scholarly journals Association of platelet count and serological markers of dengue infection- importance of NS1 antigen

2011 ◽  
Vol 29 (4) ◽  
pp. 359 ◽  
Author(s):  
RD Kulkarni ◽  
GS Ajantha ◽  
AS Kalabhavi ◽  
PC Shetty ◽  
SS Patil ◽  
...  
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Serological Markers ◽  
Ns1 Antigen

STUDY OF NS1 ANTIGEN DETECTION AND ASSOCIATION OF PLATELET COUNT WITH VARIOUS DENGUE SEROLOGICAL MARKERS IN DENGUE INFECTION: AN INSTITUTIONAL EXPERIENCE"

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Neelam Gupta ◽  
Rahul Kumar Goyal
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Dengue Shock Syndrome ◽  
Accurate Diagnosis ◽  
Serological Markers ◽  
Ns1 Protein ◽  
New Approach ◽  
Ns1 Antigen ◽  
Institutional Experience ◽  
Initiation Of Therapy

Introduction: Dengue is a mosquito-borne disease caused by dengue virus. Annually, it affects up millions of people worldwide. An early and accurate diagnosis of dengue in the acute phase of illness is important for identifying an epidemic and for initiation of therapy. Detection of the secreted NS1 protein is a new approach that aid in the early diagnosis. Platelet count is the only non-dengue parameter that can support the diagnosis of the dengue shock syndrome and dengue. This study was done to detect dengue parameters and correlate them with the platelet count. Material and Methods: This study was conducted from September 2016 to November 2016. Total 205 blood samples were collected from clinically suspected dengue cases. Serum was separated and tested for NS1 antigen, IgM and IgG using the immunochromatography kit. The platelet count was also recorded in all samples. Results: Out of 205 samples tested total 92(44.9%) specimens were positive for one or more dengue parameters. Among 92 specimens 45(49%) were positive for only NS1 antigen and 20 were positive for IgM only. Thrombocytopenia was recorded in 73(79%)out of 92 dengue positive cases. Conclusion: Inclusion of NS1 in the diagnosis of dengue increases the detection rate significantly in early phase of the infection.

A PROSPECTIVE STUDY ON VARIOUS SEROLOGICAL MARKERS AND PLATELET COUNT IN DENGUE INFECTION IN AND AROUND KANCHEEPURAM.

2021 ◽  
pp. 45-46
Author(s):  
Hamsadwani K P ◽  
Sneka P ◽  
Sangamithra V
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Febrile Illness ◽  
Antibody Detection ◽  
Serological Markers ◽  
Serum Samples ◽  
Platelet Counts ◽  
Acute Viral Infection ◽  
Ns1 Antigen ◽  
A Prospective Study

INTRODUCTION: Dengue is an acute viral infection with potential fatal complications, presenting with non-specic fever that mimics other febrile illness. Specic antibody detection has been the mainstay of diagnosis which is prone for false positive and negative reactions. The newer parameter NS1 appears to be highly specic and reliable for diagnosis. Therefore, we tried to evaluate the association of platelet counts against NS1 and IgM / IgG in dengue infections. OBJECTIVE: To study the association of thrombocytopenia with NS1 antigen and specic antibodies in dengue infection in kancheepuram population. MATERIALS AND METHODS: Serum samples from clinically suspected dengue cases were tested for NS1,IgM,IgG by immunochromatography – based test. Platelet counts were obtained from all positive cases. Test results of dengue-specic parameters and platelet counts were compared. RESULTS: Of the 612 samples tested, 150 were positive for one or more dengue serological markers. Of the 150, 27(18%) were positive for NS1only, 45(30%)were positive for IgM, 54(36%) were positive for IgG only. Of the 150, 39(26%) showed platelet count less than 1 lakh. CONCLUSION: Inclusion of NS1 in the diagnosis of dengue increases the detection and specicity rate. Thus correlation of platelet count, NS1,IgM and ,IgG gives detailed picture of the stage and prognosis of the disease

scholarly journals Evaluation of Some Hematological and Serological Changes in Dengue Patients of Lahj-Yemen

