scholarly journals Retrospective analysis of multiplex polymerase chain reaction-based molecular diagnostics (SES) in 70 patients with suspected central nervous system infections: A single-center study

2016 ◽  
Vol 19 (4) ◽  
pp. 482 ◽  
Author(s):  
Rama KrishnanTiruppur Chinnappan Ramalingam ◽  
Dipanjan Chakraborty
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Nervous System ◽  
Multiplex Polymerase Chain Reaction ◽  
Single Center ◽  
Central Nervous System Infections ◽  
Chain Reaction ◽  
Single Center Study ◽  
Polymerase Chain ◽  
Center Study

scholarly journals Effective Use of Polymerase Chain Reaction for Diagnosis of Central Nervous System Infections

1999 ◽  
Vol 29 (4) ◽  
pp. 803-806 ◽  
Author(s):  
Yi‐Wei Tang ◽  
Jonathan R. Hibbs ◽  
Kimberly R. Tau ◽  
Qingfang Qian ◽  
Heather A. Skarhus ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Nervous System ◽  
Central Nervous System Infections ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Effective Use

scholarly journals A Single-center, Quasi-experimental Study to Evaluate the Impact of a Multiplex Polymerase Chain Reaction System Combined with Antimicrobial Stewardship Intervention on Time to Targeted Therapy in Patients with Suspected Central Nervous System Infection

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S8-S8 ◽  
Author(s):  
Kelsey Powell ◽  
Sara Revolinski ◽  
Allison Gibble ◽  
Anne Daniels ◽  
J Njeri Wainaina ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Nervous System ◽  
Targeted Therapy ◽  
Multiplex Polymerase Chain Reaction ◽  
Antimicrobial Agents ◽  
Empiric Therapy ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Research Grant

Abstract Background Empiric treatment for central nervous system (CNS) infections consists of coverage with multiple antimicrobial agents that may be continued until a pathogen can be identified. Identification may take significant time to result, leading to extended durations of multiple antimicrobial agents, delays in targeted therapy and subsequent adverse effects, such as nephrotoxicity and Clostridium difficile infection. A multiplex polymerase chain reaction (PCR) system that can identify 14 pathogens responsible for community-acquired CNS infections in 1 hour was recently FDA-approved for cerebrospinal fluid (CSF) analysis. The objective of this study was to determine the effect of this PCR paired with antimicrobial stewardship (AMS) team intervention on the time to targeted therapy. Methods During the intervention (Int) phase (January 25, 2017–April 30, 2017), all PCR results were called to the AMS team, who reviewed clinical data and provided antimicrobial recommendations per pre-determined protocol. Recommendations consisted of de-escalation or addition of therapy. The pre-intervention (PI) group consisted of patients with CSF culture obtained between January 25, 20116 and April 30, 2016. Results A total of 138 patients were evaluated; 46 in the Int group and 92 in the PI. Of the 46 patients in the Int group, 25 had a negative PCR result and were never initiated on antimicrobials. One patient required antimicrobial escalation. Twenty patients were started on empiric therapy and were candidates for de-escalation. In the PI group, there were no patients with CSF cultures that required therapy escalation, while 33 patients were initiated on empiric antimicrobials. Results from the subgroup of patients in whom empiric therapy was started as shown in Table 1. Conclusion Implementation of a multiplex PCR with AMS intervention resulted in decreased time to targeted therapy. This project was funded through a competitive stewardship grant provided by Merck & Co. Disclosures S. Revolinski, Merck: Grant Investigator, Research grant; J. N. Wainaina, Merck: Grant Investigator, Research grant; A. Huang, Merck: Grant Investigator, Research grant

Laboratory Diagnosis of Pediatric Herpesvirus Infections of the Central Nervous System by a Multiplex Polymerase Chain Reaction Assay and Intrathecal Antibodies

2018 ◽  
Vol 13 (03) ◽  
pp. 178-184
Author(s):  
Sibel Yavru ◽  
Mehmet Özdemir ◽  
Bahadır Feyzioğlu
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Nervous System ◽  
Aseptic Meningitis ◽  
Multiplex Polymerase Chain Reaction ◽  
Viral Infections ◽  
Chain Reaction ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Hsv 1

Abstract Introduction Central nervous system (CNS) viral infections are a serious problem requiring accurate diagnosis and treatment. Human herpesviruses (HHVs) are an important cause of these infections. Recent research has focused on new diagnostic methods allowing accurate and rapid identification of viral infections because there are still diagnostic difficulties for these infections. This study was done to determine the etiologic role of human herpes viruses and to obtain information that will contribute to the diagnostic algorithm in suspected cases of viral encephalitis or aseptic meningitis. Materials and Methods In our study, herpes simplex virus (HSV)-1, HSV-2, varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein–Barr virus (EBV), and HHV-6 DNA was detected in the cerebrospinal fluid (CSF) by multiplex polymerase chain reaction (PCR) and virus-specific immunoglobulin G (IgG) antibodies in CSF and serum by EIA in pediatric encephalitis/meningitis cases. Results HSV-1 and VZV were detected in 5 and 3.3% of aseptic meningitis cases, respectively, but no encephalitis cases. Other viruses were not identified as etiologic agents. The seroprevalences were determined as 72.4, 34.3, 81.9, 93.3, 88.6, and 80.9%, respectively for HSV-1, HSV-2, VZV, CMV, EBV, and HHV-6. The performance of specific IgG CSF/serum antibody index (AI) was not satisfactory. Conclusion Our study indicates that the multiplex PCR method is the most suitable for the diagnosis of CNS infections caused by HHVs. However, due to the high cost of the PCR method, the positive results of the specific AI may be significant, but the negative results are unreliable, especially in limited health care facilities.

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