scholarly journals HIBISCUS ROSA SINENSIS LOADED SOLID LIPID NANOPARTICLES AND IN VIVO WOUND HEALING ACTIVITY IN WISTAR ALBINO RATS

2020 ◽  
pp. 78-83
Author(s):  
VIJAYANAND P. ◽  
JYOTHI V. ◽  
MOUNIKA A.
Keyword(s):  
Wound Healing ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Albino Rats ◽  
Herbal Extracts ◽  
Solid Lipid ◽  
Hibiscus Rosa Sinensis ◽  
Wistar Albino Rats ◽  
Wound Healing Activity

Objective: The objective of the present research was to investigate the wound-healing potency of solid lipid nano particles of Hibiscus rosa sinensis extract. Crude herbal extracts and rudimentary formulations containing herbal extracts are good for demonstrating the feasibility of the concept; however, such formulations suffer with poor oral bioavailability and variability within groups of subjects. Converting herbal extracts into novel drug delivery systems may prove effective in addressing some of these problems. Methods: In the present study an attempt was made to develop Hibiscus rosa sinensis extract loaded solid lipid nanoparticles (HSLNs) using lipids glycerol monostearate (GMS) or beeswax. The prepared HSLNs were characterised for their size, surface charge and morphology. The optimized HSLNs were incorporated into Carbopol gel and tested for wound healing activity in male Wistar albino rats using excision wound model. Results: HSLNs of ~175 nm in size carrying negative charge were obtained with the optimised procedure using beeswax. The shape of the HSLNs was nearly spherical. The HSLNs (10 mg/ml) treated wounds healed much faster compared to raw crude extract and healing was comparable to marketed preparation. Conclusion: It is concluded that converting crude herbal extracts into SLNs can be an effective way to enhance the effectiveness of herbal extracts and their in vivo activity.

Development of Topical Antibacterial Gel Loaded with Cefadroxil Solid Lipid Nanoparticles: In vivo Wound Healing Activity and Epithelialization Study

2020 ◽  
Vol 16 (4) ◽  
pp. 298-309
Author(s):  
Ehssan H. Moglad ◽  
Farhat Fatima ◽  
Mohammed Muqtader A ◽  
Vidya Devanathad ◽  
Md. Khalid Anw ◽  
...  
Keyword(s):  
Wound Healing ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Topical Antibacterial ◽  
Wound Healing Activity

scholarly journals Erratum to "Nanotechnology in Wound Healing; Semisolid Dosage Forms Containing Curcumin-Ampicillin Solid Lipid Nanoparticles, in Vitro, Ex Vivo and in Vivo Characteristics"

2020 ◽  
Vol 11 (1) ◽  
pp. 204-204
Author(s):  
Solmaz Ghaffari ◽  
Faezeh Alihosseini ◽  
Seyed Mahdi Rezayat Sorkhabadi ◽  
Sepideh Arbabi Bidgoli ◽  
Seyyedeh Elaheh Mousavi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Solid Lipid Nanoparticles ◽  
Ex Vivo ◽  
Lipid Nanoparticles ◽  
Dosage Forms ◽  
Solid Lipid

scholarly journals Development and Characterization of Solid Lipid Nanoparticles Containing Herbal Extract: In Vivo Antidepressant Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
P. Vijayanand ◽  
V. Jyothi ◽  
N. Aditya ◽  
A. Mounika
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Spherical Shape ◽  
Antidepressant Activity ◽  
Lipid Nanoparticles ◽  
Herbal Extract ◽  
Solid Lipid ◽  
Hibiscus Rosa Sinensis ◽  
Native Crude

In alternate systems of medicine like Ayurveda and traditional Chinese medicine, Hibiscus rosa sinensis and its extracts have been traditionally prescribed for their antidepressant activity. Crude extracts and rudimentary formulations approaches are good for proof-of-concept studies; however, these formulations are fraught with problems like poor oral bioavailability and high variability between subjects. Systematic drug delivery approaches could prove effective in addressing some of these problems. In this study, we report the development of Hibiscus rosa sinensis extract loaded solid lipid nanoparticles (HSLNs) using glycerol monostearate or beeswax as lipids. The HSLNs were evaluated for their size, surface charge, and morphology. The optimized HSLNs were tested for antidepressant activity in male Swiss albino mice. It was found that, with the optimized procedure, HSLNs of ~175 nm, carrying negative charge and nearly spherical shape, could be obtained. The in vivo test results suggested that there were marked differences in the immobility times of the test animals. Moreover, with HSLNs, it was found that at doses several times lower than the native crude extract dose, similar pharmacological effect could be obtained. These initial findings suggest that encapsulating phytopharmaceuticals into advanced delivery systems like solid lipid nanoparticles can be an effective strategy in improving their in vivo performance.