2020 ◽  
Vol 1 (1) ◽  
pp. 25-29
Author(s):  
Manal Mohamed AL-Samadi ◽  
Khaled Saeed Ali
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Igg Antibodies ◽  
Igm Antibodies ◽  
Ns1 Antigen ◽  
Wbc Count

The present study aimed to evaluate the changes in WBC and platelet count and detection of NS1 antigen, IgM and IgG antibodies in dengue patients. There were 103 confirmed cases of dengue including 49(47.6%) females and 54(52.4%) males. The WBC count was less than (2000 /μl) in 16 cases and was ranging between (2000-4000 /μl) in 44 cases. The platelets count decreased to <50000/μl in 4 (3.9%) of cases, <100,000/μl (50,000- 100,000/μl) in 14 (13.6%) of cases, and were between (100000-150000/ul) in 12 cases. About 83 (80.6%) patients were positive NS1 antigen, and 4 (3.9%) patients had IgM antibodies against dengue. Remaining 16 (15.5%) had both IgM and IgG antibodies against dengue. The identification of NS1 antigen, WBC and platelet count can be used as predictors of dengue infection.

DENGUE FEVER: CLINICO-PATHOLOGICAL CORRELATION WITH SEROLOGICAL MARKERS AND PLATELET COUNT

2021 ◽  
pp. 1-2
Author(s):  
Gunjan Jain ◽  
Saloni Savani ◽  
Hardik Jain
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Rna Virus ◽  
Dengue Infection ◽  
White Blood Cells ◽  
Hemorrhagic Fever ◽  
Serological Markers ◽  
Platelet Counts ◽  
Microbiological Laboratory ◽  
Viral Illness

Dengue is an acute viral illness caused by RNA virus of the family Flaviviridae and spread by Aedes mosquitoes. Presenting features may range from asymptomatic fever to dreaded complications such as hemorrhagic fever and shock. A decreased number of white blood cells (leukopenia) accompanied by a decreased number of platelet count (thrombocytopenia) and metabolic acidosis are the initial changes on laboratory examinations. Microbiological laboratory testing conrms the diagnosis of Dengue Fever. Virus segregation in cell cultures, nucleic acid demonstration by polymerase chain reaction (PCR), and serological detection of viral antigens (such as NS1) or particular antibodies are the preferred microbiological assays. This study focuses on the importance of platelet counts and other platelet parameters in dengue infection. Also platelet counts are compared with clinical, radiological and other serological markers.

scholarly journals Serum Ferritin: A Backstage Weapon in Diagnosis of Dengue Fever

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Soumyabrata Roy Chaudhuri ◽  
Subhayan Bhattacharya ◽  
Mainak Chakraborty ◽  
Kingshuk Bhattacharjee
Keyword(s):  
Platelet Count ◽  
Retrospective Study ◽  
Dengue Fever ◽  
Serum Ferritin ◽  
Ferritin Level ◽  
Dengue Infection ◽  
Surrogate Marker ◽  
Febrile Illness ◽  
Dengue Outbreak ◽  
Ns1 Antigen

Aims. This retrospective study evaluates ferritin as a surrogate marker for dengue infection (NS1 and IgM negative stage) as opposed to other febrile illnesses of infective or inflammatory etiology (OFI). Methodology. Data of all patients admitted to medical ward and medical ITU during the dengue outbreak were collected. Patients admitted between 5 and 10 days of febrile illness without a diagnosis were included. Patients with NS1 positivity (Days 2–8) and/or positive IgM for dengue (Days 6–10) were considered to be dengue cases and those with other confirmed diagnoses were considered in the OFI group. Ferritin, CRP, TC of WBC, platelet count, SGOT, SGPT, and albumin levels were analysed for both groups. Results. We examined 30 cases of clinically and serologically confirmed dengue fever and 22 cases of OFI. Ferritin level in dengue cohort was significantly higher than the OFI group (p<0.0001). The best cut-off for ferritin level to differentiate dengue from OFI was found to be 1291. The sensitivity at this cut-off is 82.6% and the specificity at this cut-off is 100%. Conclusion. Ferritin may serve as a significant marker for differentiating between dengue fever and OFI, in absence of a positive NS1 antigen or a positive IgM antibody for dengue.

