scholarly journals Hydrogen-rich Water Exerting a Protective Effect on Ovarian Reserve Function in a Mouse Model of Immune Premature Ovarian Failure Induced by Zona Pellucida 3

2016 ◽  
Vol 129 (19) ◽  
pp. 2331-2337 ◽  
Author(s):  
Xin He ◽  
Shu-Yu Wang ◽  
Cheng-Hong Yin ◽  
Tong Wang ◽  
Chan-Wei Jia ◽  
...  
Keyword(s):  
Mouse Model ◽  
Protective Effect ◽  
Zona Pellucida ◽  
Premature Ovarian Failure ◽  
Ovarian Reserve ◽  
Ovarian Failure

Autoimmunity to zona pellucida possibly causes Premature Ovarian Failure (POF)

2006 ◽  
Vol 71 (2) ◽  
pp. 155 ◽  
Author(s):  
A. Kinoshita ◽  
H. Tanaka ◽  
S. Komori ◽  
A. Hasegawa ◽  
K. Koyama
Keyword(s):  
Zona Pellucida ◽  
Premature Ovarian Failure ◽  
Ovarian Failure

scholarly journals Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation

2018 ◽  
Vol 51 (5) ◽  
pp. 2341-2358 ◽  
Author(s):  
Xiaowei Nie ◽  
Youjin Dai ◽  
Yuan Zheng ◽  
Dan Bao ◽  
Qin Chen ◽  
...  
Keyword(s):  
Mouse Model ◽  
Premature Ovarian Failure ◽  
Cell Apoptosis ◽  
Quantitative Polymerase Chain Reaction ◽  
Oocyte Quality ◽  
Ovarian Failure ◽  
Control Group ◽  
Mrna Levels ◽  
Primordial Follicles ◽  
Ovarian Aging

Background/Aims: This study investigated the effect of consecutive superovulation on the ovaries and established a premature ovarian failure (POF) model in mice. Methods: The mouse POF model was induced by 5-15 consecutive superovulation treatments with pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) and prostaglandin F2α (PGF2α). Normal adult mice were compared with mice displaying natural ovarian aging. The following serum biochemical parameters were measured: including follicle-stimulating hormone (FSH), luteinizing hormone (LH), progesterone (P), estradiol (E2), inhibin B (INH B), malondialdehyde (MDA), total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels. Follicles were counted using H&E staining. Levels of 8-hydroxyguanosine (8-OhdG), 4-hydroxynonenal (4-HNE), nitrotyrosine (NTY), anti-Mullerian hormone (AMH) and CDKN2A/ p16 (p16) were detected using immunohistochemical staining. Reactive oxygen species (ROS) levels were measured using dihydroethidium (DHE) staining. Cell apoptosis was detected using an in situ TUNEL fluorescence staining assay. Levels of proteins involved in ROS-related pathways and the p16 protein were detected using Western blotting. Sod1, Sod2 and Sod3 mRNA levels were detected using quantitative polymerase chain reaction (Q-PCR). Oocyte quality was evaluated using in vitro fertilization (IVF) and zygote culture. Results: Consecutive superovulation groups presented lower P, E2, SOD, GSH-Px and INH B levels, significantly higher FSH, LH, MDA and ROS levels, and significantly fewer primordial follicles compared with the control group. Consecutive superovulation groups presented significantly increased levels of Sod2, 8-OhdG, 4-HNE, NTY, significantly increased levels of the SIRT1 and FOXO1 proteins, significantly increased levels of the senescence-associated protein p16, as well as decreased AMH, Sod1 and Sod3 levels and increased granulosa cell apoptosis compared with the control group. Conclusion: Consecutive superovulation significantly decreased ovarian function and oocyte quality and increased oxidative stress and apoptosis in the ovary via a mechanism involving the p16 and SIRT1/FOXO1 signaling pathways. These findings suggest that consecutive superovulation may be used to establish a mouse model of ovarian aging.

