scholarly journals Pattern of drug resistance of Mycobacterium tuberculosis clinical isolates to first-line antituberculosis drugs in pulmonary cases

Lung India ◽  
2015 ◽  
Vol 32 (4) ◽  
pp. 339 ◽  
Author(s):  
Surya Kant ◽  
Kanchan Srivastava ◽  
AjayK Sharma ◽  
Deepika Kalo
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Clinical Isolates ◽  
First Line ◽  
Antituberculosis Drugs

scholarly journals In Vitro Activities of Six Fluoroquinolones against 250 Clinical Isolates of Mycobacterium tuberculosisSusceptible or Resistant to First-Line Antituberculosis Drugs

2000 ◽  
Vol 44 (9) ◽  
pp. 2567-2568 ◽  
Author(s):  
María Jesús Ruiz-Serrano ◽  
Luis Alcalá ◽  
Lucía Martínez ◽  
Marisol Díaz ◽  
Mercedes Marín ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
Good Activity ◽  
In Vitro Activity ◽  
First Line ◽  
Antituberculosis Drugs

ABSTRACT Two hundred fifty isolates of Mycobacterium tuberculosis were evaluated for susceptibility to ciprofloxacin, ofloxacin, levofloxacin, grepafloxacin, trovafloxacin, and gemifloxacin (SB-265805). Levofloxacin, ciprofloxacin, and grepafloxacin showed the greatest activity (MIC for 90% of strains tested [MIC90] 1 μg/ml), although ofloxacin also showed good activity, with an MIC90 of 2 μg/ml. Trovafloxacin and gemifloxacin showed lower in vitro activity, with MIC90s of 64 and 8 μg/ml, respectively.

scholarly journals Resazurin Microtiter Assay Plate: Simple and Inexpensive Method for Detection of Drug Resistance in Mycobacterium tuberculosis

2002 ◽  
Vol 46 (8) ◽  
pp. 2720-2722 ◽  
Author(s):  
Juan-Carlos Palomino ◽  
Anandi Martin ◽  
Mirtha Camacho ◽  
Humberto Guerra ◽  
Jean Swings ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Inexpensive Method ◽  
Antituberculosis Drugs ◽  
Specificity And Sensitivity ◽  
Proportion Method ◽  
Microtiter Assay

ABSTRACT A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

scholarly journals Diversified lineages and drug-resistance profiles of clinical isolates of Mycobacterium tuberculosis complex in Malaysia

2019 ◽  
Vol 8 (4) ◽  
pp. 320
Author(s):  
MohdSalleh Zaki ◽  
MohdNur Noorizhab Fakhruzzaman ◽  
NorzulianaZainal Abidin ◽  
ZirwatulAdilah Aziz ◽  
WaiFeng Lim ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Clinical Isolates ◽  
Tuberculosis Complex

scholarly journals Evaluation of Pyrosequencing for Detecting Extensively Drug-Resistant Mycobacterium tuberculosis among Clinical Isolates from Four High-Burden Countries

2014 ◽  
Vol 59 (1) ◽  
pp. 414-420 ◽  
Author(s):  
Kanchan Ajbani ◽  
Shou-Yean Grace Lin ◽  
Camilla Rodrigues ◽  
Duylinh Nguyen ◽  
Francine Arroyo ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
The Philippines ◽  
Drug Susceptibility Testing ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Second Line ◽  
Additional Advantage ◽  
Content Type ◽  
Extensively Drug Resistant

ABSTRACTReliable molecular diagnostics, which detect specific mutations associated with drug resistance, are promising technologies for the rapid identification and monitoring of drug resistance inMycobacterium tuberculosisisolates. Pyrosequencing (PSQ) has the ability to detect mutations associated with first- and second-line anti-tuberculosis (TB) drugs, with the additional advantage of being rapidly adaptable for the identification of new mutations. The aim of this project was to evaluate the performance of PSQ in predicting phenotypic drug resistance in multidrug- and extensively drug-resistant tuberculosis (M/XDR-TB) clinical isolates from India, South Africa, Moldova, and the Philippines. A total of 187 archived isolates were run through a PSQ assay in order to identifyM. tuberculosis(via the IS6110marker), and to detect mutations associated with M/XDR-TB within small stretches of nucleotides in selected loci. The molecular targets includedkatG, theinhApromoter and theahpC-oxyRintergenic region for isoniazid (INH) resistance; therpoBcore region for rifampin (RIF) resistance;gyrAfor fluoroquinolone (FQ) resistance; andrrsfor amikacin (AMK), capreomycin (CAP), and kanamycin (KAN) resistance. PSQ data were compared to phenotypic mycobacterial growth indicator tube (MGIT) 960 drug susceptibility testing results for performance analysis. The PSQ assay illustrated good sensitivity for the detection of resistance to INH (94%), RIF (96%), FQ (93%), AMK (84%), CAP (88%), and KAN (68%). The specificities of the assay were 96% for INH, 100% for RIF, FQ, AMK, and KAN, and 97% for CAP. PSQ is a highly efficient diagnostic tool that reveals specific nucleotide changes associated with resistance to the first- and second-line anti-TB drug medications. This methodology has the potential to be linked to mutation-specific clinical interpretation algorithms for rapid treatment decisions.

scholarly journals Lipoprotein Processing Is Essential for Resistance of Mycobacterium tuberculosis to Malachite Green

2009 ◽  
Vol 53 (9) ◽  
pp. 3799-3802 ◽  
Author(s):  
Niaz Banaei ◽  
Eleanor Z. Kincaid ◽  
S.-Y. Grace Lin ◽  
Edward Desmond ◽  
William R. Jacobs ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Malachite Green ◽  
Cell Envelope ◽  
Sodium Dodecyl ◽  
Signal Peptidase ◽  
Wild Type ◽  
First Line ◽  
Green Is ◽  
Antituberculosis Drugs ◽  
Forming Efficiency

ABSTRACT Malachite green, a synthetic antimicrobial dye, has been used for over 50 years in mycobacterial culture medium to inhibit the growth of contaminants. The molecular basis of mycobacterial resistance to malachite green is unknown, although the presence of malachite green-reducing enzymes in the cell envelope has been suggested. The objective of this study was to investigate the role of lipoproteins in resistance of Mycobacterium tuberculosis to malachite green. The replication of an M. tuberculosis lipoprotein signal peptidase II (lspA) mutant (ΔlspA::lspA mut) on Middlebrook agar with and without 1 mg/liter malachite green was investigated. The lspA mutant was also compared with wild-type M. tuberculosis in the decolorization rate of malachite green and sensitivity to sodium dodecyl sulfate (SDS) detergent and first-line antituberculosis drugs. The lspA mutant has a 104-fold reduction in CFU-forming efficiency on Middlebrook agar with malachite green. Malachite green is decolorized faster in the presence of the lspA mutant than wild-type bacteria. The lspA mutant is hypersensitive to SDS detergent and shows increased sensitivity to first-line antituberculosis drugs. In summary, lipoprotein processing by LspA is essential for resistance of M. tuberculosis to malachite green. A cell wall permeability defect is likely responsible for the hypersensitivity of lspA mutant to malachite green.

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