scholarly journals Trend of Dengue Virus Infection with Seasonal Variation at HIMS, Varanasi, Uttar Pradesh, India

2021 ◽  
Author(s):  
Hari Om Trivedi ◽  
Asim Kumar Singh ◽  
Mukesh Kumar Singh
Keyword(s):  
Platelet Count ◽  
Seasonal Variation ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Uttar Pradesh ◽  
Febrile Illness ◽  
Cerebral Hypoperfusion ◽  
Structural Protein ◽  
Igg Antibody ◽  
Ns1 Antigen

Introduction: Dengue is the most common mosquito borne arboviral infection in India, and it has become endemic in India with consistent episodes. Dengue is an acute febrile illness caused by Dengue Virus (DENV-1, DENV-2, DENV-3, DENV-4), an arthropod of family Flaviviridae, transmitted through the bite of female Aedes aegypti mosquitoes. Although, dengue is more prevalent in humans but also it may sometimes be seen in monkeys. The only animals to be affected when bitten by a mosquito, infected with dengue fever are monkeys other animals do not carry or spread dengue. Patient presents with hyperthermia, headache, severe joint and muscle pain, fatigue and skin rashes. Neurological manifestation in sever dengue results from multiorgan dysfunction due to cerebral edema, haemorrhage due to vascular leak, cerebral hypoperfusion, and electrolyte disturbances. Aim: To study serological markers with association of platelet count and trend of DENV infection with seasonal variation. Materials and Methods: The present retrospective study was conducted for a period of two years in Heritage Institute of Medical Sciences (HIMS), Varanasi, Uttar Pradesh, India, from January 2017 to December 2018. Blood samples were collected for 2140 suspected dengue patients. Out of the serologically positive cases, serological confirmation and platelet count was done for dengue infection. Data was presented as percentages. Results: Total 2140 suspected cases were admitted and out of them 199 (9.3%) were found to be seropositive for dengue infection. Non structural protein 1 (NS1) positive cases out of which total seropositive cases were reported to be 127 (63.81%) and 147 (73.86%) cases were positive for NS1 antigen either alone or along with antibody. Out of the total 199 positive cases thrombocytopenia was observed in 126 (63.31%) cases. Positive cases which were under 15 years of age were reported to be 92 (46.23%). Conclusion: The study concludes that NS1 antigen and IgM- IgG antibody consideration in the diagnosis of dengue infection builds the opportunity of early diagnosis so as to keep away the complications significantly.

scholarly journals Analysis of Platelet Indices and its correlation with platelet count In Dengue

2020 ◽  
Vol 11 (SPL2) ◽  
pp. 284-289
Author(s):  
Dhanya Menon ◽  
Ganthimathy Sekhar ◽  
Siddharth A R ◽  
Sridevi M
Keyword(s):  
Platelet Count ◽  
Dengue Infection ◽  
Large Cell ◽  
Health Concern ◽  
P Value ◽  
Distribution Width ◽  
Platelet Indices ◽  
Ns1 Antigen ◽  
Automated Hematology Analyzer

Dengue is a global arboviral disease of growing public health concern. Several parameters have been used to detect the infection and severity of dengue. Recent evidence suggests that platelet indices can be used to assess the severity and prognosis of dengue infection. This retrospective study aims at assessing the role of the platelet indices – Mean Platelet Volume (MPV), Platelet Distribution Width, (PDW), P-LCR (Platelet Large Cell Ratio) and Plateletcrit (PCT) and its correlation with the platelet count in 50 Dengue NS1 antigen positive cases and to find the sex ratio and the age-wise distribution of the dengue cases. The indices were obtained using an Automated Hematology analyzer and were compared with the platelet count, considering p-value <0.05 as statistically significant. Thrombocytopenia was found in a majority of the cases and PDW and PLCR were found to be inversely proportional to the platelet count. MPV was found to have no correlation with the platelet count. Hence, we conclude that Platelet count, PDW, and P-LCR can be used as predictors of Dengue infection and severity, whereas MPV cannot be used as a predictor of Dengue infection or severity.

Sign in / Sign up

Export Citation Format

Share Document