Genomic analysis and clinical importance of Escherichia coli isolate from patients with sepsis

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harbouring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harbouring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38 and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbour resistance genes. The blaOXA-244 gene was chromosomally-encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. For our knowledge this is the first report of a blaOXA-244–harbouring Escherichia coli isolate in American continent.Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently on the American continent.

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Comparative Genomic Analysis Shows That Avian Pathogenic Escherichia coli Isolate IMT5155 (O2:K1:H5; ST Complex 95, ST140) Shares Close Relationship with ST95 APEC O1:K1 and Human ExPEC O18:K1 Strains

PLoS ONE ◽

10.1371/journal.pone.0112048 ◽

2014 ◽

Vol 9 (11) ◽

pp. e112048 ◽

Cited By ~ 40

Author(s):

Xiangkai Zhu Ge ◽

Jingwei Jiang ◽

Zihao Pan ◽

Lin Hu ◽

Shaohui Wang ◽

...

Keyword(s):

Escherichia Coli ◽

Genomic Analysis ◽

Comparative Genomic Analysis ◽

Comparative Genomic ◽

Avian Pathogenic Escherichia Coli ◽

Coli Isolate ◽

Pathogenic Escherichia Coli ◽

Close Relationship

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First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent

Antibiotics ◽

10.3390/antibiotics8040222 ◽

2019 ◽

Vol 8 (4) ◽

pp. 222 ◽

Cited By ~ 3

Author(s):

Deisy Abril ◽

Ingrid Gisell Bustos Moya ◽

Ricaurte Alejandro Marquez-Ortiz ◽

Diego Fernando Josa Montero ◽

Zayda Lorena Corredor Rozo ◽

...

Keyword(s):

Escherichia Coli ◽

Comparative Genomics ◽

Genomic Island ◽

Genomic Analysis ◽

American Continent ◽

Incompatibility Group ◽

Coli Isolate ◽

First Report ◽

E Coli ◽

Comparative Genomics Analysis

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The blaOXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a blaOXA-244–harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.

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“Effect of Subinhibitory Concentrations of Some Antibiotics and Low Doses of Gamma Radiation on the Cytotoxicity and Expression of Colibactin by an Uropathogenic Escherichia coli isolate”

Current Microbiology ◽

10.1007/s00284-020-02331-6 ◽

2021 ◽

Author(s):

Radwa N. Morgan ◽

Hala A. Farrag ◽

Mohammad M. Aboulwafa ◽

Sarra E. Saleh

Keyword(s):

Escherichia Coli ◽

Gamma Radiation ◽

Uropathogenic Escherichia Coli ◽

Low Doses ◽

Coli Isolate ◽

Subinhibitory Concentrations

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Coexistence of fluorescent Escherichia coli strains in millifluidic droplets reactors

Lab on a Chip ◽

10.1039/d0lc01204a ◽

2021 ◽

Author(s):

Xinne Zhao ◽

Rico Illing ◽

Philip Ruelens ◽

Michael Bachmann ◽

Gianaurelio Cuniberti ◽

...

Keyword(s):

Escherichia Coli ◽

Clinical Importance ◽

Competition And Cooperation ◽

Species Evolution

Understanding competition and cooperation within microbiota is of high fundamental and clinical importance, helping to comprehend species’ evolution and biodiversity. We co-encapsulated and cultured two isogenic Escherichia coli strains expressing...

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