Correlation of thrombocytopenia and serological markers in early diagnosis of dengue infection with special reference to NS1 antigen

Introduction: Dengue is a mosquito-borne disease caused by dengue virus. Annually, it affects up millions of people worldwide. An early and accurate diagnosis of dengue in the acute phase of illness is important for identifying an epidemic and for initiation of therapy. Detection of the secreted NS1 protein is a new approach that aid in the early diagnosis. Platelet count is the only non-dengue parameter that can support the diagnosis of the dengue shock syndrome and dengue. This study was done to detect dengue parameters and correlate them with the platelet count. Material and Methods: This study was conducted from September 2016 to November 2016. Total 205 blood samples were collected from clinically suspected dengue cases. Serum was separated and tested for NS1 antigen, IgM and IgG using the immunochromatography kit. The platelet count was also recorded in all samples. Results: Out of 205 samples tested total 92(44.9%) specimens were positive for one or more dengue parameters. Among 92 specimens 45(49%) were positive for only NS1 antigen and 20 were positive for IgM only. Thrombocytopenia was recorded in 73(79%)out of 92 dengue positive cases. Conclusion: Inclusion of NS1 in the diagnosis of dengue increases the detection rate significantly in early phase of the infection.

Download Full-text

P1037 Evaluation of Platelia™ Dengue NS1 Antigen assay for early diagnosis of acute dengue infection

International Journal of Antimicrobial Agents ◽

10.1016/s0924-8579(07)70878-6 ◽

2007 ◽

Vol 29 ◽

pp. S278

Author(s):

M. Tabouret ◽

C. Salanon ◽

P. Sarfati

Keyword(s):

Early Diagnosis ◽

Dengue Infection ◽

Antigen Assay ◽

Ns1 Antigen

Download Full-text

Burden of Dengue and Chikungunya – A Retrospective Study

Journal of Pure and Applied Microbiology ◽

10.22207/jpam.15.2.30 ◽

2021 ◽

Author(s):

J.V. Sathish ◽

Mita D. Wadekar ◽

S. Jayashree ◽

C. Pooja

Keyword(s):

Retrospective Study ◽

Early Diagnosis ◽

Dengue Infection ◽

Control Measures ◽

Enzyme Linked Immunosorbent Assay ◽

Age Group ◽

Capture Elisa ◽

Igm Antibody ◽

Ns1 Antigen ◽

And Control

Arboviral infections like dengue fever and chikungunya are the most common infections that share the same Aedes mosquito vectors. Clinical presentations of these two infections are also similar, especially in initial stages. Non-structural antigen (NS1 Ag)detection for dengue and detection of IgM antibodies by capture ELISA for chikungunya and dengue infection may help in the early diagnosis. Early diagnosis is essential for the treatment and control measures. The present study was conducted to know the burden of dengue and chikungunya. A retrospective study was conducted for a period of 1 year from Dec 2017 to Nov 2018 to know the burden of dengue and chikungunya in Chamarajanagar. Dengue (> 5 days fever) and chikungunya testing was done by IgM antibody capture ELISA kits produced by NIV. Dengue samples (< 5 days fever) were subjected to NS1 antigen detection by microwell enzyme-linked immunosorbent assay (ELISA) from Qualpro diagnostics. The tests were carried out following manufacturer’s instruction. Samples received for dengue NS1 Ag testing was 446, of which, 49(11.0%) were positive and of 730 samples received for IgM antibody, 53 (7.3%) were positive. Age group commonly affected was 0-20 years 44(43.1%). Of 668 samples received for chikungunya test, 86 (12.9%) were positive. Maximum number of cases was seen in age group of 21-40 years 45(52.3%). Males 56(54.9%) were affected higher than female 46(45.1%) in dengue infection while in chikungunya, females 45(52.3%) were more affected than males 41(47.7%). Both infections are high in the month of June and July. Early detection of dengue by NS1 antigen and detection of Ig M antibodies by capture ELISA chikungunya and dengue infection helps in appropriate treatment and initiation of prevention and control measures by community awareness and vector control.

Download Full-text

THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i11.720 ◽

2019 ◽

Vol 3 (11) ◽

Author(s):

Dr. Tanajee Zade ◽

Dr. K. Srinivas ◽

Dr. Akshay Berad

Keyword(s):

Early Diagnosis ◽

Dengue Fever ◽

Dengue Infection ◽

Tertiary Care ◽

High Morbidity ◽

Care Hospital ◽

Serum Samples ◽

Igg Antibody ◽

Igm Antibody ◽

Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words: Dengue, NS1 Antigen, IgM antibody, Platelet

Download Full-text