99mTc(I) carbonyl-radiolabeled lipid based drug carriers for temozolomide delivery and bioevaluation by in vitro and in vivo

2019 ◽  
Vol 107 (12) ◽  
pp. 1185-1193
Author(s):  
Kadir Arı ◽  
Eser Uçar ◽  
Çiğdem İçhedef ◽  
Ayfer Yurt Kılçar ◽  
Emin İlker Medine ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Drug Carriers ◽  
Lipid Nanoparticles ◽  
Biological Behavior ◽  
Albino Rats ◽  
Target Tissue ◽  
Preclinical Research ◽  
Solid Lipid

Abstract In preclinical research radiolabeled nanoparticles have been attracting interest as a new class of imaging probes. Assuming good stability of solid lipid nanoparticles (SLNs) under physiological conditions, radiolabeled SLNs can be used for imaging and measuring uptake in target tissue. Present study was performed to evaluate biological behavior of temozolomide (TMZ) loaded solid lipid nanoparticles (SLN-TMZ) in vivo and in vitro. Lipid nanoparticles were prepared by emulsification and low-temperature solidification method. ζ potential, morphology and particle size of nanoparticles were determined. Biological behavior of 99mTc(CO)3+ radiolabeled SLN-TMZ were investigated in vitro on U87/Daoy cell lines and in vivo on female Wistar Albino rats. Obtained results of in vitro incorporation, in vivo biodistribution and gamma imaging studies on radiolabeled SLN-TMZ show that the radiolabeled solid lipid nanoparticles could have potential as a drug delivery system for TMZ.

Retinoic acid-loaded solid lipid nanoparticles surrounded by chitosan film support diabetic wound healing in in vivo study

2020 ◽  
Vol 188 ◽  
pp. 110749 ◽  
Author(s):  
Valquíria T. Arantes ◽  
André A.G. Faraco ◽  
Frederico B. Ferreira ◽  
Cleida A. Oliveira ◽  
Elisângela Martins-Santos ◽  
...  
Keyword(s):  
Wound Healing ◽  
Retinoic Acid ◽  
Solid Lipid Nanoparticles ◽  
Chitosan Film ◽  
Lipid Nanoparticles ◽  
In Vivo Study ◽  
Diabetic Wound ◽  
Solid Lipid ◽  
Diabetic Wound Healing

scholarly journals Key features of Solid Lipid Nanoparticles prepared with nanoclay and spring water ingredients with demonstrated wound healing activity: a pilot study

2020 ◽  
Author(s):  
Fátima García-Villén ◽  
Rita Sánchez-Espejo ◽  
Ana Borrego-Sánchez ◽  
Pilar Cerezo ◽  
Raquel de Melo Barbosa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Pilot Study ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Spring Water ◽  
Solid Lipid ◽  
Key Features ◽  
Wound Healing Activity

scholarly journals Nanotechnology in Wound Healing; Semisolid Dosage Forms Containing Curcumin-Ampicillin Solid Lipid Nanoparticles, in-Vitro, Ex-Vivo and in-Vivo Characteristics

2018 ◽  
Vol 8 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Solmaz Ghaffari ◽  
Faezeh Alihosseini ◽  
Seyed Mahdi Rezayat Sorkhabadi ◽  
Sepideh Arbabi Bidgoli ◽  
Seyyedeh Elaheh Mousavi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Solid Lipid Nanoparticles ◽  
Ex Vivo ◽  
Lipid Nanoparticles ◽  
Dosage Forms ◽  
Solid Lipid

Pharmacokinetic Evaluation of 99mTc-radiolabeled Solid Lipid Nanoparticles and Chitosan Coated Solid Lipid Nanoparticles

2020 ◽  
Vol 20 (13) ◽  
pp. 1044-1052
Author(s):  
Nasrin Abbasi Gharibkandi ◽  
Sajjad Molavipordanjani ◽  
Jafar Akbari ◽  
Seyed Jalal Hosseinimehr
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
High Resistivity ◽  
Scanning Calorimetry ◽  
Liver Uptake ◽  
Solid Lipid ◽  
Transmission Electron ◽  
Main Portion ◽  
Pharmacokinetic Behavior

Background: Solid Lipid Nanoparticles (SLNs) possess unique in vivo features such as high resistivity, bioavailability, and habitation at the target site. Coating nanoparticles with polymers such as chitosan greatly affects their pharmacokinetic behavior, stability, tissue uptake, and controlled drug delivery. The aim of this study was to prepare and evaluate the biodistribution of 99mTc-labeled SLNs and chitosan modified SLNs in mice. Methods: 99mTc-oxine was prepared and utilized to radiolabel pre-papered SLNs or chitosan coated SLNs. After purification of radiolabeled SLNs (99mTc-SLNs) and radiolabeled chitosan-coated SLNs (99mTc-Chi-SLNs) using Amicon filter, they were injected into BALB/c mice to evaluate their biodistribution patterns. In addition, nanoparticles were characterized using Transmission Electron Microscopy (TEM), Fourier-transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), X-ray Powder Diffraction (XRD) and Dynamic Light Scattering (DLS). Results: 99mTc-oxine with high radiochemical purity (RCP~100%) and stability (RCP > 97% at 24 h) was used to provide 99mTc-SLNs and 99mTc-Chi-SLNs with high initial RCP (100%). TEM image and DLS data suggest 99mTc- SLNs susceptibility to aggregation. To that end, the main portion of 99mTc-SLNs radioactivity accumulates in the liver and intestines, while 99mTc-Chi-SLNs sequesters in the liver, intestines and kidneys. The blood radioactivity of 99mTc-Chi-SLNs was higher than that of 99mTc-SLNs by 7.5, 3.17 and 3.5 folds at 1, 4 and 8 h post-injection. 99mTc- Chi-SLNs uptake in the kidneys in comparison with 99mTc-SLNs was higher by 37.48, 5.84 and 11 folds at 1, 4 and 8h. Conclusion: The chitosan layer on the surface of 99mTc-Chi-SLNs reduces lipophilicity in comparison with 99mTc- SLNs. Therefore, 99mTc-Chi-SLNs are less susceptible to aggregation, which leads to their lower liver uptake and higher kidney uptake and blood concentration.

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