        Prosoeca dominates the fauna, and is the only genus likely to be represented in all nine provinces. It appears to occur in all habitats except closed-canopy forest. Prosoeca major Bezzi, 1924 is newly synonymised with P. robusta Bezzi, 1924. The likely South African provenance of P. nigripes (Macquart, 1840) is confirmed. Nemestrina obscura Westwood, 1835, previously referred to Prosoeca, is considered not to be an Afrotropical species. Prosoeca rhodesiensis Bequaert, 1925a, is recorded from South Africa for the first time.        Although Stenobasipteron is restricted to only one named South African species, namely S. wiedemanni Lichtwardt, 1910 from the Eastern Cape and KwaZulu-Natal, several undescribed species occur in Mpumalanga and Limpopo. Stenobasipteron wiedemanni occurs in closed-canopy forest, but species from Mpumalanga may occur in other habitats such as grassland and savanna.        There are at least two species of Atriadops, one almost certainly being A. vespertilio (Loew, 1858). The genus is recorded from the Western Cape, Eastern Cape, KwaZulu-Natal, Gauteng and Mpumalanga. A taxonomic revision is recommended. Nycterimyia is represented by one species only, namely N. capensis Bezzi, 1924 from KwaZulu-Natal and Limpopo. Trichopsidea is represented by one species, T. costata (Loew, 1858), recorded from North West, Gauteng and Limpopo.

scholarly journals A rapid and inexpensive molecular technique to discriminate the north-eastern Atlantic and Mediterranean Atherina species and its potential applications in ecology and larval identification

Biologia ◽  
2012 ◽  
Vol 67 (5) ◽  
Author(s):  
Vítor Carvalho Almada ◽  
Sara Martins Francisco ◽  
Inęs Rosa ◽  
Vera Domingues ◽  
Henrique Cabral ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Molecular Technique ◽  
Formal Description ◽  
The North ◽  
North Eastern ◽  
Potential Applications ◽  
Species Specific ◽  
Specific Nucleotide

AbstractThis note describes a rapid and inexpensive Restriction Fragment Length Polymorphism technique to discriminate all species of Atherina (Pisces: Atherinidae) of the north-eastern Atlantic and the Mediterranean. This technique is based on digestion of a fragment of the 12S ribosomal RNA (12SrRNA) gene region of mitochondrial DNA with restriction enzymes that recognize species-specific nucleotide sites. The three currently recognized species in the area, as well as two additional forms awaiting formal description, can be discriminated using a set of four endonucleases. We argue that this simple and fast technique may be of great help in the identification of young stages and in ecological surveys.

Age-related trends in otolith chemistry of Merluccius merluccius from the north-eastern Atlantic Ocean and the western Mediterranean Sea

2005 ◽  
Vol 56 (5) ◽  
pp. 599 ◽  
Author(s):  
B. Morales-Nin ◽  
S. C. Swan ◽  
J. D. M. Gordon ◽  
M. Palmer ◽  
A. J. Geffen ◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Western Mediterranean ◽  
Elemental Concentrations ◽  
The Core ◽  
The North ◽  
Western Mediterranean Sea ◽  
Growth Increments ◽  
Age Related ◽  
North Eastern ◽  
The Mediterranean

Sagittal otoliths of European hake obtained from five geographic locations in the north-eastern Atlantic and western Mediterranean were examined using laser ablation and inductively coupled plasma mass spectrometry. Otolith sections were analysed for the isotopes 24Mg, 55Mn, 66Zn, 85Rb, 86Sr, 138Ba and 208Pb, measured relative to 43Ca counts. These analyses considered only age 0 (core area) and ages 1 to 3. Age-related trends in otolith elemental composition were observed in hake from all areas, but were masked by variability between locations. Elemental concentrations generally decreased outside the core, with some increase at age 3. The composition of the otolith core was very distinct from that of the other growth increments. In the Mediterranean, part of this differentiation was a result of Mn, which was present in the core at high concentrations compared with the rest of the otolith. Mediterranean otoliths also had higher concentrations of Sr, Zn and Ba in the core. For most samples a similar trend was observed, although samples from one of the Mediterranean areas showed some differences, mainly in the concentrations of Mg and Sr. These results provide new empirical evidence of the variation in elemental concentrations across hake otoliths with age, at least throughout the first 3 years of life.

Species boundaries in Zausodes-complex species (Copepoda:Harpacticoida:Harpacticidae) from the north-eastern Gulf of Mexico

2010 ◽  
Vol 24 (3) ◽  
pp. 258 ◽  
Author(s):  
Erin E. Easton ◽  
David Thistle ◽  
Trisha Spears
Keyword(s):  
New Species ◽  
Gulf Of Mexico ◽  
Harpacticoid Copepod ◽  
Taxonomic Revision ◽  
Rrna Gene ◽  
Complex Species ◽  
The North ◽  
Eastern Gulf Of Mexico ◽  
Mitochondrial Cytochrome B ◽  
North Eastern

Before 1999, the harpacticoid copepod species Zausodes arenicolus Wilson, 1932 was the subject of several ecological studies. Based on morphological evaluations of specimens from the north-eastern Gulf of Mexico, Bouck et al.’s (1999) taxonomic revision revealed three new species within what had been Z. arenicolus, making the ecological results suspect. Because harpacticoid copepods show convergent evolution and morphological stasis, Bouck et al.’s morphologically based approach may not have been sufficient. We have therefore re-evaluated their results using a combination of gene-sequencing and morphological methods. Partial mitochondrial cytochrome b (cytb) and 18S rRNA gene regions from individual Zausodes specimens found at five sites in the north-eastern Gulf of Mexico were separately analysed. Individuals of Zausodes septimus Lang, 1965 formed a single cytb gene-sequence clade, whereas Z. arenicolus individuals formed five. The uncorrected genetic divergences among these clades were 10 times those within them, providing evidence that each clade could be assigned to a different species. The 18S rDNA results support those from cytb. Subsequent morphological analysis revealed differences that will allow two clades of Z. arenicolus to be described as new species. Bouck et al. (1999) revealed some but not all of the species present.

The marketing of livestock in the North-Eastern Cape

Agrekon ◽  
1970 ◽  
Vol 9 (2) ◽  
pp. 9-13
Author(s):  
P. J.D. DU TOIT
Keyword(s):  
Eastern Cape ◽  
The North ◽  
North Eastern
Sign in / Sign up

Export Citation Format

Share Document