The protective effect of fenofibrate, triptorelin, and their combination against premature ovarian failure in rats

2020 ◽  
Vol 394 (1) ◽  
pp. 137-149
Author(s):  
Walaa Yehia Abdelzaher ◽  
Sara Mohammed Naguib Abdel-Hafez ◽  
Remon Roshdy Rofaeil ◽  
Abdel Hamid Sayed AboBakr Ali ◽  
AbdelRahman Hegazy ◽  
...  
Keyword(s):  
Protective Effect ◽  
Premature Ovarian Failure ◽  
Ovarian Failure

scholarly journals Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival

2019 ◽  
Vol 20 (14) ◽  
pp. 3605 ◽  
Author(s):  
Yu Jiang ◽  
Zhaoyuan Zhang ◽  
Lijun Cha ◽  
Lili Li ◽  
Dantian Zhu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Signaling Pathway ◽  
Premature Ovarian Failure ◽  
Ovarian Function ◽  
Ovarian Failure ◽  
Productive Capacity ◽  
Tnf Α ◽  
Hh Signaling ◽  
Female Germline

This study was designed to investigate the protective effect of resveratrol (RES) on premature ovarian failure (POF) and the proliferation of female germline stem cells (FGSCs) at the tissue and cell levels. POF mice were lavaged with RES, and POF ovaries were co-cultured with RES and/or GANT61 in vitro. FGSCs were pretreated with Busulfan and RES and/or GANT61 and co-cultured with M1 macrophages, which were pretreated with RES. The weights of mice and their ovaries, as well as their follicle number, were measured. Ovarian function, antioxidative stress, inflammation, and FGSCs survival were evaluated. RES significantly increased the weights of POF mice and their ovaries as well as the number of follicles, while it decreased the atresia rate of follicles. Higher levels of Mvh, Oct4, SOD2, GPx, and CAT were detected after treatment with RES in vivo and in vitro. RES treatment resulted in significantly lower TNF-α and IL-6 concentrations and an obviously higher IL-10 concentration in the ovaries. In FGSCs, higher Mvh, Oct4, and SOD2 concentrations and lower TNF-α, IL-6, and MDA concentrations were measured in the RES group. Blockage of the Hh signaling pathway reversed the protective effect of RES on FGSCs. In conclusion, RES effectively improved the ovarian function of the POF model and the productive capacity of FGSCs via relieving oxidative stress and inflammation and a mechanism involving the Hh signaling pathway, suggesting that RES is a potential agent against POF and can aid in the survival of FGSCs.

scholarly journals Premature Ovarian Failure in Mice with Oocytes Lacking Core 1-Derived O-Glycans and Complex N-Glycans

Endocrinology ◽  
2011 ◽  
Vol 152 (3) ◽  
pp. 1057-1066 ◽  
Author(s):  
Suzannah A. Williams ◽  
Pamela Stanley
Keyword(s):  
Mouse Model ◽  
Premature Ovarian Failure ◽  
Double Mutant ◽  
Ovarian Function ◽  
Ovarian Failure ◽  
Feedback Loops ◽  
Primary Follicle ◽  
Inhibin A ◽  
Small Litter ◽  
Specific Deletion

Premature ovarian failure (POF) affects up to 1.4% of women under the age of 40 yr and less than 30% of cases have a known cause. Here we describe a new mouse model of POF resulting from oocyte-specific ablation of core 1-derived (mucin) O-glycans and complex and hybrid N-glycans. Females carrying floxed alleles of both the C1galt1 (T-syn) and Mgat1 glycosyltransferase genes and a ZP3Cre transgene, generate oocytes lacking complex O- and N-glycans following oocyte-specific deletion at the primary follicle stage. We previously showed that few double-mutant females are fertile, and those produce only a single small litter. Here we show that ovarian function declined rapidly in double-mutant females with less than 1% ovulating at 11 wk of age after superovulation with exogenous gonadotropins. Ovary weight was significantly decreased in double-mutant females by 3 months of age, consistent with a decrease in the number of developing follicles. FSH levels in double-mutant females were elevated at 3 months of age, and testosterone and inhibin A were decreased, showing that the loss of complex N- and O-glycans from oocyte glycoproteins affected hypothalamic-pituitary-gonadal feedback loops. The absence of developing follicles, ovary dysfunction, reduced testosterone and inhibin A, and elevated FSH in double-mutant females lacking C1galt1 and Mgat1 in oocytes represents a new mouse model for the study of follicular POF.

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