Evaluation of the wound-healing activity of Hibiscus rosa sinensis L (Malvaceae) in Wistar albino rats

Objective: The objective of the present research was to investigate the wound-healing potency of solid lipid nano particles of Hibiscus rosa sinensis extract. Crude herbal extracts and rudimentary formulations containing herbal extracts are good for demonstrating the feasibility of the concept; however, such formulations suffer with poor oral bioavailability and variability within groups of subjects. Converting herbal extracts into novel drug delivery systems may prove effective in addressing some of these problems. Methods: In the present study an attempt was made to develop Hibiscus rosa sinensis extract loaded solid lipid nanoparticles (HSLNs) using lipids glycerol monostearate (GMS) or beeswax. The prepared HSLNs were characterised for their size, surface charge and morphology. The optimized HSLNs were incorporated into Carbopol gel and tested for wound healing activity in male Wistar albino rats using excision wound model. Results: HSLNs of ~175 nm in size carrying negative charge were obtained with the optimised procedure using beeswax. The shape of the HSLNs was nearly spherical. The HSLNs (10 mg/ml) treated wounds healed much faster compared to raw crude extract and healing was comparable to marketed preparation. Conclusion: It is concluded that converting crude herbal extracts into SLNs can be an effective way to enhance the effectiveness of herbal extracts and their in vivo activity.

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EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

Kongunadu Research Journal ◽

10.26524/krj17 ◽

2014 ◽

Vol 1 (1) ◽

pp. 73-77

Author(s):

Uma Maheswari P ◽

Shalimol A ◽

Arumugasamy A ◽

Udhaya Sankar M.R

Keyword(s):

Wound Healing ◽

Dry Weight ◽

Wound Contraction ◽

Albino Rats ◽

Standard Drug ◽

Excision Wound ◽

Wound Model ◽

Wistar Albino Rats ◽

Incision Wound ◽

Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

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Inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in Wistar Albino rats

Journal of Materials Science Materials in Medicine ◽

10.1007/s10856-015-5543-y ◽

2015 ◽

Vol 26 (7) ◽

Cited By ~ 23

Author(s):

Renu Sankar ◽

Athmanathan Baskaran ◽

Kanchi Subramanian Shivashangari ◽

Vilwanathan Ravikumar

Keyword(s):

Wound Healing ◽

Copper Oxide ◽

Bacterial Growth ◽

Copper Oxide Nanoparticles ◽

Albino Rats ◽

Oxide Nanoparticles ◽

Excision Wound ◽

Wistar Albino Rats ◽

Wound Healing Activity

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Wound Healing Study of Panchavalkal Ointment in Wistar Albino Rats

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i36a31933 ◽

2021 ◽

pp. 110-116

Author(s):

Vaikhari Dhurve ◽

Pravin Jawanjal ◽

Mukesh Naria ◽

Tukaram Dudhamal ◽

Minal Kalambe ◽

...

Keyword(s):

Wound Healing ◽

Treated Group ◽

Control Group ◽

Albino Rats ◽

Wound Area ◽

Mean Values ◽

Wistar Strain ◽

The Mean ◽

Wistar Albino Rats ◽

Wound Healing Activity

Introduction: Panchavalkal is a well-known Ayurvedic poly-herbal formulation that has been reported to be used against inflammation, to clean ulcer, wound. Aims and Objectives: To investigate the wound healing activity of Panchavalkal ointment. Materials and Methods: Wistar strain albino rats of either sex weighing 200±20 g were used for the experiments divided in four groups each consisted of six rats. Statistical Analysis: One-way analysis of variance (ANOVA) was used to compare the mean values of quantitative variables among the groups followed by Dunnett’s multiple ‘t’ test Observations & Results: Sesame oil and Panchvalkal ointment showed almost similar wound healing effects in comparison to control group. Discussion: Panchavalkal ointment showed statistically highly significant percentage of contraction of excision wound area compared to the normal control. Epithelization period was significantly decreased in oil and Panchavalkal ointment treated group. Conclusion: Panchavalkal ointment decreased the pain, tenderness, redness and swelling that helps to control infection and enhanced the rate of wound healing in albino rats.

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